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Protein

Endogenous retrovirus group K member 10 Pol protein

Gene

ERVK-10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation
Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri587 – 62842Integrase-typePROSITE-ProRule annotationAdd
BLAST
DNA bindingi811 – 85949Integrase-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endogenous retrovirus group K member 10 Pol protein
Alternative name(s):
HERV-K10 Pol protein
HERV-K107 Pol protein
HERV-K_5q33.3 provirus ancestral Pol protein
Including the following 3 domains:
Reverse transcriptase (EC:2.7.7.49)
Short name:
RT
Ribonuclease H (EC:3.1.26.4)
Short name:
RNase H
Integrase
Short name:
IN
Gene namesi
Name:ERVK-10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:39004. ERVK-10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10141014Endogenous retrovirus group K member 10 Pol proteinPRO_0000186769Add
BLAST

Proteomic databases

PRIDEiP10266.

Interactioni

Protein-protein interaction databases

IntActiP10266. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP10266.
SMRiP10266. Positions 36-855.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 245189Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini460 – 589130RNase HPROSITE-ProRule annotationAdd
BLAST
Domaini642 – 803162Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi161 – 1644LPQG
Motifi195 – 1984YXDD

Domaini

The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.

Sequence similaritiesi

Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
Contains 1 integrase-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 integrase-type zinc finger.PROSITE-ProRule annotation
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
Contains 1 RNase H domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri587 – 62842Integrase-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG053630.
PhylomeDBiP10266.

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR029104. HERV-K_env.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010661. RVT_thumb.
[Graphical view]
PfamiPF13804. HERV-K_env_2. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00078. RVT_1. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10266-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
NKSRKRRNRV SFLGAVTVEP PKPIPLTWKT EKPVWVNQWP LPKQKLEALH
60 70 80 90 100
LLANEQLEKG HIEPSFSPWN SPVFVIQKKS GKWHTLTDLR AVNAVIQPMG
110 120 130 140 150
PLQPGLPSPA MIPKDWPLII IDLKDCFFTI PLAEQDCEKF AFTIPAINNK
160 170 180 190 200
EPATRFQWKV LPQGMLNSPT ICQTFVGRAL QPVREKFSDC YIIHYIDDIL
210 220 230 240 250
CAAETKDKLI DCYTFLQAEV ANAGLAIASD KIQTSTPFHY LGMQIENRKI
260 270 280 290 300
KPQKIEIRKD TLKTLNDFQK LLGDINWIRP TLGIPTYAMS NLFSILRGDS
310 320 330 340 350
DLNSQRILTP EATKEIKLVE EKIQSAQINR IDPLAPLQLL IFATAHSPTG
360 370 380 390 400
IIIQNTDLVE WSFLPHSTVK TFTLYLDQIA TLIGQTRLRI TKLCGNDPDK
410 420 430 440 450
IVVPLTKEQV RQAFINSGAW QIGLANFVGL IDNHYPKTKI FQFLKLTTWI
460 470 480 490 500
LPKITRREPL ENALTVFTDG SSNGKAAYTG PKERVIKTPY QSAQRDELVA
510 520 530 540 550
VITVLQDFDQ PINIISDSAY VVQATRDVET ALIKYSMDDQ LNQLFNLLQQ
560 570 580 590 600
TVRKRNFPFY ITYIRAHTNL PGPLTKANEQ ADLLVSSALI KAQELHALTH
610 620 630 640 650
VNAAGLKNKF DVTWKQAKDI VQHCTQCQVL HLPTQEAGVN PRGLCPNALW
660 670 680 690 700
QMDVTHVPSF GRLSYVHVTV DTYSHFIWAT CQTGESTSHV KKHLLSCFAV
710 720 730 740 750
MGVPEKIKTD NGPGYCSKAF QKFLSQWKIS HTTGIPYNSQ GQAIVERTNR
760 770 780 790 800
TLKTQLVKQK EGGDSKECTT PQMQLNLALY TLNFLNIYRN QTTTSAEQHL
810 820 830 840 850
TGKKNSPHEG KLIWWKDNKN KTWEIGKVIT WGRGFACVSP GENQLPVWLP
860 870 880 890 900
TRHLKFYNEP IGDAKKRAST EMVTPVTWMD NPIEVYVNDS IWVPGPIDDR
910 920 930 940 950
CPAKPEEEGM MINISIGYRY PPICLGRAPG CLMPAVQNWL VEVPTVSPIS
960 970 980 990 1000
RFTYHMVSGM SLRPRVNYLQ DFSYQRSLKF RPKGKPCPKE IPKESKNTEV
1010
LVWEECVANS AVIL
Length:1,014
Mass (Da):114,827
Last modified:September 13, 2004 - v2
Checksum:i7E320F8F5DEE19FE
GO

