Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P10163

- PRB4_HUMAN

UniProt

P10163 - PRB4_HUMAN

Protein

Basic salivary proline-rich protein 4

Gene

PRB4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 4 (14 May 2014)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Basic salivary proline-rich protein 4
    Short name:
    Salivary proline-rich protein Po
    Alternative name(s):
    Parotid o protein
    Salivary proline-rich protein II-1
    Cleaved into the following 3 chains:
    Alternative name(s):
    Proline-rich peptide IB-5
    Gene namesi
    Name:PRB4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Unplaced

    Organism-specific databases

    HGNCiHGNC:9340. PRB4.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33702.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 16161 PublicationAdd
    BLAST
    Chaini17 – 310294Basic salivary proline-rich protein 4PRO_0000022102Add
    BLAST
    Peptidei17 – 3923Protein N1PRO_0000022103Add
    BLAST
    Chaini40 – 177138Glycosylated protein APRO_0000022104Add
    BLAST
    Chaini241 – 31070Peptide P-DPRO_0000022099Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi87 – 871N-linked (GlcNAc...)1 Publication
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication
    Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P3 position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation found on at least Gln-46, Gln-48, Gln-67, Gln-88; Gln-90; Gln-193; Gln-288 Gln-214 and Gln-295, preferentially in diabetic, and head and neck cancer patients.1 Publication

    Keywords - PTMi

    Glycoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP10163.
    PRIDEiP10163.

    Expressioni

    Gene expression databases

    CleanExiHS_PRB4.
    GenevestigatoriP10163.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000279575.

    Structurei

    3D structure databases

    DisProtiDP00119.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati35 – 55211Add
    BLAST
    Repeati56 – 76212Add
    BLAST
    Repeati77 – 97213Add
    BLAST
    Repeati98 – 118214Add
    BLAST
    Repeati119 – 139215Add
    BLAST
    Repeati140 – 160216Add
    BLAST
    Repeati161 – 181217Add
    BLAST
    Repeati182 – 202218Add
    BLAST
    Repeati203 – 223219Add
    BLAST
    Repeati224 – 2341110; truncatedAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni35 – 2342009.5 X 21 AA tandem repeats of K-P-[EQ]-[GR]-[PR]-[PR]-P-Q-G-G-N-Q-[PS]-[QH]-[RG]-[PT]-P-P-[PH]-P-GAdd
    BLAST

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    InterProiIPR026086. Pro-rich.
    [Graphical view]
    PANTHERiPTHR23203. PTHR23203. 1 hit.
    PfamiPF15240. Pro-rich. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10163-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLILLSVAL LALSSAESSS EDVSQEESLF LISGKPEGRR PQGGNQPQRP    50
    PPPPGKPQGP PPQGGNQSQG PPPPPGKPEG RPPQGGNQSQ GPPPHPGKPE 100
    RPPPQGGNQS QGPPPHPGKP ESRPPQGGHQ SQGPPPTPGK PEGPPPQGGN 150
    QSQGTPPPPG KPEGRPPQGG NQSQGPPPHP GKPERPPPQG GNQSHRPPPP 200
    PGKPERPPPQ GGNQSQGPPP HPGKPEGPPP QEGNKSRSAR SPPGKPQGPP 250
    QQEGNKPQGP PPPGKPQGPP PAGGNPQQPQ APPAGKPQGP PPPPQGGRPP 300
    RPAQGQQPPQ 310
    Length:310
    Mass (Da):31,326
    Last modified:May 14, 2014 - v4
    Checksum:i079538A1BC412D0F
    GO

    Sequence cautioni

    The sequence CAA30543.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA30729.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281S → P AA sequence (PubMed:8373986)Curated
    Sequence conflicti31 – 399LISGKPEGR → IIPPKPPG AA sequence (PubMed:8373986)Curated
    Sequence conflicti31 – 333LIS → PPP in AAB50687. (PubMed:8554050)Curated
    Sequence conflicti37 – 371E → Q in CAA30543. (PubMed:2851479)Curated
    Sequence conflicti37 – 371E → Q in CAA30542. (PubMed:3220251)Curated
    Sequence conflicti66 – 661N → D AA sequence (PubMed:8373986)Curated
    Sequence conflicti74 – 9421Missing in CAA30542. (PubMed:3220251)CuratedAdd
    BLAST
    Sequence conflicti96 – 961P → PP AA sequence (PubMed:8373986)Curated
    Sequence conflicti101 – 1011R → E AA sequence (PubMed:8373986)Curated
    Sequence conflicti122 – 1232SR → RP in CAA30542. (PubMed:3220251)Curated
    Sequence conflicti129 – 1291H → N in CAA30542. (PubMed:3220251)Curated
    Sequence conflicti154 – 17421Missing in CAA30542. (PubMed:3220251)CuratedAdd
    BLAST
    Sequence conflicti169 – 1713GGN → QGG AA sequence (PubMed:8373986)Curated
    Sequence conflicti192 – 1921N → D AA sequence (PubMed:8373986)Curated
    Sequence conflicti213 – 2131N → D AA sequence (PubMed:8373986)Curated

    Polymorphismi

    The number of repeats is polymorphic and varies among different alleles. Allele S (short), allele M (medium) and allele L (long) contain 6, 7 and 9 tandem repeats respectively.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti113 – 15442Missing in allele M and allele S.
    VAR_035034Add
    BLAST
    Natural varianti164 – 18421Missing in allele S.
    VAR_035035Add
    BLAST
    Natural varianti185 – 1851R → G.
    Corresponds to variant rs11054244 [ dbSNP | Ensembl ].
    VAR_031548
    Natural varianti186 – 1861P → R.
    Corresponds to variant rs11054243 [ dbSNP | Ensembl ].
    VAR_031549
    Natural varianti200 – 2001P → H.
    Corresponds to variant rs12308244 [ dbSNP | Ensembl ].
    VAR_031550
    Natural varianti272 – 2721A → P.3 Publications
    Corresponds to variant rs1052808 [ dbSNP | Ensembl ].
    VAR_031551

