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P10175

- COX8B_BOVIN

UniProt

P10175 - COX8B_BOVIN

Protein

Cytochrome c oxidase subunit 8B, mitochondrial

Gene

COX8B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 8B, mitochondrial
    Alternative name(s):
    Cytochrome c oxidase polypeptide VIII-heart
    Cytochrome c oxidase subunit 8-1
    Cytochrome c oxidase subunit 8H
    IX
    VIIIb
    Gene namesi
    Name:COX8B
    Synonyms:COX8H
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 11

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. respiratory chain complex IV Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2424Mitochondrion2 PublicationsAdd
    BLAST
    Chaini25 – 7046Cytochrome c oxidase subunit 8B, mitochondrialPRO_0000006176Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60941N.
    STRINGi9913.ENSBTAP00000002651.

    Structurei

    Secondary structure

    1
    70
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi36 – 5924
    Helixi61 – 655

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OCCX-ray2.80M/Z25-70[»]
    1OCOX-ray2.80M/Z25-70[»]
    1OCRX-ray2.35M/Z25-70[»]
    1OCZX-ray2.90M/Z25-70[»]
    1V54X-ray1.80M/Z25-70[»]
    1V55X-ray1.90M/Z25-70[»]
    2DYRX-ray1.80M/Z25-70[»]
    2DYSX-ray2.20M/Z25-70[»]
    2EIJX-ray1.90M/Z25-70[»]
    2EIKX-ray2.10M/Z25-70[»]
    2EILX-ray2.10M/Z25-70[»]
    2EIMX-ray2.60M/Z25-70[»]
    2EINX-ray2.70M/Z25-70[»]
    2OCCX-ray2.30M/Z25-70[»]
    2Y69X-ray1.95M/Z1-70[»]
    2YBBelectron microscopy19.00X25-70[»]
    2ZXWX-ray2.50M/Z25-70[»]
    3ABKX-ray2.00M/Z25-70[»]
    3ABLX-ray2.10M/Z25-70[»]
    3ABMX-ray1.95M/Z25-70[»]
    3AG1X-ray2.20M/Z25-70[»]
    3AG2X-ray1.80M/Z25-70[»]
    3AG3X-ray1.80M/Z25-70[»]
    3AG4X-ray2.05M/Z25-70[»]
    3ASNX-ray3.00M/Z25-70[»]
    3ASOX-ray2.30M/Z25-70[»]
    3WG7X-ray1.90M/Z25-70[»]
    ProteinModelPortaliP10175.
    SMRiP10175. Positions 25-67.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10175.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 3511Mitochondrial matrixAdd
    BLAST
    Topological domaini60 – 7011Mitochondrial intermembraneAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5924HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome c oxidase VIII family.Curated

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG83531.
    GeneTreeiENSGT00530000065013.
    HOGENOMiHOG000230478.
    InParanoidiP10175.
    KOiK02273.
    OrthoDBiEOG769ZNX.
    TreeFamiTF105070.

    Family and domain databases

    Gene3Di4.10.81.10. 1 hit.
    InterProiIPR003205. Cyt_c_oxidase_su8.
    IPR016070. Cyt_c_oxidase_su8/PSI_RC_su9.
    [Graphical view]
    PANTHERiPTHR16717. PTHR16717. 1 hit.
    PfamiPF02285. COX8. 1 hit.
    [Graphical view]
    SUPFAMiSSF81431. SSF81431. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10175-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRLAPTVRL LQAPLRGWAV PKAHITAKPA KTPTSPKEQA IGLSVTFLSF   50
    LLPAGWVLYH LDNYKKSSAA 70
    Length:70
    Mass (Da):7,639
    Last modified:November 1, 1995 - v3
    Checksum:i17BE70ADEC45FDD8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191A → V in AAA30466. (PubMed:1689292)Curated
    Sequence conflicti31 – 311K → R in AAA30466. (PubMed:1689292)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05202 mRNA. Translation: AAA30466.1.
    U15540 Genomic DNA. Translation: AAA80279.1.
    BC112717 mRNA. Translation: AAI12718.1.
    PIRiA35537. OSBO8.
    RefSeqiNP_001107989.1. NM_001114517.2.
    UniGeneiBt.62953.

