P10236 (PRIM_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase
EC=2.7.7.-

Gene names

ORF Names:

UL52

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1058 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The UL52 subunit bears the primase activity. Ref.5
Subunit structure	Associates with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor.
Subcellular location	Host nucleus By similarity.
Sequence similarities	Belongs to the herpesviridae UL52 family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Host nucleus
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: GOC
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA primase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1058	1058	DNA primase		PRO_0000116104
Regions
	Compositional bias	177 – 180	4	Poly-Ala
	Compositional bias	310 – 313	4	Poly-Ala
Natural variations
	Natural variant	211	1	V → A in strain: Nonneuroinvasive mutant HF10.
	Natural variant	364	1	S → N in strain: Nonneuroinvasive mutant HF10.
	Natural variant	515	1	P → T in strain: Nonneuroinvasive mutant HF10.

Sequences

	Sequence		Length	Mass (Da)
	P10236 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: CB39341C31B768B4	FASTA	1,058	114,424
	10 20 30 40 50 60 MGQEDGNRGE RRAAGTPVEV TALYATDGCV ITSSIALLTN SLLGAEPVYI FSYDAYTHDG 70 80 90 100 110 120 RADGPTEQDR FEESRALYQA SGGLNGDSFR VTFCLLGTEV GGTHQARGRT RPMFVCRFER 130 140 150 160 170 180 ADDVAALQDA LAHGTPLQPD HIAATLDAEA TFALHANMIL ALTVAINNAS PRTGRDAAAA 190 200 210 220 230 240 QYDQGASLRS LVGRTSLGQR GLTTLYVHHE VRVLAAYRRA YYGSAQSPFW FLSKFGPDEK 250 260 270 280 290 300 SLVLTTRYYL LQAQRLGGAG ATYDLQAIKD ICATYAIPHA PRPDTVSAAS LTSFAAITRF 310 320 330 340 350 360 CCTSQYARGA AAAGFPLYVE RRIAADVRET SALEKFITHD RSCLRVSDRE FITYIYLAHF 370 380 390 400 410 420 ECFSPPRLAT HLRAVTTHDP NPAASTEQPS PLGREAVEQF FCHVRAQLNI GEYVKHNVTP 430 440 450 460 470 480 RETVLDGDTA KAYLRARTYA PGALTPAPAY CGAVDSATKM MGRLADAEKL LVPRGWPAFA 490 500 510 520 530 540 PASPGEDTAG GTPPPQTCGI VKRLLRLAAT EQQGPTPPAI AALIRNAAVQ TPLPVYRISM 550 560 570 580 590 600 VPTGQAFAAL AWDDWARITR DARLAEAVVS AEAAAHPDHG ALGRRLTDRI RAQGPVMPPG 610 620 630 640 650 660 GLDAGGQMYV NRNEIFNGAL AITNIILDLD IALKEPVPFR RLHEALGHFR RGALAAVQLL 670 680 690 700 710 720 FPAARVDPDA YPCYFFKSAC RPGPASVGSG SGLGNDDDGD WFPCYDDAGD EEWAEDPGAM 730 740 750 760 770 780 DTSHDPPDDE VAYFDLCHEV GPTAEPRETD SPVCSCTDKI GLRVCMPVPA PYVVHGSLTM 790 800 810 820 830 840 RGVARVIQQA VLLDRDFVEA IGSYVKNFLL IDTGVYAHGH SLRLPYFAKI APDGPACGRL 850 860 870 880 890 900 LPVFVIPPAC KDVPAFVAAH ADPRRFHFHA PPTYLASPRE IRVLHSLGGD YVSFFERKAS 910 920 930 940 950 960 RNALEHFGRR ETLTEVLGRY NVQPDAGGTV EGFASELLGR IVACIETHFP EHAGEYQAVS 970 980 990 1000 1010 1020 VRRAVSKDDW VLLQLVPVRG TLQQSLSCLR FKHGRASRAT ARTFVALSVG ANNRLCVSLC 1030 1040 1050 QQCFAAKCDS NRLHTLFTID AGTPCSPSVP CSTSQPSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Structures of herpes simplex virus type 1 genes required for replication of virus DNA." McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D. J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus." Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y. Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nonneuroinvasive mutant HF10.
[4]	"Herpes simplex virus type 1 bacterial artificial chromosome." Cunningham C., Davison A.J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 17 syn+.
[5]	"Helicase-primase complex of herpes simplex virus type 1: a mutation in the UL52 subunit abolishes primase activity." Klinedinst D.K., Challberg M.D. J. Virol. 68:3693-3701(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32288.1. M19122 Genomic DNA. Translation: AAA45826.1. DQ889502 Genomic DNA. Translation: ABI63513.1. FJ593289 Genomic DNA. Translation: ACM62276.1.
PIR	WMBE52. E29890.
RefSeq	NP_044655.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1092N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703423.
Phylogenomic databases
ProtClustDB	PHA3145.
Family and domain databases
InterPro	IPR004340. DNA_primase_UL52/UL70_Herpvir. [Graphical view]
Pfam	PF03121. Herpes_UL52. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P10236.
ChEMBL	CHEMBL4380.

Entry information

Entry name

PRIM_HHV11

Accession

Primary (citable) accession number: P10236
Secondary accession number(s): B9VQI1, Q09I82

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program