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P10193

- OBP_HHV11

UniProt

P10193 - OBP_HHV11

Protein

Replication origin-binding protein

Gene

UL9

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Functions as a docking protein to recruit essential components of the viral replication machinery to viral DNA origins. In the presence of the major DNA-binding protein, opens dsDNA leading to a conformational change in the origin that facilitates DNA unwinding and subsequent replication.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi81 – 888ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA replication origin binding Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication origin-binding protein
    Short name:
    OBP
    Alternative name(s):
    OriBP
    Gene namesi
    ORF Names:UL9
    OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10299 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009294: Genome

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 851851Replication origin-binding proteinPRO_0000115866Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Interacts with the major DNA-binding protein ICP8. Interacts with the helicase/primase component UL8 and the polymerase accessory protein UL42.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    POLA1P098844EBI-8596799,EBI-850026From a different organism.
    UL42P102263EBI-8596799,EBI-1029310

    Protein-protein interaction databases

    BioGridi971457. 1 interaction.
    DIPiDIP-1095N.
    IntActiP10193. 2 interactions.
    MINTiMINT-191327.

    Structurei

    3D structure databases

    ProteinModelPortaliP10193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini68 – 233166Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae OriBP family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR003450. Replication_origin-bd.
    [Graphical view]
    PfamiPF02399. Herpes_ori_bp. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10193-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPFVGGAESG DPLGAGRPIG DDECEQYTSS VSLARMLYGG DLAEWVPRVH    50
    PKTTIERQQH GPVTFPNASA PTARCVTVVR APMGSGKTTA LIRWLREAIH 100
    SPDTSVLVVS CRRSFTQTLA TRFAESGLVD FVTYFSSTNY IMNDRPFHRL 150
    IVQVESLHRV GPNLLNNYDV LVLDEVMSTL GQLYSPTMQQ LGRVDALMLR 200
    LLRICPRIIA MDATANAQLV DFLCGLRGEK NVHVVVGEYA MPGFSARRCL 250
    FLPRLGTELL QAALRPPGPP SGPSPDASPE ARGATFFGEL EARLGGGDNI 300
    CIFSSTVSFA EIVARFCRQF TDRVLLLHSL TPLGDVTTWG QYRVVIYTTV 350
    VTVGLSFDPL HFDGMFAYVK PMNYGPDMVS VYQSLGRVRT LRKGELLIYM 400
    DGSGARSEPV FTPMLLNHVV SSCGQWPAQF SQVTNLLCRR FKGRCDASAC 450
    DTSLGRGSRI YNKFRYKHYF ERCTLACLSD SLNILHMLLT LNCIRVRFWG 500
    HDDTLTPKDF CLFLRGVHFD ALRAQRDLRE LRCRDPEASL PAQAAETEEV 550
    GLFVEKYLRS DVAPAEIVAL MRNLNSLMGR TRFIYLALLE ACLRVPMATR 600
    SSAIFRRIYD HYATGVIPTI NVTGELELVA LPPTLNVTPV WELLCLCSTM 650
    AARLHWDSAA GGSGRTFGPD DVLDLLTPHY DRYMQLVFEL GHCNVTDGLL 700
    LSEEAVKRVA DALSGCPPRG SVSETDHAVA LFKIIWGELF GVQMAKSTQT 750
    FPGAGRVKNL TKQTIVGLLD AHHIDHSACR THRQLYALLM AHKREFAGAR 800
    FKLRVPAWGR CLRTHSSSAN PNADIILEAA LSELPTEAWP MMQGAVNFST 850
    L 851
    Length:851
    Mass (Da):94,262
    Last modified:July 1, 1989 - v1
    Checksum:i961A133FE7A30CA7
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti149 – 1491R → Q in strain: Nonneuroinvasive mutant HF10.
    Natural varianti204 – 2041I → T in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
    Natural varianti280 – 2801E → D in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
    Natural varianti668 – 6692GP → SH in strain: Nonneuroinvasive mutant HF10.
    Natural varianti745 – 7451A → T in strain: Nonneuroinvasive mutant HF10.
    Natural varianti797 – 7971A → V in strain: Nonneuroinvasive mutant HF10.
    Natural varianti818 – 8181S → N in strain: 17 syn+.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32345.1.
    M19120 Genomic DNA. Translation: AAA45822.1.
    DQ889502 Genomic DNA. Translation: ABI63471.1.
    FJ593289 Genomic DNA. Translation: ACM62231.1.
    PIRiB29890. WMBEU9.
    RefSeqiNP_044610.1. NC_001806.1.

    Genome annotation databases

    GeneIDi2703434.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32345.1 .
    M19120 Genomic DNA. Translation: AAA45822.1 .
    DQ889502 Genomic DNA. Translation: ABI63471.1 .
    FJ593289 Genomic DNA. Translation: ACM62231.1 .
    PIRi B29890. WMBEU9.
    RefSeqi NP_044610.1. NC_001806.1.

    3D structure databases

    ProteinModelPortali P10193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 971457. 1 interaction.
    DIPi DIP-1095N.
    IntActi P10193. 2 interactions.
    MINTi MINT-191327.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703434.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR003450. Replication_origin-bd.
    [Graphical view ]
    Pfami PF02399. Herpes_ori_bp. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
      J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Structures of herpes simplex virus type 1 genes required for replication of virus DNA."
      McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D.
      J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
      Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
      Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nonneuroinvasive mutant HF10.
    4. "Herpes simplex virus type 1 bacterial artificial chromosome."
      Cunningham C., Davison A.J.
      Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 17 syn+.
    5. "The herpes simplex virus type 1 origin-binding protein interacts specifically with the viral UL8 protein."
      McLean G.W., Abbotts A.P., Parry M.E., Marsden H.S., Stow N.D.
      J. Gen. Virol. 75:2699-2706(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UL8.
    6. "Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo."
      Boehmer P.E., Craigie M.C., Stow N.D., Lehman I.R.
      J. Biol. Chem. 269:29329-29334(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ICP8, FUNCTION.
    7. "Interaction between the herpes simplex virus type 1 origin-binding and DNA polymerase accessory proteins."
      Monahan S.J., Grinstead L.A., Olivieri W., Parris D.S.
      Virology 241:122-130(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UL42.
    8. "Direct interaction between the N- and C-terminal portions of the herpes simplex virus type 1 origin binding protein UL9 implies the formation of a head-to-tail dimer."
      Chattopadhyay S., Weller S.K.
      J. Virol. 81:13659-13667(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiOBP_HHV11
    AccessioniPrimary (citable) accession number: P10193
    Secondary accession number(s): B9VQD6, Q09IC4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3