Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P10186 (UNG_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uracil-DNA glycosylase

Short name=UDG
EC=3.2.2.27
Alternative name(s):
UNG
Gene names
ORF Names:UL2
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or deamination of cytosine. Therefore may reduce deleterious uracil incorporation into the viral genome, particularly, in terminally differentiated neurons which lack DNA repair enzymes. Ref.5 Ref.6

Catalytic activity

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148

Sequence similarities

Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Molecular functionGlycosidase
Hydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbase-excision repair

Inferred from electronic annotation. Source: InterPro

   Molecular_functionuracil DNA N-glycosylase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Uracil-DNA glycosylase HAMAP-Rule MF_00148
PRO_0000176185

Regions

Compositional bias198 – 2014Poly-Pro HAMAP-Rule MF_00148

Sites

Active site1781Proton acceptor

Natural variations

Natural variant381T → I in strain: Nonneuroinvasive mutant HF10.
Natural variant491T → N in strain: Nonneuroinvasive mutant HF10.
Natural variant531S → L in strain: Nonneuroinvasive mutant HF10.
Natural variant67 – 759ALLAALEAG → GAPRRPRGC in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural variant1261M → L in strain: Nonneuroinvasive mutant HF10.
Natural variant2801T → A in strain: Nonneuroinvasive mutant HF10.

Secondary structure

............................................. 334
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10186 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 7CD3B74FB43F00CD

FASTA33436,328
        10         20         30         40         50         60 
MKRACSRSPS PRRRPSSPRR TPPRDGTPPQ KADADDPTPG ASNDASTETR PGSGGEPAAC 

        70         80         90        100        110        120 
RSSGPAALLA ALEAGPAGVT FSSSAPPDPP MDLTNGGVSP AATSAPLDWT TFRRVFLIDD 

       130        140        150        160        170        180 
AWRPLMEPEL ANPLTAHLLA EYNRRCQTEE VLPPREDVFS WTRYCTPDEV RVVIIGQDPY 

       190        200        210        220        230        240 
HHPGQAHGLA FSVRANVPPP PSLRNVLAAV KNCYPEARMS GHGCLEKWAR DGVLLLNTTL 

       250        260        270        280        290        300 
TVKRGAAASH SRIGWDRFVG GVIRRLAARR PGLVFMLWGT HAQNAIRPDP RVHCVLKFSH 

       310        320        330 
PSPLSKVPFG TCQHFLVANR YLETRSISPI DWSV 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nonneuroinvasive mutant HF10.
[3]"Herpes simplex virus type 1 bacterial artificial chromosome."
Cunningham C., Davison A.J.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 17 syn+.
[4]"The structural basis of specific base-excision repair by uracil-DNA glycosylase."
Savva R., McAuley-Hecht K., Brown T., Pearl L.
Nature 373:487-493(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 91-334.
[5]"Nucleotide mimicry in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex."
Savva R., Pearl L.H.
Nat. Struct. Biol. 2:752-757(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 91-334, FUNCTION.
[6]"A comparative study of uracil-DNA glycosylases from human and herpes simplex virus type 1."
Krusong K., Carpenter E.P., Bellamy S.R., Savva R., Baldwin G.S.
J. Biol. Chem. 281:4983-4992(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 91-334, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32338.1.
DQ889502 Genomic DNA. Translation: ABI63464.1.
FJ593289 Genomic DNA. Translation: ACM62224.1.
PIRDGBEX2. B28133.
RefSeqNP_044603.1. NC_001806.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LAUX-ray1.80E91-334[»]
1UDGX-ray1.75A91-334[»]
1UDHX-ray1.75A91-334[»]
1UDIX-ray2.70E91-334[»]
2C53X-ray1.80A91-334[»]
2C56X-ray2.10A91-334[»]
4L5NX-ray2.16A/B96-334[»]
ProteinModelPortalP10186.
SMRP10186. Positions 107-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1506602.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703370.

Enzyme and pathway databases

SABIO-RKP10186.

Family and domain databases

Gene3D3.40.470.10. 1 hit.
HAMAPMF_00148. UDG.
InterProIPR018085. Ura-DNA_Glyclase_AS.
IPR002043. Ura_DNA_glycsylse.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERPTHR11264. PTHR11264. 1 hit.
PfamPF03167. UDG. 1 hit.
[Graphical view]
SMARTSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMSSF52141. SSF52141. 1 hit.
TIGRFAMsTIGR00628. ung. 1 hit.
PROSITEPS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10186.

Entry information

Entry nameUNG_HHV11
AccessionPrimary (citable) accession number: P10186
Secondary accession number(s): B9VQC9, Q09ID1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references