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Protein

DNA helicase/primase complex-associated protein

Gene

UL8

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The primase-associated factor has no known catalytic activity in the complex and may serve to facilitate the formation of the replisome by directly interacting with the origin-binding protein and the polymerase.UniRule annotation2 Publications

GO - Biological processi

  • bidirectional double-stranded viral DNA replication Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase/primase complex-associated proteinUniRule annotation
Short name:
HEPAUniRule annotation
Alternative name(s):
Primase-associated factorUniRule annotation
Gene namesi
ORF Names:UL8
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

  • host cell nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001158561 – 750DNA helicase/primase complex-associated proteinAdd BLAST750

Proteomic databases

PRIDEiP10192.

Interactioni

Subunit structurei

Associates with the primase and the helicase to form the helicase-primase complex. Interacts with the origin-binding protein. Interacts with the polymerase catalytic subunit.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UL30P042934EBI-7185538,EBI-8615017

Protein-protein interaction databases

BioGridi971456. 1 interactor.
DIPiDIP-1097N.
IntActiP10192. 2 interactors.
MINTiMINT-6732582.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi337 – 343Poly-Ala7

Sequence similaritiesi

Belongs to the herpesviridae HEPA family.UniRule annotation

Family and domain databases

HAMAPiMF_04010. HSV_HEPA. 1 hit.
InterProiIPR004996. HSV_HEPA.
[Graphical view]
PfamiPF03324. Herpes_HEPA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTADIVWVE ESVSAITLYA VWLPPRAREY FHALVYFVCR NAAGEGRARF
60 70 80 90 100
AEVSVTATEL RDFYGSADVS VQAVVAAARA ATTPAASPLE PLENPTLWRA
110 120 130 140 150
LYACVLAALE RQTGPVALFA PLRIGSDPRT GLVVKVERAS WGPPAAPRAA
160 170 180 190 200
LLVAEANIDI DPMALAARVA EHPDARLAWA RLAAIRDTPQ CASAASLTVN
210 220 230 240 250
ITTGTALFAR EYQTLAFPPI KKEGAFGDLV EVCEVGLRPR GHPQRVTARV
260 270 280 290 300
LLPRDYDYFV SAGEKFSAPA LVALFRQWHT TVHAAPGALA PVFAFLGPEF
310 320 330 340 350
EVRGGPVPYF AVLGFPGWPT FTVPATAESA RDLVRGAAAA YAALLGAWPA
360 370 380 390 400
VGARVVLPPR AWPGVASAAA GCLLPAVREA VARWHPATKI IQLLDPPAAV
410 420 430 440 450
GPVWTARFCF PGLRAQLLAA LADLGGSGLA DPHGRTGLAR LDALVVAAPS
460 470 480 490 500
EPWAGAVLER LVPDTCNACP ALRQLLGGVM AAVCLQIEET ASSVKFAVCG
510 520 530 540 550
GDGGAFWGVF NVDPQDADAA SGVIEDARRA IETAVGAVLR ANAVRLRHPL
560 570 580 590 600
CLALEGVYTH AVAWSQAGVW FWNSRDNTDH LGGFPLRGPA YTTAAGVVRD
610 620 630 640 650
TLRRVLGLTT ACVPEEDALT ARGLMEDACD RLILDAFNKR LDAEYWSVRV
660 670 680 690 700
SPFEASDPLP PTAFRGGALL DAEHYWRRVV RVCPGGGESV GVPVDLYPRP
710 720 730 740 750
LVLPPVDCAH HLREILREIE LVFTGVLAGV WGEGGKFVYP FDDKMSFLFA
Length:750
Mass (Da):79,926
Last modified:July 1, 1989 - v1
Checksum:iECA9ABD0E85CB392
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti247T → M in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti330A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti353A → V in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti467N → D in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti471A → V in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti543 – 544AV → GL in strain: Nonneuroinvasive mutant HF10 and 17 syn+. 2
Natural varianti612C → G in strain: Nonneuroinvasive mutant HF10. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32344.1.
M19120 Genomic DNA. Translation: AAA45823.1.
DQ889502 Genomic DNA. Translation: ABI63470.1.
FJ593289 Genomic DNA. Translation: ACM62230.1.
PIRiC29890. WMBEX8.
RefSeqiYP_009137082.1. NC_001806.2.

Genome annotation databases

GeneIDi2703432.
KEGGivg:2703432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32344.1.
M19120 Genomic DNA. Translation: AAA45823.1.
DQ889502 Genomic DNA. Translation: ABI63470.1.
FJ593289 Genomic DNA. Translation: ACM62230.1.
PIRiC29890. WMBEX8.
RefSeqiYP_009137082.1. NC_001806.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi971456. 1 interactor.
DIPiDIP-1097N.
IntActiP10192. 2 interactors.
MINTiMINT-6732582.

Proteomic databases

PRIDEiP10192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703432.
KEGGivg:2703432.

Family and domain databases

HAMAPiMF_04010. HSV_HEPA. 1 hit.
InterProiIPR004996. HSV_HEPA.
[Graphical view]
PfamiPF03324. Herpes_HEPA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEPA_HHV11
AccessioniPrimary (citable) accession number: P10192
Secondary accession number(s): B9VQD5, Q09IC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 5, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.