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P10192 (HEPA_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA helicase/primase complex-associated protein

Short name=HEPA
Alternative name(s):
Primase-associated factor
Gene names
ORF Names:UL8
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length750 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The primase-associated factor has no known catalytic activity in the complex and may serve to facilitate the formation of the replisome by directly interacting with the origin-binding protein and the polymerase. Ref.5 Ref.6

Subunit structure

Associates with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor. Interacts with origin-binding protein. Interacts with the polymerase catalytic subunit. Ref.5 Ref.6

Subcellular location

Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae HEPA family.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentHost nucleus
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

viral genome replication

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.6. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

UL30P042934EBI-7185538,EBI-8615017

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 750750DNA helicase/primase complex-associated protein
PRO_0000115856

Regions

Compositional bias337 – 3437Poly-Ala

Natural variations

Natural variant2471T → M in strain: Nonneuroinvasive mutant HF10.
Natural variant3301A → T in strain: Nonneuroinvasive mutant HF10.
Natural variant3531A → V in strain: Nonneuroinvasive mutant HF10.
Natural variant4671N → D in strain: Nonneuroinvasive mutant HF10.
Natural variant4711A → V in strain: Nonneuroinvasive mutant HF10.
Natural variant543 – 5442AV → GL in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural variant6121C → G in strain: Nonneuroinvasive mutant HF10.

Sequences

Sequence LengthMass (Da)Tools
P10192 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: ECA9ABD0E85CB392

FASTA75079,926
        10         20         30         40         50         60 
MDTADIVWVE ESVSAITLYA VWLPPRAREY FHALVYFVCR NAAGEGRARF AEVSVTATEL 

        70         80         90        100        110        120 
RDFYGSADVS VQAVVAAARA ATTPAASPLE PLENPTLWRA LYACVLAALE RQTGPVALFA 

       130        140        150        160        170        180 
PLRIGSDPRT GLVVKVERAS WGPPAAPRAA LLVAEANIDI DPMALAARVA EHPDARLAWA 

       190        200        210        220        230        240 
RLAAIRDTPQ CASAASLTVN ITTGTALFAR EYQTLAFPPI KKEGAFGDLV EVCEVGLRPR 

       250        260        270        280        290        300 
GHPQRVTARV LLPRDYDYFV SAGEKFSAPA LVALFRQWHT TVHAAPGALA PVFAFLGPEF 

       310        320        330        340        350        360 
EVRGGPVPYF AVLGFPGWPT FTVPATAESA RDLVRGAAAA YAALLGAWPA VGARVVLPPR 

       370        380        390        400        410        420 
AWPGVASAAA GCLLPAVREA VARWHPATKI IQLLDPPAAV GPVWTARFCF PGLRAQLLAA 

       430        440        450        460        470        480 
LADLGGSGLA DPHGRTGLAR LDALVVAAPS EPWAGAVLER LVPDTCNACP ALRQLLGGVM 

       490        500        510        520        530        540 
AAVCLQIEET ASSVKFAVCG GDGGAFWGVF NVDPQDADAA SGVIEDARRA IETAVGAVLR 

       550        560        570        580        590        600 
ANAVRLRHPL CLALEGVYTH AVAWSQAGVW FWNSRDNTDH LGGFPLRGPA YTTAAGVVRD 

       610        620        630        640        650        660 
TLRRVLGLTT ACVPEEDALT ARGLMEDACD RLILDAFNKR LDAEYWSVRV SPFEASDPLP 

       670        680        690        700        710        720 
PTAFRGGALL DAEHYWRRVV RVCPGGGESV GVPVDLYPRP LVLPPVDCAH HLREILREIE 

       730        740        750 
LVFTGVLAGV WGEGGKFVYP FDDKMSFLFA 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Structures of herpes simplex virus type 1 genes required for replication of virus DNA."
McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D.
J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nonneuroinvasive mutant HF10.
[4]"Herpes simplex virus type 1 bacterial artificial chromosome."
Cunningham C., Davison A.J.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 17 syn+.
[5]"The herpes simplex virus type 1 origin-binding protein interacts specifically with the viral UL8 protein."
McLean G.W., Abbotts A.P., Parry M.E., Marsden H.S., Stow N.D.
J. Gen. Virol. 75:2699-2706(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL9, FUNCTION.
[6]"The catalytic subunit of the DNA polymerase of herpes simplex virus type 1 interacts specifically with the C terminus of the UL8 component of the viral helicase-primase complex."
Marsden H.S., McLean G.W., Barnard E.C., Francis G.J., MacEachran K., Murphy M., McVey G., Cross A., Abbotts A.P., Stow N.D.
J. Virol. 71:6390-6397(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL30, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32344.1.
M19120 Genomic DNA. Translation: AAA45823.1.
DQ889502 Genomic DNA. Translation: ABI63470.1.
FJ593289 Genomic DNA. Translation: ACM62230.1.
PIRWMBEX8. C29890.
RefSeqNP_044609.1. NC_001806.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid971456. 1 interaction.
DIPDIP-1097N.
IntActP10192. 2 interactions.
MINTMINT-6732582.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703432.

Family and domain databases

InterProIPR004996. DNA_helic/primase-assoc_herpes.
[Graphical view]
PfamPF03324. Herpes_HEPA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEPA_HHV11
AccessionPrimary (citable) accession number: P10192
Secondary accession number(s): B9VQD5, Q09IC5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families