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Protein

Capsid scaffolding protein

Gene

UL26

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation
Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation
Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation

Catalytic activityi

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei61Charge relay systemUniRule annotation1
Active sitei129Charge relay systemUniRule annotation1
Active sitei148Charge relay systemUniRule annotation1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • nuclear capsid assembly Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processViral capsid assembly, Viral release from host cell

Protein family/group databases

MEROPSiS21.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid scaffolding proteinUniRule annotation
Alternative name(s):
Capsid protein P40
Protease precursorUniRule annotation
Short name:
pPRUniRule annotation
Virion structural protein UL26
Cleaved into the following 2 chains:
AssemblinUniRule annotation (EC:3.4.21.97UniRule annotation)
Alternative name(s):
ProteaseUniRule annotation
Assembly proteinUniRule annotation
Alternative name(s):
Capsid assembly proteinUniRule annotation
Gene namesi
Name:UL26
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

Capsid scaffolding protein :
  • Host cytoplasm UniRule annotation
Assemblin :
  • Host nucleus UniRule annotation
Assembly protein :
  • Host nucleus UniRule annotation

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB-SubCell
  • host cell nucleus Source: CAFA

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3518.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000272441 – 635Capsid scaffolding proteinAdd BLAST635
ChainiPRO_00000272451 – 247AssemblinUniRule annotationAdd BLAST247
ChainiPRO_0000027246248 – 635Assembly proteinUniRule annotationAdd BLAST388

Post-translational modificationi

Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release and tail site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei247 – 248Cleavage; by assemblin; Release siteUniRule annotation2
Sitei610 – 611Cleavage; by assemblin; Tail siteUniRule annotation2

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP10210.

Interactioni

Subunit structurei

Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein.UniRule annotation

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi971467. 1 interactor.
DIPiDIP-1096N.
IntActiP10210. 1 interactor.
MINTiMINT-6732542.

Chemistry databases

BindingDBiP10210.

Structurei

3D structure databases

ProteinModelPortaliP10210.
SMRiP10210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni324 – 343Interaction with pAPUniRule annotationAdd BLAST20
Regioni615 – 635Interaction with major capsid proteinUniRule annotationAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi426 – 429Nuclear localization signalBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi514 – 590Pro-richAdd BLAST77

Domaini

Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).UniRule annotation

Sequence similaritiesi

Belongs to the herpesviridae capsid scaffolding protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900004L.

Family and domain databases

Gene3Di3.20.16.10. 1 hit.
HAMAPiMF_04008. HSV_SCAF. 1 hit.
InterProiView protein in InterPro
IPR035443. Herpes_virus/Caudovirus_dom_sf.
IPR001847. Peptidase_S21.
PfamiView protein in Pfam
PF00716. Peptidase_S21. 1 hit.
PRINTSiPR00236. HSVCAPSIDP40.
SUPFAMiSSF50789. SSF50789. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform Capsid scaffolding protein (identifier: P10210-1) [UniParc]FASTAAdd to basket
Also known as: pPR, UL26

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAADAPGDRM EEPLPDRAVP IYVAGFLALY DSGDSGELAL DPDTVRAALP
60 70 80 90 100
PDNPLPINVD HRAGCEVGRV LAVVDDPRGP FFVGLIACVQ LERVLETAAS
110 120 130 140 150
AAIFERRGPP LSREERLLYL ITNYLPSVSL ATKRLGGEAH PDRTLFAHVA
160 170 180 190 200
LCAIGRRLGT IVTYDTGLDA AIAPFRHLSP ASREGARRLA AEAELALSGR
210 220 230 240 250
TWAPGVEALT HTLLSTAVNN MMLRDRWSLV AERRRQAGIA GHTYLQASEK
260 270 280 290 300
FKMWGAEPVS APARGYKNGA PESTDIPPGS IAAAPQGDRC PIVRQRGVAL
310 320 330 340 350
SPVLPPMNPV PTSGTPAPAP PGDGSYLWIP ASHYNQLVAG HAAPQPQPHS
360 370 380 390 400
AFGFPAAAGS VAYGPHGAGL SQHYPPHVAH QYPGVLFSGP SPLEAQIAAL
410 420 430 440 450
VGAIAADRQA GGQPAAGDPG VRGSGKRRRY EAGPSESYCD QDEPDADYPY
460 470 480 490 500
YPGEARGAPR GVDSRRAARH SPGTNETITA LMGAVTSLQQ ELAHMRARTS
510 520 530 540 550
APYGMYTPVA HYRPQVGEPE PTTTHPALCP PEAVYRPPPH SAPYGPPQGP
560 570 580 590 600
ASHAPTPPYA PAACPPGPPP PPCPSTQTRA PLPTEPAFPP AATGSQPEAS
610 620 630
NAEAGALVNA SSAAHVDVDT ARAADLFVSQ MMGAR
Length:635
Mass (Da):66,471
Last modified:July 1, 1989 - v1
Checksum:iF3B3C7D42F3D062D
GO
Isoform pAP (identifier: P10210-2) [UniParc]FASTAAdd to basket
Also known as: Assembly protein, UL26.5 protein

The sequence of this isoform differs from the canonical sequence as follows:
     1-306: Missing.

Show »
Length:329
Mass (Da):33,760
Checksum:iDF690388D0630F9D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0374141 – 306Missing in isoform pAP. CuratedAdd BLAST306

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32318.1.
X14112 Genomic DNA. Translation: CAA32319.1.
PIRiH30084. WMBEW6.

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Entry informationi

Entry nameiSCAF_HHV11
AccessioniPrimary (citable) accession number: P10210
Secondary accession number(s): O09798
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 22, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families