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P10163 (PRB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Basic salivary proline-rich protein 4

Short name=Salivary proline-rich protein Po
Alternative name(s):
Parotid o protein
Salivary proline-rich protein II-1

Cleaved into the following 3 chains:

  1. Protein N1
  2. Glycosylated protein A
  3. Peptide P-D
    Alternative name(s):
    Proline-rich peptide IB-5
Gene names
Name:PRB4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secreted.

Post-translational modification

N-glycosylated. Ref.11

Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P3 position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation found on at least Gln-46, Gln-48, Gln-67, Gln-88; Gln-90; Gln-193; Gln-288 Gln-214 and Gln-295, preferentially in diabetic, and head and neck cancer patients. Ref.10

Polymorphism

The number of repeats is polymorphic and varies among different alleles. Allele S (short), allele M (medium) and allele L (long) contain 6, 7 and 9 tandem repeats respectively.

Sequence caution

The sequence CAA30543.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA30729.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainRepeat
Signal
   PTMGlycoprotein
Pyrrolidone carboxylic acid
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Non-traceable author statement Ref.5. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Ref.5
Chain17 – 310294Basic salivary proline-rich protein 4
PRO_0000022102
Peptide17 – 3923Protein N1
PRO_0000022103
Chain40 – 177138Glycosylated protein A
PRO_0000022104
Chain241 – 31070Peptide P-D
PRO_0000022099

Regions

Repeat35 – 55211
Repeat56 – 76212
Repeat77 – 97213
Repeat98 – 118214
Repeat119 – 139215
Repeat140 – 160216
Repeat161 – 181217
Repeat182 – 202218
Repeat203 – 223219
Repeat224 – 2341110; truncated
Region35 – 2342009.5 X 21 AA tandem repeats of K-P-[EQ]-[GR]-[PR]-[PR]-P-Q-G-G-N-Q-[PS]-[QH]-[RG]-[PT]-P-P-[PH]-P-G

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation871N-linked (GlcNAc...) Ref.11
Glycosylation1081N-linked (GlcNAc...) Potential
Glycosylation1501N-linked (GlcNAc...) Potential
Glycosylation1711N-linked (GlcNAc...) Potential
Glycosylation1921N-linked (GlcNAc...) Potential
Glycosylation2131N-linked (GlcNAc...) Potential
Glycosylation2341N-linked (GlcNAc...) Potential

Natural variations

Natural variant113 – 15442Missing in allele M and allele S.
VAR_035034
Natural variant164 – 18421Missing in allele S.
VAR_035035
Natural variant1851R → G.
Corresponds to variant rs11054244 [ dbSNP | Ensembl ].
VAR_031548
Natural variant1861P → R.
Corresponds to variant rs11054243 [ dbSNP | Ensembl ].
VAR_031549
Natural variant2001P → H.
Corresponds to variant rs12308244 [ dbSNP | Ensembl ].
VAR_031550
Natural variant2721A → P. Ref.4 Ref.6 Ref.7
Corresponds to variant rs1052808 [ dbSNP | Ensembl ].
VAR_031551

Experimental info

Sequence conflict281S → P AA sequence Ref.5
Sequence conflict31 – 399LISGKPEGR → IIPPKPPG AA sequence Ref.5
Sequence conflict31 – 333LIS → PPP in AAB50687. Ref.6
Sequence conflict371E → Q in CAA30543. Ref.2
Sequence conflict371E → Q in CAA30542. Ref.7
Sequence conflict661N → D AA sequence Ref.5
Sequence conflict74 – 9421Missing in CAA30542. Ref.7
Sequence conflict961P → PP AA sequence Ref.5
Sequence conflict1011R → E AA sequence Ref.5
Sequence conflict122 – 1232SR → RP in CAA30542. Ref.7
Sequence conflict1291H → N in CAA30542. Ref.7
Sequence conflict154 – 17421Missing in CAA30542. Ref.7
Sequence conflict169 – 1713GGN → QGG AA sequence Ref.5
Sequence conflict1921N → D AA sequence Ref.5
Sequence conflict2131N → D AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P10163 [UniParc].

Last modified May 14, 2014. Version 4.
Checksum: 079538A1BC412D0F

FASTA31031,326
        10         20         30         40         50         60 
MLLILLSVAL LALSSAESSS EDVSQEESLF LISGKPEGRR PQGGNQPQRP PPPPGKPQGP 

        70         80         90        100        110        120 
PPQGGNQSQG PPPPPGKPEG RPPQGGNQSQ GPPPHPGKPE RPPPQGGNQS QGPPPHPGKP 

       130        140        150        160        170        180 
ESRPPQGGHQ SQGPPPTPGK PEGPPPQGGN QSQGTPPPPG KPEGRPPQGG NQSQGPPPHP 

