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Protein

Shiga-like toxin 1 subunit A

Gene

stxA

Organism
Bacteriophage H30
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891By similarity
Sitei273 – 2742Cleavage; by furinBy similarity

GO - Molecular functioni

  1. rRNA N-glycosylase activity Source: UniProtKB-EC

GO - Biological processi

  1. negative regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein synthesis inhibitor, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga-like toxin 1 subunit A (EC:3.2.2.22)
Short name:
SLT-1 A subunit
Short name:
SLT-1a
Short name:
SLT-Ia
Alternative name(s):
Verocytotoxin 1 subunit A
Verotoxin 1 subunit A
rRNA N-glycosidase 1
Gene namesi
Name:stxA
OrganismiBacteriophage H30
Taxonomic identifieri12371 [NCBI]
Taxonomic lineageiVirusesunclassified phages
Virus hostiEscherichia coli [TaxID: 562]

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Chaini23 – 315293Shiga-like toxin 1 subunit APRO_0000030790Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi264 ↔ 283By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Shiga-like toxin contains a single subunit A and five copies of subunit B.

Protein-protein interaction databases

DIPiDIP-6144N.

Structurei

3D structure databases

ProteinModelPortaliP10149.
SMRiP10149. Positions 23-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 273251A1By similarityAdd
BLAST
Regioni274 – 31542A2By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PIRSFiPIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10149-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIIIFRVLT FFFVIFSVNV VAKEFTLDFS TAKTYVDSLN VIRSAIGTPL
60 70 80 90 100
QTISSGGTSL LMIDSGTGDN LFAVDVRGID PEEGRFNNLR LIVERNNLYV
110 120 130 140 150
TGFVNRTNNV FYRFADFSHV TFPGTTAVTL SGDSSYTTLQ RVAGISRTGM
160 170 180 190 200
QINRHSLTTS YLDLMSHSGT SLTQSVARAM LRFVTVTAEA LRFRQIQRGF
210 220 230 240 250
RTTLDDLSGR SYVMTAEDVD LTLNWGRLSS VLPDYHGQDS VRVGRISFGS
260 270 280 290 300
INAILGSVAL ILNCHHHASR VARMASDEFP SMCPADGRVR GITHNKILWD
310
SSTLGAILMR RTISS
Length:315
Mass (Da):34,814
Last modified:July 1, 1989 - v1
Checksum:i8A423DF7ABF58F30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23980 Genomic DNA. Translation: AAA72732.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23980 Genomic DNA. Translation: AAA72732.1.

3D structure databases

ProteinModelPortaliP10149.
SMRiP10149. Positions 23-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6144N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PIRSFiPIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The primary structure of the operons coding for Shigella dysenteriae toxin and temperature phage H30 shiga-like toxin."
    Kozlov Y.V., Kabishev A.A., Lukyanov E.V., Bayev A.A.
    Gene 67:213-221(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiSTXA_BPH30
AccessioniPrimary (citable) accession number: P10149
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.