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P10149

- STXA_BPH30

UniProt

P10149 - STXA_BPH30

Protein

Shiga-like toxin 1 subunit A

Gene

stxA

Organism
Bacteriophage H30
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    • Comment

    Functioni

    The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

    Catalytic activityi

    Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei189 – 1891By similarity
    Sitei273 – 2742Cleavage; by furinBy similarity

    GO - Molecular functioni

    1. rRNA N-glycosylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. negative regulation of translation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein synthesis inhibitor, Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shiga-like toxin 1 subunit A (EC:3.2.2.22)
    Short name:
    SLT-1 A subunit
    Short name:
    SLT-1a
    Short name:
    SLT-Ia
    Alternative name(s):
    Verocytotoxin 1 subunit A
    Verotoxin 1 subunit A
    rRNA N-glycosidase 1
    Gene namesi
    Name:stxA
    OrganismiBacteriophage H30
    Taxonomic identifieri12371 [NCBI]
    Taxonomic lineageiVirusesunclassified phages
    Virus hostiEscherichia coli [TaxID: 562]

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Add
    BLAST
    Chaini23 – 315293Shiga-like toxin 1 subunit APRO_0000030790Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi264 ↔ 283By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Shiga-like toxin contains a single subunit A and five copies of subunit B.

    Protein-protein interaction databases

    DIPiDIP-6144N.

    Structurei

    3D structure databases

    ProteinModelPortaliP10149.
    SMRiP10149. Positions 23-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni23 – 273251A1By similarityAdd
    BLAST
    Regioni274 – 31542A2By similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.420.10. 1 hit.
    4.10.470.10. 1 hit.
    InterProiIPR001574. Ribosome_inactivat_prot.
    IPR017988. Ribosome_inactivat_prot_CS.
    IPR016138. Ribosome_inactivat_prot_sub1.
    IPR016139. Ribosome_inactivat_prot_sub2.
    IPR016331. Shiga-like_toxin_subunit_A.
    [Graphical view]
    PfamiPF00161. RIP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001924. Shigella_toxin_subunit_A. 1 hit.
    SUPFAMiSSF56371. SSF56371. 1 hit.
    PROSITEiPS00275. SHIGA_RICIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10149-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIIIFRVLT FFFVIFSVNV VAKEFTLDFS TAKTYVDSLN VIRSAIGTPL    50
    QTISSGGTSL LMIDSGTGDN LFAVDVRGID PEEGRFNNLR LIVERNNLYV 100
    TGFVNRTNNV FYRFADFSHV TFPGTTAVTL SGDSSYTTLQ RVAGISRTGM 150
    QINRHSLTTS YLDLMSHSGT SLTQSVARAM LRFVTVTAEA LRFRQIQRGF 200
    RTTLDDLSGR SYVMTAEDVD LTLNWGRLSS VLPDYHGQDS VRVGRISFGS 250
    INAILGSVAL ILNCHHHASR VARMASDEFP SMCPADGRVR GITHNKILWD 300
    SSTLGAILMR RTISS 315
    Length:315
    Mass (Da):34,814
    Last modified:July 1, 1989 - v1
    Checksum:i8A423DF7ABF58F30
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23980 Genomic DNA. Translation: AAA72732.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23980 Genomic DNA. Translation: AAA72732.1 .

    3D structure databases

    ProteinModelPortali P10149.
    SMRi P10149. Positions 23-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6144N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.420.10. 1 hit.
    4.10.470.10. 1 hit.
    InterProi IPR001574. Ribosome_inactivat_prot.
    IPR017988. Ribosome_inactivat_prot_CS.
    IPR016138. Ribosome_inactivat_prot_sub1.
    IPR016139. Ribosome_inactivat_prot_sub2.
    IPR016331. Shiga-like_toxin_subunit_A.
    [Graphical view ]
    Pfami PF00161. RIP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001924. Shigella_toxin_subunit_A. 1 hit.
    SUPFAMi SSF56371. SSF56371. 1 hit.
    PROSITEi PS00275. SHIGA_RICIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The primary structure of the operons coding for Shigella dysenteriae toxin and temperature phage H30 shiga-like toxin."
      Kozlov Y.V., Kabishev A.A., Lukyanov E.V., Bayev A.A.
      Gene 67:213-221(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiSTXA_BPH30
    AccessioniPrimary (citable) accession number: P10149
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3