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Protein

Gag polyprotein

Gene

gag

Organism
Mouse mammary tumor virus (strain BR6) (MMTV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Matrix protein p10: Matrix protein.Curated
Nucleocapsid protein p14: Binds strongly to viral nucleic acids and promotes their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.Curated
Capsid protein p27: Capsid protein.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri525 – 542CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri552 – 569CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Gene namesi
Name:gag
OrganismiMouse mammary tumor virus (strain BR6) (MMTV)
Taxonomic identifieri11758 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeBetaretrovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000228400 Componenti: Genome

Subcellular locationi

Matrix protein p10 :
  • Virion 1 Publication
Capsid protein p27 :
  • Virion 1 Publication
Nucleocapsid protein p14 :
  • Virion 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostSequence analysis
ChainiPRO_00004424652 – 591Gag polyproteinAdd BLAST590
ChainiPRO_00000409202 – 99Matrix protein p10Add BLAST98
ChainiPRO_0000040921100 – 195Phosphorylated protein pp21Add BLAST96
ChainiPRO_0000040922196 – 228Protein p3Add BLAST33
ChainiPRO_0000040923229 – 254Protein p8Add BLAST26
ChainiPRO_0000040924255 – 269Protein nAdd BLAST15
ChainiPRO_0000040925270 – 496Capsid protein p27Add BLAST227
ChainiPRO_0000040926497 – 591Nucleocapsid protein p14Add BLAST95

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by host1 Publication1

Post-translational modificationi

Gag polyprotein: Specific enzymatic cleavages in vivo yield mature proteins.1 Publication
Gag polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei99 – 100Cleavage; by viral protease1 Publication2
Sitei195 – 196Cleavage; by viral protease1 Publication2
Sitei228 – 229Cleavage; by viral protease1 Publication2
Sitei254 – 255Cleavage; by viral protease1 Publication2
Sitei269 – 270Cleavage; by viral protease1 Publication2
Sitei496 – 497Cleavage; by viral protease1 Publication2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Miscellaneous databases

PMAP-CutDBiP10258

Interactioni

Subunit structurei

Matrix protein p10: Homodimer; when myristoylated.1 Publication

Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni5 – 8Combined sources4
Helixi9 – 21Combined sources13
Turni22 – 24Combined sources3
Helixi29 – 42Combined sources14
Helixi45 – 49Combined sources5
Helixi54 – 71Combined sources18
Helixi73 – 75Combined sources3
Helixi80 – 90Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZV5X-ray1.57A/B2-92[»]
5HYBX-ray1.94A/B2-92[»]
5I27X-ray2.05A/B6-92[»]
ProteinModelPortaliP10258
SMRiP10258
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi305 – 308PTAP/PSAP motifCurated4

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Gag-p27 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri525 – 542CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri552 – 569CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di1.10.1200.30, 1 hit
1.10.150.490, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR003322 B_retro_matrix
IPR038124 B_retro_matrix_sf
IPR000721 Gag_p24
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF02337 Gag_p10, 1 hit
PF00607 Gag_p24, 1 hit
PF00098 zf-CCHC, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004265 B_retro_matrix_N, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 2 hits
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Gag polyprotein (identifier: P10258-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVSGSKGQK LFVSVLQRLL SERGLHVKES SAIEFYQFLI KVSPWFPEEG
60 70 80 90 100
GLNLQDWKRV GREMKRYAAE HGTDSIPKQA YPIWLQLREI LTEQSDLVLL
110 120 130 140 150
SAEAKSVTEE ELEEGLTGLL STSSQEKTYG TRGTAYAEID TEVDKLSEHI
160 170 180 190 200
YDEPYEEKEK ADKNEEKDHV RKIKKVVQRK ENSEGKRKEK DSKAFLATDW
210 220 230 240 250
NDDDLSPEDW DDLEEQAAHY HDDDELILPV KRKVVKKKPQ ALRRKPLPPV
260 270 280 290 300
GFAGAMAEAR EKGDLTFTFP VVFMGESDED DTPVWEPLPL KTLKELQSAV
310 320 330 340 350
RTMGPSAPYT LQVVDMVASQ WLTPSDWHQT ARATLSPGDY VLWRTEYEEK
360 370 380 390 400
SKEMVQKAAG KRKGKVSLDM LLGTGQFLSP SSQIKLSKDV LKDVTTNAVL
410 420 430 440 450
AWRAIPPPGV KKTVLAGLKQ GNEESYETFI SRLEEAVYRM MPRGEGSDIL
460 470 480 490 500
IKQLAWENAN SLCQDLIRPI RKTGTIQDYI RACLDASPAV VQGMAYAAAM
510 520 530 540 550
RGQKYSTFVK QTYGGGKGGQ GAEGPVCFSC GKTGHIRKDC KDEKGSKRAP
560 570 580 590
PGLCPRCKKG YHWKSECKSK FDKDGNPLPP LETNAENSKN L
Note: Produced by conventional translation.1 Publication
Length:591
Mass (Da):66,269
Last modified:January 23, 2007 - v3
Checksum:i8A5C2212460864A5
GO
Isoform Gag-Pro polyprotein (identifier: P10271-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P10271.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting between gag-pro.1 Publication
Length:860
Mass (Da):95,409
GO
Isoform Gag-Pro-Pol polyprotein (identifier: P03365-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P03365.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshiftings between gag-pro and pro-pol.1 Publication
Length:1,755
Mass (Da):197,115
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15122 Genomic RNA Translation: AAA46543.1
PIRiA26795 FOMVMM
RefSeqiNP_056882.1, NC_001503.1

Genome annotation databases

GeneIDi1491864
KEGGivg:1491864

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiGAG_MMTVB
AccessioniPrimary (citable) accession number: P10258
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 112 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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