P10215 (UL31_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Virion egress protein UL31

Alternative name(s):
Primary envelopment factor UL31

Gene names

Name:

UL31

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a major role in virion nuclear egress, the first step of virion release from infected cell. Viral capsids are initially assembled within the nucleus, UL31/UL34 complex induces capsids budding and envelopment into the perinuclear space. Then UL31/UL34 complex promotes fusion of perinuclear virion envelope with the outer nuclear membrane, releasing viral capsid into the cytoplasm where it will engages budding sites in the Golgi or trans-Golgi network. Ref.4 Ref.5
Subunit structure	Forms a complex with UL34, which interacts with glycoprotein D. This interaction recruits glycoprotein D and glycoprotein M to the inner nuclear membrane. Ref.2 Ref.6 Ref.7 Ref.10
Subcellular location	Host nucleus inner membrane. Note: Remains attached to the nucleus inner membrane through interaction with UL34. Localizes also at the transient membrane of perinuclear virions. Ref.3 Ref.8
Post-translational modification	Phosphorylated on N-terminus serine residues by viral kinase US3. This phosphorylation regulates the localization within the inner nuclear membrane. Ref.9
Sequence similarities	Belongs to the herpesviridae UL31 family.

Ontologies

Keywords
Cellular component	Host membrane Host nucleus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	host cell nuclear inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Virion egress protein UL31		PRO_0000116006

Sequences

	Sequence		Length	Mass (Da)
	P10215 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: D4FE0FD98D934C46	FASTA	306	33,953
	10 20 30 40 50 60 MYDTDPHRRG SRPGPYHGKE RRRSRSSAAG GTLGVVRRAS RKSLPPHARK QELCLHERQR 70 80 90 100 110 120 YRGLFAALAQ TPSEEIAIVR SLSVPLVKTT PVSLPFCLDQ TVADNCLTLS GMGYYLGIGG 130 140 150 160 170 180 CCPACNAGDG RFAATSREAL ILAFVQQINT IFEHRAFLAS LVVLADRHNA PLQDLLAGIL 190 200 210 220 230 240 GQPELFFVHT ILRGGGACDP RLLFYPDPTY GGHMLYVIFP GTSAHLHYRL IDRMLTACPG 250 260 270 280 290 300 YRFVAHVWQS TFVLVVRRNA EKPTDAEIPT VSAADIYCKM RDISFDGGLM LEYQRLYATF DEFPPP

References

[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"U(L)31 and U(L)34 proteins of herpes simplex virus type 1 form a complex that accumulates at the nuclear rim and is required for envelopment of nucleocapsids." Reynolds A.E., Ryckman B.J., Baines J.D., Zhou Y., Liang L., Roller R.J. J. Virol. 75:8803-8817(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL34.
[3]	"Ultrastructural localization of the herpes simplex virus type 1 UL31, UL34, and US3 proteins suggests specific roles in primary envelopment and egress of nucleocapsids." Reynolds A.E., Wills E.G., Roller R.J., Ryckman B.J., Baines J.D. J. Virol. 76:8939-8952(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Conformational changes in the nuclear lamina induced by herpes simplex virus type 1 require genes U(L)31 and U(L)34." Reynolds A.E., Liang L., Baines J.D. J. Virol. 78:5564-5575(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Herpes simplex virus 1 U(L)31 and U(L)34 gene products promote the late maturation of viral replication compartments to the nuclear periphery." Simpson-Holley M., Baines J., Roller R., Knipe D.M. J. Virol. 78:5591-5600(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Identification of an essential domain in the herpes simplex virus 1 UL34 protein that is necessary and sufficient to interact with UL31 protein." Liang L., Baines J.D. J. Virol. 79:3797-3806(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL34.
[7]	"Common and specific properties of herpesvirus UL34/UL31 protein family members revealed by protein complementation assay." Schnee M., Ruzsics Z., Bubeck A., Koszinowski U.H. J. Virol. 80:11658-11666(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL34.
[8]	"Herpes simplex virus 1-encoded protein kinase UL13 phosphorylates viral Us3 protein kinase and regulates nuclear localization of viral envelopment factors UL34 and UL31." Kato A., Yamamoto M., Ohno T., Tanaka M., Sata T., Nishiyama Y., Kawaguchi Y. J. Virol. 80:1476-1486(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Phosphorylation of the UL31 protein of herpes simplex virus 1 by the US3-encoded kinase regulates localization of the nuclear envelopment complex and egress of nucleocapsids." Mou F., Wills E., Baines J.D. J. Virol. 83:5181-5191(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
[10]	"The U(L)31 and U(L)34 gene products of herpes simplex virus 1 are required for optimal localization of viral glycoproteins D and M to the inner nuclear membranes of infected cells." Wills E., Mou F., Baines J.D. J. Virol. 83:4800-4809(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GLYCOPROTEIN D.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32324.1.
PIR	WMBEH1. D30085.
RefSeq	NP_044633.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-224098.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703350.
Phylogenomic databases
ProtClustDB	PHA3328.
Family and domain databases
InterPro	IPR021152. Herpes_UL31. [Graphical view]
Pfam	PF02718. Herpes_UL31. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UL31_HHV11

Accession

Primary (citable) accession number: P10215

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program