Myb proto-oncogene protein - Homo sapiens (Human)

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Names and origin

Protein names

Recommended name:
Myb proto-oncogene protein
Alternative name(s):
C-myb

Gene names

Name:

MYB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Subunit structure	Binds MYBBP1A. Interacts with HIPK2 and NLK By similarity.
Subcellular location	Nucleus.
Domain	Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
Post-translational modification	Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation Probable. Phosphorylated by NLK on multiple sites, which induces proteasomal degradation By similarity.
Sequence similarities	Contains 3 HTH myb-type DNA-binding domains.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Disease	Proto-oncogene
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator
PTM	Ubl conjugation
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Ref.10 Non-traceable author statement. Source: UniProtKB
Cellular component	nuclear matrix Ref.9 Non-traceable author statement. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct transcription activator activity Ref.10 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Crebbp	P45481	1	EBI-298355,EBI-296306	From a different organism.
HIPK2	Q9H2X6	1	EBI-298355,EBI-348345
MED15	Q96RN5	1	EBI-298355,EBI-394506
NLK	Q9UBE8	1	EBI-298355,EBI-366978

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Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P10242-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P10242-2)

The sequence of this isoform differs from the canonical sequence as follows:
314-316: Missing.

Isoform 3 (identifier: P10242-3)

The sequence of this isoform differs from the canonical sequence as follows:
317-350: TQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGE → LCGPLLNSDIFSDWAANWDGSLCFATYIVNQQRQ
351-640: Missing.

Isoform 4 (identifier: P10242-4)

The sequence of this isoform differs from the canonical sequence as follows:
401-401: S → SDSSSWCDLS...VKSLPFSPSQ

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sequence conflict

433

1

L → F in CAA35503. Ref.5

Isoform 5 (identifier: P10242-5)

The sequence of this isoform differs from the canonical sequence as follows:
402-402: F → M
403-640: Missing.

Isoform 6 (identifier: P10242-6)

The sequence of this isoform differs from the canonical sequence as follows:
567-640: NILTSSVLMA...NAFSARTLVM → TGVQWHDFGS...LRFNGTSIRR

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 640

640

Myb proto-oncogene protein

PRO_0000197048

Regions

Domain

35 – 86

52

HTH myb-type 1

Domain

87 – 142

56

HTH myb-type 2

Domain

143 – 193

51

HTH myb-type 3

Domain

376 – 397

22

Leucine-zipper

DNA binding

63 – 86

24

H-T-H motif By similarity

DNA binding

115 – 138

24

H-T-H motif By similarity

DNA binding

166 – 189

24

H-T-H motif By similarity

Region

90 – 193

104

Interaction with HIPK2 and NLK By similarity

Region

275 – 327

53

Transcriptional activation domain

Region

328 – 465

138

Negative regulatory domain By similarity

Natural variations

Alternative sequence

314 – 316

3

Missing in isoform 2.

VSP_003293

Alternative sequence

317 – 350

34

TQNHT…SCLGE → LCGPLLNSDIFSDWAANWDG SLCFATYIVNQQRQ in isoform 3.

VSP_003294

Alternative sequence

351 – 640

290

Missing in isoform 3.

VSP_003295

Alternative sequence

401

1

S → SDSSSWCDLSSFEFFEEADF SPSQHHTGKALQLQQREGNG TKPAGEPSPRVNKRMLSESS LDPPKVLPPARHSTIPLVIL RKKRGQASPLATGDCSSFIF ADVSSSTPKRSPVKSLPFSP SQ in isoform 4.

VSP_003296

Alternative sequence

402

1

F → M in isoform 5.

VSP_003297

Alternative sequence

403 – 640

238

Missing in isoform 5.

VSP_003298

Alternative sequence

567 – 640

74

NILTS…RTLVM → TGVQWHDFGSLQPLPPGFKR FSCLSLPRSWDYRHPPPRPA NFEFLVETGFLHVGQAGLEL LTSGDLPASASQSARITGVS HRARPEYSYKLRFNGTSIRR in isoform 6.

