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P10192

- HEPA_HHV11

UniProt

P10192 - HEPA_HHV11

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Protein

DNA helicase/primase complex-associated protein

Gene

UL8

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The primase-associated factor has no known catalytic activity in the complex and may serve to facilitate the formation of the replisome by directly interacting with the origin-binding protein and the polymerase.2 Publications

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase/primase complex-associated protein
Short name:
HEPA
Alternative name(s):
Primase-associated factor
Gene namesi
ORF Names:UL8
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750DNA helicase/primase complex-associated proteinPRO_0000115856Add
BLAST

Interactioni

Subunit structurei

Associates with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor. Interacts with origin-binding protein. Interacts with the polymerase catalytic subunit.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UL30P042934EBI-7185538,EBI-8615017

Protein-protein interaction databases

BioGridi971456. 1 interaction.
DIPiDIP-1097N.
IntActiP10192. 2 interactions.
MINTiMINT-6732582.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 3437Poly-Ala

Sequence similaritiesi

Belongs to the herpesviridae HEPA family.Curated

Family and domain databases

InterProiIPR004996. DNA_helic/primase-assoc_herpes.
[Graphical view]
PfamiPF03324. Herpes_HEPA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10192-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTADIVWVE ESVSAITLYA VWLPPRAREY FHALVYFVCR NAAGEGRARF
60 70 80 90 100
AEVSVTATEL RDFYGSADVS VQAVVAAARA ATTPAASPLE PLENPTLWRA
110 120 130 140 150
LYACVLAALE RQTGPVALFA PLRIGSDPRT GLVVKVERAS WGPPAAPRAA
160 170 180 190 200
LLVAEANIDI DPMALAARVA EHPDARLAWA RLAAIRDTPQ CASAASLTVN
210 220 230 240 250
ITTGTALFAR EYQTLAFPPI KKEGAFGDLV EVCEVGLRPR GHPQRVTARV
260 270 280 290 300
LLPRDYDYFV SAGEKFSAPA LVALFRQWHT TVHAAPGALA PVFAFLGPEF
310 320 330 340 350
EVRGGPVPYF AVLGFPGWPT FTVPATAESA RDLVRGAAAA YAALLGAWPA
360 370 380 390 400
VGARVVLPPR AWPGVASAAA GCLLPAVREA VARWHPATKI IQLLDPPAAV
410 420 430 440 450
GPVWTARFCF PGLRAQLLAA LADLGGSGLA DPHGRTGLAR LDALVVAAPS
460 470 480 490 500
EPWAGAVLER LVPDTCNACP ALRQLLGGVM AAVCLQIEET ASSVKFAVCG
510 520 530 540 550
GDGGAFWGVF NVDPQDADAA SGVIEDARRA IETAVGAVLR ANAVRLRHPL
560 570 580 590 600
CLALEGVYTH AVAWSQAGVW FWNSRDNTDH LGGFPLRGPA YTTAAGVVRD
610 620 630 640 650
TLRRVLGLTT ACVPEEDALT ARGLMEDACD RLILDAFNKR LDAEYWSVRV
660 670 680 690 700
SPFEASDPLP PTAFRGGALL DAEHYWRRVV RVCPGGGESV GVPVDLYPRP
710 720 730 740 750
LVLPPVDCAH HLREILREIE LVFTGVLAGV WGEGGKFVYP FDDKMSFLFA
Length:750
Mass (Da):79,926
Last modified:July 1, 1989 - v1
Checksum:iECA9ABD0E85CB392
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti247 – 2471T → M in strain: Nonneuroinvasive mutant HF10.
Natural varianti330 – 3301A → T in strain: Nonneuroinvasive mutant HF10.
Natural varianti353 – 3531A → V in strain: Nonneuroinvasive mutant HF10.
Natural varianti467 – 4671N → D in strain: Nonneuroinvasive mutant HF10.
Natural varianti471 – 4711A → V in strain: Nonneuroinvasive mutant HF10.
Natural varianti543 – 5442AV → GL in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural varianti612 – 6121C → G in strain: Nonneuroinvasive mutant HF10.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32344.1.
M19120 Genomic DNA. Translation: AAA45823.1.
DQ889502 Genomic DNA. Translation: ABI63470.1.
FJ593289 Genomic DNA. Translation: ACM62230.1.
PIRiC29890. WMBEX8.
RefSeqiNP_044609.1. NC_001806.1.

Genome annotation databases

GeneIDi2703432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32344.1 .
M19120 Genomic DNA. Translation: AAA45823.1 .
DQ889502 Genomic DNA. Translation: ABI63470.1 .
FJ593289 Genomic DNA. Translation: ACM62230.1 .
PIRi C29890. WMBEX8.
RefSeqi NP_044609.1. NC_001806.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 971456. 1 interaction.
DIPi DIP-1097N.
IntActi P10192. 2 interactions.
MINTi MINT-6732582.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703432.

Family and domain databases

InterProi IPR004996. DNA_helic/primase-assoc_herpes.
[Graphical view ]
Pfami PF03324. Herpes_HEPA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Structures of herpes simplex virus type 1 genes required for replication of virus DNA."
    McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D.
    J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
    Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
    Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nonneuroinvasive mutant HF10.
  4. "Herpes simplex virus type 1 bacterial artificial chromosome."
    Cunningham C., Davison A.J.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 17 syn+.
  5. "The herpes simplex virus type 1 origin-binding protein interacts specifically with the viral UL8 protein."
    McLean G.W., Abbotts A.P., Parry M.E., Marsden H.S., Stow N.D.
    J. Gen. Virol. 75:2699-2706(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL9, FUNCTION.
  6. "The catalytic subunit of the DNA polymerase of herpes simplex virus type 1 interacts specifically with the C terminus of the UL8 component of the viral helicase-primase complex."
    Marsden H.S., McLean G.W., Barnard E.C., Francis G.J., MacEachran K., Murphy M., McVey G., Cross A., Abbotts A.P., Stow N.D.
    J. Virol. 71:6390-6397(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL30, FUNCTION.

Entry informationi

Entry nameiHEPA_HHV11
AccessioniPrimary (citable) accession number: P10192
Secondary accession number(s): B9VQD5, Q09IC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3