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P10283

- MTB1_BREEP

UniProt

P10283 - MTB1_BREEP

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Protein

Modification methylase BepI

Gene

bepIM

Organism
Brevibacterium epidermidis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CGCG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BepI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3301. M.BepI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BepI (EC:2.1.1.37)
Short name:
M.BepI
Alternative name(s):
Cytosine-specific methyltransferase BepI
Gene namesi
Name:bepIM
OrganismiBrevibacterium epidermidis
Taxonomic identifieri1698 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeBrevibacteriaceaeBrevibacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Modification methylase BepIPRO_0000087870Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP10283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 400400SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10283-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLSLFSGC GGMDLGLEGG FLAHRSSINS DLYASYISDH DENYVYLKKT
60 70 80 90 100
GFETVFANDI LPFAKLAWCN FFKNRVNQPE NIFHLESIVD VVNNIENKQF
110 120 130 140 150
SFPNDIDVVT GGFPCQDFSF AGKRKGFDSH KDHNGIIYNE PTEATRGQLY
160 170 180 190 200
LWLKKVVEIT KPKVFIAENV KGLVTLGDVK DIIQKDFRNI DDGYVVLDAQ
210 220 230 240 250
VLNAKNYGVA QNRERVIFIG ISKRYANKKI LDELISLQEK SEVYPYPPYT
260 270 280 290 300
HGTDPELKPY ATLNQILAHL PEPELASTDK SQQSYSKAKL FKKTQGNIEV
310 320 330 340 350
NMNGQAPTIR AEHHGNIEFR RLSKENGGTN LSELHLPQRR LTVRECALIQ
360 370 380 390 400
SFPPDYEFVF NYGKANSVSA SAAYKIIGNA VPPLLGFAIG RHLSQIWDKL

FKT
Length:403
Mass (Da):45,466
Last modified:July 1, 1989 - v1
Checksum:i666CEE1C1C69922E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13555 Genomic DNA. Translation: CAA31907.1.
PIRiS02856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13555 Genomic DNA. Translation: CAA31907.1 .
PIRi S02856.

3D structure databases

ProteinModelPortali P10283.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3301. M.BepI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and structure of the BepI modification methylase."
    Kupper D., Zhou J.-G., Venetianer P., Kiss A.
    Nucleic Acids Res. 17:1077-1088(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R/Szeged.

Entry informationi

Entry nameiMTB1_BREEP
AccessioniPrimary (citable) accession number: P10283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 1, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3