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Protein

Nuclear egress protein 2

Gene

NEC2

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in virion nuclear egress, the first step of virion release from infected cell. Within the host nucleus, NEC1 interacts with the newly formed capsid through the vertexes and directs it to the inner nuclear membrane by associating with NEC2. Induces the budding of the capsid at the inner nuclear membrane as well as its envelopment into the perinuclear space. There, the NEC1/NEC2 complex promotes the fusion of the enveloped capsid with the outer nuclear membrane and the subsequent release of the viral capsid into the cytoplasm where it will reach the secondary budding sites in the host Golgi or trans-Golgi network.UniRule annotation3 Publications

GO - Biological processi

  • exit of virus from host cell nucleus by nuclear egress Source: UniProtKB
  • viral budding from nuclear membrane Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear egress protein 2UniRule annotation
Gene namesi
Name:NEC2UniRule annotation
Ordered Locus Names:UL34
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 251251Perinuclear spaceUniRule annotationAdd
BLAST
Transmembranei252 – 27221HelicalUniRule annotationAdd
BLAST
Topological domaini273 – 2753NuclearUniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Host nucleus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Nuclear egress protein 2PRO_0000116026Add
BLAST

Post-translational modificationi

Phosphorylated by viral kinase US3.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Forms a heterohexameric complex with NEC1. Interacts with glycoprotein D; this interaction recruits glycoprotein D and glycoprotein M to the inner nuclear membrane.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LMNAP025452EBI-7183680,EBI-351935From a different organism.

Protein-protein interaction databases

IntActiP10218. 1 interaction.
MINTiMINT-224104.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 2612Combined sources
Beta strandi31 – 355Combined sources
Beta strandi43 – 453Combined sources
Beta strandi51 – 566Combined sources
Beta strandi61 – 644Combined sources
Helixi66 – 7611Combined sources
Beta strandi84 – 896Combined sources
Beta strandi91 – 10111Combined sources
Beta strandi117 – 1204Combined sources
Beta strandi124 – 1296Combined sources
Helixi130 – 13910Combined sources
Beta strandi150 – 1589Combined sources
Beta strandi161 – 17010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ZXSX-ray2.77A/C15-185[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 18145Interaction with NEC1Add
BLAST

Sequence similaritiesi

Belongs to the herpesviridae NEC2 protein family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_04024. HSV_NEC2. 1 hit.
InterProiIPR007626. Herpesvirus_viron_egress-type.
[Graphical view]
PfamiPF04541. Herpes_U34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLGKPYTG HPGDAFEGLV QRIRLIVPST LRGGDGEAGP YSPSSLPSRC
60 70 80 90 100
AFQFHGHDGS DESFPIEYVL RLMNDWAEVP CNPYLRIQNT GVSVLFQGFF
110 120 130 140 150
HRPHNAPGGA ITPERTNVIL GSTETTGLSL GDLDTIKGRL GLDARPMMAS
160 170 180 190 200
MWISCFVRMP RVQLAFRFMG PEDAGRTRRI LCRAAEQAIT RRRRTRRSRE
210 220 230 240 250
AYGAEAGLGV AGTGFRARGD GFGPLPLLTQ GPSRPWHQAL RGLKHLRIGP
260 270
PALVLAAGLV LGAAIWWVVG AGARL
Length:275
Mass (Da):29,790
Last modified:July 1, 1989 - v1
Checksum:iBA45B62F68838EC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32309.1.
PIRiG30085. WMBEH4.
RefSeqiYP_009137109.1. NC_001806.2.

Genome annotation databases

GeneIDi2703355.
KEGGivg:2703355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32309.1.
PIRiG30085. WMBEH4.
RefSeqiYP_009137109.1. NC_001806.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ZXSX-ray2.77A/C15-185[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10218. 1 interaction.
MINTiMINT-224104.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703355.
KEGGivg:2703355.

Family and domain databases

HAMAPiMF_04024. HSV_NEC2. 1 hit.
InterProiIPR007626. Herpesvirus_viron_egress-type.
[Graphical view]
PfamiPF04541. Herpes_U34. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEC2_HHV11
AccessioniPrimary (citable) accession number: P10218
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.