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P10282 (RNF1_GIBFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanyl-specific ribonuclease F1

Short name=RNase F1
EC=3.1.27.3
OrganismGibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
Taxonomic identifier5127 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium fujikuroi species complex

Protein attributes

Sequence length131 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sequence similarities

Belongs to the ribonuclease N1/T1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.2 Ref.3
Chain26 – 131106Guanyl-specific ribonuclease F1
PRO_0000030834

Sites

Active site651
Active site831Proton acceptor
Active site1161Proton donor

Amino acid modifications

Modified residue261Pyrrolidone carboxylic acid
Disulfide bond31 ↔ 127 Ref.3
Disulfide bond49 ↔ 108 Ref.3

Experimental info

Sequence conflict571T → S AA sequence Ref.2
Sequence conflict571T → S AA sequence Ref.3
Sequence conflict611S → T AA sequence Ref.2
Sequence conflict611S → T AA sequence Ref.3
Sequence conflict129 – 1313GTN → NTG AA sequence Ref.2

Secondary structure

.................... 131
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10282 [UniParc].

Last modified August 31, 2004. Version 4.
Checksum: 4AD743FA7E042284

FASTA13113,606
        10         20         30         40         50         60 
MLFFKSIASL AALVSLAVAS PIESRQSATT CGSTNYSASQ VRAAANAACQ YYQNDDTAGS 

        70         80         90        100        110        120 
STYPHTYNNY EGFDFPVDGP YQEFPIKSGG VYTGGSPGAD RVVINTNCEY AGAITHTGAS 

       130 
GNNFVGCSGT N 

« Hide

References

[1]"Cloning and sequencing of cDNA encoding ribonuclease F1 from Fusarium moniliforme."
Yoshida H., Iizuka M., Norioka N., Norioka S., Sakiyama F.
Biochem. Mol. Biol. Int. 45:555-560(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The primary structure of ribonuclease F1 from Fusarium moniliforme."
Hirabayashi J., Yoshida H.
Biochem. Int. 7:255-262(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-131.
[3]"Peptide fractionation by high-performance liquid chromatography on an Asahipak GS-320 column: application to determination of the disulfide pairings in ribonuclease F1."
Yoshida H., Naijo S.
Anal. Biochem. 159:273-279(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-131, DISULFIDE BONDS.
[4]"Crystal structures of ribonuclease F1 of Fusarium moniliforme in its free form and in complex with 2'GMP."
Vassylyev D.G., Katayanagi K., Ishikawa K., Tsujimoto-Hirano M., Danno M., Paehler A., Matsumoto O., Matsushima M., Yoshida H., Morikawa K.
J. Mol. Biol. 230:979-996(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 26-131.
[5]"The three-dimensional structure of guanine-specific ribonuclease F1 in solution determined by NMR spectroscopy and distance geometry."
Nakai T., Yoshikawa W., Nakamura H., Yoshida H.
Eur. J. Biochem. 208:41-51(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 26-131.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006460 mRNA. Translation: BAA31984.1.
PIRJS0484.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FUSX-ray1.30A27-131[»]
1FUTX-ray2.00A27-131[»]
1RCKNMR-A27-131[»]
1RCLNMR-A27-131[»]
ProteinModelPortalP10282.
SMRP10282. Positions 26-131.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.450.30. 1 hit.
InterProIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMSSF53933. SSF53933. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP10282.

Entry information

Entry nameRNF1_GIBFU
AccessionPrimary (citable) accession number: P10282
Secondary accession number(s): O74124
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 31, 2004
Last modified: May 14, 2014
This is version 96 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references