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Protein

Cytochrome c oxidase subunit 8B, mitochondrial

Gene

COX8B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 8B, mitochondrial
Alternative name(s):
Cytochrome c oxidase polypeptide VIII-heart
Cytochrome c oxidase subunit 8-1
Cytochrome c oxidase subunit 8H
IX
VIIIb
Gene namesi
Name:COX8B
Synonyms:COX8H
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 11

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 3511Mitochondrial matrixAdd
BLAST
Transmembranei36 – 5924HelicalAdd
BLAST
Topological domaini60 – 7011Mitochondrial intermembraneAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-SubCell
  3. respiratory chain complex IV Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion2 PublicationsAdd
BLAST
Chaini25 – 7046Cytochrome c oxidase subunit 8B, mitochondrialPRO_0000006176Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-60941N.
STRINGi9913.ENSBTAP00000002651.

Structurei

Secondary structure

1
70
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 5924Combined sources
Helixi61 – 655Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OCCX-ray2.80M/Z25-70[»]
1OCOX-ray2.80M/Z25-70[»]
1OCRX-ray2.35M/Z25-70[»]
1OCZX-ray2.90M/Z25-70[»]
1V54X-ray1.80M/Z25-70[»]
1V55X-ray1.90M/Z25-70[»]
2DYRX-ray1.80M/Z25-70[»]
2DYSX-ray2.20M/Z25-70[»]
2EIJX-ray1.90M/Z25-70[»]
2EIKX-ray2.10M/Z25-70[»]
2EILX-ray2.10M/Z25-70[»]
2EIMX-ray2.60M/Z25-70[»]
2EINX-ray2.70M/Z25-70[»]
2OCCX-ray2.30M/Z25-70[»]
2Y69X-ray1.95M/Z1-70[»]
2YBBelectron microscopy19.00X25-70[»]
2ZXWX-ray2.50M/Z25-70[»]
3ABKX-ray2.00M/Z25-70[»]
3ABLX-ray2.10M/Z25-70[»]
3ABMX-ray1.95M/Z25-70[»]
3AG1X-ray2.20M/Z25-70[»]
3AG2X-ray1.80M/Z25-70[»]
3AG3X-ray1.80M/Z25-70[»]
3AG4X-ray2.05M/Z25-70[»]
3ASNX-ray3.00M/Z25-70[»]
3ASOX-ray2.30M/Z25-70[»]
3WG7X-ray1.90M/Z25-70[»]
ProteinModelPortaliP10175.
SMRiP10175. Positions 25-67.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10175.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c oxidase VIII family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG83531.
GeneTreeiENSGT00530000065013.
HOGENOMiHOG000230478.
InParanoidiP10175.
KOiK02273.
OrthoDBiEOG769ZNX.
TreeFamiTF105070.

Family and domain databases

Gene3Di4.10.81.10. 1 hit.
InterProiIPR003205. Cyt_c_oxidase_su8.
IPR016070. Cyt_c_oxidase_su8/PSI_RC_su9.
[Graphical view]
PANTHERiPTHR16717. PTHR16717. 1 hit.
PfamiPF02285. COX8. 1 hit.
[Graphical view]
SUPFAMiSSF81431. SSF81431. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRLAPTVRL LQAPLRGWAV PKAHITAKPA KTPTSPKEQA IGLSVTFLSF
60 70
LLPAGWVLYH LDNYKKSSAA
Length:70
Mass (Da):7,639
Last modified:November 1, 1995 - v3
Checksum:i17BE70ADEC45FDD8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191A → V in AAA30466. (PubMed:1689292)Curated
Sequence conflicti31 – 311K → R in AAA30466. (PubMed:1689292)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05202 mRNA. Translation: AAA30466.1.
U15540 Genomic DNA. Translation: AAA80279.1.
BC112717 mRNA. Translation: AAI12718.1.
PIRiA35537. OSBO8.
RefSeqiNP_001107989.1. NM_001114517.2.
UniGeneiBt.62953.

