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P10225 (SHUT_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Virion host shutoff protein

Short name=Vhs
EC=3.1.27.-
Gene names
Name:UL41
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Minor structural protein that acts as an endoribonuclease during lytic infection. Degrades host mRNAs in the cytoplasm by cutting them at preferred sites, including some in regions of translation initiation. Together with inhibition of host splicing by ICP27, contributes to an overall decrease in host protein synthesis. Also, after the onset of viral transcription, accelerates the turnover of viral mRNA, thereby facilitating the sequential expression of different classes of viral genes. Binds translation initiation factors eIF4H, eIF4AI, and eIF4AII, thereby may interact directly with the translation initiation complex and thus digest specifically mRNAs. Also impedes antigen presentation by major histocompatibility complex class I and class II molecules, inhibits secretion of cytokines that would otherwise recruit lymphocytes and neutrophils cells to the site of infection and blocks the activation of dendritic cells. Impedes the alpha/beta interferon-mediated response to infection. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure

Interacts with human EIF4H, EIF4A1 and EIF4A2; interaction with eIF4AI and EIF4A2 presumably allows Vhs protein to associate with the eIF4F cap-binding complex Probable. Ref.8

Subcellular location

Virion Potential.

Miscellaneous

Strain 17 is relatively weak regarding early shutoff compared to strain KOS. Strain HSZP protein is non-functional for host protein shutoff.

Sequence similarities

Belongs to the herpesviridae VHS protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Virion host shutoff protein
PRO_0000116054

Natural variations

Natural variant181R → H in strain: HSZP.
Natural variant1151V → A in strain: HSZP.
Natural variant3161E → K in strain: Nonneuroinvasive mutant HF10.
Natural variant3641P → S in strain: Nonneuroinvasive mutant HF10.
Natural variant3741L → R in strain: HSZP.
Natural variant384 – 3863NPR → SRQ in strain: HSZP.
Natural variant3851P → R in strain: Nonneuroinvasive mutant HF10.
Natural variant3921D → N in strain: HSZP.
Natural variant4521V → M in strain: HSZP.

Sequences

Sequence LengthMass (Da)Tools
P10225 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 3DD706B26A1873BD

FASTA48954,918
        10         20         30         40         50         60 
MGLFGMMKFA HTHHLVKRRG LGAPAGYFTP IAVDLWNVMY TLVVKYQRRY PSYDREAITL 

        70         80         90        100        110        120 
HCLCRLLKVF TQKSLFPIFV TDRGVNCMEP VVFGAKAILA RTTAQCRTDE EASDVDASPP 

       130        140        150        160        170        180 
PSPITDSRPS SAFSNMRRRG TSLASGTRGT AGSGAALPSA APSKPALRLA HLFCIRVLRA 

       190        200        210        220        230        240 
LGYAYINSGQ LEADDACANL YHTNTVAYVY TTDTDLLLMG CDIVLDISAC YIPTINCRDI 

       250        260        270        280        290        300 
LKYFKMSYPQ FLALFVRCHT DLHPNNTYAS VEDVLRECHW TPPSRSQTRR AIRREHTSSR 

       310        320        330        340        350        360 
STETRPPLPP AAGGTETRVS WTEILTQQIA GGYEDDEDLP LDPRDVTGGH PGPRSSSSEI 

       370        380        390        400        410        420 
LTPPELVQVP NAQLLEEHRS YVANPRRHVI HDAPESLDWL PDPMTITELV EHRYIKYVIS 

       430        440        450        460        470        480 
LIGPKERGPW TLLKRLPIYQ DIRDENLARS IVTRHITAPD IADRFLEQLR TQAPPPAFYK 


DVLAKFWDE 

« Hide

References

[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Herpes simplex virus type 1 (HSV-1) strain HSZP host shutoff gene: nucleotide sequence and comparison with HSV-1 strains differing in early shutoff of host protein synthesis."
Vojvodova A., Matis J., Kudelova M., Rajcani J.
Virus Genes 15:155-159(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HSZP.
[3]"Sequence analysis of a nonneuroinvasive Herpes simplex virus type 1 mutant HF10."
Nishiyama Y., Ushijima Y.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Nonneuroinvasive mutant HF10.
[4]"Cell surface major histocompatibility complex class II proteins are regulated by the products of the gamma(1)34.5 and U(L)41 genes of herpes simplex virus 1."
Trgovcich J., Johnson D., Roizman B.
J. Virol. 76:6974-6986(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: F.
[5]"The virion host shutoff protein (UL41) of herpes simplex virus 1 is an endoribonuclease with a substrate specificity similar to that of RNase A."
Taddeo B., Roizman B.
J. Virol. 80:9341-9345(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Packaging of the virion host shutoff (Vhs) protein of herpes simplex virus: two forms of the Vhs polypeptide are associated with intranuclear B and C capsids, but only one is associated with enveloped virions."
Read G.S., Patterson M.
J. Virol. 81:1148-1161(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Evidence for translational regulation by the herpes simplex virus virion host shutoff protein."
Saffran H.A., Read G.S., Smiley J.R.
J. Virol. 84:6041-6049(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The virion host shutoff endonuclease (UL41) of herpes simplex virus interacts with the cellular cap-binding complex eIF4F."
Page H.G., Read G.S.
J. Virol. 84:6886-6890(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EIF4H AND EIF4A1.
[9]"mRNA decay during herpes simplex virus (HSV) infections: mutations that affect translation of an mRNA influence the sites at which it is cleaved by the HSV virion host shutoff (Vhs) protein."
Shiflett L.A., Read G.S.
J. Virol. 87:94-109(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32304.1.
DQ889502 Genomic DNA. Translation: ABI63503.1.
Z72337 Genomic DNA. Translation: CAA96524.1.
PIRWMBEF1. E30088.
RefSeqNP_044643.1. NC_001806.1.

3D structure databases

ProteinModelPortalP10225.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP10225. 8 interactions.
MINTMINT-6732794.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703365.

Family and domain databases

Gene3D3.40.50.1010. 1 hit.
InterProIPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
[Graphical view]
PfamPF00867. XPG_I. 1 hit.
[Graphical view]
SUPFAMSSF88723. SSF88723. 2 hits.
ProtoNetSearch...

Entry information

Entry nameSHUT_HHV11
AccessionPrimary (citable) accession number: P10225
Secondary accession number(s): Q09I92, Q82170
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families