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Protein

Modification methylase BepI

Gene

bepIM

Organism
Brevibacterium epidermidis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CGCG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BepI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3301. M.BepI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BepI (EC:2.1.1.37)
Short name:
M.BepI
Alternative name(s):
Cytosine-specific methyltransferase BepI
Gene namesi
Name:bepIM
OrganismiBrevibacterium epidermidis
Taxonomic identifieri1698 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesBrevibacteriaceaeBrevibacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Modification methylase BepIPRO_0000087870Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP10283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 400400SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLSLFSGC GGMDLGLEGG FLAHRSSINS DLYASYISDH DENYVYLKKT
60 70 80 90 100
GFETVFANDI LPFAKLAWCN FFKNRVNQPE NIFHLESIVD VVNNIENKQF
110 120 130 140 150
SFPNDIDVVT GGFPCQDFSF AGKRKGFDSH KDHNGIIYNE PTEATRGQLY
160 170 180 190 200
LWLKKVVEIT KPKVFIAENV KGLVTLGDVK DIIQKDFRNI DDGYVVLDAQ
210 220 230 240 250
VLNAKNYGVA QNRERVIFIG ISKRYANKKI LDELISLQEK SEVYPYPPYT
260 270 280 290 300
HGTDPELKPY ATLNQILAHL PEPELASTDK SQQSYSKAKL FKKTQGNIEV
310 320 330 340 350
NMNGQAPTIR AEHHGNIEFR RLSKENGGTN LSELHLPQRR LTVRECALIQ
360 370 380 390 400
SFPPDYEFVF NYGKANSVSA SAAYKIIGNA VPPLLGFAIG RHLSQIWDKL

FKT
Length:403
Mass (Da):45,466
Last modified:July 1, 1989 - v1
Checksum:i666CEE1C1C69922E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13555 Genomic DNA. Translation: CAA31907.1.
PIRiS02856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13555 Genomic DNA. Translation: CAA31907.1.
PIRiS02856.

3D structure databases

ProteinModelPortaliP10283.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3301. M.BepI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and structure of the BepI modification methylase."
    Kupper D., Zhou J.-G., Venetianer P., Kiss A.
    Nucleic Acids Res. 17:1077-1088(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R/Szeged.

Entry informationi

Entry nameiMTB1_BREEP
AccessioniPrimary (citable) accession number: P10283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 24, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.