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P10209

- UL25_HHV11

UniProt

P10209 - UL25_HHV11

Protein

Virion-packaging protein UL25

Gene

UL25

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Plays a role at the late stage in the encapsidation of viral DNA, assuring correct genome cleavage and presumably stabilizing capsids that contain full-lengtht viral genomes. Located on the external vertices of the T=16 icosahedric capsid, may bind together the tegument and the capsid through interaction with large tegument protein UL36. Could mediate, via binding to host nucleoporin NUC214, the capsid docking to the nuclear pore allowing entry of the viral genome into the nucleus.2 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral genome packaging Source: InterPro
    3. viral penetration into host nucleus Source: UniProtKB-KW
    4. viral release from host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral genome packaging, Viral penetration into host nucleus, Virus entry into host cell, Virus exit from host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Virion-packaging protein UL25
    Gene namesi
    Name:UL25
    OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10299 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009294: Genome

    Subcellular locationi

    Virion 1 Publication. Host nucleus 1 Publication

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. intracellular Source: GOC
    3. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 580580Virion-packaging protein UL25PRO_0000115993Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimerizes with UL17. Interacts with capsid proteins VP5 and VP19C, essentially at the pentamer vertices. May interact with UL36. Interacts with host NUP14; this interaction might be essential to the capsid docking to the host nuclear pore. Interacts with host C9orf69.5 Publications

    Protein-protein interaction databases

    DIPiDIP-60387N.
    IntActiP10209. 6 interactions.

    Structurei

    Secondary structure

    1
    580
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi136 – 1383
    Beta strandi147 – 1515
    Helixi153 – 16311
    Helixi165 – 1673
    Beta strandi168 – 1703
    Helixi177 – 18913
    Helixi191 – 1955
    Helixi200 – 2023
    Helixi206 – 21813
    Helixi231 – 24616
    Helixi260 – 2656
    Helixi267 – 27812
    Helixi291 – 2933
    Turni308 – 3136
    Helixi315 – 3228
    Helixi351 – 36010
    Helixi368 – 3703
    Helixi372 – 39221
    Helixi401 – 4033
    Helixi408 – 4114
    Helixi438 – 4469
    Helixi448 – 4547
    Helixi460 – 4634
    Helixi465 – 47410
    Helixi491 – 4944
    Helixi496 – 50813
    Helixi517 – 54024
    Helixi547 – 5493
    Helixi550 – 5545
    Turni555 – 5573
    Helixi561 – 57010

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2F5UX-ray2.10A134-580[»]
    ProteinModelPortaliP10209.
    SMRiP10209. Positions 134-577.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10209.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 5050Interaction with major capsid proteinAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae UL25 family.Curated

    Family and domain databases

    InterProiIPR002493. Herpes_UL25.
    [Graphical view]
    PfamiPF01499. Herpes_UL25. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10209-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPYCPFDAL DVWEHRRFIV ADSRNFITPE FPRDFWMSPV FNLPRETAAE    50
    QVVVLQAQRT AAAAALENAA MQAAELPVDI ERRLRPIERN VHEIAGALEA 100
    LETAAAAAEE ADAARGDEPA GGGDGGAPPG LAVAEMEVQI VRNDPPLRYD 150
    TNLPVDLLHM VYAGRGATGS SGVVFGTWYR TIQDRTITDF PLTTRSADFR 200
    DGRMSKTFMT ALVLSLQACG RLYVGQRHYS AFECAVLCLY LLYRNTHGAA 250
    DDSDRAPVTF GDLLGRLPRY LACLAAVIGT EGGRPQYRYR DDKLPKTQFA 300
    AGGGRYEHGA LASHIVIATL MHHGVLPAAP GDVPRDASTH VNPDGVAHHD 350
    DINRAAAAFL SRGHNLFLWE DQTLLRATAN TITALGVIQR LLANGNVYAD 400
    RLNNRLQLGM LIPGAVPSEA IARGASGSDS GAIKSGDNNL EALCANYVLP 450
    LYRADPAVEL TQLFPGLAAL CLDAQAGRPV GSTRRVVDMS SGARQAALVR 500
    LTALELINRT RTNPTPVGEV IHAHDALAIQ YEQGLGLLAQ QARIGLGSNT 550
    KRFSAFNVSS DYDMLYFLCL GFIPQYLSAV 580
    Length:580
    Mass (Da):62,670
    Last modified:July 1, 1989 - v1
    Checksum:i3F8F0B7C122B2E36
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32317.1.
    PIRiG30084. WMBEW5.
    RefSeqiNP_044626.1. NC_001806.1.

