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P10293 (ITR3_CUCPE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin inhibitor 3
Alternative name(s):
CPTI-III
Trypsin inhibitor III

Cleaved into the following chain:

  1. Trypsin inhibitor 2
    Alternative name(s):
    CPTI-II
    Trypsin inhibitor II
OrganismCucurbita pepo (Vegetable marrow) (Summer squash)
Taxonomic identifier3663 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Protein attributes

Sequence length32 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits trypsin.

Subcellular location

Secreted.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.

Sequence similarities

Belongs to the protease inhibitor I7 (squash-type serine protease inhibitor) family. [View classification]

Ontologies

Keywords
   Cellular componentSecreted
   DomainKnottin
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 3232Trypsin inhibitor 3
PRO_0000033199
Peptide4 – 3229Trypsin inhibitor 2
PRO_0000033200

Sites

Site8 – 92Reactive bond

Amino acid modifications

Disulfide bond6 ↔ 23 By similarity
Disulfide bond13 ↔ 25 By similarity
Disulfide bond19 ↔ 31 By similarity

Secondary structure

...... 32
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10293 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 0F6B9820BA13D512

FASTA323,656
        10         20         30 
HEERVCPKIL MECKKDSDCL AECICLEHGY CG 

« Hide

References

[1]"The squash family of serine proteinase inhibitors. Amino acid sequences and association equilibrium constants of inhibitors from squash, summer squash, zucchini, and cucumber seeds."
Wieczorek M., Otlewski J., Cook J., Parks K., Leluk J., Wilimowska-Pelc A., Polanowski A., Wilusz T., Laskowski M. Jr.
Biochem. Biophys. Res. Commun. 126:646-652(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Seed.
+Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BTCX-ray1.50I4-32[»]
2STBX-ray1.80I4-32[»]
ProteinModelPortalP10293.
SMRP10293. Positions 5-32.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-87977.

Protein family/group databases

MEROPSI07.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamPF00299. Squash. 1 hit.
[Graphical view]
PRINTSPR00293. SQUASHINHBTR.
SUPFAMSSF57027. SSF57027. 1 hit.
PROSITEPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10293.

Entry information

Entry nameITR3_CUCPE
AccessionPrimary (citable) accession number: P10293
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references