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Protein

Gag polyprotein

Gene

gag

Organism
Feline leukemia virus
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Gag polyprotein: Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.By similarity
Matrix protein p15: Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.By similarity
RNA-binding phosphoprotein p12: Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.By similarity
Capsid protein p30: Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10-Gag: Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri473 – 490CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Gene namesi
Name:gag
OrganismiFeline leukemia virus
Taxonomic identifieri11768 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiFelidae (cat family) [TaxID: 9681]
Proteomesi
  • UP000118006 Componenti: Genome

Subcellular locationi

Gag polyprotein :
  • Virion By similarity
  • Host cell membrane By similarity; Lipid-anchor By similarity
  • Host late endosome membrane By similarity; Lipid-anchor By similarity
  • host multivesicular body By similarity
  • Note: These locations are probably linked to virus assembly sites.Curated
Matrix protein p15 :
  • Virion By similarity
Capsid protein p30 :
  • Virion By similarity
Nucleocapsid protein p10-Gag :
  • Virion By similarity
RNA-binding phosphoprotein p12 :
  • Host cytoplasm By similarity
  • Note: Localizes to the host cytoplasm early in infection and binds to the mitotic chromosomes later on.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host cytoplasm, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostSequence analysis
ChainiPRO_00003907982 – 506Gag polyproteinAdd BLAST505
ChainiPRO_00000408372 – 127Matrix protein p15Add BLAST126
ChainiPRO_0000040838128 – 197RNA-binding phosphoprotein p12Add BLAST70
ChainiPRO_0000040839198 – 445Capsid protein p30Add BLAST248
ChainiPRO_0000040840446 – 506Nucleocapsid protein p10-GagAdd BLAST61

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostSequence analysis1

Post-translational modificationi

Gag polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation.By similarity
RNA-binding phosphoprotein p12 is phosphorylated on serine residues.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei127 – 128Cleavage; by viral proteaseBy similarity2
Sitei197 – 198Cleavage; by viral proteaseBy similarity2
Sitei445 – 446Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Interactioni

Subunit structurei

Capsid protein p30: Homohexamer; further associates as homomultimer (By similarity). Capsid protein p30: The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity). Gag polyprotein: Interacts (via PPXY motif) with host NEDD4 (By similarity). Gag polyprotein: Interacts (via PSAP motif) with host TSG101 (By similarity). Gag polyprotein: Interacts (via LYPX(n)L motif) with host PDCD6IP (By similarity).PROSITE-ProRule annotationBy similarity

Structurei

3D structure databases

ProteinModelPortaliP10262
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi118 – 121PTAP/PSAP motifBy similarity4
Motifi126 – 130LYPX(n)L motifBy similarity5
Motifi157 – 160PPXY motifBy similarity4

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. PPXY motif is essential for virus egress. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L motif which potentially interacts with PDCD6IP. Both PSAP and LYPX(n)L domains might play little to no role in budding and possibly drive residual virus release. contains.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri473 – 490CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.150.180, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR000840 G_retro_matrix
IPR036946 G_retro_matrix_sf
IPR002079 Gag_p12
IPR003036 Gag_P30
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF01140 Gag_MA, 1 hit
PF01141 Gag_p12, 1 hit
PF02093 Gag_p30, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 1 hit
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Gag polyprotein (identifier: P10262-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTITTPLS LTLDHWSEVR ARAHNQGVEV RKKKWITLCE AEWVMMNVGW
60 70 80 90 100
PREGTFSLDN ISQVEKKIFA PGPYGHPDQV PYITTWRSLA TDPPSWVRPF
110 120 130 140 150
LPPPKPPTPL PQPLSPQPSA PLTSSLYPVL PKSDPPKPPV LPPDPSSPLI
160 170 180 190 200
DLLTEEPPPY PGGHGPPPSG PRTPTASPIA SRLRERRENP AEESQALPLR
210 220 230 240 250
EGPNNRPQYW PFSASDLYNW KSHNPPFSQD PVALTNLIES ILVTHQPTWD
260 270 280 290 300
DCQQLLQALL TGEERQRVLL EARKQVPGED GRPTQLPNVI DETFPLTRPN
310 320 330 340 350
WDFATPAGRE HLRLYRQLLL AGLRGAARRP TNLAQVKQVV QGKEETPAAF
360 370 380 390 400
LERLKEAYRM YTPYDPEDPG QAASVILSFI YQSSPDIRNK LQRLEGLQGF
410 420 430 440 450
TLSDLLKEAE KIYNKRETPE EREERLWQRQ EERDKKRHKE MTKVLATVVA
460 470 480 490 500
QNRDKDREES KLGDQRKIPL GKDQCAYCKE KGHWVRDCPK RPRKKPANST

LLNLGD
Length:506
Mass (Da):57,527
Last modified:January 31, 2018 - v3
Checksum:iD18569C69A6DEF6F
GO
Isoform Glyco-Gag protein (identifier: P0DOH3-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DOH3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:580
Mass (Da):65,195
GO

Sequence cautioni

The sequence AAA43054 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti5I → V1 Publication1
Natural varianti74Y → H1 Publication1
Natural varianti130L → V1 Publication1
Natural varianti133S → P1 Publication1
Natural varianti175T → A1 Publication1
Natural varianti460S → N1 Publication1
Natural varianti505G → E1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052723 Genomic DNA No translation available.
K01803 Genomic RNA Translation: AAA43054.1 Different initiation.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiGAG_FLV
AccessioniPrimary (citable) accession number: P10262
Secondary accession number(s): Q85560
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 31, 2018
Last modified: March 28, 2018
This is version 97 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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