    Genome annotation databases

    GeneIDi2703377.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32317.1 .
    PIRi G30084. WMBEW5.
    RefSeqi NP_044626.1. NC_001806.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2F5U X-ray 2.10 A 134-580 [» ]
    ProteinModelPortali P10209.
    SMRi P10209. Positions 134-577.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60387N.
    IntActi P10209. 6 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703377.

    Miscellaneous databases

    EvolutionaryTracei P10209.

    Family and domain databases

    InterProi IPR002493. Herpes_UL25.
    [Graphical view ]
    Pfami PF01499. Herpes_UL25. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
      J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Role of the UL25 gene product in packaging DNA into the herpes simplex virus capsid: location of UL25 product in the capsid and demonstration that it binds DNA."
      Ogasawara M., Suzutani T., Yoshida I., Azuma M.
      J. Virol. 75:1427-1436(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VP5 AND VP19C.
    3. "Herpes simplex virus capsid structure: DNA packaging protein UL25 is located on the external surface of the capsid near the vertices."
      Newcomb W.W., Homa F.L., Brown J.C.
      J. Virol. 80:6286-6294(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Allosteric signaling and a nuclear exit strategy: binding of UL25/UL17 heterodimers to DNA-Filled HSV-1 capsids."
      Trus B.L., Newcomb W.W., Cheng N., Cardone G., Marekov L., Homa F.L., Brown J.C., Steven A.C.
      Mol. Cell 26:479-489(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UL17.
    5. "The capsid and tegument of the alphaherpesviruses are linked by an interaction between the UL25 and VP1/2 proteins."
      Coller K.E., Lee J.I., Ueda A., Smith G.A.
      J. Virol. 81:11790-11797(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UL36.
    6. "The UL25 gene product of herpes simplex virus type 1 is involved in uncoating of the viral genome."
      Preston V.G., Murray J., Preston C.M., McDougall I.M., Stow N.D.
      J. Virol. 82:6654-6666(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Role of the UL25 protein in herpes simplex virus DNA encapsidation."
      Cockrell S.K., Sanchez M.E., Erazo A., Homa F.L.
      J. Virol. 83:47-57(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Herpesvirus capsid association with the nuclear pore complex and viral DNA release involve the nucleoporin CAN/Nup214 and the capsid protein pUL25."
      Pasdeloup D., Blondel D., Isidro A.L., Rixon F.J.
      J. Virol. 83:6610-6623(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN NUP214.
    9. "Host cell protein C9orf9 promotes viral proliferation via interaction with HSV-1 UL25 protein."
      Zhang Y., Li Y.M., Liu L.D., Jiang L., Ji M., Jiang R.J., Guo L., Liao Y., Li Q.H.
      Zhongguo Bing Du Xue 26:171-180(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN C9ORF69.
    10. "Structural characterization of the UL25 DNA-packaging protein from herpes simplex virus type 1."
      Bowman B.R., Welschhans R.L., Jayaram H., Stow N.D., Preston V.G., Quiocho F.A.
      J. Virol. 80:2309-2317(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 134-580.

    Entry informationi

    Entry nameiUL25_HHV11
    AccessioniPrimary (citable) accession number: P10209
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3