Reviewed, UniProtKB/Swiss-Prot P10266 (POK10_HUMAN)

Names and origin

Protein names	Recommended name:     HERV-K_5q33.3 provirus ancestral Pol protein Alternative name(s):     HERV-K10 Pol protein     HERV-K107 Pol protein Including the following 3 domains:     1- Recommended name:             Reverse transcriptase                 Short name=RT               EC=2.7.7.49     2- Recommended name:             Ribonuclease H                 Short name=RNase H               EC=3.1.26.4     3- Recommended name:             Integrase                 Short name=IN
Organism	Homo sapiens (Human) [Complete proteome]
Taxonomic identifier	9606 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1014 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Endonucleolytic cleavage to 5'-phosphomonoester.
Domain	The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.
Miscellaneous	This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries. HERV-K_5q33.3 has a type 1 genome. The HERV-K(HML-2) family contains type 1 and type 2 genomes depending on the absence or presence of 292 nucleotides at the 5'-end of the env gene. Type 1 genomes lack a pol stop codon, leading to expression of a fusion protein containing a portion of the Env sequence. Exact N-terminus of this protein has not been formally described.
Sequence similarities	Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) subfamily. Contains 1 integrase catalytic domain. Contains 1 integrase-type DNA-binding domain. Contains 1 integrase-type zinc finger. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.
Sequence caution	The sequence AAD51796.1 differs from that shown. Reason: Frameshift at several positions. A -1 frameshift presumed to occur at the N-terminus at the Pro-Pol gene boundary.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Complete proteome ERV Multifunctional enzyme Transposable element
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Non-traceable author statement. Source: UniProtKB integrase activity Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1014	1014	HERV-K_5q33.3 provirus ancestral Pol protein		PRO_0000186769
Regions
	Domain	57 – 245	189	Reverse transcriptase
	Domain	460 – 589	130	RNase H
	Domain	642 – 803	162	Integrase catalytic
	Zinc finger	587 – 628	42	Integrase-type
	DNA binding	811 – 859	49	Integrase-type
	Motif	161 – 164	4	LPQG
	Motif	195 – 198	4	YXDD
Experimental info
	Sequence conflict	1	1	N → M Ref.2
	Sequence conflict	496	1	D → A Ref.3
	Sequence conflict	496	1	D → A Ref.4
	Sequence conflict	563	1	Y → H in AAD51796. Ref.4
	Sequence conflict	674	1	S → SYS Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P10266-1 [UniParc]. Last modified September 13, 2004. Version 2. Checksum: 7E320F8F5DEE19FE	FASTA	1,014	114,827
	10 20 30 40 50 60 NKSRKRRNRV SFLGAVTVEP PKPIPLTWKT EKPVWVNQWP LPKQKLEALH LLANEQLEKG 70 80 90 100 110 120 HIEPSFSPWN SPVFVIQKKS GKWHTLTDLR AVNAVIQPMG PLQPGLPSPA MIPKDWPLII 130 140 150 160 170 180 IDLKDCFFTI PLAEQDCEKF AFTIPAINNK EPATRFQWKV LPQGMLNSPT ICQTFVGRAL 190 200 210 220 230 240 QPVREKFSDC YIIHYIDDIL CAAETKDKLI DCYTFLQAEV ANAGLAIASD KIQTSTPFHY 250 260 270 280 290 300 LGMQIENRKI KPQKIEIRKD TLKTLNDFQK LLGDINWIRP TLGIPTYAMS NLFSILRGDS 310 320 330 340 350 360 DLNSQRILTP EATKEIKLVE EKIQSAQINR IDPLAPLQLL IFATAHSPTG IIIQNTDLVE 370 380 390 400 410 420 WSFLPHSTVK TFTLYLDQIA TLIGQTRLRI TKLCGNDPDK IVVPLTKEQV RQAFINSGAW 430 440 450 460 470 480 QIGLANFVGL IDNHYPKTKI FQFLKLTTWI LPKITRREPL ENALTVFTDG SSNGKAAYTG 490 500 510 520 530 540 PKERVIKTPY QSAQRDELVA VITVLQDFDQ PINIISDSAY VVQATRDVET ALIKYSMDDQ 550 560 570 580 590 600 LNQLFNLLQQ TVRKRNFPFY ITYIRAHTNL PGPLTKANEQ ADLLVSSALI KAQELHALTH 610 620 630 640 650 660 VNAAGLKNKF DVTWKQAKDI VQHCTQCQVL HLPTQEAGVN PRGLCPNALW QMDVTHVPSF 670 680 690 700 710 720 GRLSYVHVTV DTYSHFIWAT CQTGESTSHV KKHLLSCFAV MGVPEKIKTD NGPGYCSKAF 730 740 750 760 770 780 QKFLSQWKIS HTTGIPYNSQ GQAIVERTNR TLKTQLVKQK EGGDSKECTT PQMQLNLALY 790 800 810 820 830 840 TLNFLNIYRN QTTTSAEQHL TGKKNSPHEG KLIWWKDNKN KTWEIGKVIT WGRGFACVSP 850 860 870 880 890 900 GENQLPVWLP TRHLKFYNEP IGDAKKRAST EMVTPVTWMD NPIEVYVNDS IWVPGPIDDR 910 920 930 940 950 960 CPAKPEEEGM MINISIGYRY PPICLGRAPG CLMPAVQNWL VEVPTVSPIS RFTYHMVSGM 970 980 990 1000 1010 SLRPRVNYLQ DFSYQRSLKF RPKGKPCPKE IPKESKNTEV LVWEECVANS AVIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Nucleotide sequence of human endogenous retrovirus genome related to the mouse mammary tumor virus genome." Ono M., Yasunaga T., Miyata T., Ushikubo H. J. Virol. 60:589-598(1986) [PubMed: 3021993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-740.
[3]	"Characterization of human endogenous retrovirus type K (HERV-K) virus-like particles generated from recombinant baculoviruses." Toenjes R.R., Boller K., Limbach R., Lugert R., Kurth R. Virology 233:280-291(1997) [PubMed: 9217052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-740.
[4]	"Many human endogenous retrovirus K (HERV-K) proviruses are unique to humans." Barbulescu M., Turner G., Seaman M.I., Deinard A.S., Kidd K.K., Lenz J. Curr. Biol. 9:861-868(1999) [PubMed: 10469592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-830.

