P10151 (LEUO_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator LeuO

Gene names

Name:	leuO
Ordered Locus Names:	b0076, JW0075

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	A global transcription factor. Activates transcription of the 9 following operons; yjjQ-bglJ, yjjP, acrEF, ybdO, yjcRQP, casABCDE12, rhsD-ybbC, fepE and gltF, in most cases it probably interferes with silencing by H-NS and activates transcription. Represses transcription of the 3 following operons; uxaCA, sdaCB and yjiY. H-NS repression of the bgl operon, leading to the ability to metabolize some beta-glucosides. It also directly activates the bgl operon. Activation is H-NS and BglJ-RcsB independent. Ref.9 Ref.11
Induction	Gene expression is repressed by H-NS, activated by itself. More highly expressed in minimal than rich medium, poorly expressed in exoponential growth, levels increase in stationary phase (at protein level). Ref.9
Disruption phenotype	Has no effect on leucine biosynthesis nor on bgl operon silencing. Ref.7
Sequence similarities	Contains 1 HTH lysR-type DNA-binding domain.
Sequence caution	The sequence AAA83880.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA38853.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.7. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	HTH-type transcriptional regulator LeuO		PRO_0000105660
Regions
	Domain	22 – 79	58	HTH lysR-type
	DNA binding	39 – 58	20	H-T-H motif By similarity
Experimental info
	Sequence conflict	291 – 314	24	Missing in AAA85299. Ref.1
	Sequence conflict	291 – 314	24	Missing in CAA38853. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P10151 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 25605FA08C1073E3	FASTA	314	35,695
	10 20 30 40 50 60 MPEVQTDHPE TAELSKPQLR MVDLNLLTVF DAVMQEQNIT RAAHVLGMSQ PAVSNAVARL 70 80 90 100 110 120 KVMFNDELFV RYGRGIQPTA RAFQLFGSVR QALQLVQNEL PGSGFEPASS ERVFHLCVCS 130 140 150 160 170 180 PLDSILTSQI YNHIEQIAPN IHVMFKSSLN QNTEHQLRYQ ETEFVISYED FHRPEFTSVP 190 200 210 220 230 240 LFKDEMVLVA SKNHPTIKGP LLKHDVYNEQ HAAVSLDRFA SFSQPWYDTV DKQASIAYQG 250 260 270 280 290 300 MAMMSVLSVV SQTHLVAIAP RWLAEEFAES LELQVLPLPL KQNSRTCYLS WHEAAGRDKG 310 HQWMEEQLVS ICKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A large family of bacterial activator proteins." Henikoff S., Haughn G.W., Calvo J.M., Wallace J.C. Proc. Natl. Acad. Sci. U.S.A. 85:6602-6606(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 291-314. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"High A + T content conserved in DNA sequences upstream of leuABCD in Escherichia coli and Salmonella typhimurium." Haughn G.W., Wessler S.R., Gemmill R.M., Calvo J.M. J. Bacteriol. 166:1113-1117(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74. Strain: K12.
[6]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
[7]	"The leuO gene product has a latent ability to relieve bgl silencing in Escherichia coli." Ueguchi C., Ohta T., Seto C., Suzuki T., Mizuno T. J. Bacteriol. 180:190-193(1998) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE AS A TRANSCRIPTIONAL REGULATOR, DISRUPTION PHENOTYPE. Strain: K12 / CSH26.
[8]	"Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO." Stratmann T., Madhusudan S., Schnetz K. J. Bacteriol. 190:926-935(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING. Strain: CSH50.
[9]	"Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux." Shimada T., Yamamoto K., Ishihama A. J. Bacteriol. 191:4562-4571(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TRANSCRIPTIONAL ACTIVATOR, FUNCTION AS A TRANSCRIPTIONAL REPRESSOR, DNA-BINDING, INDUCTION. Strain: K12 / BW25113.
[10]	"BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS." Venkatesh G.R., Kembou Koungni F.C., Paukner A., Stratmann T., Blissenbach B., Schnetz K. J. Bacteriol. 192:6456-6464(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE AS A TRANSCRIPTIONAL REGULATOR, DNA-BINDING, INDEPENDENCE OF BGLJ-RCSB.
[11]	"H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO." Westra E.R., Pul U., Heidrich N., Jore M.M., Lundgren M., Stratmann T., Wurm R., Raine A., Mescher M., Van Heereveld L., Mastop M., Wagner E.G., Schnetz K., Van Der Oost J., Wagner R., Brouns S.J. Mol. Microbiol. 77:1380-1393(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ANTAGONIZES H-NS, ROLE IN CRISPR EXPRESSION. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21150 Genomic DNA. Translation: AAA85299.1. X55034 Genomic DNA. Translation: CAA38853.1. Different initiation. U00096 Genomic DNA. Translation: AAC73187.2. AP009048 Genomic DNA. Translation: BAB96645.2. M12891 Genomic DNA. Translation: AAA83880.1. Different initiation.
PIR	D64729.
RefSeq	NP_414618.4. NC_000913.2. YP_488382.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P10151.
SMR	P10151. Positions 28-308.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10151. 2 interactions.
STRING	511145.b0076.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73187; AAC73187; b0076. BAB96645; BAB96645; BAB96645.
GeneID	12930490. 949034.
KEGG	ecj:Y75_p0076. eco:b0076.
PATRIC	32115255. VBIEscCol129921_0079.
Organism-specific databases
EchoBASE	EB0526.
EcoGene	EG10531. leuO.
Phylogenomic databases
eggNOG	COG0583.
HOGENOM	HOG000116777.
KO	K05798.
OMA	QWMEELL.
ProtClustDB	PRK09508.
Enzyme and pathway databases
BioCyc	EcoCyc:PD00519. ECOL316407:JW0075-MONOMER.
Gene expression databases
Genevestigator	P10151.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005119. LysR_subst-bd. IPR000847. Tscrpt_reg_HTH_LysR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view]
PRINTS	PR00039. HTHLYSR.
PROSITE	PS50931. HTH_LYSR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEUO_ECOLI

Accession

Primary (citable) accession number: P10151
Secondary accession number(s): P75640

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program