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P10283 (MTB1_BREEP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BepI

Short name=M.BepI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase BepI
Gene names
Name:bepIM
OrganismBrevibacterium epidermidis
Taxonomic identifier1698 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeBrevibacteriaceaeBrevibacterium

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CGCG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BepI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Modification methylase BepI
PRO_0000087870

Regions

Domain1 – 400400SAM-dependent MTase C5-type

Sites

Active site1151 By similarity

Sequences

Sequence LengthMass (Da)Tools
P10283 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 666CEE1C1C69922E

FASTA40345,466
        10         20         30         40         50         60 
MKVLSLFSGC GGMDLGLEGG FLAHRSSINS DLYASYISDH DENYVYLKKT GFETVFANDI 

        70         80         90        100        110        120 
LPFAKLAWCN FFKNRVNQPE NIFHLESIVD VVNNIENKQF SFPNDIDVVT GGFPCQDFSF 

       130        140        150        160        170        180 
AGKRKGFDSH KDHNGIIYNE PTEATRGQLY LWLKKVVEIT KPKVFIAENV KGLVTLGDVK 

       190        200        210        220        230        240 
DIIQKDFRNI DDGYVVLDAQ VLNAKNYGVA QNRERVIFIG ISKRYANKKI LDELISLQEK 

       250        260        270        280        290        300 
SEVYPYPPYT HGTDPELKPY ATLNQILAHL PEPELASTDK SQQSYSKAKL FKKTQGNIEV 

       310        320        330        340        350        360 
NMNGQAPTIR AEHHGNIEFR RLSKENGGTN LSELHLPQRR LTVRECALIQ SFPPDYEFVF 

       370        380        390        400 
NYGKANSVSA SAAYKIIGNA VPPLLGFAIG RHLSQIWDKL FKT 

« Hide

References

[1]"Cloning and structure of the BepI modification methylase."
Kupper D., Zhou J.-G., Venetianer P., Kiss A.
Nucleic Acids Res. 17:1077-1088(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: R/Szeged.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13555 Genomic DNA. Translation: CAA31907.1.
PIRS02856.

3D structure databases

ProteinModelPortalP10283.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3301. M.BepI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB1_BREEP
AccessionPrimary (citable) accession number: P10283
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries