P10163 (PRB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Basic salivary proline-rich protein 4
Short name=Salivary proline-rich protein Po

Alternative name(s):
Parotid o protein
Salivary proline-rich protein II-1

Cleaved into the following 3 chains:

1. Protein N1
2. Glycosylated protein A
3. Peptide P-D
   Alternative name(s):
   Proline-rich peptide IB-5

Gene names

Name:

PRB4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Post-translational modification	N-glycosylated. Ref.11 Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P3 position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation found on at least Gln-46, Gln-48, Gln-67, Gln-88; Gln-90; Gln-193; Gln-288 Gln-214 and Gln-295, preferentially in diabetic, and head and neck cancer patients. Ref.10
Polymorphism	The number of repeats is polymorphic and varies among different alleles. Allele S (short), allele M (medium) and allele L (long) contain 6, 7 and 9 tandem repeats respectively.
Sequence caution	The sequence CAA30543.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA30729.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Repeat Signal
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Non-traceable author statement Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.5
	Chain	17 – 310	294	Basic salivary proline-rich protein 4		PRO_0000022102
	Peptide	17 – 39	23	Protein N1		PRO_0000022103
	Chain	40 – 177	138	Glycosylated protein A		PRO_0000022104
	Chain	241 – 310	70	Peptide P-D		PRO_0000022099
Regions
	Repeat	35 – 55	21	1
	Repeat	56 – 76	21	2
	Repeat	77 – 97	21	3
	Repeat	98 – 118	21	4
	Repeat	119 – 139	21	5
	Repeat	140 – 160	21	6
	Repeat	161 – 181	21	7
	Repeat	182 – 202	21	8
	Repeat	203 – 223	21	9
	Repeat	224 – 234	11	10; truncated
	Region	35 – 234	200	9.5 X 21 AA tandem repeats of K-P-[EQ]-[GR]-[PR]-[PR]-P-Q-G-G-N-Q-[PS]-[QH]-[RG]-[PT]-P-P-[PH]-P-G
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	87	1	N-linked (GlcNAc...) Ref.11
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Glycosylation	150	1	N-linked (GlcNAc...) Potential
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	234	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	113 – 154	42	Missing in allele M and allele S.		VAR_035034
	Natural variant	164 – 184	21	Missing in allele S.		VAR_035035
	Natural variant	185	1	R → G. Corresponds to variant rs11054244 [ dbSNP | Ensembl ].		VAR_031548
	Natural variant	186	1	P → R. Corresponds to variant rs11054243 [ dbSNP | Ensembl ].		VAR_031549
	Natural variant	200	1	P → H. Corresponds to variant rs12308244 [ dbSNP | Ensembl ].		VAR_031550
	Natural variant	272	1	P → A. Ref.1 Ref.2 Ref.3 Corresponds to variant rs1052808 [ dbSNP | Ensembl ].		VAR_031551
Experimental info
	Sequence conflict	28	1	S → P AA sequence Ref.5
	Sequence conflict	31 – 39	9	LISGKPEGR → IIPPKPPG AA sequence Ref.5
	Sequence conflict	31 – 33	3	LIS → PPP in AAB50687. Ref.6
	Sequence conflict	37	1	E → Q in CAA30543. Ref.2
	Sequence conflict	37	1	E → Q in CAA30542. Ref.7
	Sequence conflict	66	1	N → D AA sequence Ref.5
	Sequence conflict	74 – 94	21	Missing in CAA30542. Ref.7
	Sequence conflict	96	1	P → PP AA sequence Ref.5
	Sequence conflict	101	1	R → E AA sequence Ref.5
	Sequence conflict	122 – 123	2	SR → RP in CAA30542. Ref.7
	Sequence conflict	129	1	H → N in CAA30542. Ref.7
	Sequence conflict	154 – 174	21	Missing in CAA30542. Ref.7
	Sequence conflict	169 – 171	3	GGN → QGG AA sequence Ref.5
	Sequence conflict	192	1	N → D AA sequence Ref.5
	Sequence conflict	213	1	N → D AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P10163 [UniParc]. Last modified September 11, 2007. Version 3. Checksum: F3D038A1BC413615	FASTA	310	31,352
	10 20 30 40 50 60 MLLILLSVAL LALSSAESSS EDVSQEESLF LISGKPEGRR PQGGNQPQRP PPPPGKPQGP 70 80 90 100 110 120 PPQGGNQSQG PPPPPGKPEG RPPQGGNQSQ GPPPHPGKPE RPPPQGGNQS QGPPPHPGKP 130 140 150 160 170 180 ESRPPQGGHQ SQGPPPTPGK PEGPPPQGGN QSQGTPPPPG KPEGRPPQGG NQSQGPPPHP 190 200 210 220 230 240 GKPERPPPQG GNQSHRPPPP PGKPERPPPQ GGNQSQGPPP HPGKPEGPPP QEGNKSRSAR 250 260 270 280 290 300 SPPGKPQGPP QQEGNKPQGP PPPGKPQGPP PPGGNPQQPQ APPAGKPQGP PPPPQGGRPP 310 RPAQGQQPPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential RNA splicing and post-translational cleavages in the human salivary proline-rich protein gene system." Maeda N., Kim H.-S., Azen E.A., Smithies O. J. Biol. Chem. 260:11123-11130(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE S), VARIANT ALA-272, POLYMORPHISM.
[2]	"Length polymorphisms in human proline-rich protein genes generated by intragenic unequal crossing over." Lyons K.M., Stein J.H., Smithies O. Genetics 120:267-278(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES L AND S), VARIANT ALA-272.
[3]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-272.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE M). Tissue: Cerebellum.
[5]	"Alignment of amino acid and DNA sequences of human proline-rich proteins." Kauffman D.L., Keller P.J., Bennick A., Blum M. Crit. Rev. Oral Biol. Med. 4:287-292(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-112 AND 155-240. Tissue: Saliva.
[6]	"PRB1, PRB2, and PRB4 coded polymorphisms among human salivary concanavalin-A binding, II-1, and Po proline-rich proteins." Azen E.A., Amberger E., Fisher S., Prakobphol A., Niece R.L. Am. J. Hum. Genet. 58:143-153(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-310 (ALLELE M).
[7]	"Many protein products from a few loci: assignment of human salivary proline-rich proteins to specific loci." Lyons K.M., Stein J.H., Smithies O. Genetics 120:255-265(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-310.
[8]	"Complete amino acid sequence of a basic proline-rich peptide, P-D, from human parotid saliva." Saitoh E., Isemura S., Sanada K. J. Biochem. 93:495-502(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 241-310. Tissue: Saliva.
[9]	"Basic proline-rich proteins from human parotid saliva: relationships of the covalent structures of ten proteins from a single individual." Kauffman D.L., Bennick A., Blum M., Keller P.J. Biochemistry 30:3351-3356(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 241-310. Tissue: Saliva.
[10]	"Identification of Lys-Pro-Gln as a novel cleavage site specificity of saliva-associated proteases." Helmerhorst E.J., Sun X., Salih E., Oppenheim F.G. J. Biol. Chem. 283:19957-19966(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING, MASS SPECTROMETRY.
[11]	"Finding new posttranslational modifications in salivary proline-rich proteins." Vitorino R., Alves R., Barros A., Caseiro A., Ferreira R., Lobo M.C., Bastos A., Duarte J., Carvalho D., Santos L.L., Amado F.L. Proteomics 10:3732-3742(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-87, PYROGLUTAMATE FORMATION, VARIANTS ALLELE L AND M, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

