P10154 (TNFB_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lymphotoxin-alpha
Short name=LT-alpha

Alternative name(s):
TNF-beta
Tumor necrosis factor ligand superfamily member 1

Gene names

Name:	LTA
Synonyms:	TNFB, TNFSF1

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Subunit structure	Homotrimer, and heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits By similarity.
Subcellular location	Secreted By similarity. Membrane By similarity. Note: The homotrimer is secreted By similarity. The heterotrimer is membrane-associated By similarity.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Membrane Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to Gram-positive bacterium Inferred from electronic annotation. Source: Compara humoral immune response Inferred from electronic annotation. Source: Compara lymph node development Inferred from electronic annotation. Source: Compara negative regulation of growth of symbiont in host Inferred from electronic annotation. Source: Compara positive regulation of chronic inflammatory response to antigenic stimulus Inferred from electronic annotation. Source: Compara positive regulation of humoral immune response mediated by circulating immunoglobulin Inferred from electronic annotation. Source: Compara positive regulation of interferon-gamma production Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 197	171	Lymphotoxin-alpha		PRO_0000034470
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P10154 [UniParc]. Last modified August 1, 1991. Version 2. Checksum: 0CF18CC90B5E2345	FASTA	197	21,126
	10 20 30 40 50 60 MTPPGRLYLP LLLGLLLAPP PPGAQGLPGA EFPPSAARNA QQRLQKHFGH STLKPAAHLV 70 80 90 100 110 120 GDPSAQDSLR WRANTDRAFL RHGFSLSNNS LLVPSSGLYF VYSQVVFSGE GCSPKAVPTP 130 140 150 160 170 180 LYLAHEVQLF SSQYSFHVPL LSAQKSVCPG PQGPWVRSVY QGAVFLLTQG DQLSTHTDGI 190 AHLLLSPSSV FFGAFAL

References

[1]	"Structural analysis of the rabbit TNF locus, containing the genes encoding TNF-beta (lymphotoxin) and TNF-alpha (tumor necrosis factor)." Shakhov A.N., Kuprash D.V., Azizov M.M., Jongeneel C.V., Nedospasov S.A. Gene 95:215-221(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and structural analysis of genes coding for tumor necrosis factor and lymphotoxin in rabbits." Shakhov A.N., Kuprash D.V., Turetskaya R.L., Azizov M.M., Andreyeva A.V., Nedospasov S.A. Mol. Biol. (Mosk.) 23:1743-1750(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55745 Genomic DNA. Translation: CAA39275.1. Sequence problems. M60340 Genomic DNA. Translation: AAA31483.1. M60341 Genomic DNA. Translation: AAA31485.1.
PIR	JH0309.
RefSeq	NP_001164590.1. NM_001171119.1.
UniGene	Ocu.7553.
3D structure databases
ProteinModelPortal	P10154.
SMR	P10154. Positions 54-197.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000005782.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSOCUT00000006691; ENSOCUP00000005782; ENSOCUG00000006694.
GeneID	100328931.
Organism-specific databases
CTD	4049.
Phylogenomic databases
eggNOG	NOG41746.
GeneTree	ENSGT00530000062992.
HOGENOM	HOG000048729.
HOVERGEN	HBG012516.
OMA	QGPWVRS.
Family and domain databases
Gene3D	2.60.120.40. 1 hit.
InterPro	IPR006052. TNF. IPR006053. TNF_a/b/c. IPR002960. TNF_beta. IPR021184. TNF_CS. IPR008983. Tumour_necrosis_fac-like_dom. [Graphical view]
Pfam	PF00229. TNF. 1 hit. [Graphical view]
PRINTS	PR01234. TNECROSISFCT. PR01236. TNFBETA.
SMART	SM00207. TNF. 1 hit. [Graphical view]
SUPFAM	SSF49842. TNF_like. 1 hit.
PROSITE	PS00251. TNF_1. 1 hit. PS50049. TNF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TNFB_RABIT

Accession

Primary (citable) accession number: P10154

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program