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P10193 (OBP_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication origin-binding protein

Short name=OBP
Alternative name(s):
OriBP
Gene names
ORF Names:UL9
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length851 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a docking protein to recruit essential components of the viral replication machinery to viral DNA origins. In the presence of the major DNA-binding protein, opens dsDNA leading to a conformational change in the origin that facilitates DNA unwinding and subsequent replication. Ref.6

Subunit structure

Homodimer. Interacts with the major DNA-binding protein ICP8. Interacts with the helicase/primase component UL8 and the polymerase accessory protein UL42. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Host nucleus Potential.

Sequence similarities

Belongs to the herpesviridae OriBP family.

Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentHost nucleus
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication origin binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

POLA1P098844EBI-8596799,EBI-850026From a different organism.
UL42P102263EBI-8596799,EBI-1029310

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 851851Replication origin-binding protein
PRO_0000115866

Regions

Domain68 – 233166Helicase ATP-binding
Nucleotide binding81 – 888ATP Potential

Natural variations

Natural variant1491R → Q in strain: Nonneuroinvasive mutant HF10.
Natural variant2041I → T in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural variant2801E → D in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural variant668 – 6692GP → SH in strain: Nonneuroinvasive mutant HF10.
Natural variant7451A → T in strain: Nonneuroinvasive mutant HF10.
Natural variant7971A → V in strain: Nonneuroinvasive mutant HF10.
Natural variant8181S → N in strain: 17 syn+.

Sequences

Sequence LengthMass (Da)Tools
P10193 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 961A133FE7A30CA7

FASTA85194,262
        10         20         30         40         50         60 
MPFVGGAESG DPLGAGRPIG DDECEQYTSS VSLARMLYGG DLAEWVPRVH PKTTIERQQH 

        70         80         90        100        110        120 
GPVTFPNASA PTARCVTVVR APMGSGKTTA LIRWLREAIH SPDTSVLVVS CRRSFTQTLA 

       130        140        150        160        170        180 
TRFAESGLVD FVTYFSSTNY IMNDRPFHRL IVQVESLHRV GPNLLNNYDV LVLDEVMSTL 

       190        200        210        220        230        240 
GQLYSPTMQQ LGRVDALMLR LLRICPRIIA MDATANAQLV DFLCGLRGEK NVHVVVGEYA 

       250        260        270        280        290        300 
MPGFSARRCL FLPRLGTELL QAALRPPGPP SGPSPDASPE ARGATFFGEL EARLGGGDNI 

       310        320        330        340        350        360 
CIFSSTVSFA EIVARFCRQF TDRVLLLHSL TPLGDVTTWG QYRVVIYTTV VTVGLSFDPL 

       370        380        390        400        410        420 
HFDGMFAYVK PMNYGPDMVS VYQSLGRVRT LRKGELLIYM DGSGARSEPV FTPMLLNHVV 

       430        440        450        460        470        480 
SSCGQWPAQF SQVTNLLCRR FKGRCDASAC DTSLGRGSRI YNKFRYKHYF ERCTLACLSD 

       490        500        510        520        530        540 
SLNILHMLLT LNCIRVRFWG HDDTLTPKDF CLFLRGVHFD ALRAQRDLRE LRCRDPEASL 

       550        560        570        580        590        600 
PAQAAETEEV GLFVEKYLRS DVAPAEIVAL MRNLNSLMGR TRFIYLALLE ACLRVPMATR 

       610        620        630        640        650        660 
SSAIFRRIYD HYATGVIPTI NVTGELELVA LPPTLNVTPV WELLCLCSTM AARLHWDSAA 

       670        680        690        700        710        720 
GGSGRTFGPD DVLDLLTPHY DRYMQLVFEL GHCNVTDGLL LSEEAVKRVA DALSGCPPRG 

       730        740        750        760        770        780 
SVSETDHAVA LFKIIWGELF GVQMAKSTQT FPGAGRVKNL TKQTIVGLLD AHHIDHSACR 

       790        800        810        820        830        840 
THRQLYALLM AHKREFAGAR FKLRVPAWGR CLRTHSSSAN PNADIILEAA LSELPTEAWP 

       850 
MMQGAVNFST L 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Structures of herpes simplex virus type 1 genes required for replication of virus DNA."
McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D.
J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nonneuroinvasive mutant HF10.
[4]"Herpes simplex virus type 1 bacterial artificial chromosome."
Cunningham C., Davison A.J.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 17 syn+.
[5]"The herpes simplex virus type 1 origin-binding protein interacts specifically with the viral UL8 protein."
McLean G.W., Abbotts A.P., Parry M.E., Marsden H.S., Stow N.D.
J. Gen. Virol. 75:2699-2706(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL8.
[6]"Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo."
Boehmer P.E., Craigie M.C., Stow N.D., Lehman I.R.
J. Biol. Chem. 269:29329-29334(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ICP8, FUNCTION.
[7]"Interaction between the herpes simplex virus type 1 origin-binding and DNA polymerase accessory proteins."
Monahan S.J., Grinstead L.A., Olivieri W., Parris D.S.
Virology 241:122-130(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL42.
[8]"Direct interaction between the N- and C-terminal portions of the herpes simplex virus type 1 origin binding protein UL9 implies the formation of a head-to-tail dimer."
Chattopadhyay S., Weller S.K.
J. Virol. 81:13659-13667(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32345.1.
M19120 Genomic DNA. Translation: AAA45822.1.
DQ889502 Genomic DNA. Translation: ABI63471.1.
FJ593289 Genomic DNA. Translation: ACM62231.1.
PIRWMBEU9. B29890.
RefSeqNP_044610.1. NC_001806.1.

3D structure databases

ProteinModelPortalP10193.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid971457. 1 interaction.
DIPDIP-1095N.
IntActP10193. 2 interactions.
MINTMINT-191327.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703434.

Phylogenomic databases

ProtClustDBCLSP2509628.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR003450. Replication_origin-bd.
[Graphical view]
PfamPF02399. Herpes_ori_bp. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOBP_HHV11
AccessionPrimary (citable) accession number: P10193
Secondary accession number(s): B9VQD6, Q09IC4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families