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Protein

Trypsin inhibitor 3

Gene
N/A
Organism
Cucurbita pepo (Vegetable marrow) (Summer squash)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits trypsin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei8 – 92Reactive bond

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI07.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin inhibitor 3
Alternative name(s):
CPTI-III
Trypsin inhibitor III
Cleaved into the following chain:
Alternative name(s):
CPTI-II
Trypsin inhibitor II
OrganismiCucurbita pepo (Vegetable marrow) (Summer squash)
Taxonomic identifieri3663 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3232Trypsin inhibitor 3PRO_0000033199Add
BLAST
Peptidei4 – 3229Trypsin inhibitor 2PRO_0000033200Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi6 ↔ 23By similarity
Disulfide bondi13 ↔ 25By similarity
Disulfide bondi19 ↔ 31By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

MINTiMINT-87977.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 183Combined sources
Beta strandi24 – 263Combined sources
Beta strandi29 – 324Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BTCX-ray1.50I4-32[»]
2STBX-ray1.80I4-32[»]
ProteinModelPortaliP10293.
SMRiP10293. Positions 5-32.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10293.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamiPF00299. Squash. 1 hit.
[Graphical view]
PRINTSiPR00293. SQUASHINHBTR.
SUPFAMiSSF57027. SSF57027. 1 hit.
PROSITEiPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
HEERVCPKIL MECKKDSDCL AECICLEHGY CG
Length:32
Mass (Da):3,656
Last modified:June 30, 1989 - v1
Checksum:i0F6B9820BA13D512
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BTCX-ray1.50I4-32[»]
2STBX-ray1.80I4-32[»]
ProteinModelPortaliP10293.
SMRiP10293. Positions 5-32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-87977.

Protein family/group databases

MEROPSiI07.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP10293.

Family and domain databases

InterProiIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamiPF00299. Squash. 1 hit.
[Graphical view]
PRINTSiPR00293. SQUASHINHBTR.
SUPFAMiSSF57027. SSF57027. 1 hit.
PROSITEiPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The squash family of serine proteinase inhibitors. Amino acid sequences and association equilibrium constants of inhibitors from squash, summer squash, zucchini, and cucumber seeds."
    Wieczorek M., Otlewski J., Cook J., Parks K., Leluk J., Wilimowska-Pelc A., Polanowski A., Wilusz T., Laskowski M. Jr.
    Biochem. Biophys. Res. Commun. 126:646-652(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Seed.

Entry informationi

Entry nameiITR3_CUCPE
AccessioniPrimary (citable) accession number: P10293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1989
Last sequence update: June 30, 1989
Last modified: January 6, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.