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P10262 (GAG_FLV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein

Cleaved into the following 4 chains:

  1. Matrix protein p15
    Short name=MA
  2. RNA-binding phosphoprotein p12
    Alternative name(s):
    pp12
  3. Capsid protein p30
    Short name=CA
  4. Nucleocapsid protein p10
    Short name=NC-gag
Gene names
Name:gag
OrganismFeline leukemia virus
Taxonomic identifier11768 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostFelidae (cat family) [TaxID: 9681]

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.

Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.

Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.

Subunit structure

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Subcellular location

Gag polyprotein: Virion By similarity. Host cell membrane; Lipid-anchor Potential.

Matrix protein p15: Virion Potential.

Capsid protein p30: Virion Potential.

Nucleocapsid protein p10: Virion Potential.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 By similarity.

Post-translational modification

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Sequence similarities

Contains 1 CCHC-type zinc finger.

Sequence caution

The sequence AAA43055.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580Gag polyprotein
PRO_0000390798
Propeptide1 – 7474Leader peptide Potential
PRO_0000040836
Chain75 – 201127Matrix protein p15 Potential
PRO_0000040837
Chain202 – 27170RNA-binding phosphoprotein p12 Potential
PRO_0000040838
Chain272 – 519248Capsid protein p30 Potential
PRO_0000040839
Chain520 – 58061Nucleocapsid protein p10 Potential
PRO_0000040840

Regions

Zinc finger547 – 56418CCHC-type
Motif192 – 1954PTAP/PSAP motif
Motif231 – 2344PPXY motif

Sites

Site201 – 2022Cleavage; by viral protease By similarity
Site271 – 2722Cleavage; by viral protease By similarity
Site519 – 5202Cleavage; by viral protease By similarity

Amino acid modifications

Lipidation761N-myristoyl glycine; by host By similarity

Sequences

Sequence LengthMass (Da)Tools
P10262 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 10F8C3775B37042C

FASTA58065,195
        10         20         30         40         50         60 
MSGASSGTAI GAHLFGVSPE YRVLIGDGGA GPSKSLSEVS FSVWYRSRAA RLVILCLVAS 

        70         80         90        100        110        120 
FLVPCLTFLI AEAVMGQTVT TPLSLTLDHW SEVRARAHNQ GVEVRKKKWI TLCEAEWVMM 

       130        140        150        160        170        180 
NVGWPREGTF SLDNISQVEK KIFAPGPHGH PDQVPYITTW RSLATDPPSW VRPFLPPPKP 

       190        200        210        220        230        240 
PTPLPQPLSP QPSAPLTSSL YPVVPKPDPP KPPVLPPDPS SPLIDLLTEE PPPYPGGHGP 

       250        260        270        280        290        300 
PPSGPRTPAA SPIASRLRER RENPAEESQA LPLREGPNNR PQYWPFSASD LYNWKSHNPP 

       310        320        330        340        350        360 
FSQDPVALTN LIESILVTHQ PTWDDCQQLL QALLTGEERQ RVLLEARKQV PGEDGRPTQL 

       370        380        390        400        410        420 
PNVIDETFPL TRPNWDFATP AGREHLRLYR QLLLAGLRGA ARRPTNLAQV KQVVQGKEET 

       430        440        450        460        470        480 
PAAFLERLKE AYRMYTPYDP EDPGQAASVI LSFIYQSSPD IRNKLQRLEG LQGFTLSDLL 

       490        500        510        520        530        540 
KEAEKIYNKR ETPEEREERL WQRQEERDKK RHKEMTKVLA TVVAQNRDKD REENKLGDQR 

       550        560        570        580 
KIPLGKDQCA YCKEKGHWVR DCPKRPRKKP ANSTLLNLED 

« Hide

References

[1]"Nucleotide sequence of the gag gene and gag-pol junction of feline leukemia virus."
Laprevotte I., Hampe A., Sherr C.J., Galibert F.
J. Virol. 50:884-894(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K01803 Genomic RNA. Translation: AAA43054.1.
K01803 Genomic RNA. Translation: AAA43055.1. Different initiation.

3D structure databases

ProteinModelPortalP10262.
SMRP10262. Positions 76-172, 272-402, 408-438, 537-567.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAG_FLV
AccessionPrimary (citable) accession number: P10262
Secondary accession number(s): Q85560
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families