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P10218 (UL34_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Virion egress protein UL34
Alternative name(s):
Primary envelopment factor UL34
Gene names
Name:UL34
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a major role in virion nuclear egress, the first step of virion release from infected cell. Viral capsids are initially assembled within the nucleus, UL31/UL34 complex induces capsids budding and envelopment into the perinuclear space. Then UL31/UL34 complex promotes fusion of perinuclear virion envelope with the outer nuclear membrane, releasing viral capsid into the cytoplasm where it will engages budding sites in the Golgi or trans-Golgi network. Ref.4 Ref.5 Ref.7

Subunit structure

Forms a complex with UL31, which interacts with glycoprotein D. This interaction recruits glycoprotein D and glycoprotein M to the inner nuclear membrane. Ref.2 Ref.8 Ref.10 Ref.11

Subcellular location

Host nucleus inner membrane; Single-pass membrane protein. Note: Localizes also at the transient membrane of perinuclear virions. Ref.3 Ref.9

Post-translational modification

Phosphorylated by viral kinase US3. Ref.6

Sequence similarities

Belongs to the herpesviridae UL34 family.

Ontologies

Keywords
   Cellular componentHost membrane
Host nucleus
Membrane
   Developmental stageLate protein
   DomainTransmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componenthost cell nuclear inner membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionprotein binding

Inferred from physical interaction Ref.4. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LMNAP025452EBI-7183680,EBI-351935From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Virion egress protein UL34
PRO_0000116026

Regions

Topological domain1 – 251251Perinuclear space Potential
Transmembrane252 – 27221Helical; Potential
Topological domain273 – 2753Nuclear Potential
Region137 – 18145Interaction with UL31

Sequences

Sequence LengthMass (Da)Tools
P10218 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: BA45B62F68838EC3

FASTA27529,790
        10         20         30         40         50         60 
MAGLGKPYTG HPGDAFEGLV QRIRLIVPST LRGGDGEAGP YSPSSLPSRC AFQFHGHDGS 

        70         80         90        100        110        120 
DESFPIEYVL RLMNDWAEVP CNPYLRIQNT GVSVLFQGFF HRPHNAPGGA ITPERTNVIL 

       130        140        150        160        170        180 
GSTETTGLSL GDLDTIKGRL GLDARPMMAS MWISCFVRMP RVQLAFRFMG PEDAGRTRRI 

       190        200        210        220        230        240 
LCRAAEQAIT RRRRTRRSRE AYGAEAGLGV AGTGFRARGD GFGPLPLLTQ GPSRPWHQAL 

       250        260        270 
RGLKHLRIGP PALVLAAGLV LGAAIWWVVG AGARL 

« Hide

References

[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"U(L)31 and U(L)34 proteins of herpes simplex virus type 1 form a complex that accumulates at the nuclear rim and is required for envelopment of nucleocapsids."
Reynolds A.E., Ryckman B.J., Baines J.D., Zhou Y., Liang L., Roller R.J.
J. Virol. 75:8803-8817(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL31.
[3]"Ultrastructural localization of the herpes simplex virus type 1 UL31, UL34, and US3 proteins suggests specific roles in primary envelopment and egress of nucleocapsids."
Reynolds A.E., Wills E.G., Roller R.J., Ryckman B.J., Baines J.D.
J. Virol. 76:8939-8952(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Conformational changes in the nuclear lamina induced by herpes simplex virus type 1 require genes U(L)31 and U(L)34."
Reynolds A.E., Liang L., Baines J.D.
J. Virol. 78:5564-5575(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Effects of charged cluster mutations on the function of herpes simplex virus type 1 UL34 protein."
Bjerke S.L., Cowan J.M., Kerr J.K., Reynolds A.E., Baines J.D., Roller R.J.
J. Virol. 77:7601-7610(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Herpes simplex virus type 1 primary envelopment: UL34 protein modification and the US3-UL34 catalytic relationship."
Ryckman B.J., Roller R.J.
J. Virol. 78:399-412(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[7]"Herpes simplex virus 1 U(L)31 and U(L)34 gene products promote the late maturation of viral replication compartments to the nuclear periphery."
Simpson-Holley M., Baines J., Roller R., Knipe D.M.
J. Virol. 78:5591-5600(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Identification of an essential domain in the herpes simplex virus 1 UL34 protein that is necessary and sufficient to interact with UL31 protein."
Liang L., Baines J.D.
J. Virol. 79:3797-3806(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL31.
[9]"Herpes simplex virus 1-encoded protein kinase UL13 phosphorylates viral Us3 protein kinase and regulates nuclear localization of viral envelopment factors UL34 and UL31."
Kato A., Yamamoto M., Ohno T., Tanaka M., Sata T., Nishiyama Y., Kawaguchi Y.
J. Virol. 80:1476-1486(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Common and specific properties of herpesvirus UL34/UL31 protein family members revealed by protein complementation assay."
Schnee M., Ruzsics Z., Bubeck A., Koszinowski U.H.
J. Virol. 80:11658-11666(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL31.
[11]"The U(L)31 and U(L)34 gene products of herpes simplex virus 1 are required for optimal localization of viral glycoproteins D and M to the inner nuclear membranes of infected cells."
Wills E., Mou F., Baines J.D.
J. Virol. 83:4800-4809(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GLYCOPROTEIN D.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32309.1.
PIRWMBEH4. G30085.
RefSeqNP_044636.1. NC_001806.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP10218. 1 interaction.
MINTMINT-224104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703355.

Family and domain databases

InterProIPR007626. Herpesvirus_viron_egress-type.
[Graphical view]
PfamPF04541. Herpes_U34. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUL34_HHV11
AccessionPrimary (citable) accession number: P10218
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families