P10192 (HEPA_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA helicase/primase complex-associated protein
Short name=HEPA

Alternative name(s):
Primase-associated factor

Gene names

ORF Names:

UL8

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The primase-associated factor has no known catalytic activity in the complex and may serve to facilitate the formation of the replisome by directly interacting with the origin-binding protein and the polymerase. Ref.5 Ref.6
Subunit structure	Associates with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor. Interacts with origin-binding protein. Interacts with the polymerase catalytic subunit. Ref.5 Ref.6
Subcellular location	Host nucleus By similarity.
Sequence similarities	Belongs to the herpesviridae HEPA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Host nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 750	750	DNA helicase/primase complex-associated protein		PRO_0000115856
Regions
	Compositional bias	337 – 343	7	Poly-Ala
Natural variations
	Natural variant	247	1	T → M in strain: Nonneuroinvasive mutant HF10.
	Natural variant	330	1	A → T in strain: Nonneuroinvasive mutant HF10.
	Natural variant	353	1	A → V in strain: Nonneuroinvasive mutant HF10.
	Natural variant	467	1	N → D in strain: Nonneuroinvasive mutant HF10.
	Natural variant	471	1	A → V in strain: Nonneuroinvasive mutant HF10.
	Natural variant	543 – 544	2	AV → GL in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
	Natural variant	612	1	C → G in strain: Nonneuroinvasive mutant HF10.

Sequences

	Sequence		Length	Mass (Da)
	P10192 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: ECA9ABD0E85CB392	FASTA	750	79,926
	10 20 30 40 50 60 MDTADIVWVE ESVSAITLYA VWLPPRAREY FHALVYFVCR NAAGEGRARF AEVSVTATEL 70 80 90 100 110 120 RDFYGSADVS VQAVVAAARA ATTPAASPLE PLENPTLWRA LYACVLAALE RQTGPVALFA 130 140 150 160 170 180 PLRIGSDPRT GLVVKVERAS WGPPAAPRAA LLVAEANIDI DPMALAARVA EHPDARLAWA 190 200 210 220 230 240 RLAAIRDTPQ CASAASLTVN ITTGTALFAR EYQTLAFPPI KKEGAFGDLV EVCEVGLRPR 250 260 270 280 290 300 GHPQRVTARV LLPRDYDYFV SAGEKFSAPA LVALFRQWHT TVHAAPGALA PVFAFLGPEF 310 320 330 340 350 360 EVRGGPVPYF AVLGFPGWPT FTVPATAESA RDLVRGAAAA YAALLGAWPA VGARVVLPPR 370 380 390 400 410 420 AWPGVASAAA GCLLPAVREA VARWHPATKI IQLLDPPAAV GPVWTARFCF PGLRAQLLAA 430 440 450 460 470 480 LADLGGSGLA DPHGRTGLAR LDALVVAAPS EPWAGAVLER LVPDTCNACP ALRQLLGGVM 490 500 510 520 530 540 AAVCLQIEET ASSVKFAVCG GDGGAFWGVF NVDPQDADAA SGVIEDARRA IETAVGAVLR 550 560 570 580 590 600 ANAVRLRHPL CLALEGVYTH AVAWSQAGVW FWNSRDNTDH LGGFPLRGPA YTTAAGVVRD 610 620 630 640 650 660 TLRRVLGLTT ACVPEEDALT ARGLMEDACD RLILDAFNKR LDAEYWSVRV SPFEASDPLP 670 680 690 700 710 720 PTAFRGGALL DAEHYWRRVV RVCPGGGESV GVPVDLYPRP LVLPPVDCAH HLREILREIE 730 740 750 LVFTGVLAGV WGEGGKFVYP FDDKMSFLFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Structures of herpes simplex virus type 1 genes required for replication of virus DNA." McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D. J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus." Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y. Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nonneuroinvasive mutant HF10.
[4]	"Herpes simplex virus type 1 bacterial artificial chromosome." Cunningham C., Davison A.J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 17 syn+.
[5]	"The herpes simplex virus type 1 origin-binding protein interacts specifically with the viral UL8 protein." McLean G.W., Abbotts A.P., Parry M.E., Marsden H.S., Stow N.D. J. Gen. Virol. 75:2699-2706(1994) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL9, FUNCTION.
[6]	"The catalytic subunit of the DNA polymerase of herpes simplex virus type 1 interacts specifically with the C terminus of the UL8 component of the viral helicase-primase complex." Marsden H.S., McLean G.W., Barnard E.C., Francis G.J., MacEachran K., Murphy M., McVey G., Cross A., Abbotts A.P., Stow N.D. J. Virol. 71:6390-6397(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL30, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32344.1. M19120 Genomic DNA. Translation: AAA45823.1. DQ889502 Genomic DNA. Translation: ABI63470.1. FJ593289 Genomic DNA. Translation: ACM62230.1.
PIR	WMBEX8. C29890.
RefSeq	NP_044609.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1097N.
MINT	MINT-6732582.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703432.
Phylogenomic databases
ProtClustDB	PHA3144.
Family and domain databases
InterPro	IPR004996. DNA_helic/primase-assoc_herpes. [Graphical view]
Pfam	PF03324. Herpes_HEPA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEPA_HHV11

Accession

Primary (citable) accession number: P10192
Secondary accession number(s): B9VQD5, Q09IC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program