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P10249

- SUCP_STRMU

UniProt

P10249 - SUCP_STRMU

Protein

Sucrose phosphorylase

Gene

gtfA

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Intracellular catabolism of sucrose. Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

    Catalytic activityi

    Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate.

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. sucrose phosphorylase activity Source: CACAO

    GO - Biological processi

    1. sucrose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSMUT210007:GC7Z-846-MONOMER.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sucrose phosphorylase (EC:2.4.1.7)
    Alternative name(s):
    Glucosyltransferase-A
    Short name:
    GTF-A
    Sucrose glucosyltransferase
    Gene namesi
    Name:gtfA
    Ordered Locus Names:SMU_881
    OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
    Taxonomic identifieri210007 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002512: Chromosome

    Pathology & Biotechi

    Keywords - Diseasei

    Dental caries

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 481480Sucrose phosphorylasePRO_0000072301Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi210007.SMU.881.

    Structurei

    3D structure databases

    ProteinModelPortaliP10249.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the sucrose phosphorylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0366.
    KOiK00690.
    OMAiFRNNEPA.
    OrthoDBiEOG6XM77N.
    PhylomeDBiP10249.

    Family and domain databases

    Gene3Di3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR016377. Sucrose_phosphorylase.
    IPR022527. Sucrose_phosphorylase_GftA.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003059. Sucrose_phosphorylase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03852. sucrose_gtfA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10249-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIINKTMLI TYADSLGKNL KELNENIENY FGDAVGGVHL LPFFPSTGDR    50
    GFAPIDYHEV DSAFGDWDDV KCLGEKYYLM FDFMINHISR QSKYYKDYQE 100
    KHEASAYKDL FLNWDKFWPK NRPTQEDVDL IYKRKDRAPK QEIQFADGSV 150
    EHLWNTFGEE QIDLDVTKEV TMDFIRSTIE NLAANGCDLI RLDAFAYAVK 200
    KLDTNDFFVE PEIWTLLDKV RDIAAVSGAE ILPEIHEHYT IQFKIADHDY 250
    YVYDFALPMV TLYSLYSSKV DRLAKWLKMS PMKQFTTLDT HDGIGVVDVK 300
    DILTDEEITY TSNELYKVGA NVNRKYSTAE YNNLDIYQIN STYYSALGDD 350
    DQKYFLARLI QAFAPGIPQV YYVGFLAGKN DLELLESTKE GRNINRHYYS 400
    SEEIAKEVKR PVVKALLNLF TYRNQSAAFD LDGRIEVETP NEATIVIERQ 450
    NKDGSHIAKA EINLQDMTYR VTENDQTISF E 481
    Length:481
    Mass (Da):55,687
    Last modified:January 23, 2007 - v4
    Checksum:i1B403FD6D95FFC8C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41I → T(PubMed:3194228)Curated
    Sequence conflicti4 – 41I → T(PubMed:2967248)Curated
    Sequence conflicti5 – 51N → I(PubMed:3194228)Curated
    Sequence conflicti32 – 321G → A in CAA30846. (PubMed:3194228)Curated
    Sequence conflicti72 – 721C → R(PubMed:3194228)Curated
    Sequence conflicti72 – 721C → R(PubMed:2967248)Curated
    Sequence conflicti222 – 2221D → N in AAA26937. (PubMed:2967248)Curated
    Sequence conflicti268 – 2681S → G(PubMed:3194228)Curated
    Sequence conflicti268 – 2681S → G(PubMed:2967248)Curated
    Sequence conflicti277 – 2771L → V in CAA30846. (PubMed:3194228)Curated
    Sequence conflicti393 – 3931N → I in CAA30846. (PubMed:3194228)Curated
    Sequence conflicti424 – 4241N → I in CAA30846. (PubMed:3194228)Curated
    Sequence conflicti443 – 4442AT → EN in CAA30846. (PubMed:3194228)Curated
    Sequence conflicti459 – 4591K → T(PubMed:3194228)Curated
    Sequence conflicti459 – 4591K → T(PubMed:2967248)Curated
    Sequence conflicti480 – 4812FE → LSMISCQT in CAA30846. (PubMed:3194228)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X08057 Genomic DNA. Translation: CAA30846.1.
    M77351 Genomic DNA. Translation: AAA26937.1.
    AE014133 Genomic DNA. Translation: AAN58596.1.
    PIRiA27626.
    S01972. BWSOGM.
    RefSeqiNP_721290.1. NC_004350.2.

    Genome annotation databases

    EnsemblBacteriaiAAN58596; AAN58596; SMU_881.
    GeneIDi1028245.
    KEGGismu:SMU_881.
    PATRICi19663909. VBIStrMut61772_0787.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X08057 Genomic DNA. Translation: CAA30846.1 .
    M77351 Genomic DNA. Translation: AAA26937.1 .
    AE014133 Genomic DNA. Translation: AAN58596.1 .
    PIRi A27626.
    S01972. BWSOGM.
    RefSeqi NP_721290.1. NC_004350.2.

    3D structure databases

    ProteinModelPortali P10249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 210007.SMU.881.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN58596 ; AAN58596 ; SMU_881 .
    GeneIDi 1028245.
    KEGGi smu:SMU_881.
    PATRICi 19663909. VBIStrMut61772_0787.

    Phylogenomic databases

    eggNOGi COG0366.
    KOi K00690.
    OMAi FRNNEPA.
    OrthoDBi EOG6XM77N.
    PhylomeDBi P10249.

    Enzyme and pathway databases

    BioCyci SMUT210007:GC7Z-846-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR016377. Sucrose_phosphorylase.
    IPR022527. Sucrose_phosphorylase_GftA.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003059. Sucrose_phosphorylase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR03852. sucrose_gtfA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gtfA gene from S. mutans GS-5."
      James L.C., Hughes T.A., Curtiss R. III
      Nucleic Acids Res. 16:10398-10398(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GS-5.
    2. "Sequence analysis of the glucosyltransferase A gene (gtfA) from Streptococcus mutans Ingbritt."
      Ferretti J.J., Huang T.-T., Russell R.R.B.
      Infect. Immun. 56:1585-1588(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
      Strain: Ingbritt.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700610 / UA159.
    4. "Streptococcus mutans gtfA gene specifies sucrose phosphorylase."
      Russell R.R.B., Mukasa H., Shimamura A., Ferretti J.J.
      Infect. Immun. 56:2763-2765(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiSUCP_STRMU
    AccessioniPrimary (citable) accession number: P10249
    Secondary accession number(s): Q99064
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Some earlier publications concluded that the product of the reaction of GtfA with sucrose was a glucan, because of its insolubility in ethanol.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3