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P10210

- SCAF_HHV11

UniProt

P10210 - SCAF_HHV11

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Protein

Capsid scaffolding protein

Gene
UL26
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Capsid scaffolding protein acts as a scaffold protein by binding major capsid protein VP5 in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein VP5. Cleavages products are evicted from the capsid before or during DNA packaging By similarity.
Assemblin is a protease essential for virion assembly in the nucleus. Catalyzes the cleavage of the assembly protein after complete capsid formation. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging By similarity.
Assembly protein plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein VP5. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging By similarity.

Catalytic activityi

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611Charge relay system By similarity
Active sitei129 – 1291Charge relay system By similarity
Active sitei148 – 1481Charge relay system By similarity
Sitei247 – 2482Cleavage; by assemblin; Release site By similarity
Sitei610 – 6112Cleavage; by assemblin; Tail site By similarity

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. protein binding Source: IntAct
  3. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Viral capsid assembly, Virus exit from host cell

Protein family/group databases

MEROPSiS21.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid scaffolding protein
Alternative name(s):
Capsid protein P40
Protease precursor
Short name:
pPR
Virion structural protein UL26
Cleaved into the following 2 chains:
Alternative name(s):
Capsid protein VP24
Protease
Alternative name(s):
Capsid protein VP22A
Gene namesi
Name:UL26
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635Capsid scaffolding proteinPRO_0000027244Add
BLAST
Chaini1 – 247247AssemblinPRO_0000027245Add
BLAST
Chaini248 – 635388Assembly proteinPRO_0000027246Add
BLAST

Post-translational modificationi

Capsid scaffolding protein is cleaved by assemblin after completion of icosahedral capsid formation. Cleavages occur at two or more sites: release and tail site By similarity.
Assembly protein is phosphorylated.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Capsid scaffolding protein homomultimerizes and interacts with major capsid protein VP5. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein VP5 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8621986,EBI-8621986
UL19P064913EBI-8621986,EBI-7608705

Protein-protein interaction databases

DIPiDIP-1096N.
IntActiP10210. 1 interaction.
MINTiMINT-6732542.

Structurei

3D structure databases

ProteinModelPortaliP10210.
SMRiP10210. Positions 17-247.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni324 – 34320Interaction with pAPAdd
BLAST
Regioni618 – 63518Interaction with major capsid proteinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi426 – 4294Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi514 – 59077Pro-richAdd
BLAST

Domaini

Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD) By similarity.

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.16.10. 1 hit.
InterProiIPR001847. Peptidase_S21.
[Graphical view]
PfamiPF00716. Peptidase_S21. 1 hit.
[Graphical view]
PRINTSiPR00236. HSVCAPSIDP40.
SUPFAMiSSF50789. SSF50789. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Isoform Capsid scaffolding protein (identifier: P10210-1) [UniParc]FASTAAdd to Basket

Also known as: pPR, UL26

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAADAPGDRM EEPLPDRAVP IYVAGFLALY DSGDSGELAL DPDTVRAALP    50
PDNPLPINVD HRAGCEVGRV LAVVDDPRGP FFVGLIACVQ LERVLETAAS 100
AAIFERRGPP LSREERLLYL ITNYLPSVSL ATKRLGGEAH PDRTLFAHVA 150
LCAIGRRLGT IVTYDTGLDA AIAPFRHLSP ASREGARRLA AEAELALSGR 200
TWAPGVEALT HTLLSTAVNN MMLRDRWSLV AERRRQAGIA GHTYLQASEK 250
FKMWGAEPVS APARGYKNGA PESTDIPPGS IAAAPQGDRC PIVRQRGVAL 300
SPVLPPMNPV PTSGTPAPAP PGDGSYLWIP ASHYNQLVAG HAAPQPQPHS 350
AFGFPAAAGS VAYGPHGAGL SQHYPPHVAH QYPGVLFSGP SPLEAQIAAL 400
VGAIAADRQA GGQPAAGDPG VRGSGKRRRY EAGPSESYCD QDEPDADYPY 450
YPGEARGAPR GVDSRRAARH SPGTNETITA LMGAVTSLQQ ELAHMRARTS 500
APYGMYTPVA HYRPQVGEPE PTTTHPALCP PEAVYRPPPH SAPYGPPQGP 550
ASHAPTPPYA PAACPPGPPP PPCPSTQTRA PLPTEPAFPP AATGSQPEAS 600
NAEAGALVNA SSAAHVDVDT ARAADLFVSQ MMGAR 635
Length:635
Mass (Da):66,471
Last modified:July 1, 1989 - v1
Checksum:iF3B3C7D42F3D062D
GO
Isoform pAP (identifier: P10210-2) [UniParc]FASTAAdd to Basket

Also known as: Assembly protein, UL26.5 protein

The sequence of this isoform differs from the canonical sequence as follows:
     1-306: Missing.

Show »
Length:329
Mass (Da):33,760
Checksum:iDF690388D0630F9D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 306306Missing in isoform pAP. VSP_037414Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32318.1.
X14112 Genomic DNA. Translation: CAA32319.1.
PIRiH30084. WMBEW6.
RefSeqiNP_044627.1. NC_001806.1.
NP_044628.1. NC_001806.1.

Genome annotation databases

GeneIDi2703453.
2703454.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32318.1 .
X14112 Genomic DNA. Translation: CAA32319.1 .
PIRi H30084. WMBEW6.
RefSeqi NP_044627.1. NC_001806.1.
NP_044628.1. NC_001806.1.

3D structure databases

ProteinModelPortali P10210.
SMRi P10210. Positions 17-247.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-1096N.
IntActi P10210. 1 interaction.
MINTi MINT-6732542.

Chemistry

BindingDBi P10210.
ChEMBLi CHEMBL3518.

Protein family/group databases

MEROPSi S21.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703453.
2703454.

Family and domain databases

Gene3Di 3.20.16.10. 1 hit.
InterProi IPR001847. Peptidase_S21.
[Graphical view ]
Pfami PF00716. Peptidase_S21. 1 hit.
[Graphical view ]
PRINTSi PR00236. HSVCAPSIDP40.
SUPFAMi SSF50789. SSF50789. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of genes encoding two capsid proteins (VP24 and VP26) of herpes simplex virus type 1."
    Davison M.D., Rixon F.J., Davison A.J.
    J. Gen. Virol. 73:2709-2713(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 11-29; 77-91 AND 223-241.

Entry informationi

Entry nameiSCAF_HHV11
AccessioniPrimary (citable) accession number: P10210
Secondary accession number(s): O09798
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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