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P10209 (UL25_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Virion-packaging protein UL25
Gene names
Name:UL25
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role at the late stage in the encapsidation of viral DNA, assuring correct genome cleavage and presumably stabilizing capsids that contain full-lengtht viral genomes. Located on the external vertices of the T=16 icosahedric capsid, may bind together the tegument and the capsid through interaction with large tegument protein UL36. Could mediate, via binding to host nucleoporin NUC214, the capsid docking to the nuclear pore allowing entry of the viral genome into the nucleus. Ref.6 Ref.7

Subunit structure

Heterodimerizes with UL17. Interacts with capsid proteins VP5 and VP19C, essentially at the pentamer vertices. May interact with UL36. Interacts with host NUP14; this interaction might be essential to the capsid docking to the host nuclear pore. Interacts with host C9orf69. Ref.2 Ref.4 Ref.5 Ref.8 Ref.9

Subcellular location

Virion. Host nucleus Ref.3.

Sequence similarities

Belongs to the herpesviridae UL25 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580Virion-packaging protein UL25
PRO_0000115993

Regions

Region1 – 5050Interaction with major capsid protein

Secondary structure

............................................................ 580
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10209 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 3F8F0B7C122B2E36

FASTA58062,670
        10         20         30         40         50         60 
MDPYCPFDAL DVWEHRRFIV ADSRNFITPE FPRDFWMSPV FNLPRETAAE QVVVLQAQRT 

        70         80         90        100        110        120 
AAAAALENAA MQAAELPVDI ERRLRPIERN VHEIAGALEA LETAAAAAEE ADAARGDEPA 

       130        140        150        160        170        180 
GGGDGGAPPG LAVAEMEVQI VRNDPPLRYD TNLPVDLLHM VYAGRGATGS SGVVFGTWYR 

       190        200        210        220        230        240 
TIQDRTITDF PLTTRSADFR DGRMSKTFMT ALVLSLQACG RLYVGQRHYS AFECAVLCLY 

       250        260        270        280        290        300 
LLYRNTHGAA DDSDRAPVTF GDLLGRLPRY LACLAAVIGT EGGRPQYRYR DDKLPKTQFA 

       310        320        330        340        350        360 
AGGGRYEHGA LASHIVIATL MHHGVLPAAP GDVPRDASTH VNPDGVAHHD DINRAAAAFL 

       370        380        390        400        410        420 
SRGHNLFLWE DQTLLRATAN TITALGVIQR LLANGNVYAD RLNNRLQLGM LIPGAVPSEA 

       430        440        450        460        470        480 
IARGASGSDS GAIKSGDNNL EALCANYVLP LYRADPAVEL TQLFPGLAAL CLDAQAGRPV 

       490        500        510        520        530        540 
GSTRRVVDMS SGARQAALVR LTALELINRT RTNPTPVGEV IHAHDALAIQ YEQGLGLLAQ 

       550        560        570        580 
QARIGLGSNT KRFSAFNVSS DYDMLYFLCL GFIPQYLSAV 

« Hide

References

[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Role of the UL25 gene product in packaging DNA into the herpes simplex virus capsid: location of UL25 product in the capsid and demonstration that it binds DNA."
Ogasawara M., Suzutani T., Yoshida I., Azuma M.
J. Virol. 75:1427-1436(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VP5 AND VP19C.
[3]"Herpes simplex virus capsid structure: DNA packaging protein UL25 is located on the external surface of the capsid near the vertices."
Newcomb W.W., Homa F.L., Brown J.C.
J. Virol. 80:6286-6294(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Allosteric signaling and a nuclear exit strategy: binding of UL25/UL17 heterodimers to DNA-Filled HSV-1 capsids."
Trus B.L., Newcomb W.W., Cheng N., Cardone G., Marekov L., Homa F.L., Brown J.C., Steven A.C.
Mol. Cell 26:479-489(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL17.
[5]"The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins."
Coller K.E., Lee J.I., Ueda A., Smith G.A.
J. Virol. 81:11790-11797(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL36.
[6]"The UL25 gene product of herpes simplex virus type 1 is involved in uncoating of the viral genome."
Preston V.G., Murray J., Preston C.M., McDougall I.M., Stow N.D.
J. Virol. 82:6654-6666(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Role of the UL25 protein in herpes simplex virus DNA encapsidation."
Cockrell S.K., Sanchez M.E., Erazo A., Homa F.L.
J. Virol. 83:47-57(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Herpesvirus capsid association with the nuclear pore complex and viral DNA release involve the nucleoporin CAN/Nup214 and the capsid protein pUL25."
Pasdeloup D., Blondel D., Isidro A.L., Rixon F.J.
J. Virol. 83:6610-6623(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN NUP214.
[9]"Host cell protein C9orf9 promotes viral proliferation via interaction with HSV-1 UL25 protein."
Zhang Y., Li Y.M., Liu L.D., Jiang L., Ji M., Jiang R.J., Guo L., Liao Y., Li Q.H.
Zhongguo Bing Du Xue 26:171-180(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN C9ORF69.
[10]"Structural characterization of the UL25 DNA-packaging protein from herpes simplex virus type 1."
Bowman B.R., Welschhans R.L., Jayaram H., Stow N.D., Preston V.G., Quiocho F.A.
J. Virol. 80:2309-2317(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 134-580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32317.1.
PIRWMBEW5. G30084.
RefSeqNP_044626.1. NC_001806.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2F5UX-ray2.10A134-580[»]
ProteinModelPortalP10209.
SMRP10209. Positions 134-577.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60387N.
IntActP10209. 6 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703377.

Family and domain databases

InterProIPR002493. Herpes_UL25.
[Graphical view]
PfamPF01499. Herpes_UL25. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10209.

Entry information

Entry nameUL25_HHV11
AccessionPrimary (citable) accession number: P10209
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references