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Reviewed, UniProtKB/Swiss-Prot P10242 (MYB_HUMAN)

Last modified November 25, 2008. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Myb proto-oncogene protein
Alternative name(s):
    C-myb
Gene names
Name: MYB
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

Subunit structure

Binds MYBBP1A. Interacts with HIPK2 and NLK By similarity.

Subcellular location

Nucleus.

Domain

Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.

Post-translational modification

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation Probable.

Phosphorylated by NLK on multiple sites, which induces proteasomal degradation By similarity.

Sequence similarities

Contains 3 HTH myb-type DNA-binding domains.

Ontologies

Keywords

   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DiseaseProto-oncogene
   DomainRepeat
   LigandDNA-binding
   Molecular functionActivator
   PTMUbl conjugation

Gene Ontology (GO)

   Biological processregulation of transcription, DNA-dependent Ref.10

Non-traceable author statement. Source: UniProtKB

   Cellular componentnuclear matrix Ref.9

Non-traceable author statement. Source: UniProtKB

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction. Source: IntAct

transcription activator activity Ref.10

Non-traceable author statement. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CrebbpP454811EBI-298355,EBI-296306From a different organism.
HIPK2Q9H2X61EBI-298355,EBI-348345
MED15Q96RN51EBI-298355,EBI-394506
NLKQ9UBE81EBI-298355,EBI-366978

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P10242-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P10242-2)

The sequence of this isoform differs from the canonical sequence as follows:
     314-316: Missing.
Isoform 3 (identifier: P10242-3)

The sequence of this isoform differs from the canonical sequence as follows:
     317-350: TQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGE → LCGPLLNSDIFSDWAANWDGSLCFATYIVNQQRQ
     351-640: Missing.
Isoform 4 (identifier: P10242-4)

The sequence of this isoform differs from the canonical sequence as follows:
     401-401: S → SDSSSWCDLS...VKSLPFSPSQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Sequence conflict4331L → F in CAA35503. Ref.5
Isoform 5 (identifier: P10242-5)

The sequence of this isoform differs from the canonical sequence as follows:
     402-402: F → M
     403-640: Missing.
Isoform 6 (identifier: P10242-6)

The sequence of this isoform differs from the canonical sequence as follows:
     567-640: NILTSSVLMA...NAFSARTLVM → TGVQWHDFGS...LRFNGTSIRR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640Myb proto-oncogene protein
PRO_0000197048

Regions

Domain35 – 8652HTH myb-type 1
Domain87 – 14256HTH myb-type 2
Domain143 – 19351HTH myb-type 3
Domain376 – 39722Leucine-zipper
DNA binding63 – 8624H-T-H motif By similarity
DNA binding115 – 13824H-T-H motif By similarity
DNA binding166 – 18924H-T-H motif By similarity
Region90 – 193104Interaction with HIPK2 and NLK By similarity
Region275 – 32753Transcriptional activation domain
Region328 – 465138Negative regulatory domain By similarity

Natural variations

Alternative sequence314 – 3163Missing in isoform 2.
VSP_003293
Alternative sequence317 – 35034TQNHT…SCLGE → LCGPLLNSDIFSDWAANWDG SLCFATYIVNQQRQ in isoform 3.
VSP_003294
Alternative sequence351 – 640290Missing in isoform 3.
VSP_003295
Alternative sequence4011S → SDSSSWCDLSSFEFFEEADF SPSQHHTGKALQLQQREGNG TKPAGEPSPRVNKRMLSESS LDPPKVLPPARHSTIPLVIL RKKRGQASPLATGDCSSFIF ADVSSSTPKRSPVKSLPFSP SQ in isoform 4.
VSP_003296
Alternative sequence4021F → M in isoform 5.
VSP_003297
Alternative sequence403 – 640238Missing in isoform 5.
VSP_003298
Alternative sequence567 – 64074NILTS…RTLVM → TGVQWHDFGSLQPLPPGFKR FSCLSLPRSWDYRHPPPRPA NFEFLVETGFLHVGQAGLEL LTSGDLPASASQSARITGVS HRARPEYSYKLRFNGTSIRR in isoform 6.
VSP_003299

Experimental info

Sequence conflict5111I → F in AAA52032. Ref.1
Sequence conflict5631A → R in AAA52032. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: FD5E6694020426ED

FASTA64072,341
        10         20         30         40         50         60 
MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT 

        70         80         90        100        110        120 
DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK 

       130        140        150        160        170        180 
HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA 

       190        200        210        220        230        240 
IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV 

       250        260        270        280        290        300 
NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL 

       310        320        330        340        350        360 
LLMSTENELK GQQVLPTQNH TCSYPGWHST TIADHTRPHG DSAPVSCLGE HHSTPSLPAD 

       370        380        390        400        410        420 
PGSLPEESAS PARCMIVHQG TILDNVKNLL EFAETLQFID SFLNTSSNHE NSDLEMPSLT 

       430        440        450        460        470        480 
STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK 

       490        500        510        520        530        540 
YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF 

       550        560        570        580        590        600 
FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP 

       610        620        630        640 
LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM 

« Hide

Isoform 2 [UniParc].

Checksum: 75D8144FEEC381C5
Show »

63772,032
Isoform 3 [UniParc].

Checksum: 60632631CFEE51FF
Show »

35040,620
Isoform 4 [UniParc].

