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P10210

- SCAF_HHV11

UniProt

P10210 - SCAF_HHV11

Protein

Capsid scaffolding protein

Gene

UL26

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Capsid scaffolding protein acts as a scaffold protein by binding major capsid protein VP5 in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein VP5. Cleavages products are evicted from the capsid before or during DNA packaging By similarity.By similarity
    Assemblin is a protease essential for virion assembly in the nucleus. Catalyzes the cleavage of the assembly protein after complete capsid formation. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging By similarity.By similarity
    Assembly protein plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein VP5. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging By similarity.By similarity

    Catalytic activityi

    Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei61 – 611Charge relay systemBy similarity
    Active sitei129 – 1291Charge relay systemBy similarity
    Active sitei148 – 1481Charge relay systemBy similarity
    Sitei247 – 2482Cleavage; by assemblin; Release siteBy similarity
    Sitei610 – 6112Cleavage; by assemblin; Tail siteBy similarity

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. viral release from host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Viral capsid assembly, Virus exit from host cell

    Protein family/group databases

    MEROPSiS21.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Capsid scaffolding protein
    Alternative name(s):
    Capsid protein P40
    Protease precursor
    Short name:
    pPR
    Virion structural protein UL26
    Cleaved into the following 2 chains:
    Alternative name(s):
    Capsid protein VP24
    Protease
    Alternative name(s):
    Capsid protein VP22A
    Gene namesi
    Name:UL26
    OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10299 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009294: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 635635Capsid scaffolding proteinPRO_0000027244Add
    BLAST
    Chaini1 – 247247AssemblinPRO_0000027245Add
    BLAST
    Chaini248 – 635388Assembly proteinPRO_0000027246Add
    BLAST

    Post-translational modificationi

    Capsid scaffolding protein is cleaved by assemblin after completion of icosahedral capsid formation. Cleavages occur at two or more sites: release and tail site By similarity.By similarity
    Assembly protein is phosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Capsid scaffolding protein homomultimerizes and interacts with major capsid protein VP5. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein VP5 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-8621986,EBI-8621986
    UL19P064913EBI-8621986,EBI-7608705

    Protein-protein interaction databases

    DIPiDIP-1096N.
    IntActiP10210. 1 interaction.
    MINTiMINT-6732542.

    Structurei

    3D structure databases

    ProteinModelPortaliP10210.
    SMRiP10210. Positions 17-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni324 – 34320Interaction with pAPAdd
    BLAST
    Regioni618 – 63518Interaction with major capsid proteinAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi426 – 4294Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi514 – 59077Pro-richAdd
    BLAST

    Domaini

    Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD) By similarity.By similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.16.10. 1 hit.
    InterProiIPR001847. Peptidase_S21.
    [Graphical view]
    PfamiPF00716. Peptidase_S21. 1 hit.
    [Graphical view]
    PRINTSiPR00236. HSVCAPSIDP40.
    SUPFAMiSSF50789. SSF50789. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform Capsid scaffolding protein (identifier: P10210-1) [UniParc]FASTAAdd to Basket

    Also known as: pPR, UL26

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAADAPGDRM EEPLPDRAVP IYVAGFLALY DSGDSGELAL DPDTVRAALP    50
    PDNPLPINVD HRAGCEVGRV LAVVDDPRGP FFVGLIACVQ LERVLETAAS 100
    AAIFERRGPP LSREERLLYL ITNYLPSVSL ATKRLGGEAH PDRTLFAHVA 150
    LCAIGRRLGT IVTYDTGLDA AIAPFRHLSP ASREGARRLA AEAELALSGR 200
    TWAPGVEALT HTLLSTAVNN MMLRDRWSLV AERRRQAGIA GHTYLQASEK 250
    FKMWGAEPVS APARGYKNGA PESTDIPPGS IAAAPQGDRC PIVRQRGVAL 300
    SPVLPPMNPV PTSGTPAPAP PGDGSYLWIP ASHYNQLVAG HAAPQPQPHS 350
    AFGFPAAAGS VAYGPHGAGL SQHYPPHVAH QYPGVLFSGP SPLEAQIAAL 400
    VGAIAADRQA GGQPAAGDPG VRGSGKRRRY EAGPSESYCD QDEPDADYPY 450
    YPGEARGAPR GVDSRRAARH SPGTNETITA LMGAVTSLQQ ELAHMRARTS 500
    APYGMYTPVA HYRPQVGEPE PTTTHPALCP PEAVYRPPPH SAPYGPPQGP 550
    ASHAPTPPYA PAACPPGPPP PPCPSTQTRA PLPTEPAFPP AATGSQPEAS 600
    NAEAGALVNA SSAAHVDVDT ARAADLFVSQ MMGAR 635
    Length:635
    Mass (Da):66,471
    Last modified:July 1, 1989 - v1
    Checksum:iF3B3C7D42F3D062D
    GO
    Isoform pAP (identifier: P10210-2) [UniParc]FASTAAdd to Basket

    Also known as: Assembly protein, UL26.5 protein

    The sequence of this isoform differs from the canonical sequence as follows:
         1-306: Missing.

    Show »
    Length:329
    Mass (Da):33,760
    Checksum:iDF690388D0630F9D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 306306Missing in isoform pAP. CuratedVSP_037414Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32318.1.
    X14112 Genomic DNA. Translation: CAA32319.1.
    PIRiH30084. WMBEW6.
    RefSeqiNP_044627.1. NC_001806.1.
    NP_044628.1. NC_001806.1.

    Genome annotation databases

    GeneIDi2703453.
    2703454.

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32318.1 .
    X14112 Genomic DNA. Translation: CAA32319.1 .
    PIRi H30084. WMBEW6.
    RefSeqi NP_044627.1. NC_001806.1.
    NP_044628.1. NC_001806.1.

    3D structure databases

    ProteinModelPortali P10210.
    SMRi P10210. Positions 17-247.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-1096N.
    IntActi P10210. 1 interaction.
    MINTi MINT-6732542.

    Chemistry

    BindingDBi P10210.
    ChEMBLi CHEMBL3518.

    Protein family/group databases

    MEROPSi S21.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703453.
    2703454.

    Family and domain databases

    Gene3Di 3.20.16.10. 1 hit.
    InterProi IPR001847. Peptidase_S21.
    [Graphical view ]
    Pfami PF00716. Peptidase_S21. 1 hit.
    [Graphical view ]
    PRINTSi PR00236. HSVCAPSIDP40.
    SUPFAMi SSF50789. SSF50789. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
      J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of genes encoding two capsid proteins (VP24 and VP26) of herpes simplex virus type 1."
      Davison M.D., Rixon F.J., Davison A.J.
      J. Gen. Virol. 73:2709-2713(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 11-29; 77-91 AND 223-241.

    Entry informationi

    Entry nameiSCAF_HHV11
    AccessioniPrimary (citable) accession number: P10210
    Secondary accession number(s): O09798
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3