Web resources

Human Endogenous Retrovirus Database (HERVd)

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC016577 Genomic DNA. No translation available. M14123 Genomic DNA. Translation: AAA88033.1. Sequence problems. Y10391 Genomic DNA. Translation: CAA71417.1. AF164613 Genomic DNA. Translation: AAD51796.1. Sequence problems.
IPI	IPI00382531.
PIR	GNHUER. D24483.
3D structure databases
SMR	P10266. Positions 24-423, 589-629, 643-785.
ModBase	Search...
Protein-protein interaction databases
STRING	P10266.
Proteomic databases
PRIDE	P10266.
Organism-specific databases
H-InvDB	HIX0056908. HIX0057602.
Phylogenomic databases
HOVERGEN	P10266.
Enzyme and pathway databases
BRENDA	2.7.7.49. 247. 3.1.26.4. 247.
Gene expression databases
Genevestigator	P10266.
Family and domain databases
InterPro	IPR001037. Integrase_C_retrovir. IPR001584. Integrase_cat-core. IPR003308. Integrase_Zn-bd_dom_N. IPR012337. PolynucTfrase_RNaseH_fold. IPR000477. Reverse_transcriptase. IPR002156. RNase_H. IPR010661. RVT_thumb. [Graphical view]
Pfam	PF00552. Integrase. 1 hit. PF02022. Integrase_Zn. 1 hit. PF00075. RnaseH. 1 hit. PF00665. rve. 1 hit. PF00078. RVT_1. 1 hit. PF06817. RVT_thumb. 1 hit. [Graphical view]
PROSITE	PS50994. INTEGRASE. 1 hit. PS51027. INTEGRASE_DBD. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. PS50876. ZF_INTEGRASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POK10_HUMAN

Accession

Primary (citable) accession number: P10266
Secondary accession number(s): P87890, Q14273

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	September 13, 2004
Last modified:	January 19, 2010
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.