Checksum: 9D11661393B1A85B
Show »

76185,475
Isoform 5 [UniParc].

Checksum: 331FC406EC1C335C
Show »

40246,065
Isoform 6 [UniParc].

Checksum: BF91C8F858EA12DF
Show »

66675,526

References

« Hide 'large scale' references
[1]"Human c-myb protooncogene: nucleotide sequence of cDNA and organization of the genomic locus."
Majello B., Kenyon L.C., Dalla-Favera R.
Proc. Natl. Acad. Sci. U.S.A. 83:9636-9640(1986) [PubMed: 3540945] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Alternative splicing of the human c-myb gene."
Westin E.H., Gorse K.M., Clarke M.F.
Oncogene 5:1117-1124(1990) [PubMed: 2202948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Characterization of the complete sequence structure of the human c-myb gene."
Westin E.H., Gorse K.M.
Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 3; 4; 5 AND 6).
Tissue: Liver and Placenta.
[4]Gaillard C., Perbal B.
Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]"Identification of alternatively spliced transcripts for human c-myb: molecular cloning and sequence analysis of human c-myb exon 9A sequences."
Dasgupta P., Reddy E.P.
Oncogene 4:1419-1423(1989) [PubMed: 2687764] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[7]"Identification of a second promoter in the human c-myb proto-oncogene."
Jacobs S.M., Gorse K.M., Westin E.H.
Oncogene 9:227-235(1994) [PubMed: 8302584] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-47.
[8]"Positive autoregulation of c-myb expression via Myb binding sites in the 5' flanking region of the human c-myb gene."
Nicolaides N.C., Gualdi R., Casadevall C., Manzella L., Calabretta B.
Mol. Cell. Biol. 11:6166-6176(1991) [PubMed: 1944282] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
[9]"Studies of the human c-myb gene and its product in human acute leukemias."
Slamon D.J., Boone T.C., Murdock D.C., Keith D.E., Press M.F., Larson R.A., Souza L.M.
Science 233:347-351(1986) [PubMed: 3014652] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-640 (ISOFORM 1).
[10]"Transcriptional activation by human c-myb and v-myb genes."
Kalkbrenner F., Guehmann S., Moelling K.
Oncogene 5:657-661(1990) [PubMed: 2189102] [Abstract]
Cited for: TRANSCRIPTION ACTIVATION DOMAIN.
[11]"p53 suppresses the c-Myb-induced activation of heat shock transcription factor 3."
Tanikawa J., Ichikawa-Iwata E., Kanei-Ishii C., Nakai A., Matsuzawa S., Reed J.C., Ishii S.
J. Biol. Chem. 275:15578-15585(2000) [PubMed: 10747903] [Abstract]
Cited for: INTERACTION WITH SIAH1, DEGRADATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

M15024 mRNA. Translation: AAA52032.1.
X52125 mRNA. Translation: CAA36371.1.
U22376 Genomic DNA. Translation: AAB49034.1.
U22376 Genomic DNA. Translation: AAB49035.1.
U22376 Genomic DNA. Translation: AAB49036.1.
U22376 Genomic DNA. Translation: AAB49037.1.
U22376 Genomic DNA. Translation: AAB49038.1.
U22376 Genomic DNA. Translation: AAB49039.1.
AF104863 mRNA. Translation: AAC96326.1.
X17469 mRNA. Translation: CAA35503.1.
BC064955 mRNA. Translation: AAH64955.1.
M13665 mRNA. Translation: AAA52030.1.
M13666 mRNA. Translation: AAA52031.1. Sequence problems.
PIRTVHUMB. A26661.
RefSeqNP_005366.2.
UniGeneHs.654446

3D structure databases

HSSPHSSP built from PDB template 1MBE based on UniProtKB P06876.
SMRP10242. Positions 39-190.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1057N.
IntActP10242.

PTM databases

PhosphoSiteP10242.

Genome annotation databases

EnsemblENSG00000118513. Homo sapiens. [Contig view]
GeneID4602.
KEGGhsa:4602.

Organism-specific databases

H-InvDBHIX0032777.
HGNCHGNC:7545. MYB.
HPACAB017704.
MIM189990. gene.
PharmGKBPA31345.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOVERGENP10242.

Gene expression databases

ArrayExpressP10242.
CleanExHS_MYB.
GermOnlineENSG00000118513. Homo sapiens.

Family and domain databases

InterProIPR015395. C-myb_C.
IPR012287. Homeodomain-rel.
IPR014778. Myb_DNA-bd.
IPR015495. Myb_trans_fac.
IPR001005. SANT_DNA-bd.
IPR012642. Wos2.
[Graphical view]
Gene3DG3DSA:1.10.10.60. Homeodomain-rel. 3 hits.
PANTHERPTHR10641. Myb_transfac. 1 hit.
PfamPF09316. Cmyb_C. 1 hit.
PF00249. Myb_DNA-binding. 3 hits.
PF07988. Wos2. 1 hit.
[Graphical view]
SMARTSM00717. SANT. 3 hits.
[Graphical view]
PROSITEPS51294. HTH_MYB. 3 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP10242.
NextBio17700.
SOURCESearch...

Entry information

Entry nameMYB_HUMAN
AccessionPrimary (citable) accession number: P10242
Secondary accession number(s): P78391 expand/collapse secondary AC list , P78392, P78525, P78526, Q14023, Q14024, Q9UE83
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 25, 2008
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents