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P10292 (ITR4_CUCSA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin inhibitor 4
Alternative name(s):
CSTI-IV
Trypsin inhibitor IV
OrganismCucumis sativus (Cucumber)
Taxonomic identifier3659 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Protein attributes

Sequence length30 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits trypsin.

Subcellular location

Secreted.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.

Sequence similarities

Belongs to the protease inhibitor I7 (squash-type serine protease inhibitor) family. [View classification]

Ontologies

Keywords
   Cellular componentSecreted
   DomainKnottin
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 3030Trypsin inhibitor 4
PRO_0000044377

Sites

Site5 – 62Reactive bond

Amino acid modifications

Disulfide bond3 ↔ 20 By similarity
Disulfide bond10 ↔ 22 By similarity
Disulfide bond16 ↔ 29 By similarity

Sequences

Sequence LengthMass (Da)Tools
P10292 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 7A4B870E7D2A088F

FASTA303,429
        10         20         30 
MMCPRILMKC KHDSDCLPGC VCLEHIEYCG 

« Hide

References

[1]"The squash family of serine proteinase inhibitors. Amino acid sequences and association equilibrium constants of inhibitors from squash, summer squash, zucchini, and cucumber seeds."
Wieczorek M., Otlewski J., Cook J., Parks K., Leluk J., Wilimowska-Pelc A., Polanowski A., Wilusz T., Laskowski M. Jr.
Biochem. Biophys. Res. Commun. 126:646-652(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Seed.

Cross-references

3D structure databases

ProteinModelPortalP10292.
SMRP10292. Positions 2-30.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI07.015.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamPF00299. Squash. 1 hit.
[Graphical view]
PRINTSPR00293. SQUASHINHBTR.
SUPFAMSSF57027. SSF57027. 1 hit.
PROSITEPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameITR4_CUCSA
AccessionPrimary (citable) accession number: P10292
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families