SHMPD

The Singapore human mutation and polymorphism database

Cross-references

Sequence databases
EMBL GenBank DDBJ	K03207 mRNA. Translation: AAA60188.1. X07882 Genomic DNA. Translation: CAA30729.1. Sequence problems. X07715 Genomic DNA. Translation: CAA30543.1. Sequence problems. AC010176 Genomic DNA. No translation available. BC130386 mRNA. Translation: AAI30387.1. S80916 Genomic DNA. Translation: AAB50687.2. X07704 Genomic DNA. Translation: CAA30542.1.
IPI	IPI00019482.
PIR	PIHUSD. S03176.
RefSeq	NP_001248328.1. NM_001261399.1. NP_002714.2. NM_002723.4.
UniGene	Hs.528651.
3D structure databases
DisProt	DP00119.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000279575.
Polymorphism databases
DMDM	158517854.
Proteomic databases
PaxDb	P10163.
PRIDE	P10163.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5545.
KEGG	hsa:5545.
Organism-specific databases
CTD	5545.
GeneCards	GC12M011460.
H-InvDB	HIX0079490.
HGNC	HGNC:9340. PRB4.
MIM	180990. gene.
neXtProt	NX_P10163.
PharmGKB	PA33702.
GenAtlas	Search...
Gene expression databases
CleanEx	HS_PRB4.
Genevestigator	P10163.
GermOnline	ENSG00000121335. Homo sapiens.
Family and domain databases
InterPro	IPR026086. Pro-rich. [Graphical view]
PANTHER	PTHR23203. PTHR23203. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	5545.
NextBio	21484.
SOURCE	Search...

Entry information

Entry name

PRB4_HUMAN

Accession

Primary (citable) accession number: P10163
Secondary accession number(s): A1L439 , O00600, P02813, P10161, P10162, P81489

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	September 11, 2007
Last modified:	April 3, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.