P10186 (UNG_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Alternative name(s):
UNG

Gene names

ORF Names:

UL2

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or deamination of cytosine. Therefore may reduce deleterious uracil incorporation into the viral genome, particularly, in terminally differentiated neurons which lack DNA repair enzymes. Ref.5 Ref.6
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: InterPro
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 334

334

Uracil-DNA glycosylase

PRO_0000176185

Regions

Compositional bias

198 – 201

4

Poly-Pro

Sites

Active site

178

1

Proton acceptor

Natural variations

Natural variant

38

1

T → I in strain: Nonneuroinvasive mutant HF10.

Natural variant

49

1

T → N in strain: Nonneuroinvasive mutant HF10.

Natural variant

53

1

S → L in strain: Nonneuroinvasive mutant HF10.

Natural variant

67 – 75

9

ALLAALEAG → GAPRRPRGC in strain: Nonneuroinvasive mutant HF10 and 17 syn+.

Natural variant

126

1

M → L in strain: Nonneuroinvasive mutant HF10.

Natural variant

280

1

T → A in strain: Nonneuroinvasive mutant HF10.

Secondary structure

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334

Helix Strand Turn

Details...

Helix

108 – 116

9

Helix

120 – 122

3

Helix

123 – 130

8

Helix

133 – 148

16

Beta strand

151 – 153

3

Turn

155 – 160

6

Helix

161 – 164

4

Helix

167 – 169

3

Beta strand

172 – 176

5

Turn

183 – 185

3

Beta strand

187 – 191

5

Helix

201 – 213

13

Helix

226 – 230

5

Beta strand

233 – 239

7

Turn

247 – 252

6

Helix

255 – 269

15

Beta strand

270 – 272

3

Beta strand

274 – 279

6

Helix

280 – 285

6

Turn

290 – 292

3

Beta strand

293 – 298

6

Beta strand

305 – 307

3

Helix

309 – 311

3

Helix

314 – 323

10

Turn

324 – 326

3

Sequences

	Sequence		Length	Mass (Da)
	P10186 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 7CD3B74FB43F00CD	FASTA	334	36,328
	10 20 30 40 50 60 MKRACSRSPS PRRRPSSPRR TPPRDGTPPQ KADADDPTPG ASNDASTETR PGSGGEPAAC 70 80 90 100 110 120 RSSGPAALLA ALEAGPAGVT FSSSAPPDPP MDLTNGGVSP AATSAPLDWT TFRRVFLIDD 130 140 150 160 170 180 AWRPLMEPEL ANPLTAHLLA EYNRRCQTEE VLPPREDVFS WTRYCTPDEV RVVIIGQDPY 190 200 210 220 230 240 HHPGQAHGLA FSVRANVPPP PSLRNVLAAV KNCYPEARMS GHGCLEKWAR DGVLLLNTTL 250 260 270 280 290 300 TVKRGAAASH SRIGWDRFVG GVIRRLAARR PGLVFMLWGT HAQNAIRPDP RVHCVLKFSH 310 320 330 PSPLSKVPFG TCQHFLVANR YLETRSISPI DWSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus." Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y. Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nonneuroinvasive mutant HF10.
[3]	"Herpes simplex virus type 1 bacterial artificial chromosome." Cunningham C., Davison A.J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 17 syn+.
[4]	"The structural basis of specific base-excision repair by uracil-DNA glycosylase." Savva R., McAuley-Hecht K., Brown T., Pearl L. Nature 373:487-493(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 91-334.
[5]	"Nucleotide mimicry in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex." Savva R., Pearl L.H. Nat. Struct. Biol. 2:752-757(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 91-334, FUNCTION.
[6]	"A comparative study of uracil-DNA glycosylases from human and herpes simplex virus type 1." Krusong K., Carpenter E.P., Bellamy S.R., Savva R., Baldwin G.S. J. Biol. Chem. 281:4983-4992(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 91-334, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X14112 Genomic DNA. Translation: CAA32338.1.
DQ889502 Genomic DNA. Translation: ABI63464.1.
FJ593289 Genomic DNA. Translation: ACM62224.1.

PIR

DGBEX2. B28133.

RefSeq

NP_044603.1. NC_001806.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LAU	X-ray	1.80	E	91-334	[»]
1UDG	X-ray	1.75	A	91-334	[»]
1UDH	X-ray	1.75	A	91-334	[»]
1UDI	X-ray	2.70	E	91-334	[»]
2C53	X-ray	1.80	A	91-334	[»]
2C56	X-ray	2.10	A	91-334	[»]

ProteinModelPortal

P10186.

SMR

P10186. Positions 107-334.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2703370.

Phylogenomic databases

ProtClustDB

PHA3201.

Family and domain databases

Gene3D

3.40.470.10. 1 hit.

InterPro

IPR018085. Ura-DNA_Glyclase_AS.
IPR002043. Ura_DNA_glycsylse.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]

PANTHER

PTHR11264. PTHR11264. 1 hit.

Pfam

PF03167. UDG. 1 hit.
[Graphical view]

SMART

SM00986. UDG. 1 hit.
[Graphical view]

SUPFAM

SSF52141. UDNA_glycsylseSF. 1 hit.

TIGRFAMs

TIGR00628. ung. 1 hit.

PROSITE

PS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P10186.

Entry information

Entry name

UNG_HHV11

Accession

Primary (citable) accession number: P10186
Secondary accession number(s): B9VQC9, Q09ID1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program