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03207 mRNA. Translation: AAA60188.1.
    X07882 Genomic DNA. Translation: CAA30729.1. Sequence problems.
    X07715 Genomic DNA. Translation: CAA30543.1. Sequence problems.
    AC010176 Genomic DNA. No translation available.
    BC130386 mRNA. Translation: AAI30387.1.
    S80916 Genomic DNA. Translation: AAB50687.2.
    X07704 Genomic DNA. Translation: CAA30542.1.
    PIRiS03176. PIHUSD.
    RefSeqiNP_001248328.1. NM_001261399.1.
    NP_002714.2. NM_002723.4.
    UniGeneiHs.528651.

    Genome annotation databases

    GeneIDi5545.
    KEGGihsa:5545.

    Polymorphism databases

    DMDMi158517854.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    SHMPD

    The Singapore human mutation and polymorphism database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03207 mRNA. Translation: AAA60188.1 .
    X07882 Genomic DNA. Translation: CAA30729.1 . Sequence problems.
    X07715 Genomic DNA. Translation: CAA30543.1 . Sequence problems.
    AC010176 Genomic DNA. No translation available.
    BC130386 mRNA. Translation: AAI30387.1 .
    S80916 Genomic DNA. Translation: AAB50687.2 .
    X07704 Genomic DNA. Translation: CAA30542.1 .
    PIRi S03176. PIHUSD.
    RefSeqi NP_001248328.1. NM_001261399.1.
    NP_002714.2. NM_002723.4.
    UniGenei Hs.528651.

    3D structure databases

    DisProti DP00119.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000279575.

    Polymorphism databases

    DMDMi 158517854.

    Proteomic databases

    PaxDbi P10163.
    PRIDEi P10163.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5545.
    KEGGi hsa:5545.

    Organism-specific databases

    CTDi 5545.
    GeneCardsi GC12M011460.
    H-InvDB HIX0079490.
    HGNCi HGNC:9340. PRB4.
    MIMi 180990. gene.
    neXtProti NX_P10163.
    PharmGKBi PA33702.
    GenAtlasi Search...

    Miscellaneous databases

    GeneWikii PRB4.
    GenomeRNAii 5545.
    NextBioi 21484.
    PROi P10163.
    SOURCEi Search...

    Gene expression databases

    CleanExi HS_PRB4.
    Genevestigatori P10163.

    Family and domain databases

    InterProi IPR026086. Pro-rich.
    [Graphical view ]
    PANTHERi PTHR23203. PTHR23203. 1 hit.
    Pfami PF15240. Pro-rich. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential RNA splicing and post-translational cleavages in the human salivary proline-rich protein gene system."
      Maeda N., Kim H.-S., Azen E.A., Smithies O.
      J. Biol. Chem. 260:11123-11130(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE S), POLYMORPHISM.
    2. "Length polymorphisms in human proline-rich protein genes generated by intragenic unequal crossing over."
      Lyons K.M., Stein J.H., Smithies O.
      Genetics 120:267-278(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES L AND S).
    3. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE M), VARIANT PRO-272.
      Tissue: Cerebellum.
    5. "Alignment of amino acid and DNA sequences of human proline-rich proteins."
      Kauffman D.L., Keller P.J., Bennick A., Blum M.
      Crit. Rev. Oral Biol. Med. 4:287-292(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 17-112 AND 155-240.
      Tissue: Saliva.
    6. "PRB1, PRB2, and PRB4 coded polymorphisms among human salivary concanavalin-A binding, II-1, and Po proline-rich proteins."
      Azen E.A., Amberger E., Fisher S., Prakobphol A., Niece R.L.
      Am. J. Hum. Genet. 58:143-153(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-310 (ALLELE M), VARIANT PRO-272.
    7. "Many protein products from a few loci: assignment of human salivary proline-rich proteins to specific loci."
      Lyons K.M., Stein J.H., Smithies O.
      Genetics 120:255-265(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-310, VARIANT PRO-272.
    8. "Complete amino acid sequence of a basic proline-rich peptide, P-D, from human parotid saliva."
      Saitoh E., Isemura S., Sanada K.
      J. Biochem. 93:495-502(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 241-310.
      Tissue: Saliva.
    9. "Basic proline-rich proteins from human parotid saliva: relationships of the covalent structures of ten proteins from a single individual."
      Kauffman D.L., Bennick A., Blum M., Keller P.J.
      Biochemistry 30:3351-3356(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 241-310.
      Tissue: Saliva.
    10. "Identification of Lys-Pro-Gln as a novel cleavage site specificity of saliva-associated proteases."
      Helmerhorst E.J., Sun X., Salih E., Oppenheim F.G.
      J. Biol. Chem. 283:19957-19966(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING, IDENTIFICATION BY MASS SPECTROMETRY.
    11. "Finding new posttranslational modifications in salivary proline-rich proteins."
      Vitorino R., Alves R., Barros A., Caseiro A., Ferreira R., Lobo M.C., Bastos A., Duarte J., Carvalho D., Santos L.L., Amado F.L.
      Proteomics 10:3732-3742(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-87, PYROGLUTAMATE FORMATION, VARIANTS ALLELE L AND M, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPRB4_HUMAN
    AccessioniPrimary (citable) accession number: P10163
    Secondary accession number(s): A1L439
    , O00600, P02813, P10161, P10162, P81489
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: May 14, 2014
    Last modified: October 1, 2014
    This is version 103 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3