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P10224 (RIR2_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase small chain

Short name=R2
EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase 38 kDa subunit
Ribonucleotide reductase small subunit
Gene names
ORF Names:UL40
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. The N-terminal region confers antiapoptotic activity in differentiated cells such as neurons and is important for viral reactivation to increase neural survivability By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterotetramer composed of a homodimer of the large subunit UL39 (R1) and a homodimer of the small subunit UL40 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Subcellular location

Host membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 340318Ribonucleoside-diphosphate reductase small chain
PRO_0000190503

Regions

Transmembrane180 – 20021Helical; Potential

Sites

Active site1311 By similarity
Metal binding941Iron 1 By similarity
Metal binding1241Iron 1 By similarity
Metal binding1241Iron 2 By similarity
Metal binding1271Iron 1 By similarity
Metal binding1871Iron 2 By similarity
Metal binding2211Iron 2 By similarity
Metal binding2241Iron 2 By similarity

Natural variations

Natural variant15 – 184LTDQ → HTGH in strain: Nonneuroinvasive mutant HF10.
Natural variant171D → G in strain: 17 syn+.

Sequences

Sequence LengthMass (Da)Tools
P10224 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 4B4ED994BF74FD3F

FASTA34038,019
        10         20         30         40         50         60 
MDSAAPALSP ALTALTDQSA TADLAIQIPK CPDPERYFYT SQCPDINHLR SLSILNRWLE 

        70         80         90        100        110        120 
TELVFVGDEE DVSKLSEGEL SFYRFLFAFL SAADDLVTEN LGGLSGLFEQ KDILHYYVEQ 

       130        140        150        160        170        180 
ECIEVVHSRV YNIIQLVLFH NNDQARREYV AGTINHPAIR AKVDWLEARV RECASVPEKF 

       190        200        210        220        230        240 
ILMILIEGIF FAASFAAIAY LRTNNLLRVT CQSNDLISRD EAVHTTASCY IYNNYLGGHA 

       250        260        270        280        290        300 
KPPPDRVYGL FRQAVEIEIG FIRSQAPTDS HILSPAALAA IENYVRFSAD RLLGLIHMKP 

       310        320        330        340 
LFSAPPPDAS FPLSLMSTDK HTNFFECRST SYAGAVVNDL 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nonneuroinvasive mutant HF10.
[3]"Herpes simplex virus type 1 bacterial artificial chromosome."
Cunningham C., Davison A.J.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 17 syn+.
[4]"Tinkering with a viral ribonucleotide reductase."
Lembo D., Brune W.
Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32303.1.
DQ889502 Genomic DNA. Translation: ABI63502.1.
FJ593289 Genomic DNA. Translation: ACM62263.1.
PIRWMBES7. D30088.
RefSeqNP_044642.1. NC_001806.1.

3D structure databases

ProteinModelPortalP10224.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP10224. 2 interactions.
MINTMINT-6732638.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703364.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_HHV11
AccessionPrimary (citable) accession number: P10224
Secondary accession number(s): B9VQG8, Q09I93
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways