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P10225

- SHUT_HHV11

UniProt

P10225 - SHUT_HHV11

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Protein

Virion host shutoff protein

Gene

UL41

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Minor structural protein that acts as an endoribonuclease during lytic infection. Degrades host mRNAs in the cytoplasm by cutting them at preferred sites, including some in regions of translation initiation. Together with inhibition of host splicing by ICP27, contributes to an overall decrease in host protein synthesis. Also, after the onset of viral transcription, accelerates the turnover of viral mRNA, thereby facilitating the sequential expression of different classes of viral genes. Binds translation initiation factors eIF4H, eIF4AI, and eIF4AII, thereby may interact directly with the translation initiation complex and thus digest specifically mRNAs. Also impedes antigen presentation by major histocompatibility complex class I and class II molecules, inhibits secretion of cytokines that would otherwise recruit lymphocytes and neutrophils cells to the site of infection and blocks the activation of dendritic cells. Impedes the alpha/beta interferon-mediated response to infection.6 Publications

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. evasion or tolerance by virus of host immune response Source: UniProtKB-KW
  2. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  3. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Virion host shutoff protein (EC:3.1.27.-)
Short name:
Vhs
Gene namesi
Name:UL41
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

Virion Curated

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Virion host shutoff proteinPRO_0000116054Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with human EIF4H, EIF4A1 and EIF4A2; interaction with eIF4AI and EIF4A2 presumably allows Vhs protein to associate with the eIF4F cap-binding complex.1 Publication

Protein-protein interaction databases

DIPiDIP-57855N.
IntActiP10225. 8 interactions.
MINTiMINT-6732794.

Structurei

3D structure databases

ProteinModelPortaliP10225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae VHS protein family.Curated

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiIPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
[Graphical view]
PfamiPF00867. XPG_I. 1 hit.
[Graphical view]
SUPFAMiSSF88723. SSF88723. 2 hits.

Sequencei

Sequence statusi: Complete.

P10225-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLFGMMKFA HTHHLVKRRG LGAPAGYFTP IAVDLWNVMY TLVVKYQRRY
60 70 80 90 100
PSYDREAITL HCLCRLLKVF TQKSLFPIFV TDRGVNCMEP VVFGAKAILA
110 120 130 140 150
RTTAQCRTDE EASDVDASPP PSPITDSRPS SAFSNMRRRG TSLASGTRGT
160 170 180 190 200
AGSGAALPSA APSKPALRLA HLFCIRVLRA LGYAYINSGQ LEADDACANL
210 220 230 240 250
YHTNTVAYVY TTDTDLLLMG CDIVLDISAC YIPTINCRDI LKYFKMSYPQ
260 270 280 290 300
FLALFVRCHT DLHPNNTYAS VEDVLRECHW TPPSRSQTRR AIRREHTSSR
310 320 330 340 350
STETRPPLPP AAGGTETRVS WTEILTQQIA GGYEDDEDLP LDPRDVTGGH
360 370 380 390 400
PGPRSSSSEI LTPPELVQVP NAQLLEEHRS YVANPRRHVI HDAPESLDWL
410 420 430 440 450
PDPMTITELV EHRYIKYVIS LIGPKERGPW TLLKRLPIYQ DIRDENLARS
460 470 480
IVTRHITAPD IADRFLEQLR TQAPPPAFYK DVLAKFWDE
Length:489
Mass (Da):54,918
Last modified:July 1, 1989 - v1
Checksum:i3DD706B26A1873BD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti18 – 181R → H in strain: HSZP.
Natural varianti115 – 1151V → A in strain: HSZP.
Natural varianti316 – 3161E → K in strain: Nonneuroinvasive mutant HF10.
Natural varianti364 – 3641P → S in strain: Nonneuroinvasive mutant HF10.
Natural varianti374 – 3741L → R in strain: HSZP.
Natural varianti384 – 3863NPR → SRQ in strain: HSZP.
Natural varianti385 – 3851P → R in strain: Nonneuroinvasive mutant HF10.
Natural varianti392 – 3921D → N in strain: HSZP.
Natural varianti452 – 4521V → M in strain: HSZP.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32304.1.
DQ889502 Genomic DNA. Translation: ABI63503.1.
Z72337 Genomic DNA. Translation: CAA96524.1.
PIRiE30088. WMBEF1.
RefSeqiNP_044643.1. NC_001806.1.

Genome annotation databases

GeneIDi2703365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32304.1 .
DQ889502 Genomic DNA. Translation: ABI63503.1 .
Z72337 Genomic DNA. Translation: CAA96524.1 .
PIRi E30088. WMBEF1.
RefSeqi NP_044643.1. NC_001806.1.

3D structure databases

ProteinModelPortali P10225.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-57855N.
IntActi P10225. 8 interactions.
MINTi MINT-6732794.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703365.

Family and domain databases

Gene3Di 3.40.50.1010. 1 hit.
InterProi IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
[Graphical view ]
Pfami PF00867. XPG_I. 1 hit.
[Graphical view ]
SUPFAMi SSF88723. SSF88723. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Herpes simplex virus type 1 (HSV-1) strain HSZP host shutoff gene: nucleotide sequence and comparison with HSV-1 strains differing in early shutoff of host protein synthesis."
    Vojvodova A., Matis J., Kudelova M., Rajcani J.
    Virus Genes 15:155-159(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HSZP.
  3. "Sequence analysis of a nonneuroinvasive Herpes simplex virus type 1 mutant HF10."
    Nishiyama Y., Ushijima Y.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Nonneuroinvasive mutant HF10.
  4. "Cell surface major histocompatibility complex class II proteins are regulated by the products of the gamma(1)34.5 and U(L)41 genes of herpes simplex virus 1."
    Trgovcich J., Johnson D., Roizman B.
    J. Virol. 76:6974-6986(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: F.
  5. "The virion host shutoff protein (UL41) of herpes simplex virus 1 is an endoribonuclease with a substrate specificity similar to that of RNase A."
    Taddeo B., Roizman B.
    J. Virol. 80:9341-9345(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Packaging of the virion host shutoff (Vhs) protein of herpes simplex virus: two forms of the Vhs polypeptide are associated with intranuclear B and C capsids, but only one is associated with enveloped virions."
    Read G.S., Patterson M.
    J. Virol. 81:1148-1161(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Evidence for translational regulation by the herpes simplex virus virion host shutoff protein."
    Saffran H.A., Read G.S., Smiley J.R.
    J. Virol. 84:6041-6049(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The virion host shutoff endonuclease (UL41) of herpes simplex virus interacts with the cellular cap-binding complex eIF4F."
    Page H.G., Read G.S.
    J. Virol. 84:6886-6890(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EIF4H AND EIF4A1.
  9. "mRNA decay during herpes simplex virus (HSV) infections: mutations that affect translation of an mRNA influence the sites at which it is cleaved by the HSV virion host shutoff (Vhs) protein."
    Shiflett L.A., Read G.S.
    J. Virol. 87:94-109(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSHUT_HHV11
AccessioniPrimary (citable) accession number: P10225
Secondary accession number(s): Q09I92, Q82170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Strain 17 is relatively weak regarding early shutoff compared to strain KOS. Strain HSZP protein is non-functional for host protein shutoff.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3