P10180 (CUT_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein cut

Gene names

Name:	ct
ORF Names:	CG11387

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	2175 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of cell fate decisions in multiple lineages. Specifically, functions as a determination factor that specifies sensory organ identity in precursor cells. Probably also involved in cell type specification of Malpighian tubules. In absence of cut gene external sensory organs are transformed into chordotonal organs.
Subcellular location	Nucleus Probable.
Tissue specificity	Detected in many cells in the central nervous system, all external sensory organs, some peripheral neurons, and in the non-neural cells of the spiracles and the Malpighian tubules.
Developmental stage	Cell-specific pattern of expression. Broadly expressed during embryonic development.
Miscellaneous	Asn-1791 may participate in regulating DNA-binding activity by promoting homo- and heterodimerization.
Sequence similarities	Belongs to the CUT homeobox family. Contains 3 CUT DNA-binding domains. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil Homeobox Repeat
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Malpighian tubule bud morphogenesis Inferred from genetic interaction PubMed 17190812. Source: FlyBase antennal joint development Inferred from mutant phenotype PubMed 18820445. Source: FlyBase central nervous system development Traceable author statement PubMed 11810238. Source: FlyBase dendrite guidance Inferred from mutant phenotype PubMed 17276922. Source: FlyBase female gonad development Traceable author statement PubMed 11810238. Source: FlyBase formation of a compartment boundary Inferred from mutant phenotype PubMed 21795284. Source: FlyBase imaginal disc-derived wing margin morphogenesis Inferred from mutant phenotype PubMed 11810238. Source: FlyBase mitotic cell cycle Inferred from mutant phenotype PubMed 16141223. Source: FlyBase negative regulation of Notch signaling pathway Inferred from mutant phenotype PubMed 16141223. Source: FlyBase negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 9774673. Source: FlyBase ovarian follicle cell stalk formation Traceable author statement PubMed 10822261. Source: FlyBase peripheral nervous system development Traceable author statement PubMed 11810238PubMed 15018941. Source: FlyBase sensory perception of sound Inferred from mutant phenotype PubMed 18820445. Source: FlyBase spiracle morphogenesis, open tracheal system Inferred from mutant phenotype PubMed 10491268. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay PubMed 1977661PubMed 9774673. Source: FlyBase
Molecular_function	sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription regulatory region sequence-specific DNA binding Inferred from direct assay PubMed 9774673. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2175	2175	Homeobox protein cut		PRO_0000202390
Regions
	DNA binding	877 – 964	88	CUT 1
	DNA binding	1330 – 1417	88	CUT 2
	DNA binding	1608 – 1695	88	CUT 3
	DNA binding	1745 – 1804	60	Homeobox
