P10185 (GL_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein L
Short name=gL

Gene names

Name:	gL
ORF Names:	UL1

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding of gD to one of its receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Ref.7
Subunit structure	Interacts with glycoprotein H (gH); this interaction is necessary for the correct processing and cell surface expression of gH. The heterodimer gH/gL seems to interact with gB trimers during fusion. Associates with the gB-gH/gL-gD complex By similarity. Ref.5 Ref.8
Subcellular location	Virion membrane; Peripheral membrane protein; Extracellular side. Host cell membrane; Peripheral membrane protein; Extracellular side By similarity. Note: gL is not anchored to the viral envelope, but associates with the extravirion surface through its binding to gH. During virion morphogenesis, this protein probably accumulates in the trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN) By similarity. A fraction of gL has also been found associated to the cell surface in the absence of gH, suggesting incomplete cleavage of the signal peptide or presence of a cell surface receptor for secreted gL. Ref.6 Ref.8
Post-translational modification	N-glycosylated, O-glycosylated, and sialylated By similarity.
Sequence similarities	Belongs to the herpesviridae glycoprotein L family.

Ontologies

Keywords
Biological process	Fusion of virus membrane with host cell membrane Fusion of virus membrane with host membrane Viral penetration into host cytoplasm Virus entry into host cell
Cellular component	Host cell membrane Host membrane Membrane Viral envelope protein Virion
Domain	Signal
Ligand	Sialic acid
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	viral entry into host cell via membrane fusion with the plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 224	205	Envelope glycoprotein L		PRO_0000038264
Regions
	Region	20 – 161	142	Interaction with gL
	Compositional bias	215 – 218	4	Poly-Arg
Amino acid modifications
	Glycosylation	170	1	N-linked (GlcNAc...); by host Probable
Natural variations
	Natural variant	90	1	K → R in strain: Nonneuroinvasive mutant HF10.
	Natural variant	100	1	V → G in strain: Nonneuroinvasive mutant HF10.
	Natural variant	109	1	Y → H in strain: Nonneuroinvasive mutant HF10.
	Natural variant	115	1	N → D in strain: Nonneuroinvasive mutant HF10.
	Natural variant	168	1	P → L in strain: Nonneuroinvasive mutant HF10.
	Natural variant	171	1	G → R in strain: Nonneuroinvasive mutant HF10.
	Natural variant	181	1	P → S in strain: Nonneuroinvasive mutant HF10.
	Natural variant	196	1	P → S in strain: Nonneuroinvasive mutant HF10.
	Natural variant	224	1	L → I in strain: Nonneuroinvasive mutant HF10.

Sequences

	Sequence		Length	Mass (Da)
	P10185 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: CED51D2FC1D7777F	FASTA	224	24,934
	10 20 30 40 50 60 MGILGWVGLI AVGVLCVRGG LPSTEYVIRS RVAREVGDIL KVPCVPLPSD DLDWRYETPS 70 80 90 100 110 120 AINYALIDGI FLRYHCPGLD TVLWDRHAQK AYWVNPFLFV AGFLEDLSYP AFPANTQETE 130 140 150 160 170 180 TRLALYKEIR QALDSRKQAA SHTPVKAGCV NFDYSRTRRC VGRQDLGPTN GTSGRTPVLP 190 200 210 220 PDDEAGLQPK PLTTPPPIIA TSDPTPRRDA ATKSRRRRPH SRRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The DNA sequences of the long repeat region and adjoining parts of the long unique region in the genome of herpes simplex virus type 1." Perry L.J., McGeoch D.J. J. Gen. Virol. 69:2831-2846(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus." Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y. Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nonneuroinvasive mutant HF10.
[4]	"Herpes simplex virus type 1 bacterial artificial chromosome." Cunningham C., Davison A.J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 17 syn+.
[5]	"Structure-function analysis of herpes simplex virus type 1 gD and gH-gL: clues from gDgH chimeras." Cairns T.M., Milne R.S., Ponce-de-Leon M., Tobin D.K., Cohen G.H., Eisenberg R.J. J. Virol. 77:6731-6742(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GH.
[6]	"Comprehensive characterization of extracellular herpes simplex virus type 1 virions." Loret S., Guay G., Lippe R. J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: F.
[7]	"Herpes simplex virus gD forms distinct complexes with fusion executors gB and gH/gL in part through the C-terminal profusion domain." Gianni T., Amasio M., Campadelli-Fiume G. J. Biol. Chem. 284:17370-17382(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: F.
[8]	"Insertional mutations in herpes simplex virus 1 gL identify functional domains for association with gH and for membrane fusion." Fan Q., Lin E., Spear P.G. J. Virol. 83:11607-11615(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GH, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32337.1. D00373 Genomic DNA. Translation: BAA00272.1. DQ889502 Genomic DNA. Translation: ABI63463.1. FJ593289 Genomic DNA. Translation: ACM62223.1.
PIR	WMBEX1. A28133.
RefSeq	NP_044602.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703393.
Phylogenomic databases
ProtClustDB	PHA3301.
Family and domain databases
InterPro	IPR022200. Herpes_gL_C. IPR007923. Herpes_gL_N. [Graphical view]
Pfam	PF12524. GlyL_C. 1 hit. PF05259. Herpes_UL1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GL_HHV11

Accession

Primary (citable) accession number: P10185
Secondary accession number(s): Q09ID2, Q76WT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program