Sequence cautioni

The sequence AAD51796.1 differs from that shown. Reason: Frameshift at several positions. A -1 frameshift presumed to occur at the N-terminus at the Pro-Pol gene boundary.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11N → M (PubMed:3021993).Curated
Sequence conflicti496 – 4961D → A (PubMed:9217052).Curated
Sequence conflicti496 – 4961D → A (PubMed:10469592).Curated
Sequence conflicti563 – 5631Y → H in AAD51796 (PubMed:10469592).Curated
Sequence conflicti674 – 6741S → SYS (PubMed:10469592).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016577 Genomic DNA. No translation available.
M14123 Genomic DNA. Translation: AAA88033.1. Sequence problems.
Y10391 Genomic DNA. Translation: CAA71417.1.
AF164613 Genomic DNA. Translation: AAD51796.1. Sequence problems.
PIRiD24483. GNHUER.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016577 Genomic DNA. No translation available.
M14123 Genomic DNA. Translation: AAA88033.1. Sequence problems.
Y10391 Genomic DNA. Translation: CAA71417.1.
AF164613 Genomic DNA. Translation: AAD51796.1. Sequence problems.
PIRiD24483. GNHUER.

3D structure databases

ProteinModelPortaliP10266.
SMRiP10266. Positions 36-855.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10266. 1 interaction.

Proteomic databases

PRIDEiP10266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GeneCardsiERVK-10.
H-InvDBHIX0160040.
HIX0160041.
HIX0164419.
HGNCiHGNC:39004. ERVK-10.
neXtProtiNX_P10266.
GenAtlasiSearch...

Phylogenomic databases

HOVERGENiHBG053630.
PhylomeDBiP10266.

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR029104. HERV-K_env.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010661. RVT_thumb.
[Graphical view]
PfamiPF13804. HERV-K_env_2. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00078. RVT_1. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Nucleotide sequence of human endogenous retrovirus genome related to the mouse mammary tumor virus genome."
    Ono M., Yasunaga T., Miyata T., Ushikubo H.
    J. Virol. 60:589-598(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-740.
  3. "Characterization of human endogenous retrovirus type K (HERV-K) virus-like particles generated from recombinant baculoviruses."
    Toenjes R.R., Boller K., Limbach R., Lugert R., Kurth R.
    Virology 233:280-291(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-740.
  4. "Many human endogenous retrovirus K (HERV-K) proviruses are unique to humans."
    Barbulescu M., Turner G., Seaman M.I., Deinard A.S., Kidd K.K., Lenz J.
    Curr. Biol. 9:861-868(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-830.

Entry informationi

Entry nameiPOK10_HUMAN
AccessioniPrimary (citable) accession number: P10266
Secondary accession number(s): P87890, Q14273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: September 13, 2004
Last modified: October 14, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries.
Has a type 1 genome. The HERV-K(HML-2) family contains type 1 and type 2 genomes depending on the absence or presence of 292 nucleotides at the 5'-end of the env gene. Type 1 genomes lack a pol stop codon, leading to expression of a fusion protein containing a portion of the Env sequence.
Exact N-terminus of this protein has not been formally described.

Keywords - Technical termi

Complete proteome, ERV, Multifunctional enzyme, Reference proteome, Transposable element

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.