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Protein

DNA replication helicase

Gene

UL5

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The UL5 subunit has helicase-like motifs and therefore may act as the helicase subunit.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 1048ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • bidirectional double-stranded viral DNA replication Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication helicase (EC:3.6.4.-)
Gene namesi
ORF Names:UL5
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

  • Host nucleus 1 Publication

GO - Cellular componenti

  • host cell nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882DNA replication helicasePRO_0000115845Add
BLAST

Interactioni

Subunit structurei

Associates with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor.

Protein-protein interaction databases

BioGridi971446. 2 interactions.
DIPiDIP-1094N.
IntActiP10189. 1 interaction.

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae helicase family.Curated

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003840. DNA_helicase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02689. Herpes_Helicase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.

Sequencei

Sequence statusi: Complete.

P10189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAGGERQL DGQKPGPPHL QQPGDRPAVP GRAEAFLNFT SMHGVQPILK
60 70 80 90 100
RIRELSQQQL DGAQVPHLQW FRDVAALESP AGLPLREFPF AVYLITGNAG
110 120 130 140 150
SGKSTCVQTI NEVLDCVVTG ATRIAAQNMY AKLSGAFLSR PINTIFHEFG
160 170 180 190 200
FRGNHVQAQL GQYPYTLTSN PASLEDLQRR DLTYYWEVIL DLTKRALAAS
210 220 230 240 250
GGEELRNEFR ALAALERTLG LAEGALTRLA PATHGALPAF TRSNVIVIDE
260 270 280 290 300
AGLLGRHLLT AVVYCWWMIN ALYHTPQYAA RLRPVLVCVG SPTQTASLES
310 320 330 340 350
TFEHQKLRCS VRQSENVLTY LICNRTLREY ARLSYSWAIF INNKRCVEHE
360 370 380 390 400
FGNLMKVLEY GLPITEEHMQ FVDRFVVPEN YITNPANLPG WTRLFSSHKE
410 420 430 440 450
VSAYMAKLHA YLKVTREGEF VVFTLPVLTF VSVKEFDEYR RLTHQPGLTI
460 470 480 490 500
EKWLTANASR ITNYSQSQDQ DAGHMRCEVH SKQQLVVARN DVTYVLNSQI
510 520 530 540 550
AVTARLRKLV FGFSGTFRAF EAVLRDDSFV KTQGETSVEF AYRFLSRLIF
560 570 580 590 600
SGLISFYNFL QRPGLDATQR TLAYARMGEL TAEILSLRPK SSGVPTQASV
610 620 630 640 650
MADAGAPGER AFDFKQLGPR DGGPDDFPDD DLDVIFAGLD EQQLDVFYCH
660 670 680 690 700
YTPGEPETTA AVHTQFALLK RAFLGRFRIL QELFGEAFEV APFSTYVDNV
710 720 730 740 750
IFRGCEMLTG SPRGGLMSVA LQTDNYTLMG YTYARVFAFA DELRRRHATA
760 770 780 790 800
NVAELLEEAP LPYVVLRDQH GFMSVVNTNI SEFVESIDST ELAMAINADY
810 820 830 840 850
GISSKLAMTI TRSQGLSLDK VAICFTPGNL RLNSAYVAMS RTTSSEFLRM
860 870 880
NLNPLRERHE RDDVISEHIL SALRDPNVVI VY
Length:882
Mass (Da):98,715
Last modified:September 22, 2009 - v3
Checksum:i38FF9C5DD831BFDB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti860 – 8601E → D in AAA45819 (PubMed:2826807).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141K → R in strain: Nonneuroinvasive mutant HF10.
Natural varianti67 – 671H → R in strain: Nonneuroinvasive mutant HF10.
Natural varianti205 – 2051L → S in strain: Nonneuroinvasive mutant HF10.
Natural varianti662 – 6621V → I in strain: Nonneuroinvasive mutant HF10.
Natural varianti690 – 6901V → G in strain: Nonneuroinvasive mutant HF10.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32341.1.
M19119 Genomic DNA. Translation: AAA45819.1.
DQ889502 Genomic DNA. Translation: ABI63467.1.
FJ593289 Genomic DNA. Translation: ACM62227.1.
RefSeqiYP_009137079.1. NC_001806.2.

Genome annotation databases

GeneIDi2703420.
KEGGivg:2703420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32341.1.
M19119 Genomic DNA. Translation: AAA45819.1.
DQ889502 Genomic DNA. Translation: ABI63467.1.
FJ593289 Genomic DNA. Translation: ACM62227.1.
RefSeqiYP_009137079.1. NC_001806.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi971446. 2 interactions.
DIPiDIP-1094N.
IntActiP10189. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703420.
KEGGivg:2703420.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003840. DNA_helicase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02689. Herpes_Helicase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Structures of herpes simplex virus type 1 genes required for replication of virus DNA."
    McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D.
    J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
    Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
    Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nonneuroinvasive mutant HF10.
  4. "Herpes simplex virus type 1 bacterial artificial chromosome."
    Cunningham C., Davison A.J.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 17 syn+.
  5. "Herpes simplex virus type 1 gene products required for DNA replication: identification and overexpression."
    Olivo P.D., Nelson N.J., Challberg M.D.
    J. Virol. 63:196-204(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: KOS.

Entry informationi

Entry nameiHELI_HHV11
AccessioniPrimary (citable) accession number: P10189
Secondary accession number(s): B9VQD2, Q09IC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: September 22, 2009
Last modified: March 16, 2016
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.