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P10186

- UNG_HHV11

UniProt

P10186 - UNG_HHV11

Protein

Uracil-DNA glycosylase

Gene

UL2

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or deamination of cytosine. Therefore may reduce deleterious uracil incorporation into the viral genome, particularly, in terminally differentiated neurons which lack DNA repair enzymes.2 Publications

    Catalytic activityi

    Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei178 – 1781Proton acceptor

    GO - Molecular functioni

    1. uracil DNA N-glycosylase activity Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Enzyme and pathway databases

    SABIO-RKP10186.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uracil-DNA glycosylase (EC:3.2.2.27)
    Short name:
    UDG
    Alternative name(s):
    UNG
    Gene namesi
    ORF Names:UL2
    OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10299 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009294: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 334334Uracil-DNA glycosylasePRO_0000176185Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1506602.

    Structurei

    Secondary structure

    1
    334
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi108 – 1169
    Helixi120 – 1223
    Helixi123 – 1308
    Helixi133 – 14816
    Beta strandi151 – 1533
    Turni155 – 1606
    Helixi161 – 1644
    Helixi167 – 1693
    Beta strandi172 – 1765
    Turni183 – 1853
    Beta strandi187 – 1915
    Helixi201 – 21313
    Helixi226 – 2305
    Beta strandi233 – 2397
    Turni247 – 2526
    Helixi255 – 26915
    Beta strandi270 – 2723
    Beta strandi274 – 2796
    Helixi280 – 2856
    Turni290 – 2923
    Beta strandi293 – 2986
    Beta strandi305 – 3073
    Helixi309 – 3113
    Helixi314 – 32310
    Turni324 – 3263

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LAUX-ray1.80E91-334[»]
    1UDGX-ray1.75A91-334[»]
    1UDHX-ray1.75A91-334[»]
    1UDIX-ray2.70E91-334[»]
    2C53X-ray1.80A91-334[»]
    2C56X-ray2.10A91-334[»]
    4L5NX-ray2.16A/B96-334[»]
    ProteinModelPortaliP10186.
    SMRiP10186. Positions 107-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10186.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi198 – 2014Poly-Pro

    Sequence similaritiesi

    Belongs to the uracil-DNA glycosylase family.Curated

    Family and domain databases

    Gene3Di3.40.470.10. 1 hit.
    HAMAPiMF_00148. UDG.
    InterProiIPR018085. Ura-DNA_Glyclase_AS.
    IPR002043. Ura_DNA_glycsylse.
    IPR005122. Uracil-DNA_glycosylase-like.
    [Graphical view]
    PANTHERiPTHR11264. PTHR11264. 1 hit.
    PfamiPF03167. UDG. 1 hit.
    [Graphical view]
    SMARTiSM00986. UDG. 1 hit.
    [Graphical view]
    SUPFAMiSSF52141. SSF52141. 1 hit.
    TIGRFAMsiTIGR00628. ung. 1 hit.
    PROSITEiPS00130. U_DNA_GLYCOSYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10186-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRACSRSPS PRRRPSSPRR TPPRDGTPPQ KADADDPTPG ASNDASTETR    50
    PGSGGEPAAC RSSGPAALLA ALEAGPAGVT FSSSAPPDPP MDLTNGGVSP 100
    AATSAPLDWT TFRRVFLIDD AWRPLMEPEL ANPLTAHLLA EYNRRCQTEE 150
    VLPPREDVFS WTRYCTPDEV RVVIIGQDPY HHPGQAHGLA FSVRANVPPP 200
    PSLRNVLAAV KNCYPEARMS GHGCLEKWAR DGVLLLNTTL TVKRGAAASH 250
    SRIGWDRFVG GVIRRLAARR PGLVFMLWGT HAQNAIRPDP RVHCVLKFSH 300
    PSPLSKVPFG TCQHFLVANR YLETRSISPI DWSV 334
    Length:334
    Mass (Da):36,328
    Last modified:July 1, 1989 - v1
    Checksum:i7CD3B74FB43F00CD
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti38 – 381T → I in strain: Nonneuroinvasive mutant HF10.
    Natural varianti49 – 491T → N in strain: Nonneuroinvasive mutant HF10.
    Natural varianti53 – 531S → L in strain: Nonneuroinvasive mutant HF10.
    Natural varianti67 – 759ALLAALEAG → GAPRRPRGC in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
    Natural varianti126 – 1261M → L in strain: Nonneuroinvasive mutant HF10.
    Natural varianti280 – 2801T → A in strain: Nonneuroinvasive mutant HF10.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32338.1.
    DQ889502 Genomic DNA. Translation: ABI63464.1.
    FJ593289 Genomic DNA. Translation: ACM62224.1.
    PIRiB28133. DGBEX2.
    RefSeqiNP_044603.1. NC_001806.1.

    Genome annotation databases

    GeneIDi2703370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32338.1 .
    DQ889502 Genomic DNA. Translation: ABI63464.1 .
    FJ593289 Genomic DNA. Translation: ACM62224.1 .
    PIRi B28133. DGBEX2.
    RefSeqi NP_044603.1. NC_001806.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LAU X-ray 1.80 E 91-334 [» ]
    1UDG X-ray 1.75 A 91-334 [» ]
    1UDH X-ray 1.75 A 91-334 [» ]
    1UDI X-ray 2.70 E 91-334 [» ]
    2C53 X-ray 1.80 A 91-334 [» ]
    2C56 X-ray 2.10 A 91-334 [» ]
    4L5N X-ray 2.16 A/B 96-334 [» ]
    ProteinModelPortali P10186.
    SMRi P10186. Positions 107-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1506602.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703370.

    Enzyme and pathway databases

    SABIO-RK P10186.

    Miscellaneous databases

    EvolutionaryTracei P10186.

    Family and domain databases

    Gene3Di 3.40.470.10. 1 hit.
    HAMAPi MF_00148. UDG.
    InterProi IPR018085. Ura-DNA_Glyclase_AS.
    IPR002043. Ura_DNA_glycsylse.
    IPR005122. Uracil-DNA_glycosylase-like.
    [Graphical view ]
    PANTHERi PTHR11264. PTHR11264. 1 hit.
    Pfami PF03167. UDG. 1 hit.
    [Graphical view ]
    SMARTi SM00986. UDG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52141. SSF52141. 1 hit.
    TIGRFAMsi TIGR00628. ung. 1 hit.
    PROSITEi PS00130. U_DNA_GLYCOSYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
      J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
      Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
      Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nonneuroinvasive mutant HF10.
    3. "Herpes simplex virus type 1 bacterial artificial chromosome."
      Cunningham C., Davison A.J.
      Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 17 syn+.
    4. "The structural basis of specific base-excision repair by uracil-DNA glycosylase."
      Savva R., McAuley-Hecht K., Brown T., Pearl L.
      Nature 373:487-493(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 91-334.
    5. "Nucleotide mimicry in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex."
      Savva R., Pearl L.H.
      Nat. Struct. Biol. 2:752-757(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 91-334, FUNCTION.
    6. "A comparative study of uracil-DNA glycosylases from human and herpes simplex virus type 1."
      Krusong K., Carpenter E.P., Bellamy S.R., Savva R., Baldwin G.S.
      J. Biol. Chem. 281:4983-4992(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 91-334, FUNCTION.

    Entry informationi

    Entry nameiUNG_HHV11
    AccessioniPrimary (citable) accession number: P10186
    Secondary accession number(s): B9VQC9, Q09ID1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3