VSP_003299

Experimental info

Sequence conflict

511

1

I → F in AAA52032. Ref.1

Sequence conflict

563

1

A → R in AAA52032. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: FD5E6694020426ED Show »	FASTA	640	72,341
10 20 30 40 50 60 MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT 70 80 90 100 110 120 DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK 130 140 150 160 170 180 HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA 190 200 210 220 230 240 IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV 250 260 270 280 290 300 NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL 310 320 330 340 350 360 LLMSTENELK GQQVLPTQNH TCSYPGWHST TIADHTRPHG DSAPVSCLGE HHSTPSLPAD 370 380 390 400 410 420 PGSLPEESAS PARCMIVHQG TILDNVKNLL EFAETLQFID SFLNTSSNHE NSDLEMPSLT 430 440 450 460 470 480 STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK 490 500 510 520 530 540 YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF 550 560 570 580 590 600 FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP 610 620 630 640 LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM « Hide
Isoform 2 [UniParc]. Checksum: 75D8144FEEC381C5 Show »		637	72,032
10 20 30 40 50 60 MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT 70 80 90 100 110 120 DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK 130 140 150 160 170 180 HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA 190 200 210 220 230 240 IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV 250 260 270 280 290 300 NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL 310 320 330 340 350 360 LLMSTENELK GQQTQNHTCS YPGWHSTTIA DHTRPHGDSA PVSCLGEHHS TPSLPADPGS 370 380 390 400 410 420 LPEESASPAR CMIVHQGTIL DNVKNLLEFA ETLQFIDSFL NTSSNHENSD LEMPSLTSTP 430 440 450 460 470 480 LIGHKLTVTT PFHRDQTVKT QKENTVFRTP AIKRSILESS PRTPTPFKHA LAAQEIKYGP 490 500 510 520 530 540 LKMLPQTPSH LVEDLQDVIK QESDESGIVA EFQENGPPLL KKIKQEVESP TDKSGNFFCS 550 560 570 580 590 600 HHWEGDSLNT QLFTQTSPVA DAPNILTSSV LMAPASEDED NVLKAFTVPK NRSLASPLQP 610 620 630 CSSTWEPASC GKMEEQMTSS SQARKYVNAF SARTLVM « Hide
Isoform 3 [UniParc]. Checksum: 60632631CFEE51FF Show »		350	40,620
10 20 30 40 50 60 MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT 70 80 90 100 110 120 DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK 130 140 150 160 170 180 HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA 190 200 210 220 230 240 IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV 250 260 270 280 290 300 NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL 310 320 330 340 350 LLMSTENELK GQQVLPLCGP LLNSDIFSDW AANWDGSLCF ATYIVNQQRQ « Hide
Isoform 4 [UniParc]. Checksum: 9D11661393B1A85B Show »		761	85,475
10 20 30 40 50 60 MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT 70 80 90 100 110 120 DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK 130 140 150 160 170 180 HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA 190 200 210 220 230 240 IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV 250 260 270 280 290 300 NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL 310 320 330 340 350 360 LLMSTENELK GQQVLPTQNH TCSYPGWHST TIADHTRPHG DSAPVSCLGE HHSTPSLPAD 370 380 390 400 410 420 PGSLPEESAS PARCMIVHQG TILDNVKNLL EFAETLQFID SDSSSWCDLS SFEFFEEADF 430 440 450 460 470 480 SPSQHHTGKA LQLQQREGNG TKPAGEPSPR VNKRMLSESS LDPPKVLPPA RHSTIPLVIL 490 500 510 520 530 540 RKKRGQASPL ATGDCSSFIF ADVSSSTPKR SPVKSLPFSP SQFLNTSSNH ENSDLEMPSL 550 560 570 580 590 600 TSTPLIGHKL TVTTPFHRDQ TVKTQKENTV FRTPAIKRSI LESSPRTPTP FKHALAAQEI 610 620 630 640 650 660 KYGPLKMLPQ TPSHLVEDLQ DVIKQESDES GIVAEFQENG PPLLKKIKQE VESPTDKSGN 670 680 690 700 710 720 FFCSHHWEGD SLNTQLFTQT SPVADAPNIL TSSVLMAPAS EDEDNVLKAF TVPKNRSLAS 730 740 750 760 PLQPCSSTWE PASCGKMEEQ MTSSSQARKY VNAFSARTLV M « Hide
Isoform 5 [UniParc]. Checksum: 331FC406EC1C335C Show »		402	46,065
10 20 30 40 50 60 MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT 70 80 90 100 110 120 DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK 130 140 150 160 170 180 HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA 190 200 210 220 230 240 IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV 250 260 270 280 290 300 NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL 310 320 330 340 350 360 LLMSTENELK GQQVLPTQNH TCSYPGWHST TIADHTRPHG DSAPVSCLGE HHSTPSLPAD 370 380 390 400 PGSLPEESAS PARCMIVHQG TILDNVKNLL EFAETLQFID SM « Hide
Isoform 6 [UniParc]. Checksum: BF91C8F858EA12DF Show »		666	75,526
