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P10218

- UL34_HHV11

UniProt

P10218 - UL34_HHV11

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Protein

Virion egress protein UL34

Gene

UL34

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Plays a major role in virion nuclear egress, the first step of virion release from infected cell. Viral capsids are initially assembled within the nucleus, UL31/UL34 complex induces capsids budding and envelopment into the perinuclear space. Then UL31/UL34 complex promotes fusion of perinuclear virion envelope with the outer nuclear membrane, releasing viral capsid into the cytoplasm where it will engages budding sites in the Golgi or trans-Golgi network.3 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
Virion egress protein UL34
Alternative name(s):
Primary envelopment factor UL34
Gene namesi
Name:UL34
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

Host nucleus inner membrane 2 Publications; Single-pass membrane protein 2 Publications
Note: Localizes also at the transient membrane of perinuclear virions.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 251251Perinuclear spaceSequence AnalysisAdd
BLAST
Transmembranei252 – 27221HelicalSequence AnalysisAdd
BLAST
Topological domaini273 – 2753NuclearSequence Analysis

GO - Cellular componenti

  1. host cell membrane Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Host nucleus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Virion egress protein UL34PRO_0000116026Add
BLAST

Post-translational modificationi

Phosphorylated by viral kinase US3.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Forms a complex with UL31, which interacts with glycoprotein D. This interaction recruits glycoprotein D and glycoprotein M to the inner nuclear membrane.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LMNAP025452EBI-7183680,EBI-351935From a different organism.

Protein-protein interaction databases

IntActiP10218. 1 interaction.
MINTiMINT-224104.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 18145Interaction with UL31Add
BLAST

Sequence similaritiesi

Belongs to the herpesviridae UL34 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR007626. Herpesvirus_viron_egress-type.
[Graphical view]
PfamiPF04541. Herpes_U34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10218-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGLGKPYTG HPGDAFEGLV QRIRLIVPST LRGGDGEAGP YSPSSLPSRC
60 70 80 90 100
AFQFHGHDGS DESFPIEYVL RLMNDWAEVP CNPYLRIQNT GVSVLFQGFF
110 120 130 140 150
HRPHNAPGGA ITPERTNVIL GSTETTGLSL GDLDTIKGRL GLDARPMMAS
160 170 180 190 200
MWISCFVRMP RVQLAFRFMG PEDAGRTRRI LCRAAEQAIT RRRRTRRSRE
210 220 230 240 250
AYGAEAGLGV AGTGFRARGD GFGPLPLLTQ GPSRPWHQAL RGLKHLRIGP
260 270
PALVLAAGLV LGAAIWWVVG AGARL
Length:275
Mass (Da):29,790
Last modified:July 1, 1989 - v1
Checksum:iBA45B62F68838EC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32309.1.
PIRiG30085. WMBEH4.
RefSeqiNP_044636.1. NC_001806.1.

Genome annotation databases

GeneIDi2703355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32309.1 .
PIRi G30085. WMBEH4.
RefSeqi NP_044636.1. NC_001806.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P10218. 1 interaction.
MINTi MINT-224104.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703355.

Family and domain databases

InterProi IPR007626. Herpesvirus_viron_egress-type.
[Graphical view ]
Pfami PF04541. Herpes_U34. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "U(L)31 and U(L)34 proteins of herpes simplex virus type 1 form a complex that accumulates at the nuclear rim and is required for envelopment of nucleocapsids."
    Reynolds A.E., Ryckman B.J., Baines J.D., Zhou Y., Liang L., Roller R.J.
    J. Virol. 75:8803-8817(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL31.
  3. "Ultrastructural localization of the herpes simplex virus type 1 UL31, UL34, and US3 proteins suggests specific roles in primary envelopment and egress of nucleocapsids."
    Reynolds A.E., Wills E.G., Roller R.J., Ryckman B.J., Baines J.D.
    J. Virol. 76:8939-8952(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Conformational changes in the nuclear lamina induced by herpes simplex virus type 1 require genes U(L)31 and U(L)34."
    Reynolds A.E., Liang L., Baines J.D.
    J. Virol. 78:5564-5575(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Effects of charged cluster mutations on the function of herpes simplex virus type 1 UL34 protein."
    Bjerke S.L., Cowan J.M., Kerr J.K., Reynolds A.E., Baines J.D., Roller R.J.
    J. Virol. 77:7601-7610(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Herpes simplex virus type 1 primary envelopment: UL34 protein modification and the US3-UL34 catalytic relationship."
    Ryckman B.J., Roller R.J.
    J. Virol. 78:399-412(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  7. "Herpes simplex virus 1 U(L)31 and U(L)34 gene products promote the late maturation of viral replication compartments to the nuclear periphery."
    Simpson-Holley M., Baines J., Roller R., Knipe D.M.
    J. Virol. 78:5591-5600(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Identification of an essential domain in the herpes simplex virus 1 UL34 protein that is necessary and sufficient to interact with UL31 protein."
    Liang L., Baines J.D.
    J. Virol. 79:3797-3806(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL31.
  9. "Herpes simplex virus 1-encoded protein kinase UL13 phosphorylates viral Us3 protein kinase and regulates nuclear localization of viral envelopment factors UL34 and UL31."
    Kato A., Yamamoto M., Ohno T., Tanaka M., Sata T., Nishiyama Y., Kawaguchi Y.
    J. Virol. 80:1476-1486(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Common and specific properties of herpesvirus UL34/UL31 protein family members revealed by protein complementation assay."
    Schnee M., Ruzsics Z., Bubeck A., Koszinowski U.H.
    J. Virol. 80:11658-11666(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL31.
  11. "The U(L)31 and U(L)34 gene products of herpes simplex virus 1 are required for optimal localization of viral glycoproteins D and M to the inner nuclear membranes of infected cells."
    Wills E., Mou F., Baines J.D.
    J. Virol. 83:4800-4809(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GLYCOPROTEIN D.

Entry informationi

Entry nameiUL34_HHV11
AccessioniPrimary (citable) accession number: P10218
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3