       190        200        210        220        230        240 
GKPERPPPQG GNQSHRPPPP PGKPERPPPQ GGNQSQGPPP HPGKPEGPPP QEGNKSRSAR 

       250        260        270        280        290        300 
SPPGKPQGPP QQEGNKPQGP PPPGKPQGPP PAGGNPQQPQ APPAGKPQGP PPPPQGGRPP 

       310 
RPAQGQQPPQ 

« Hide

References

« Hide 'large scale' references
[1]"Differential RNA splicing and post-translational cleavages in the human salivary proline-rich protein gene system."
Maeda N., Kim H.-S., Azen E.A., Smithies O.
J. Biol. Chem. 260:11123-11130(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE S), POLYMORPHISM.
[2]"Length polymorphisms in human proline-rich protein genes generated by intragenic unequal crossing over."
Lyons K.M., Stein J.H., Smithies O.
Genetics 120:267-278(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES L AND S).
[3]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE M), VARIANT PRO-272.
Tissue: Cerebellum.
[5]"Alignment of amino acid and DNA sequences of human proline-rich proteins."
Kauffman D.L., Keller P.J., Bennick A., Blum M.
Crit. Rev. Oral Biol. Med. 4:287-292(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 17-112 AND 155-240.
Tissue: Saliva.
[6]"PRB1, PRB2, and PRB4 coded polymorphisms among human salivary concanavalin-A binding, II-1, and Po proline-rich proteins."
Azen E.A., Amberger E., Fisher S., Prakobphol A., Niece R.L.
Am. J. Hum. Genet. 58:143-153(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-310 (ALLELE M), VARIANT PRO-272.
[7]"Many protein products from a few loci: assignment of human salivary proline-rich proteins to specific loci."
Lyons K.M., Stein J.H., Smithies O.
Genetics 120:255-265(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-310, VARIANT PRO-272.
[8]"Complete amino acid sequence of a basic proline-rich peptide, P-D, from human parotid saliva."
Saitoh E., Isemura S., Sanada K.
J. Biochem. 93:495-502(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 241-310.
Tissue: Saliva.
[9]"Basic proline-rich proteins from human parotid saliva: relationships of the covalent structures of ten proteins from a single individual."
Kauffman D.L., Bennick A., Blum M., Keller P.J.
Biochemistry 30:3351-3356(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 241-310.
Tissue: Saliva.
[10]"Identification of Lys-Pro-Gln as a novel cleavage site specificity of saliva-associated proteases."
Helmerhorst E.J., Sun X., Salih E., Oppenheim F.G.
J. Biol. Chem. 283:19957-19966(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING, IDENTIFICATION BY MASS SPECTROMETRY.
[11]"Finding new posttranslational modifications in salivary proline-rich proteins."
Vitorino R., Alves R., Barros A., Caseiro A., Ferreira R., Lobo M.C., Bastos A., Duarte J., Carvalho D., Santos L.L., Amado F.L.
Proteomics 10:3732-3742(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-87, PYROGLUTAMATE FORMATION, VARIANTS ALLELE L AND M, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Web resources

SHMPD

The Singapore human mutation and polymorphism database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K03207 mRNA. Translation: AAA60188.1.
X07882 Genomic DNA. Translation: CAA30729.1. Sequence problems.
X07715 Genomic DNA. Translation: CAA30543.1. Sequence problems.
AC010176 Genomic DNA. No translation available.
BC130386 mRNA. Translation: AAI30387.1.
S80916 Genomic DNA. Translation: AAB50687.2.
X07704 Genomic DNA. Translation: CAA30542.1.
PIRPIHUSD. S03176.
RefSeqNP_001248328.1. NM_001261399.1.
NP_002714.2. NM_002723.4.
UniGeneHs.528651.

3D structure databases

DisProtDP00119.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000279575.

Polymorphism databases

DMDM158517854.

Proteomic databases

PaxDbP10163.
PRIDEP10163.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5545.
KEGGhsa:5545.

Organism-specific databases

CTD5545.
GeneCardsGC12M011460.
H-InvDBHIX0079490.
HGNCHGNC:9340. PRB4.
MIM180990. gene.
neXtProtNX_P10163.
PharmGKBPA33702.
GenAtlasSearch...

Gene expression databases

CleanExHS_PRB4.
GenevestigatorP10163.

Family and domain databases

InterProIPR026086. Pro-rich.
[Graphical view]
PANTHERPTHR23203. PTHR23203. 1 hit.
PfamPF15240. Pro-rich. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPRB4.
GenomeRNAi5545.
NextBio21484.
PROP10163.
SOURCESearch...

Entry information

Entry namePRB4_HUMAN
AccessionPrimary (citable) accession number: P10163
Secondary accession number(s): A1L439 expand/collapse secondary AC list , O00600, P02813, P10161, P10162, P81489
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 14, 2014
Last modified: June 11, 2014
This is version 102 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM