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P10225

- SHUT_HHV11

UniProt

P10225 - SHUT_HHV11

Protein

Virion host shutoff protein

Gene

UL41

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Minor structural protein that acts as an endoribonuclease during lytic infection. Degrades host mRNAs in the cytoplasm by cutting them at preferred sites, including some in regions of translation initiation. Together with inhibition of host splicing by ICP27, contributes to an overall decrease in host protein synthesis. Also, after the onset of viral transcription, accelerates the turnover of viral mRNA, thereby facilitating the sequential expression of different classes of viral genes. Binds translation initiation factors eIF4H, eIF4AI, and eIF4AII, thereby may interact directly with the translation initiation complex and thus digest specifically mRNAs. Also impedes antigen presentation by major histocompatibility complex class I and class II molecules, inhibits secretion of cytokines that would otherwise recruit lymphocytes and neutrophils cells to the site of infection and blocks the activation of dendritic cells. Impedes the alpha/beta interferon-mediated response to infection.6 Publications

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. evasion or tolerance by virus of host immune response Source: UniProtKB-KW
    2. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
    3. suppression by virus of host gene expression Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Interferon antiviral system evasion, Viral immunoevasion

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Virion host shutoff protein (EC:3.1.27.-)
    Short name:
    Vhs
    Gene namesi
    Name:UL41
    OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10299 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009294: Genome

    Subcellular locationi

    Virion Curated

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 489489Virion host shutoff proteinPRO_0000116054Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Interacts with human EIF4H, EIF4A1 and EIF4A2; interaction with eIF4AI and EIF4A2 presumably allows Vhs protein to associate with the eIF4F cap-binding complex.1 Publication

    Protein-protein interaction databases

    DIPiDIP-57855N.
    IntActiP10225. 8 interactions.
    MINTiMINT-6732794.

    Structurei

    3D structure databases

    ProteinModelPortaliP10225.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the herpesviridae VHS protein family.Curated

    Family and domain databases

    Gene3Di3.40.50.1010. 1 hit.
    InterProiIPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    [Graphical view]
    PfamiPF00867. XPG_I. 1 hit.
    [Graphical view]
    SUPFAMiSSF88723. SSF88723. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P10225-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLFGMMKFA HTHHLVKRRG LGAPAGYFTP IAVDLWNVMY TLVVKYQRRY    50
    PSYDREAITL HCLCRLLKVF TQKSLFPIFV TDRGVNCMEP VVFGAKAILA 100
    RTTAQCRTDE EASDVDASPP PSPITDSRPS SAFSNMRRRG TSLASGTRGT 150
    AGSGAALPSA APSKPALRLA HLFCIRVLRA LGYAYINSGQ LEADDACANL 200
    YHTNTVAYVY TTDTDLLLMG CDIVLDISAC YIPTINCRDI LKYFKMSYPQ 250
    FLALFVRCHT DLHPNNTYAS VEDVLRECHW TPPSRSQTRR AIRREHTSSR 300
    STETRPPLPP AAGGTETRVS WTEILTQQIA GGYEDDEDLP LDPRDVTGGH 350
    PGPRSSSSEI LTPPELVQVP NAQLLEEHRS YVANPRRHVI HDAPESLDWL 400
    PDPMTITELV EHRYIKYVIS LIGPKERGPW TLLKRLPIYQ DIRDENLARS 450
    IVTRHITAPD IADRFLEQLR TQAPPPAFYK DVLAKFWDE 489
    Length:489
    Mass (Da):54,918
    Last modified:July 1, 1989 - v1
    Checksum:i3DD706B26A1873BD
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti18 – 181R → H in strain: HSZP.
    Natural varianti115 – 1151V → A in strain: HSZP.
    Natural varianti316 – 3161E → K in strain: Nonneuroinvasive mutant HF10.
    Natural varianti364 – 3641P → S in strain: Nonneuroinvasive mutant HF10.
    Natural varianti374 – 3741L → R in strain: HSZP.
    Natural varianti384 – 3863NPR → SRQ in strain: HSZP.
    Natural varianti385 – 3851P → R in strain: Nonneuroinvasive mutant HF10.
    Natural varianti392 – 3921D → N in strain: HSZP.
    Natural varianti452 – 4521V → M in strain: HSZP.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32304.1.
    DQ889502 Genomic DNA. Translation: ABI63503.1.
    Z72337 Genomic DNA. Translation: CAA96524.1.
    PIRiE30088. WMBEF1.
    RefSeqiNP_044643.1. NC_001806.1.

    Genome annotation databases

    GeneIDi2703365.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32304.1 .
    DQ889502 Genomic DNA. Translation: ABI63503.1 .
    Z72337 Genomic DNA. Translation: CAA96524.1 .
    PIRi E30088. WMBEF1.
    RefSeqi NP_044643.1. NC_001806.1.

    3D structure databases

    ProteinModelPortali P10225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-57855N.
    IntActi P10225. 8 interactions.
    MINTi MINT-6732794.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703365.

    Family and domain databases

    Gene3Di 3.40.50.1010. 1 hit.
    InterProi IPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    [Graphical view ]
    Pfami PF00867. XPG_I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF88723. SSF88723. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
      J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Herpes simplex virus type 1 (HSV-1) strain HSZP host shutoff gene: nucleotide sequence and comparison with HSV-1 strains differing in early shutoff of host protein synthesis."
      Vojvodova A., Matis J., Kudelova M., Rajcani J.
      Virus Genes 15:155-159(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HSZP.
    3. "Sequence analysis of a nonneuroinvasive Herpes simplex virus type 1 mutant HF10."
      Nishiyama Y., Ushijima Y.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Nonneuroinvasive mutant HF10.
    4. "Cell surface major histocompatibility complex class II proteins are regulated by the products of the gamma(1)34.5 and U(L)41 genes of herpes simplex virus 1."
      Trgovcich J., Johnson D., Roizman B.
      J. Virol. 76:6974-6986(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: F.
    5. "The virion host shutoff protein (UL41) of herpes simplex virus 1 is an endoribonuclease with a substrate specificity similar to that of RNase A."
      Taddeo B., Roizman B.
      J. Virol. 80:9341-9345(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Packaging of the virion host shutoff (Vhs) protein of herpes simplex virus: two forms of the Vhs polypeptide are associated with intranuclear B and C capsids, but only one is associated with enveloped virions."
      Read G.S., Patterson M.
      J. Virol. 81:1148-1161(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Evidence for translational regulation by the herpes simplex virus virion host shutoff protein."
      Saffran H.A., Read G.S., Smiley J.R.
      J. Virol. 84:6041-6049(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The virion host shutoff endonuclease (UL41) of herpes simplex virus interacts with the cellular cap-binding complex eIF4F."
      Page H.G., Read G.S.
      J. Virol. 84:6886-6890(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EIF4H AND EIF4A1.
    9. "mRNA decay during herpes simplex virus (HSV) infections: mutations that affect translation of an mRNA influence the sites at which it is cleaved by the HSV virion host shutoff (Vhs) protein."
      Shiflett L.A., Read G.S.
      J. Virol. 87:94-109(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSHUT_HHV11
    AccessioniPrimary (citable) accession number: P10225
    Secondary accession number(s): Q09I92, Q82170
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Strain 17 is relatively weak regarding early shutoff compared to strain KOS. Strain HSZP protein is non-functional for host protein shutoff.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3