P10294 (ITR1_MOMCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin inhibitor 1 Alternative name(s): MCTI-I Trypsin inhibitor I
Organism	Momordica charantia (Bitter gourd) (Balsam pear)
Taxonomic identifier	3673 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Momordiceae › Momordica

Protein attributes

Sequence length	30 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits trypsin.
Subcellular location	Secreted.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.
Sequence similarities	Belongs to the protease inhibitor I7 (squash-type serine protease inhibitor) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Domain	Knottin
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	negative regulation of endopeptidase activity Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 30

30

Trypsin inhibitor 1

PRO_0000044386

Sites

Site

6 – 7

2

Reactive bond

Amino acid modifications

Disulfide bond

4 ↔ 21

By similarity

Disulfide bond

11 ↔ 23

By similarity

Disulfide bond

17 ↔ 29

By similarity

Secondary structure

1

.

.

.

.

.

30

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Helix

14 – 16

3

Sequences

	Sequence		Length	Mass (Da)
	P10294 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: D5E1344365E34E9A	FASTA	30	3,413
	10 20 30 ERRCPRILKQ CKRDSDCPGE CICMAHGFCG

References

[1]	"Amino acid sequences and disulfide bridges of serine proteinase inhibitors from bitter gourd (Momordica charantia LINN.) seeds." Hara S., Makino J., Ikenaka T. J. Biochem. 105:88-92(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed.

Cross-references

Sequence databases

PIR

JX0057.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EWU	model	-	I	1-30	[»]

ProteinModelPortal

P10294.

SMR

P10294. Positions 4-30.

ModBase

Search...

Protein family/group databases

MEROPS

I07.001.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]

Pfam

PF00299. Squash. 1 hit.
[Graphical view]

SMART

SM00286. PTI. 1 hit.
[Graphical view]

SUPFAM

SSF57027. Prot_amyl_inhib. 1 hit.

PROSITE

PS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ITR1_MOMCH

Accession

Primary (citable) accession number: P10294

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program