10 20 30 40 50 60 MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT 70 80 90 100 110 120 DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK 130 140 150 160 170 180 HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA 190 200 210 220 230 240 IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV 250 260 270 280 290 300 NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL 310 320 330 340 350 360 LLMSTENELK GQQVLPTQNH TCSYPGWHST TIADHTRPHG DSAPVSCLGE HHSTPSLPAD 370 380 390 400 410 420 PGSLPEESAS PARCMIVHQG TILDNVKNLL EFAETLQFID SFLNTSSNHE NSDLEMPSLT 430 440 450 460 470 480 STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK 490 500 510 520 530 540 YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF 550 560 570 580 590 600 FCSHHWEGDS LNTQLFTQTS PVADAPTGVQ WHDFGSLQPL PPGFKRFSCL SLPRSWDYRH 610 620 630 640 650 660 PPPRPANFEF LVETGFLHVG QAGLELLTSG DLPASASQSA RITGVSHRAR PEYSYKLRFN GTSIRR « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human c-myb protooncogene: nucleotide sequence of cDNA and organization of the genomic locus." Majello B., Kenyon L.C., Dalla-Favera R. Proc. Natl. Acad. Sci. U.S.A. 83:9636-9640(1986) [PubMed: 3540945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Alternative splicing of the human c-myb gene." Westin E.H., Gorse K.M., Clarke M.F. Oncogene 5:1117-1124(1990) [PubMed: 2202948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Characterization of the complete sequence structure of the human c-myb gene." Westin E.H., Gorse K.M. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 3; 4; 5 AND 6). Tissue: Liver and Placenta.
[4]	Gaillard C., Perbal B. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"Identification of alternatively spliced transcripts for human c-myb: molecular cloning and sequence analysis of human c-myb exon 9A sequences." Dasgupta P., Reddy E.P. Oncogene 4:1419-1423(1989) [PubMed: 2687764] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[7]	"Identification of a second promoter in the human c-myb proto-oncogene." Jacobs S.M., Gorse K.M., Westin E.H. Oncogene 9:227-235(1994) [PubMed: 8302584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-47.
[8]	"Positive autoregulation of c-myb expression via Myb binding sites in the 5' flanking region of the human c-myb gene." Nicolaides N.C., Gualdi R., Casadevall C., Manzella L., Calabretta B. Mol. Cell. Biol. 11:6166-6176(1991) [PubMed: 1944282] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
[9]	"Studies of the human c-myb gene and its product in human acute leukemias." Slamon D.J., Boone T.C., Murdock D.C., Keith D.E., Press M.F., Larson R.A., Souza L.M. Science 233:347-351(1986) [PubMed: 3014652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-640 (ISOFORM 1).
[10]	"Transcriptional activation by human c-myb and v-myb genes." Kalkbrenner F., Guehmann S., Moelling K. Oncogene 5:657-661(1990) [PubMed: 2189102] [Abstract] Cited for: TRANSCRIPTION ACTIVATION DOMAIN.
[11]	"p53 suppresses the c-Myb-induced activation of heat shock transcription factor 3." Tanikawa J., Ichikawa-Iwata E., Kanei-Ishii C., Nakai A., Matsuzawa S., Reed J.C., Ishii S. J. Biol. Chem. 275:15578-15585(2000) [PubMed: 10747903] [Abstract] Cited for: INTERACTION WITH SIAH1, DEGRADATION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	M15024 mRNA. Translation: AAA52032.1. X52125 mRNA. Translation: CAA36371.1. U22376 Genomic DNA. Translation: AAB49034.1. U22376 Genomic DNA. Translation: AAB49035.1. U22376 Genomic DNA. Translation: AAB49036.1. U22376 Genomic DNA. Translation: AAB49037.1. U22376 Genomic DNA. Translation: AAB49038.1. U22376 Genomic DNA. Translation: AAB49039.1. AF104863 mRNA. Translation: AAC96326.1. X17469 mRNA. Translation: CAA35503.1. BC064955 mRNA. Translation: AAH64955.1. M13665 mRNA. Translation: AAA52030.1. M13666 mRNA. Translation: AAA52031.1. Sequence problems.
PIR	TVHUMB. A26661.
RefSeq	NP_005366.2.
UniGene	Hs.654446
3D structure databases
HSSP	HSSP built from PDB template 1MBE based on UniProtKB P06876.
SMR	P10242. Positions 39-190.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1057N.
IntAct	P10242.
PTM databases
PhosphoSite	P10242.
Genome annotation databases
Ensembl	ENSG00000118513. Homo sapiens. [Contig view]
GeneID	4602.
KEGG	hsa:4602.
Organism-specific databases
H-InvDB	HIX0032777.
HGNC	HGNC:7545. MYB.
HPA	CAB017704.
MIM	189990. gene.
PharmGKB	PA31345.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P10242.
Gene expression databases
ArrayExpress	P10242.
CleanEx	HS_MYB.
GermOnline	ENSG00000118513. Homo sapiens.
Family and domain databases
InterPro	IPR015395. C-myb_C. IPR012287. Homeodomain-rel. IPR014778. Myb_DNA-bd. IPR015495. Myb_trans_fac. IPR001005. SANT_DNA-bd. IPR012642. Wos2. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 3 hits.
PANTHER	PTHR10641. Myb_transfac. 1 hit.
Pfam	PF09316. Cmyb_C. 1 hit. PF00249. Myb_DNA-binding. 3 hits. PF07988. Wos2. 1 hit. [Graphical view]
SMART	SM00717. SANT. 3 hits. [Graphical view]
PROSITE	PS51294. HTH_MYB. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P10242.
NextBio	17700.
SOURCE	Search...

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Entry information

Entry name

MYB_HUMAN

Accession

Primary (citable) accession number: P10242
Secondary accession number(s): P78391

Q9UE83

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents