Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P10249

- SUCP_STRMU

UniProt

P10249 - SUCP_STRMU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Sucrose phosphorylase

Gene

gtfA

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Intracellular catabolism of sucrose. Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activityi

Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate.

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. sucrose phosphorylase activity Source: CACAO

GO - Biological processi

  1. sucrose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-846-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose phosphorylase (EC:2.4.1.7)
Alternative name(s):
Glucosyltransferase-A
Short name:
GTF-A
Sucrose glucosyltransferase
Gene namesi
Name:gtfA
Ordered Locus Names:SMU_881
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

Pathology & Biotechi

Keywords - Diseasei

Dental caries

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 481480Sucrose phosphorylasePRO_0000072301Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU.881.

Structurei

3D structure databases

ProteinModelPortaliP10249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sucrose phosphorylase family.Curated

Phylogenomic databases

eggNOGiCOG0366.
KOiK00690.
OMAiFRNNEPA.
OrthoDBiEOG6XM77N.
PhylomeDBiP10249.

Family and domain databases

Gene3Di3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR016377. Sucrose_phosphorylase.
IPR022527. Sucrose_phosphorylase_GftA.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF003059. Sucrose_phosphorylase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR03852. sucrose_gtfA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10249-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIINKTMLI TYADSLGKNL KELNENIENY FGDAVGGVHL LPFFPSTGDR
60 70 80 90 100
GFAPIDYHEV DSAFGDWDDV KCLGEKYYLM FDFMINHISR QSKYYKDYQE
110 120 130 140 150
KHEASAYKDL FLNWDKFWPK NRPTQEDVDL IYKRKDRAPK QEIQFADGSV
160 170 180 190 200
EHLWNTFGEE QIDLDVTKEV TMDFIRSTIE NLAANGCDLI RLDAFAYAVK
210 220 230 240 250
KLDTNDFFVE PEIWTLLDKV RDIAAVSGAE ILPEIHEHYT IQFKIADHDY
260 270 280 290 300
YVYDFALPMV TLYSLYSSKV DRLAKWLKMS PMKQFTTLDT HDGIGVVDVK
310 320 330 340 350
DILTDEEITY TSNELYKVGA NVNRKYSTAE YNNLDIYQIN STYYSALGDD
360 370 380 390 400
DQKYFLARLI QAFAPGIPQV YYVGFLAGKN DLELLESTKE GRNINRHYYS
410 420 430 440 450
SEEIAKEVKR PVVKALLNLF TYRNQSAAFD LDGRIEVETP NEATIVIERQ
460 470 480
NKDGSHIAKA EINLQDMTYR VTENDQTISF E
Length:481
Mass (Da):55,687
Last modified:January 23, 2007 - v4
Checksum:i1B403FD6D95FFC8C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41I → T(PubMed:3194228)Curated
Sequence conflicti4 – 41I → T(PubMed:2967248)Curated
Sequence conflicti5 – 51N → I(PubMed:3194228)Curated
Sequence conflicti32 – 321G → A in CAA30846. (PubMed:3194228)Curated
Sequence conflicti72 – 721C → R(PubMed:3194228)Curated
Sequence conflicti72 – 721C → R(PubMed:2967248)Curated
Sequence conflicti222 – 2221D → N in AAA26937. (PubMed:2967248)Curated
Sequence conflicti268 – 2681S → G(PubMed:3194228)Curated
Sequence conflicti268 – 2681S → G(PubMed:2967248)Curated
Sequence conflicti277 – 2771L → V in CAA30846. (PubMed:3194228)Curated
Sequence conflicti393 – 3931N → I in CAA30846. (PubMed:3194228)Curated
Sequence conflicti424 – 4241N → I in CAA30846. (PubMed:3194228)Curated
Sequence conflicti443 – 4442AT → EN in CAA30846. (PubMed:3194228)Curated
Sequence conflicti459 – 4591K → T(PubMed:3194228)Curated
Sequence conflicti459 – 4591K → T(PubMed:2967248)Curated
Sequence conflicti480 – 4812FE → LSMISCQT in CAA30846. (PubMed:3194228)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08057 Genomic DNA. Translation: CAA30846.1.
M77351 Genomic DNA. Translation: AAA26937.1.
AE014133 Genomic DNA. Translation: AAN58596.1.
PIRiA27626.
S01972. BWSOGM.
RefSeqiNP_721290.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58596; AAN58596; SMU_881.
GeneIDi1028245.
KEGGismu:SMU_881.
PATRICi19663909. VBIStrMut61772_0787.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08057 Genomic DNA. Translation: CAA30846.1 .
M77351 Genomic DNA. Translation: AAA26937.1 .
AE014133 Genomic DNA. Translation: AAN58596.1 .
PIRi A27626.
S01972. BWSOGM.
RefSeqi NP_721290.1. NC_004350.2.

3D structure databases

ProteinModelPortali P10249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 210007.SMU.881.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN58596 ; AAN58596 ; SMU_881 .
GeneIDi 1028245.
KEGGi smu:SMU_881.
PATRICi 19663909. VBIStrMut61772_0787.

Phylogenomic databases

eggNOGi COG0366.
KOi K00690.
OMAi FRNNEPA.
OrthoDBi EOG6XM77N.
PhylomeDBi P10249.

Enzyme and pathway databases

BioCyci SMUT210007:GC7Z-846-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 2 hits.
InterProi IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR016377. Sucrose_phosphorylase.
IPR022527. Sucrose_phosphorylase_GftA.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF003059. Sucrose_phosphorylase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
TIGRFAMsi TIGR03852. sucrose_gtfA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the gtfA gene from S. mutans GS-5."
    James L.C., Hughes T.A., Curtiss R. III
    Nucleic Acids Res. 16:10398-10398(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GS-5.
  2. "Sequence analysis of the glucosyltransferase A gene (gtfA) from Streptococcus mutans Ingbritt."
    Ferretti J.J., Huang T.-T., Russell R.R.B.
    Infect. Immun. 56:1585-1588(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
    Strain: Ingbritt.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.
  4. "Streptococcus mutans gtfA gene specifies sucrose phosphorylase."
    Russell R.R.B., Mukasa H., Shimamura A., Ferretti J.J.
    Infect. Immun. 56:2763-2765(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiSUCP_STRMU
AccessioniPrimary (citable) accession number: P10249
Secondary accession number(s): Q99064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 115 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Some earlier publications concluded that the product of the reaction of GtfA with sucrose was a glucan, because of its insolubility in ethanol.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3