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P10262

- GAG_FLV

UniProt

P10262 - GAG_FLV

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Protein

Gag polyprotein

Gene

gag

Organism
Feline leukemia virus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei201 – 2022Cleavage; by viral proteaseBy similarity
Sitei271 – 2722Cleavage; by viral proteaseBy similarity
Sitei519 – 5202Cleavage; by viral proteaseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri547 – 56418CCHC-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiFeline leukemia virus
Taxonomic identifieri11768 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiFelidae (cat family) [TaxID: 9681]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Gag polyproteinPRO_0000390798Add
BLAST
Propeptidei1 – 7474Leader peptideSequence AnalysisPRO_0000040836Add
BLAST
Chaini75 – 201127Matrix protein p15Sequence AnalysisPRO_0000040837Add
BLAST
Chaini202 – 27170RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040838Add
BLAST
Chaini272 – 519248Capsid protein p30Sequence AnalysisPRO_0000040839Add
BLAST
Chaini520 – 58061Nucleocapsid protein p10Sequence AnalysisPRO_0000040840Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi76 – 761N-myristoyl glycine; by hostBy similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP10262.
SMRiP10262. Positions 76-172, 272-402, 408-438, 537-567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi192 – 1954PTAP/PSAP motif
Motifi231 – 2344PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 (By similarity).By similarity

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri547 – 56418CCHC-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10262-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGASSGTAI GAHLFGVSPE YRVLIGDGGA GPSKSLSEVS FSVWYRSRAA
60 70 80 90 100
RLVILCLVAS FLVPCLTFLI AEAVMGQTVT TPLSLTLDHW SEVRARAHNQ
110 120 130 140 150
GVEVRKKKWI TLCEAEWVMM NVGWPREGTF SLDNISQVEK KIFAPGPHGH
160 170 180 190 200
PDQVPYITTW RSLATDPPSW VRPFLPPPKP PTPLPQPLSP QPSAPLTSSL
210 220 230 240 250
YPVVPKPDPP KPPVLPPDPS SPLIDLLTEE PPPYPGGHGP PPSGPRTPAA
260 270 280 290 300
SPIASRLRER RENPAEESQA LPLREGPNNR PQYWPFSASD LYNWKSHNPP
310 320 330 340 350
FSQDPVALTN LIESILVTHQ PTWDDCQQLL QALLTGEERQ RVLLEARKQV
360 370 380 390 400
PGEDGRPTQL PNVIDETFPL TRPNWDFATP AGREHLRLYR QLLLAGLRGA
410 420 430 440 450
ARRPTNLAQV KQVVQGKEET PAAFLERLKE AYRMYTPYDP EDPGQAASVI
460 470 480 490 500
LSFIYQSSPD IRNKLQRLEG LQGFTLSDLL KEAEKIYNKR ETPEEREERL
510 520 530 540 550
WQRQEERDKK RHKEMTKVLA TVVAQNRDKD REENKLGDQR KIPLGKDQCA
560 570 580
YCKEKGHWVR DCPKRPRKKP ANSTLLNLED
Length:580
Mass (Da):65,195
Last modified:July 1, 1989 - v1
Checksum:i10F8C3775B37042C
GO

Sequence cautioni

The sequence AAA43055.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01803 Genomic RNA. Translation: AAA43054.1.
K01803 Genomic RNA. Translation: AAA43055.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01803 Genomic RNA. Translation: AAA43054.1 .
K01803 Genomic RNA. Translation: AAA43055.1 . Different initiation.

3D structure databases

ProteinModelPortali P10262.
SMRi P10262. Positions 76-172, 272-402, 408-438, 537-567.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEi PS50158. ZF_CCHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the gag gene and gag-pol junction of feline leukemia virus."
    Laprevotte I., Hampe A., Sherr C.J., Galibert F.
    J. Virol. 50:884-894(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiGAG_FLV
AccessioniPrimary (citable) accession number: P10262
Secondary accession number(s): Q85560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3