Reviewed, UniProtKB/Swiss-Prot P10283 (MTB1_BREEP)

Names and origin

Protein names

Recommended name:
    Modification methylase BepI
      Short name=M.BepI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase BepI

Gene names

Name:

bepIM

Organism

Brevibacterium epidermidis

Taxonomic identifier

1698 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Brevibacteriaceae › Brevibacterium

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CGCG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BepI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Modification methylase BepI		PRO_0000087870
Sites
	Active site	115	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10283-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 666CEE1C1C69922E	FASTA	403	45,466
	10 20 30 40 50 60 MKVLSLFSGC GGMDLGLEGG FLAHRSSINS DLYASYISDH DENYVYLKKT GFETVFANDI 70 80 90 100 110 120 LPFAKLAWCN FFKNRVNQPE NIFHLESIVD VVNNIENKQF SFPNDIDVVT GGFPCQDFSF 130 140 150 160 170 180 AGKRKGFDSH KDHNGIIYNE PTEATRGQLY LWLKKVVEIT KPKVFIAENV KGLVTLGDVK 190 200 210 220 230 240 DIIQKDFRNI DDGYVVLDAQ VLNAKNYGVA QNRERVIFIG ISKRYANKKI LDELISLQEK 250 260 270 280 290 300 SEVYPYPPYT HGTDPELKPY ATLNQILAHL PEPELASTDK SQQSYSKAKL FKKTQGNIEV 310 320 330 340 350 360 NMNGQAPTIR AEHHGNIEFR RLSKENGGTN LSELHLPQRR LTVRECALIQ SFPPDYEFVF 370 380 390 400 NYGKANSVSA SAAYKIIGNA VPPLLGFAIG RHLSQIWDKL FKT

References

[1]	"Cloning and structure of the BepI modification methylase." Kupper D., Zhou J.-G., Venetianer P., Kiss A. Nucleic Acids Res. 17:1077-1088(1989) [PubMed: 2784204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: R/Szeged.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13555 Genomic DNA. Translation: CAA31907.1.
PIR	S02856.
3D structure databases
HSSP	HSSP built from PDB template 1DCT based on UniProtKB P20589.
ModBase	Search...
Protein family/group databases
REBASE	3301. M.BepI.
Enzyme and pathway databases
BRENDA	2.1.1.37. 305167.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BREEP

Accession

Primary (citable) accession number: P10283

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	March 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)