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Protein

HTH-type transcriptional regulator LeuO

Gene

leuO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A global transcription factor. Activates transcription of the 9 following operons; yjjQ-bglJ, yjjP, acrEF, ybdO, yjcRQP, casABCDE12, rhsD-ybbC, fepE and gltF, in most cases it probably interferes with silencing by H-NS and activates transcription. Represses transcription of the 3 following operons; uxaCA, sdaCB and yjiY. H-NS repression of the bgl operon, leading to the ability to metabolize some beta-glucosides. It also directly activates the bgl operon. Activation is H-NS and BglJ-RcsB independent.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi39 – 58H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00519.
ECOL316407:JW0075-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator LeuO
Gene namesi
Name:leuO
Ordered Locus Names:b0076, JW0075
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10531. leuO.

Pathology & Biotechi

Disruption phenotypei

Has no effect on leucine biosynthesis nor on bgl operon silencing.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001056601 – 314HTH-type transcriptional regulator LeuOAdd BLAST314

Proteomic databases

PaxDbiP10151.
PRIDEiP10151.

Expressioni

Inductioni

Gene expression is repressed by H-NS, activated by itself. More highly expressed in minimal than rich medium, poorly expressed in exoponential growth, levels increase in stationary phase (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259484. 4 interactors.
IntActiP10151. 5 interactors.
STRINGi511145.b0076.

Structurei

3D structure databases

ProteinModelPortaliP10151.
SMRiP10151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 79HTH lysR-typePROSITE-ProRule annotationAdd BLAST58

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41064T2. Bacteria.
ENOG4110BBF. LUCA.
HOGENOMiHOG000116777.
InParanoidiP10151.
KOiK05798.
OMAiAPNIHLV.
PhylomeDBiP10151.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEVQTDHPE TAELSKPQLR MVDLNLLTVF DAVMQEQNIT RAAHVLGMSQ
60 70 80 90 100
PAVSNAVARL KVMFNDELFV RYGRGIQPTA RAFQLFGSVR QALQLVQNEL
110 120 130 140 150
PGSGFEPASS ERVFHLCVCS PLDSILTSQI YNHIEQIAPN IHVMFKSSLN
160 170 180 190 200
QNTEHQLRYQ ETEFVISYED FHRPEFTSVP LFKDEMVLVA SKNHPTIKGP
210 220 230 240 250
LLKHDVYNEQ HAAVSLDRFA SFSQPWYDTV DKQASIAYQG MAMMSVLSVV
260 270 280 290 300
SQTHLVAIAP RWLAEEFAES LELQVLPLPL KQNSRTCYLS WHEAAGRDKG
310
HQWMEEQLVS ICKR
Length:314
Mass (Da):35,695
Last modified:November 1, 1997 - v3
Checksum:i25605FA08C1073E3
GO

Sequence cautioni

The sequence AAA83880 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA38853 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti291 – 314Missing in AAA85299 (PubMed:3413113).CuratedAdd BLAST24
Sequence conflicti291 – 314Missing in CAA38853 (PubMed:1630901).CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21150 Genomic DNA. Translation: AAA85299.1.
X55034 Genomic DNA. Translation: CAA38853.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73187.2.
AP009048 Genomic DNA. Translation: BAB96645.2.
M12891 Genomic DNA. Translation: AAA83880.1. Different initiation.
PIRiD64729.
RefSeqiNP_414618.4. NC_000913.3.
WP_001115472.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73187; AAC73187; b0076.
BAB96645; BAB96645; BAB96645.
GeneIDi949034.
KEGGiecj:JW0075.
eco:b0076.
PATRICi32115255. VBIEscCol129921_0079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21150 Genomic DNA. Translation: AAA85299.1.
X55034 Genomic DNA. Translation: CAA38853.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73187.2.
AP009048 Genomic DNA. Translation: BAB96645.2.
M12891 Genomic DNA. Translation: AAA83880.1. Different initiation.
PIRiD64729.
RefSeqiNP_414618.4. NC_000913.3.
WP_001115472.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP10151.
SMRiP10151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259484. 4 interactors.
IntActiP10151. 5 interactors.
STRINGi511145.b0076.

Proteomic databases

PaxDbiP10151.
PRIDEiP10151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73187; AAC73187; b0076.
BAB96645; BAB96645; BAB96645.
GeneIDi949034.
KEGGiecj:JW0075.
eco:b0076.
PATRICi32115255. VBIEscCol129921_0079.

Organism-specific databases

EchoBASEiEB0526.
EcoGeneiEG10531. leuO.

Phylogenomic databases

eggNOGiENOG41064T2. Bacteria.
ENOG4110BBF. LUCA.
HOGENOMiHOG000116777.
InParanoidiP10151.
KOiK05798.
OMAiAPNIHLV.
PhylomeDBiP10151.

Enzyme and pathway databases

BioCyciEcoCyc:PD00519.
ECOL316407:JW0075-MONOMER.

Miscellaneous databases

PROiP10151.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEUO_ECOLI
AccessioniPrimary (citable) accession number: P10151
Secondary accession number(s): P75640
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.