Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P10236 (PRIM_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA primase

EC=2.7.7.-
Gene names
ORF Names:UL52
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length1058 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The UL52 subunit bears the primase activity. Ref.5

Subunit structure

Associates with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor.

Subcellular location

Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae UL52 family.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentHost nucleus
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA primase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10581058DNA primase
PRO_0000116104

Regions

Compositional bias177 – 1804Poly-Ala
Compositional bias310 – 3134Poly-Ala

Natural variations

Natural variant2111V → A in strain: Nonneuroinvasive mutant HF10.
Natural variant3641S → N in strain: Nonneuroinvasive mutant HF10.
Natural variant5151P → T in strain: Nonneuroinvasive mutant HF10.

Sequences

Sequence LengthMass (Da)Tools
P10236 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: CB39341C31B768B4

FASTA1,058114,424
        10         20         30         40         50         60 
MGQEDGNRGE RRAAGTPVEV TALYATDGCV ITSSIALLTN SLLGAEPVYI FSYDAYTHDG 

        70         80         90        100        110        120 
RADGPTEQDR FEESRALYQA SGGLNGDSFR VTFCLLGTEV GGTHQARGRT RPMFVCRFER 

       130        140        150        160        170        180 
ADDVAALQDA LAHGTPLQPD HIAATLDAEA TFALHANMIL ALTVAINNAS PRTGRDAAAA 

       190        200        210        220        230        240 
QYDQGASLRS LVGRTSLGQR GLTTLYVHHE VRVLAAYRRA YYGSAQSPFW FLSKFGPDEK 

       250        260        270        280        290        300 
SLVLTTRYYL LQAQRLGGAG ATYDLQAIKD ICATYAIPHA PRPDTVSAAS LTSFAAITRF 

       310        320        330        340        350        360 
CCTSQYARGA AAAGFPLYVE RRIAADVRET SALEKFITHD RSCLRVSDRE FITYIYLAHF 

       370        380        390        400        410        420 
ECFSPPRLAT HLRAVTTHDP NPAASTEQPS PLGREAVEQF FCHVRAQLNI GEYVKHNVTP 

       430        440        450        460        470        480 
RETVLDGDTA KAYLRARTYA PGALTPAPAY CGAVDSATKM MGRLADAEKL LVPRGWPAFA 

       490        500        510        520        530        540 
PASPGEDTAG GTPPPQTCGI VKRLLRLAAT EQQGPTPPAI AALIRNAAVQ TPLPVYRISM 

       550        560        570        580        590        600 
VPTGQAFAAL AWDDWARITR DARLAEAVVS AEAAAHPDHG ALGRRLTDRI RAQGPVMPPG 

       610        620        630        640        650        660 
GLDAGGQMYV NRNEIFNGAL AITNIILDLD IALKEPVPFR RLHEALGHFR RGALAAVQLL 

       670        680        690        700        710        720 
FPAARVDPDA YPCYFFKSAC RPGPASVGSG SGLGNDDDGD WFPCYDDAGD EEWAEDPGAM 

       730        740        750        760        770        780 
DTSHDPPDDE VAYFDLCHEV GPTAEPRETD SPVCSCTDKI GLRVCMPVPA PYVVHGSLTM 

       790        800        810        820        830        840 
RGVARVIQQA VLLDRDFVEA IGSYVKNFLL IDTGVYAHGH SLRLPYFAKI APDGPACGRL 

       850        860        870        880        890        900 
LPVFVIPPAC KDVPAFVAAH ADPRRFHFHA PPTYLASPRE IRVLHSLGGD YVSFFERKAS 

       910        920        930        940        950        960 
RNALEHFGRR ETLTEVLGRY NVQPDAGGTV EGFASELLGR IVACIETHFP EHAGEYQAVS 

       970        980        990       1000       1010       1020 
VRRAVSKDDW VLLQLVPVRG TLQQSLSCLR FKHGRASRAT ARTFVALSVG ANNRLCVSLC 

      1030       1040       1050 
QQCFAAKCDS NRLHTLFTID AGTPCSPSVP CSTSQPSS 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Structures of herpes simplex virus type 1 genes required for replication of virus DNA."
McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D.
J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nonneuroinvasive mutant HF10.
[4]"Herpes simplex virus type 1 bacterial artificial chromosome."
Cunningham C., Davison A.J.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 17 syn+.
[5]"Helicase-primase complex of herpes simplex virus type 1: a mutation in the UL52 subunit abolishes primase activity."
Klinedinst D.K., Challberg M.D.
J. Virol. 68:3693-3701(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32288.1.
M19122 Genomic DNA. Translation: AAA45826.1.
DQ889502 Genomic DNA. Translation: ABI63513.1.
FJ593289 Genomic DNA. Translation: ACM62276.1.
PIRWMBE52. E29890.
RefSeqNP_044655.1. NC_001806.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid971449. 2 interactions.
DIPDIP-1092N.

Chemistry

BindingDBP10236.
ChEMBLCHEMBL4380.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703423.

Phylogenomic databases

ProtClustDBPHA3145.

Family and domain databases

InterProIPR004340. DNA_primase_UL52/UL70_Herpvir.
[Graphical view]
PfamPF03121. Herpes_UL52. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePRIM_HHV11
AccessionPrimary (citable) accession number: P10236
Secondary accession number(s): B9VQI1, Q09I82
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families