P10193 (OBP_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication origin-binding protein
Short name=OBP

Alternative name(s):
OriBP

Gene names

ORF Names:

UL9

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a docking protein to recruit essential components of the viral replication machinery to viral DNA origins. In the presence of the major DNA-binding protein, opens dsDNA leading to a conformational change in the origin that facilitates DNA unwinding and subsequent replication. Ref.6
Subunit structure	Homodimer. Interacts with the major DNA-binding protein ICP8. Interacts with the helicase/primase component UL8 and the polymerase accessory protein UL42. Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Host nucleus Potential.
Sequence similarities	Belongs to the herpesviridae OriBP family. Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Host nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 851	851	Replication origin-binding protein		PRO_0000115866
Regions
	Domain	68 – 233	166	Helicase ATP-binding
	Nucleotide binding	81 – 88	8	ATP Potential
Natural variations
	Natural variant	149	1	R → Q in strain: Nonneuroinvasive mutant HF10.
	Natural variant	204	1	I → T in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
	Natural variant	280	1	E → D in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
	Natural variant	668 – 669	2	GP → SH in strain: Nonneuroinvasive mutant HF10.
	Natural variant	745	1	A → T in strain: Nonneuroinvasive mutant HF10.
	Natural variant	797	1	A → V in strain: Nonneuroinvasive mutant HF10.
	Natural variant	818	1	S → N in strain: 17 syn+.

Sequences

	Sequence		Length	Mass (Da)
	P10193 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 961A133FE7A30CA7	FASTA	851	94,262
	10 20 30 40 50 60 MPFVGGAESG DPLGAGRPIG DDECEQYTSS VSLARMLYGG DLAEWVPRVH PKTTIERQQH 70 80 90 100 110 120 GPVTFPNASA PTARCVTVVR APMGSGKTTA LIRWLREAIH SPDTSVLVVS CRRSFTQTLA 130 140 150 160 170 180 TRFAESGLVD FVTYFSSTNY IMNDRPFHRL IVQVESLHRV GPNLLNNYDV LVLDEVMSTL 190 200 210 220 230 240 GQLYSPTMQQ LGRVDALMLR LLRICPRIIA MDATANAQLV DFLCGLRGEK NVHVVVGEYA 250 260 270 280 290 300 MPGFSARRCL FLPRLGTELL QAALRPPGPP SGPSPDASPE ARGATFFGEL EARLGGGDNI 310 320 330 340 350 360 CIFSSTVSFA EIVARFCRQF TDRVLLLHSL TPLGDVTTWG QYRVVIYTTV VTVGLSFDPL 370 380 390 400 410 420 HFDGMFAYVK PMNYGPDMVS VYQSLGRVRT LRKGELLIYM DGSGARSEPV FTPMLLNHVV 430 440 450 460 470 480 SSCGQWPAQF SQVTNLLCRR FKGRCDASAC DTSLGRGSRI YNKFRYKHYF ERCTLACLSD 490 500 510 520 530 540 SLNILHMLLT LNCIRVRFWG HDDTLTPKDF CLFLRGVHFD ALRAQRDLRE LRCRDPEASL 550 560 570 580 590 600 PAQAAETEEV GLFVEKYLRS DVAPAEIVAL MRNLNSLMGR TRFIYLALLE ACLRVPMATR 610 620 630 640 650 660 SSAIFRRIYD HYATGVIPTI NVTGELELVA LPPTLNVTPV WELLCLCSTM AARLHWDSAA 670 680 690 700 710 720 GGSGRTFGPD DVLDLLTPHY DRYMQLVFEL GHCNVTDGLL LSEEAVKRVA DALSGCPPRG 730 740 750 760 770 780 SVSETDHAVA LFKIIWGELF GVQMAKSTQT FPGAGRVKNL TKQTIVGLLD AHHIDHSACR 790 800 810 820 830 840 THRQLYALLM AHKREFAGAR FKLRVPAWGR CLRTHSSSAN PNADIILEAA LSELPTEAWP 850 MMQGAVNFST L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed: 2839594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Structures of herpes simplex virus type 1 genes required for replication of virus DNA." McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D. J. Virol. 62:444-453(1988) [PubMed: 2826807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus." Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y. Microbes Infect. 9:142-149(2007) [PubMed: 17218138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nonneuroinvasive mutant HF10.
[4]	"Herpes simplex virus type 1 bacterial artificial chromosome." Cunningham C., Davison A.J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 17 syn+.
[5]	"The herpes simplex virus type 1 origin-binding protein interacts specifically with the viral UL8 protein." McLean G.W., Abbotts A.P., Parry M.E., Marsden H.S., Stow N.D. J. Gen. Virol. 75:2699-2706(1994) [PubMed: 7931156] [Abstract] Cited for: INTERACTION WITH UL8.
[6]	"Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo." Boehmer P.E., Craigie M.C., Stow N.D., Lehman I.R. J. Biol. Chem. 269:29329-29334(1994) [PubMed: 7961904] [Abstract] Cited for: INTERACTION WITH ICP8, FUNCTION.
[7]	"Interaction between the herpes simplex virus type 1 origin-binding and DNA polymerase accessory proteins." Monahan S.J., Grinstead L.A., Olivieri W., Parris D.S. Virology 241:122-130(1998) [PubMed: 9454723] [Abstract] Cited for: INTERACTION WITH UL42.
[8]	"Direct interaction between the N- and C-terminal portions of the herpes simplex virus type 1 origin binding protein UL9 implies the formation of a head-to-tail dimer." Chattopadhyay S., Weller S.K. J. Virol. 81:13659-13667(2007) [PubMed: 17942532] [Abstract] Cited for: SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32345.1. M19120 Genomic DNA. Translation: AAA45822.1. DQ889502 Genomic DNA. Translation: ABI63471.1. FJ593289 Genomic DNA. Translation: ACM62231.1.
PIR	WMBEU9. B29890.
RefSeq	NP_044610.1. NC_001806.1.
3D structure databases
ProteinModelPortal	P10193.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1095N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703434.
Phylogenomic databases
ProtClustDB	CLSP2509628.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR003450. Replication_origin-bd. [Graphical view]
Pfam	PF02399. Herpes_ori_bp. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OBP_HHV11

Accession

Primary (citable) accession number: P10193
Secondary accession number(s): B9VQD6, Q09IC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	September 21, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program