Genome annotation databases

EnsembliENSBTAT00000002651; ENSBTAP00000002651; ENSBTAG00000002046.
GeneIDi615757.
KEGGibta:615757.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05202 mRNA. Translation: AAA30466.1.
U15540 Genomic DNA. Translation: AAA80279.1.
BC112717 mRNA. Translation: AAI12718.1.
PIRiA35537. OSBO8.
RefSeqiNP_001107989.1. NM_001114517.2.
UniGeneiBt.62953.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OCCX-ray2.80M/Z25-70[»]
1OCOX-ray2.80M/Z25-70[»]
1OCRX-ray2.35M/Z25-70[»]
1OCZX-ray2.90M/Z25-70[»]
1V54X-ray1.80M/Z25-70[»]
1V55X-ray1.90M/Z25-70[»]
2DYRX-ray1.80M/Z25-70[»]
2DYSX-ray2.20M/Z25-70[»]
2EIJX-ray1.90M/Z25-70[»]
2EIKX-ray2.10M/Z25-70[»]
2EILX-ray2.10M/Z25-70[»]
2EIMX-ray2.60M/Z25-70[»]
2EINX-ray2.70M/Z25-70[»]
2OCCX-ray2.30M/Z25-70[»]
2Y69X-ray1.95M/Z1-70[»]
2YBBelectron microscopy19.00X25-70[»]
2ZXWX-ray2.50M/Z25-70[»]
3ABKX-ray2.00M/Z25-70[»]
3ABLX-ray2.10M/Z25-70[»]
3ABMX-ray1.95M/Z25-70[»]
3AG1X-ray2.20M/Z25-70[»]
3AG2X-ray1.80M/Z25-70[»]
3AG3X-ray1.80M/Z25-70[»]
3AG4X-ray2.05M/Z25-70[»]
3ASNX-ray3.00M/Z25-70[»]
3ASOX-ray2.30M/Z25-70[»]
3WG7X-ray1.90M/Z25-70[»]
ProteinModelPortaliP10175.
SMRiP10175. Positions 25-67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60941N.
STRINGi9913.ENSBTAP00000002651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002651; ENSBTAP00000002651; ENSBTAG00000002046.
GeneIDi615757.
KEGGibta:615757.

Organism-specific databases

CTDi12869.

Phylogenomic databases

eggNOGiNOG83531.
GeneTreeiENSGT00530000065013.
HOGENOMiHOG000230478.
InParanoidiP10175.
KOiK02273.
OrthoDBiEOG769ZNX.
TreeFamiTF105070.

Miscellaneous databases

EvolutionaryTraceiP10175.
NextBioi20899771.

Family and domain databases

Gene3Di4.10.81.10. 1 hit.
InterProiIPR003205. Cyt_c_oxidase_su8.
IPR016070. Cyt_c_oxidase_su8/PSI_RC_su9.
[Graphical view]
PANTHERiPTHR16717. PTHR16717. 1 hit.
PfamiPF02285. COX8. 1 hit.
[Graphical view]
SUPFAMiSSF81431. SSF81431. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the cDNAs encoding two isoforms of subunit CIX of bovine cytochrome c oxidase."
    Lightowlers R.N., Ewart G.D., Aggeler R.J., Zhang Y.-Z., Calavetta L., Capaldi R.A.
    J. Biol. Chem. 265:2677-2681(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. "Structure and chromosomal location of the bovine gene for the heart muscle isoform of cytochrome c oxidase subunit VIII."
    Lomax M.I., Riggs P.K., Womack J.E.
    Mamm. Genome 6:118-122(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Rumen.
  4. "Studies on cytochrome c oxidase, X. Isolation and amino-acid sequence of polypeptide VIIIb."
    Meinecke L., Steffens G.J., Buse G.
    Hoppe-Seyler's Z. Physiol. Chem. 365:313-320(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-70.
    Tissue: Heart.
  5. "Different isozymes of cytochrome c oxidase are expressed in bovine smooth muscle and skeletal or heart muscle."
    Anthony G., Stroh A., Lottspeich F., Kadenbach B.
    FEBS Lett. 277:97-100(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-30.
    Tissue: Heart.
  6. "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A."
    Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H., Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.
    Science 272:1136-1144(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 25-70.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 25-70.
    Tissue: Heart.
  8. "X-ray structure of azide-bound fully oxidized cytochrome c oxidase from bovine heart at 2.9 A resolution."
    Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.
    Acta Crystallogr. D 56:529-535(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 25-70.
    Tissue: Heart.

Entry informationi

Entry nameiCOX8B_BOVIN
AccessioniPrimary (citable) accession number: P10175
Secondary accession number(s): Q2KI98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.