	Coiled coil	265 – 343	79	Potential
	Coiled coil	433 – 499	67	Potential
	Coiled coil	1056 – 1161	106	Potential
	Coiled coil	1463 – 1522	60	Potential
	Compositional bias	194 – 210	17	Ala/Gln-rich
	Compositional bias	235 – 243	9	Ala-rich
	Compositional bias	271 – 293	23	Asp/Glu-rich (acidic)
	Compositional bias	384 – 428	45	Asn-rich
	Compositional bias	547 – 554	8	Asp/Glu-rich (acidic)
	Compositional bias	574 – 584	11	Asp/Glu-rich (acidic)
	Compositional bias	616 – 630	15	Ala-rich
	Compositional bias	665 – 699	35	Gln/His-rich (OPA repeat)
	Compositional bias	2004 – 2014	11	Ala-rich
	Compositional bias	2071 – 2077	7	Asp/Glu-rich (acidic)
	Compositional bias	2124 – 2136	13	Ala/Pro-rich
Amino acid modifications
	Modified residue	1940	1	Phosphoserine Ref.4
	Modified residue	1944	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P10180 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 08BF80C4861BD0AB	FASTA	2,175	233,629
	10 20 30 40 50 60 MQPTLPQAAG TADMDLTAVQ SINDWFFKKE QIYLLAQFWQ QRATLAEKEV NTLKEQLSTG 70 80 90 100 110 120 NPDSNLNSEN SDTAAAAATA AAVAAVVAGA TATNDIEDEQ QQQLQQTASG GILESDSDKL 130 140 150 160 170 180 LNSSIVAAAI TLQQQNGSNL LANTNTPSPS PPLLSAEQQQ QLQSSLQQSG GVGGACLNPK 190 200 210 220 230 240 LFFNHAQQMM MMEAAAAAAA AALQQQQQQQ SPLHSPANEV AIPTEQPAAT VATGAAAAAA 250 260 270 280 290 300 AAATPIATGN VKSGSTTSNA NHTNSNNSHQ DEEELDDEEE DEEEDEDEDD EEENASMQSN 310 320 330 340 350 360 ADDMELDAQQ ETRTEPSATT QQQHQQQDTE DLEENKDAGE ASLNVSNNHN TTDSNNSCSR 370 380 390 400 410 420 KNNNGGNESE QHVASSAEDD DCANNNTNTS NNNNTSNTAT SNTNNNNNNN SSSGNSEKRK 430 440 450 460 470 480 KKNNNNNNGQ PAVLLAAKDK EIKALLDELQ RLRAQEQTHL VQIQRLEEHL EVKRQHIIRL 490 500 510 520 530 540 EARLDKQQIN EALAEATALS AAASTNNNNN SQSSDNNKKL NTAAERPMDA SSNADLPEST 550 560 570 580 590 600 KAPVPAEDDE EDEDQAMLVD SEEAEDKPED SHHDDDEDED EDREAVNATT TDSNELKIKK 610 620 630 640 650 660 EQHSPLDLNV LSPNSAIAAA AAAAAAAACA NDPNKFQALL IERTKALAAE ALKNGASDAL 670 680 690 700 710 720 SEDAHHQQQQ HHQQQHQHQQ QHHQQQHLHQ QHHHHLQQQP NSGSNSNPAS NDHHHGHHLH 730 740 750 760 770 780 GHGLLHPSSA HHLHHQTTES NSNSSTPTAA GNNNGSNNSS SNTNANSTAQ LAASLASTLN 790 800 810 820 830 840 GTKSLMQEDS NGLAAVAMAA HAQHAAALGP GFLPGLPAFQ FAAAQVAAGG DGRGHYRFAD 850 860 870 880 890 900 SELQLPPGAS MAGRLGESLI PKGDPMEAKL QEMLRYNMDK YANQALDTLH ISRRVRELLS 910 920 930 940 950 960 VHNIGQRLFA KYILGLSQGT VSELLSKPKP WDKLTEKGRD SYRKMHAWAC DDNAVMLLKS 970 980 990 1000 1010 1020 LIPKKDSGLP QYAGRGAGGA GGDDSMSEDR IAHILSEASS LMKQSSVAQH REQERRSHGG 1030 1040 1050 1060 1070 1080 EDSHSNEDSK SPPQSCTSPF FKVENQLKQH QHLNPEQAAA QQREREREQR EREQQQRLRH 1090 1100 1110 1120 1130 1140 DDQDKMARLY QELIARTPRE TAFPSFLFSP SLFGGAAGMP GAASNAFPAM ADENMRHVFE 1150 1160 1170 1180 1190 1200 REIAKLQQHQ QQQQAAQAQA QFPNFSSLMA LQQQVLNGAQ DLSLAAAAAK DIKLNGQRSS 1210 1220 1230 1240 1250 1260 LEHSAGSSSC SKDGERDDAY PSSLHGRKSE GGGTPAPPAP PSGPGTGAGA PPTAAPPTGG 1270 1280 1290 1300 1310 1320 ASSNSAAPSP LSNSILPPAL SSQGEEFAAT ASPLQRMASI TNSLITQPPV TPHHSTPQRP 1330 1340 1350 1360 1370 1380 TKAVLPPITQ QQFDMFNNLN TEDIVRRVKE ALSQYSISQR LFGESVLGLS QGSVSDLLAR 1390 1400 1410 1420 1430 1440 PKPWHMLTQK GREPFIRMKM