ID VHS_HHV11 Reviewed; 489 AA. AC P10225; Q09I92; Q82170; DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1989, sequence version 1. DT 15-JUN-2010, entry version 48. DE RecName: Full=Virion host shutoff protein; DE Short=Vhs; DE EC=3.1.27.-; GN Name=UL41; OS Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus OS 1). OC Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; OC Alphaherpesvirinae; Simplexvirus. OX NCBI_TaxID=10299; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=88274327; PubMed=2839594; RA McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., RA McNab D., Perry L.J., Scott J.E., Taylor P.; RT "The complete DNA sequence of the long unique region in the genome of RT herpes simplex virus type 1."; RL J. Gen. Virol. 69:1531-1574(1988). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=HSZP; RX MEDLINE=98083687; PubMed=9421879; DOI=10.1023/A:1007915025086; RA Vojvodova A., Matis J., Kudelova M., Rajcani J.; RT "Herpes simplex virus type 1 (HSV-1) strain HSZP host shutoff gene: RT nucleotide sequence and comparison with HSV-1 strains differing in RT early shutoff of host protein synthesis."; RL Virus Genes 15:155-159(1997). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=Nonneuroinvasive mutant HF10; RA Nishiyama Y., Ushijima Y.; RT "Sequence analysis of a nonneuroinvasive Herpes simplex virus type 1 RT mutant HF10."; RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases. RN [4] RP FUNCTION. RC STRAIN=F; RX PubMed=12072498; DOI=10.1128/JVI.76.14.6974-6986.2002; RA Trgovcich J., Johnson D., Roizman B.; RT "Cell surface major histocompatibility complex class II proteins are RT regulated by the products of the gamma(1)34.5 and U(L)41 genes of RT herpes simplex virus 1."; RL J. Virol. 76:6974-6986(2002). RN [5] RP FUNCTION. RX PubMed=16940547; DOI=10.1128/JVI.01008-06; RA Taddeo B., Roizman B.; RT "The virion host shutoff protein (UL41) of herpes simplex virus 1 is RT an endoribonuclease with a substrate specificity similar to that of RT RNase A."; RL J. Virol. 80:9341-9345(2006). RN [6] RP FUNCTION. RX PubMed=17093196; DOI=10.1128/JVI.01812-06; RA Read G.S., Patterson M.; RT "Packaging of the virion host shutoff (Vhs) protein of herpes simplex RT virus: two forms of the Vhs polypeptide are associated with RT intranuclear B and C capsids, but only one is associated with RT enveloped virions."; RL J. Virol. 81:1148-1161(2007). CC -!- FUNCTION: Endoribonuclease that is a minor structural component of CC the virion. Following release from infecting virions, accelerates CC the degradation of most cellular mRNAs in the cytoplasm. Together CC with inhibition of host splicing by ICP27, contributes to an CC overall decrease in host protein synthesis. Also, after the onset CC of viral transcription, accelerates the turnover of viral mRNA, CC thereby facilitating the sequential expression of different CC classes of viral genes. Binds human eIF4H, thereby may interact CC directly with the translation initiation complex and thus digest CC specifically mRNAs. Also impedes antigen presentation by major CC histocompatibility complex class I and class II molecules, CC inhibits secretion of cytokines that would otherwise recruit CC lymphocytes and neutrophils cells to the site of infection and CC blocks the activation of dendritic cells. Impedes the alpha/beta CC interferon-mediated response to infection. CC -!- SUBUNIT: Interacts with human EIF4A2 and EIF4H (By similarity). CC -!- SUBCELLULAR LOCATION: Virion (Potential). CC -!- MISCELLANEOUS: Strain 17 is relatively weak regarding early CC shutoff compared to strain KOS. Strain HSZP protein is non CC functionnal for host protein shutoff. CC -!- SIMILARITY: Belongs to the herpesviridae VHS protein family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X14112; CAA32304.1; -; Genomic_DNA. DR EMBL; DQ889502; ABI63503.1; -; Genomic_DNA. DR EMBL; Z72337; CAA96524.1; -; Genomic_DNA. DR PIR; E30088; WMBEF1. DR RefSeq; NP_044643.1; -. DR SMR; P10225; 173-264. DR MINT; MINT-6732794; -. DR GeneID; 2703365; -. DR ProtClustDB; CLSP2510162; -. DR GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. DR GO; GO:0006281; P:DNA repair; IEA:InterPro. DR GO; GO:0030683; P:evasion by virus of host immune response; IEA:UniProtKB-KW. DR InterPro; IPR006086; XPG/RAD2_endonuclease. DR Pfam; PF00867; XPG_I; 1. PE 3: Inferred from homology; KW Complete proteome; Endonuclease; Host-virus interaction; Hydrolase; KW Interferon antiviral system evasion; Late protein; Nuclease; KW Viral immunoevasion; Virion; Virus reference strain. FT CHAIN 1 489 Virion host shutoff protein. FT /FTId=PRO_0000116054. FT VARIANT 18 18 R -> H (in strain: HSZP). FT VARIANT 115 115 V -> A (in strain: HSZP). FT VARIANT 316 316 E -> K (in strain: Nonneuroinvasive FT mutant HF10). FT VARIANT 364 364 P -> S (in strain: Nonneuroinvasive FT mutant HF10). FT VARIANT 374 374 L -> R (in strain: HSZP). FT VARIANT 384 386 NPR -> SRQ (in strain: HSZP). FT VARIANT 385 385 P -> R (in strain: Nonneuroinvasive FT mutant HF10). FT VARIANT 392 392 D -> N (in strain: HSZP). FT VARIANT 452 452 V -> M (in strain: HSZP). SQ SEQUENCE 489 AA; 54918 MW; 3DD706B26A1873BD CRC64; MGLFGMMKFA HTHHLVKRRG LGAPAGYFTP IAVDLWNVMY TLVVKYQRRY PSYDREAITL HCLCRLLKVF TQKSLFPIFV TDRGVNCMEP VVFGAKAILA RTTAQCRTDE EASDVDASPP PSPITDSRPS SAFSNMRRRG TSLASGTRGT AGSGAALPSA APSKPALRLA HLFCIRVLRA LGYAYINSGQ LEADDACANL YHTNTVAYVY TTDTDLLLMG CDIVLDISAC YIPTINCRDI LKYFKMSYPQ FLALFVRCHT DLHPNNTYAS VEDVLRECHW TPPSRSQTRR AIRREHTSSR STETRPPLPP AAGGTETRVS WTEILTQQIA GGYEDDEDLP LDPRDVTGGH PGPRSSSSEI LTPPELVQVP NAQLLEEHRS YVANPRRHVI HDAPESLDWL PDPMTITELV EHRYIKYVIS LIGPKERGPW TLLKRLPIYQ DIRDENLARS IVTRHITAPD IADRFLEQLR TQAPPPAFYK DVLAKFWDE //