    Genome annotation databases

    EnsembliENSBTAT00000002651; ENSBTAP00000002651; ENSBTAG00000002046.
    GeneIDi615757.
    KEGGibta:615757.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05202 mRNA. Translation: AAA30466.1 .
    U15540 Genomic DNA. Translation: AAA80279.1 .
    BC112717 mRNA. Translation: AAI12718.1 .
    PIRi A35537. OSBO8.
    RefSeqi NP_001107989.1. NM_001114517.2.
    UniGenei Bt.62953.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OCC X-ray 2.80 M/Z 25-70 [» ]
    1OCO X-ray 2.80 M/Z 25-70 [» ]
    1OCR X-ray 2.35 M/Z 25-70 [» ]
    1OCZ X-ray 2.90 M/Z 25-70 [» ]
    1V54 X-ray 1.80 M/Z 25-70 [» ]
    1V55 X-ray 1.90 M/Z 25-70 [» ]
    2DYR X-ray 1.80 M/Z 25-70 [» ]
    2DYS X-ray 2.20 M/Z 25-70 [» ]
    2EIJ X-ray 1.90 M/Z 25-70 [» ]
    2EIK X-ray 2.10 M/Z 25-70 [» ]
    2EIL X-ray 2.10 M/Z 25-70 [» ]
    2EIM X-ray 2.60 M/Z 25-70 [» ]
    2EIN X-ray 2.70 M/Z 25-70 [» ]
    2OCC X-ray 2.30 M/Z 25-70 [» ]
    2Y69 X-ray 1.95 M/Z 1-70 [» ]
    2YBB electron microscopy 19.00 X 25-70 [» ]
    2ZXW X-ray 2.50 M/Z 25-70 [» ]
    3ABK X-ray 2.00 M/Z 25-70 [» ]
    3ABL X-ray 2.10 M/Z 25-70 [» ]
    3ABM X-ray 1.95 M/Z 25-70 [» ]
    3AG1 X-ray 2.20 M/Z 25-70 [» ]
    3AG2 X-ray 1.80 M/Z 25-70 [» ]
    3AG3 X-ray 1.80 M/Z 25-70 [» ]
    3AG4 X-ray 2.05 M/Z 25-70 [» ]
    3ASN X-ray 3.00 M/Z 25-70 [» ]
    3ASO X-ray 2.30 M/Z 25-70 [» ]
    3WG7 X-ray 1.90 M/Z 25-70 [» ]
    ProteinModelPortali P10175.
    SMRi P10175. Positions 25-67.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60941N.
    STRINGi 9913.ENSBTAP00000002651.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000002651 ; ENSBTAP00000002651 ; ENSBTAG00000002046 .
    GeneIDi 615757.
    KEGGi bta:615757.

    Organism-specific databases

    CTDi 12869.

    Phylogenomic databases

    eggNOGi NOG83531.
    GeneTreei ENSGT00530000065013.
    HOGENOMi HOG000230478.
    InParanoidi P10175.
    KOi K02273.
    OrthoDBi EOG769ZNX.
    TreeFami TF105070.

    Miscellaneous databases

    EvolutionaryTracei P10175.
    NextBioi 20899771.

    Family and domain databases

    Gene3Di 4.10.81.10. 1 hit.
    InterProi IPR003205. Cyt_c_oxidase_su8.
    IPR016070. Cyt_c_oxidase_su8/PSI_RC_su9.
    [Graphical view ]
    PANTHERi PTHR16717. PTHR16717. 1 hit.
    Pfami PF02285. COX8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81431. SSF81431. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the cDNAs encoding two isoforms of subunit CIX of bovine cytochrome c oxidase."
      Lightowlers R.N., Ewart G.D., Aggeler R.J., Zhang Y.-Z., Calavetta L., Capaldi R.A.
      J. Biol. Chem. 265:2677-2681(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. "Structure and chromosomal location of the bovine gene for the heart muscle isoform of cytochrome c oxidase subunit VIII."
      Lomax M.I., Riggs P.K., Womack J.E.
      Mamm. Genome 6:118-122(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Rumen.
    4. "Studies on cytochrome c oxidase, X. Isolation and amino-acid sequence of polypeptide VIIIb."
      Meinecke L., Steffens G.J., Buse G.
      Hoppe-Seyler's Z. Physiol. Chem. 365:313-320(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-70.
      Tissue: Heart.
    5. "Different isozymes of cytochrome c oxidase are expressed in bovine smooth muscle and skeletal or heart muscle."
      Anthony G., Stroh A., Lottspeich F., Kadenbach B.
      FEBS Lett. 277:97-100(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-30.
      Tissue: Heart.
    6. "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A."
      Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H., Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.
      Science 272:1136-1144(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 25-70.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 25-70.
      Tissue: Heart.
    8. "X-ray structure of azide-bound fully oxidized cytochrome c oxidase from bovine heart at 2.9 A resolution."
      Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.
      Acta Crystallogr. D 56:529-535(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 25-70.
      Tissue: Heart.

    Entry informationi

    Entry nameiCOX8B_BOVIN
    AccessioniPrimary (citable) accession number: P10175
    Secondary accession number(s): Q2KI98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3