FLEDENAVHK LVASQYKIAP EKLMRTGSYS GSPQMPQGLA 1450 1460 1470 1480 1490 1500 SKMQAASLPM QKMMSELKLQ EPAQAQHLMQ QMQAAAMSAA MQQQQVAQAQ QQAQQAQQAQ 1510 1520 1530 1540 1550 1560 QHLQQQAQQH LQQQQHLAQQ QHPHQQHHQA AAAAAALHHQ SMLLTSPGLP PQHAISLPPS 1570 1580 1590 1600 1610 1620 AGGAQPGGPG GNQGSSNPSN SEKKPMLMPV HGTNAMRSLH QHMSPTVYEM AALTQDLDTH 1630 1640 1650 1660 1670 1680 DITTKIKEAL LANNIGQKIF GEAVLGLSQG SVSELLSKPK PWHMLSIKGR EPFIRMQLWL 1690 1700 1710 1720 1730 1740 SDANNVERLQ LLKNERREAS KRRRSTGPNQ QDNSSDTSSN DTNDFYTSSP GPGSVGSGVG 1750 1760 1770 1780 1790 1800 GAPPSKKQRV LFSEEQKEAL RLAFALDPYP NVGTIEFLAN ELGLATRTIT NWFHNHRMRL 1810 1820 1830 1840 1850 1860 KQQVPHGPAG QDNPIPSRES TSATPFDPVQ FRILLQQRLL ELHKERMGMS GAPIPYPPYF 1870 1880 1890 1900 1910 1920 AAAAILGRSL AGIPGAAAAA GAAAAAAAVG ASGGDELQAL NQAFKEQMSG LDLSMPTLKR 1930 1940 1950 1960 1970 1980 ERSDDYQDDL ELEGGGHNLS DNESLEGQEP EDKTTDYEKV LHKSALAAAA AYMSNAVRSS 1990 2000 2010 2020 2030 2040 RRKPAAPQWV NPAGAVTNPS AVVAAVAAAA AAAADNERII NGVCVMQASE YGRDDTDSNK 2050 2060 2070 2080 2090 2100 PTDGGNDSDH EHAQLEIDQR FMEPEVHIKQ EEDDDEEQSG SVNLDNEDNA TSEQKLKVIN 2110 2120 2130 2140 2150 2160 EEKLRMVRVR RLSSTGGGSS EEMPAPLAPP PPPPAASSSI VSGESTTSSS SSSNTSSSTP 2170 AVTTAAATAA AGWNY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and expression of a product from cut, a locus involved in specifying sensory organ identity in Drosophila." Blochlinger K., Bodmer R., Jack J., Jan L.Y., Jan Y.N. Nature 333:629-635(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1940 AND SER-1944, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07985 mRNA. Translation: CAA30794.1. AE014298 Genomic DNA. Translation: AAF46264.2.
PIR	S03170.
RefSeq	NP_524764.1. NM_080025.2.
UniGene	Dm.1827.
3D structure databases
ProteinModelPortal	P10180.
SMR	P10180. Positions 886-968, 1330-1448, 1613-1804.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19788N.
IntAct	P10180. 1 interaction.
MINT	MINT-941430.
Proteomic databases
PaxDb	P10180.
PRIDE	P10180.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0071068; FBpp0071026; FBgn0004198.
GeneID	44540.
KEGG	dme:Dmel_CG11387.
UCSC	CG11387-RA. d. melanogaster.
Organism-specific databases
CTD	44540.
FlyBase	FBgn0004198. ct.
Phylogenomic databases
eggNOG	NOG306581.
GeneTree	ENSGT00530000063019.
InParanoid	P10180.
KO	K09313.
OrthoDB	EOG4HT77Z.
Gene expression databases
Bgee	P10180.
GermOnline	CG11387. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.260.40. 3 hits.
InterPro	IPR003350. Hmoeo_CUT. IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR010982. Lambda_DNA-bd_dom. [Graphical view]
Pfam	PF02376. CUT. 3 hits. PF00046. Homeobox. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 3 hits.
PROSITE	PS51042. CUT. 3 hits. PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PCYT1A. drosophila.
GenomeRNAi	44540.
NextBio	837385.

Entry information

Entry name

CUT_DROME

Accession

Primary (citable) accession number: P10180
Secondary accession number(s): Q9W3Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 141 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project