SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P10209

- UL25_HHV11

UniProt

P10209 - UL25_HHV11

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Virion-packaging protein UL25

Gene
UL25
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role at the late stage in the encapsidation of viral DNA, assuring correct genome cleavage and presumably stabilizing capsids that contain full-lengtht viral genomes. Located on the external vertices of the T=16 icosahedric capsid, may bind together the tegument and the capsid through interaction with large tegument protein UL36. Could mediate, via binding to host nucleoporin NUC214, the capsid docking to the nuclear pore allowing entry of the viral genome into the nucleus.2 Publications

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral genome packaging Source: InterPro
  3. viral penetration into host nucleus Source: UniProtKB-KW
  4. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral genome packaging, Viral penetration into host nucleus, Virus entry into host cell, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Virion-packaging protein UL25
Gene namesi
Name:UL25
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

Virion. Host nucleus 1 Publication

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. intracellular Source: GOC
  3. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Virion-packaging protein UL25PRO_0000115993Add
BLAST

Interactioni

Subunit structurei

Heterodimerizes with UL17. Interacts with capsid proteins VP5 and VP19C, essentially at the pentamer vertices. May interact with UL36. Interacts with host NUP14; this interaction might be essential to the capsid docking to the host nuclear pore. Interacts with host C9orf69.5 Publications

Protein-protein interaction databases

DIPiDIP-60387N.
IntActiP10209. 6 interactions.

Structurei

Secondary structure

1
580
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi136 – 1383
Beta strandi147 – 1515
Helixi153 – 16311
Helixi165 – 1673
Beta strandi168 – 1703
Helixi177 – 18913
Helixi191 – 1955
Helixi200 – 2023
Helixi206 – 21813
Helixi231 – 24616
Helixi260 – 2656
Helixi267 – 27812
Helixi291 – 2933
Turni308 – 3136
Helixi315 – 3228
Helixi351 – 36010
Helixi368 – 3703
Helixi372 – 39221
Helixi401 – 4033
Helixi408 – 4114
Helixi438 – 4469
Helixi448 – 4547
Helixi460 – 4634
Helixi465 – 47410
Helixi491 – 4944
Helixi496 – 50813
Helixi517 – 54024
Helixi547 – 5493
Helixi550 – 5545
Turni555 – 5573
Helixi561 – 57010

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F5UX-ray2.10A134-580[»]
ProteinModelPortaliP10209.
SMRiP10209. Positions 134-577.

Miscellaneous databases

EvolutionaryTraceiP10209.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5050Interaction with major capsid proteinAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002493. Herpes_UL25.
[Graphical view]
PfamiPF01499. Herpes_UL25. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10209-1 [UniParc]FASTAAdd to Basket

« Hide

MDPYCPFDAL DVWEHRRFIV ADSRNFITPE FPRDFWMSPV FNLPRETAAE    50
QVVVLQAQRT AAAAALENAA MQAAELPVDI ERRLRPIERN VHEIAGALEA 100
LETAAAAAEE ADAARGDEPA GGGDGGAPPG LAVAEMEVQI VRNDPPLRYD 150
TNLPVDLLHM VYAGRGATGS SGVVFGTWYR TIQDRTITDF PLTTRSADFR 200
DGRMSKTFMT ALVLSLQACG RLYVGQRHYS AFECAVLCLY LLYRNTHGAA 250
DDSDRAPVTF GDLLGRLPRY LACLAAVIGT EGGRPQYRYR DDKLPKTQFA 300
AGGGRYEHGA LASHIVIATL MHHGVLPAAP GDVPRDASTH VNPDGVAHHD 350
DINRAAAAFL SRGHNLFLWE DQTLLRATAN TITALGVIQR LLANGNVYAD 400
RLNNRLQLGM LIPGAVPSEA IARGASGSDS GAIKSGDNNL EALCANYVLP 450
LYRADPAVEL TQLFPGLAAL CLDAQAGRPV GSTRRVVDMS SGARQAALVR 500
LTALELINRT RTNPTPVGEV IHAHDALAIQ YEQGLGLLAQ QARIGLGSNT 550
KRFSAFNVSS DYDMLYFLCL GFIPQYLSAV 580
Length:580
Mass (Da):62,670
Last modified:July 1, 1989 - v1
Checksum:i3F8F0B7C122B2E36
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32317.1.
PIRiG30084. WMBEW5.
RefSeqiNP_044626.1. NC_001806.1.

Genome annotation databases

GeneIDi2703377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32317.1 .
PIRi G30084. WMBEW5.
RefSeqi NP_044626.1. NC_001806.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2F5U X-ray 2.10 A 134-580 [» ]
ProteinModelPortali P10209.
SMRi P10209. Positions 134-577.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60387N.
IntActi P10209. 6 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703377.

Miscellaneous databases

EvolutionaryTracei P10209.

Family and domain databases

InterProi IPR002493. Herpes_UL25.
[Graphical view ]
Pfami PF01499. Herpes_UL25. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Role of the UL25 gene product in packaging DNA into the herpes simplex virus capsid: location of UL25 product in the capsid and demonstration that it binds DNA."
    Ogasawara M., Suzutani T., Yoshida I., Azuma M.
    J. Virol. 75:1427-1436(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VP5 AND VP19C.
  3. "Herpes simplex virus capsid structure: DNA packaging protein UL25 is located on the external surface of the capsid near the vertices."
    Newcomb W.W., Homa F.L., Brown J.C.
    J. Virol. 80:6286-6294(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Allosteric signaling and a nuclear exit strategy: binding of UL25/UL17 heterodimers to DNA-Filled HSV-1 capsids."
    Trus B.L., Newcomb W.W., Cheng N., Cardone G., Marekov L., Homa F.L., Brown J.C., Steven A.C.
    Mol. Cell 26:479-489(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL17.
  5. "The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins."
    Coller K.E., Lee J.I., Ueda A., Smith G.A.
    J. Virol. 81:11790-11797(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL36.
  6. "The UL25 gene product of herpes simplex virus type 1 is involved in uncoating of the viral genome."
    Preston V.G., Murray J., Preston C.M., McDougall I.M., Stow N.D.
    J. Virol. 82:6654-6666(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Role of the UL25 protein in herpes simplex virus DNA encapsidation."
    Cockrell S.K., Sanchez M.E., Erazo A., Homa F.L.
    J. Virol. 83:47-57(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Herpesvirus capsid association with the nuclear pore complex and viral DNA release involve the nucleoporin CAN/Nup214 and the capsid protein pUL25."
    Pasdeloup D., Blondel D., Isidro A.L., Rixon F.J.
    J. Virol. 83:6610-6623(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN NUP214.
  9. "Host cell protein C9orf9 promotes viral proliferation via interaction with HSV-1 UL25 protein."
    Zhang Y., Li Y.M., Liu L.D., Jiang L., Ji M., Jiang R.J., Guo L., Liao Y., Li Q.H.
    Zhongguo Bing Du Xue 26:171-180(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN C9ORF69.
  10. "Structural characterization of the UL25 DNA-packaging protein from herpes simplex virus type 1."
    Bowman B.R., Welschhans R.L., Jayaram H., Stow N.D., Preston V.G., Quiocho F.A.
    J. Virol. 80:2309-2317(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 134-580.

Entry informationi

Entry nameiUL25_HHV11
AccessioniPrimary (citable) accession number: P10209
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi