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P10210 (SCAF_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Capsid scaffolding protein
Alternative name(s):
Capsid protein P40
Protease precursor
Short name=pPR
Virion structural protein UL26

Cleaved into the following 2 chains:

  1. Assemblin
    EC=3.4.21.97
    Alternative name(s):
    Capsid protein VP24
    Protease
  2. Assembly protein
    Alternative name(s):
    Capsid protein VP22A
Gene names
Name:UL26
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length635 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Capsid scaffolding protein acts as a scaffold protein by binding major capsid protein VP5 in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein VP5. Cleavages products are evicted from the capsid before or during DNA packaging By similarity.

Assemblin is a protease essential for virion assembly in the nucleus. Catalyzes the cleavage of the assembly protein after complete capsid formation. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging By similarity.

Assembly protein plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein VP5. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging By similarity.

Catalytic activity

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.

Subunit structure

Capsid scaffolding protein homomultimerizes and interacts with major capsid protein VP5. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein VP5 By similarity.

Subcellular location

Capsid scaffolding protein: Host cytoplasm By similarity.

Assemblin: Host nucleus By similarity.

Assembly protein: Host nucleus By similarity.

Domain

Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD) By similarity.

Post-translational modification

Capsid scaffolding protein is cleaved by assemblin after completion of icosahedral capsid formation. Cleavages occur at two or more sites: release and tail site By similarity.

Assembly protein is phosphorylated.

Sequence similarities

Belongs to the herpesviridae capsid scaffolding protein family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-8621986,EBI-8621986
UL19P064913EBI-8621986,EBI-7608705

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]
Isoform Capsid scaffolding protein (identifier: P10210-1)

Also known as: pPR; UL26;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform pAP (identifier: P10210-2)

Also known as: Assembly protein; UL26.5 protein;

The sequence of this isoform differs from the canonical sequence as follows:
     1-306: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 635635Capsid scaffolding protein
PRO_0000027244
Chain1 – 247247Assemblin
PRO_0000027245
Chain248 – 635388Assembly protein
PRO_0000027246

Regions

Region324 – 34320Interaction with pAP
Region618 – 63518Interaction with major capsid protein
Motif426 – 4294Nuclear localization signal Potential
Compositional bias514 – 59077Pro-rich

Sites

Active site611Charge relay system By similarity
Active site1291Charge relay system By similarity
Active site1481Charge relay system By similarity
Site247 – 2482Cleavage; by assemblin; Release site By similarity
Site610 – 6112Cleavage; by assemblin; Tail site By similarity

Natural variations

Alternative sequence1 – 306306Missing in isoform pAP.
VSP_037414

Sequences

Sequence LengthMass (Da)Tools
Isoform Capsid scaffolding protein (pPR) (UL26) [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: F3B3C7D42F3D062D

FASTA63566,471
        10         20         30         40         50         60 
MAADAPGDRM EEPLPDRAVP IYVAGFLALY DSGDSGELAL DPDTVRAALP PDNPLPINVD 

        70         80         90        100        110        120 
HRAGCEVGRV LAVVDDPRGP FFVGLIACVQ LERVLETAAS AAIFERRGPP LSREERLLYL 

       130        140        150        160        170        180 
ITNYLPSVSL ATKRLGGEAH PDRTLFAHVA LCAIGRRLGT IVTYDTGLDA AIAPFRHLSP 

       190        200        210        220        230        240 
ASREGARRLA AEAELALSGR TWAPGVEALT HTLLSTAVNN MMLRDRWSLV AERRRQAGIA 

       250        260        270        280        290        300 
GHTYLQASEK FKMWGAEPVS APARGYKNGA PESTDIPPGS IAAAPQGDRC PIVRQRGVAL 

       310        320        330        340        350        360 
SPVLPPMNPV PTSGTPAPAP PGDGSYLWIP ASHYNQLVAG HAAPQPQPHS AFGFPAAAGS 

       370        380        390        400        410        420 
VAYGPHGAGL SQHYPPHVAH QYPGVLFSGP SPLEAQIAAL VGAIAADRQA GGQPAAGDPG 

       430        440        450        460        470        480 
VRGSGKRRRY EAGPSESYCD QDEPDADYPY YPGEARGAPR GVDSRRAARH SPGTNETITA 

       490        500        510        520        530        540 
LMGAVTSLQQ ELAHMRARTS APYGMYTPVA HYRPQVGEPE PTTTHPALCP PEAVYRPPPH 

       550        560        570        580        590        600 
SAPYGPPQGP ASHAPTPPYA PAACPPGPPP PPCPSTQTRA PLPTEPAFPP AATGSQPEAS 

       610        620        630 
NAEAGALVNA SSAAHVDVDT ARAADLFVSQ MMGAR 

« Hide

Isoform pAP (Assembly protein) (UL26.5 protein) [UniParc].

Checksum: DF690388D0630F9D
Show »

FASTA32933,760

References

[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of genes encoding two capsid proteins (VP24 and VP26) of herpes simplex virus type 1."
Davison M.D., Rixon F.J., Davison A.J.
J. Gen. Virol. 73:2709-2713(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 11-29; 77-91 AND 223-241.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32318.1.
X14112 Genomic DNA. Translation: CAA32319.1.
PIRWMBEW6. H30084.
RefSeqNP_044627.1. NC_001806.1.
NP_044628.1. NC_001806.1.

3D structure databases

ProteinModelPortalP10210.
SMRP10210. Positions 17-247.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-1096N.
IntActP10210. 1 interaction.
MINTMINT-6732542.

Chemistry

BindingDBP10210.
ChEMBLCHEMBL3518.

Protein family/group databases

MEROPSS21.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703453.
2703454.

Family and domain databases

Gene3D3.20.16.10. 1 hit.
InterProIPR001847. Peptidase_S21.
[Graphical view]
PfamPF00716. Peptidase_S21. 1 hit.
[Graphical view]
PRINTSPR00236. HSVCAPSIDP40.
SUPFAMSSF50789. SSF50789. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSCAF_HHV11
AccessionPrimary (citable) accession number: P10210
Secondary accession number(s): O09798
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families