P10200 (UL16_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Capsid-binding protein UL16

Gene names

Name:

UL16

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in virion morphogenesis and budding by interacting with the capsid. Interacts also in the virion with the membrane bound tegument protein UL11. Upon virion binding to target cell, a signaling is triggered through UL11 to disrupt UL16-capsid interaction, thereby preparing capsid uncoating. Ref.4
Subunit structure	Interacts with UL11 and with capsid proteins. Ref.2 Ref.3 Ref.5
Subcellular location	Virion tegument Potential. Host cytoplasm. Host nucleus. Note: Found in the nucleus up to 18 hours postinfection, but at later times is localized to punctate, cytoplasmic structures. Ref.3
Sequence similarities	Belongs to the herpesviridae HHV-1 UL16 family.

Ontologies

Keywords
Cellular component	Host cytoplasm Host nucleus Virion Virion tegument
Developmental stage	Late protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral tegument Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Capsid-binding protein UL16		PRO_0000115949
Experimental info
	Mutagenesis	221	1	C → S: No effect on the interaction with UL11. Ref.5
	Mutagenesis	244	1	C → S: No effect on the interaction with UL11. Ref.5
	Mutagenesis	247	1	C → S: Complete loss of interaction with UL11. Ref.5
	Mutagenesis	269	1	C → S: Complete loss of interaction with UL11. Ref.5
	Mutagenesis	271	1	C → S: Complete loss of interaction with UL11. Ref.5
	Mutagenesis	275	1	C → S: Complete loss of interaction with UL11. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P10200 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 85D9914C533FE34B	FASTA	373	40,443
	10 20 30 40 50 60 MAQLGPRRPL APPGPPGTLP RPDSRAGARG TRDRVDDLGT DVDSIARIVN SVFVWRVVRA 70 80 90 100 110 120 DERLKIFRCL TVLTEPLCQV ALPNPDPGRA LFCEIFLYLT RPKALRLPPN TFFALFFFNR 130 140 150 160 170 180 ERRYCAIVHL RSVTHPLTPL LCTLTFARIR AATPPEETPD PTTEQLAEEP VVGELDGAYL 190 200 210 220 230 240 VPAKTPPEPG ACCALGPGAW WHLPSGQIYC WAMDSDLGSL CPPGSRARHL GWLLARITNH 250 260 270 280 290 300 PGGCESCAPP PHIDSANALW LSSVVTESCP CVAPCLWAKM AQCTLAVQGD ASLCPLLFGH 310 320 330 340 350 360 PVDTVTLLQA PRRPCITDRL QEVVGGRCGA DNIPPTSAGW RLCVFSSYIS RLFATSCPTV 370 ARAVARASSS DPE

References

[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Binding partners for the UL11 tegument protein of herpes simplex virus type 1." Loomis J.S., Courtney R.J., Wills J.W. J. Virol. 77:11417-11424(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL11. Strain: KOS.
[3]	"Dynamic interactions of the UL16 tegument protein with the capsid of herpes simplex virus." Meckes D.G. Jr., Wills J.W. J. Virol. 81:13028-13036(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CAPSID, SUBCELLULAR LOCATION. Strain: KOS.
[4]	"Structural rearrangement within an enveloped virus upon binding to the host cell." Meckes D.G. Jr., Wills J.W. J. Virol. 82:10429-10435(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: KOS.
[5]	"Analysis of the interaction between the UL11 and UL16 tegument proteins of herpes simplex virus." Yeh P.-C., Meckes D.G. Jr., Wills J.W. J. Virol. 82:10693-10700(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL11, MUTAGENESIS OF CYS-221; CYS-244; CYS-247; CYS-269; CYS-271 AND CYS-275. Strain: KOS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32328.1.
PIR	WMBET6. G30083.
RefSeq	NP_044617.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6732685.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703386.
Phylogenomic databases
ProtClustDB	PHA3349.
Family and domain databases
InterPro	IPR004286. Herpes_UL16/UL94. [Graphical view]
Pfam	PF03044. Herpes_UL16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UL16_HHV11

Accession

Primary (citable) accession number: P10200

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program