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Protein

C-C motif chemokine 2

Gene

Ccl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chemotactic factor that attracts monocytes, but not neutrophils.

GO - Molecular functioni

  1. CCR2 chemokine receptor binding Source: BHF-UCL
  2. chemokine activity Source: MGI
  3. G-protein coupled receptor binding Source: MGI
  4. heparin binding Source: Ensembl

GO - Biological processi

  1. aging Source: Ensembl
  2. angiogenesis Source: BHF-UCL
  3. astrocyte cell migration Source: BHF-UCL
  4. cellular calcium ion homeostasis Source: Ensembl
  5. cellular response to ATP Source: Ensembl
  6. cellular response to dexamethasone stimulus Source: Ensembl
  7. cellular response to drug Source: Ensembl
  8. cellular response to fibroblast growth factor stimulus Source: Ensembl
  9. cellular response to high density lipoprotein particle stimulus Source: Ensembl
  10. cellular response to insulin stimulus Source: Ensembl
  11. cellular response to interferon-gamma Source: BHF-UCL
  12. cellular response to interleukin-1 Source: BHF-UCL
  13. cellular response to interleukin-6 Source: Ensembl
  14. cellular response to lipopolysaccharide Source: BHF-UCL
  15. cellular response to macrophage colony-stimulating factor stimulus Source: Ensembl
  16. cellular response to platelet-derived growth factor stimulus Source: Ensembl
  17. cellular response to retinoic acid Source: Ensembl
  18. cellular response to tumor necrosis factor Source: BHF-UCL
  19. chemokine-mediated signaling pathway Source: Ensembl
  20. cytoskeleton organization Source: MGI
  21. eosinophil chemotaxis Source: MGI
  22. helper T cell extravasation Source: BHF-UCL
  23. leukocyte migration involved in inflammatory response Source: Ensembl
  24. lipopolysaccharide-mediated signaling pathway Source: Ensembl
  25. lymphocyte chemotaxis Source: Ensembl
  26. macrophage chemotaxis Source: BHF-UCL
  27. MAPK cascade Source: Ensembl
  28. maternal process involved in female pregnancy Source: Ensembl
  29. maternal process involved in parturition Source: Ensembl
  30. monocyte chemotaxis Source: Ensembl
  31. monocyte homeostasis Source: BHF-UCL
  32. negative regulation of angiogenesis Source: BHF-UCL
  33. negative regulation of glial cell apoptotic process Source: Ensembl
  34. negative regulation of natural killer cell chemotaxis Source: Ensembl
  35. negative regulation of neuron apoptotic process Source: Ensembl
  36. neutrophil chemotaxis Source: Ensembl
  37. organ regeneration Source: Ensembl
  38. positive regulation of apoptotic cell clearance Source: BHF-UCL
  39. positive regulation of calcium ion import Source: Ensembl
  40. positive regulation of cellular extravasation Source: Ensembl
  41. positive regulation of collagen biosynthetic process Source: Ensembl
  42. positive regulation of endothelial cell proliferation Source: Ensembl
  43. positive regulation of immune complex clearance by monocytes and macrophages Source: BHF-UCL
  44. positive regulation of leukocyte mediated cytotoxicity Source: Ensembl
  45. positive regulation of macrophage chemotaxis Source: BHF-UCL
  46. positive regulation of monocyte chemotaxis Source: Ensembl
  47. positive regulation of nitric-oxide synthase biosynthetic process Source: BHF-UCL
  48. positive regulation of synaptic transmission Source: Ensembl
  49. positive regulation of T cell activation Source: BHF-UCL
  50. positive regulation of tumor necrosis factor production Source: Ensembl
  51. protein kinase B signaling Source: Ensembl
  52. regulation of cell shape Source: MGI
  53. regulation of vascular endothelial growth factor production Source: BHF-UCL
  54. response to activity Source: Ensembl
  55. response to amino acid Source: Ensembl
  56. response to antibiotic Source: Ensembl
  57. response to ethanol Source: Ensembl
  58. response to gamma radiation Source: Ensembl
  59. response to heat Source: Ensembl
  60. response to hypoxia Source: Ensembl
  61. response to mechanical stimulus Source: Ensembl
  62. response to progesterone Source: Ensembl
  63. response to vitamin B3 Source: Ensembl
  64. response to wounding Source: MGI
  65. transforming growth factor beta receptor signaling pathway Source: Ensembl
  66. vascular endothelial growth factor receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 2
Alternative name(s):
Monocyte chemoattractant protein 1
Monocyte chemotactic protein 1
Short name:
MCP-1
Platelet-derived growth factor-inducible protein JE
Small-inducible cytokine A2
Gene namesi
Name:Ccl2
Synonyms:Je, Mcp1, Scya2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98259. Ccl2.

Subcellular locationi

GO - Cellular componenti

  1. axon terminus Source: Ensembl
  2. C-fiber Source: Ensembl
  3. dendrite Source: Ensembl
  4. endocytic vesicle Source: Ensembl
  5. extracellular space Source: MGI
  6. perikaryon Source: Ensembl
  7. perinuclear region of cytoplasm Source: Ensembl
  8. rough endoplasmic reticulum Source: Ensembl
  9. synapse Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 148125C-C motif chemokine 2PRO_0000005149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Pyrrolidone carboxylic acidBy similarity
Disulfide bondi34 ↔ 59By similarity
Disulfide bondi35 ↔ 75By similarity
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PRIDEiP10148.

PTM databases

PhosphoSiteiP10148.

Expressioni

Inductioni

By platelet-derived growth factor. Up-regulated upon hypertonic conditions.1 Publication

Gene expression databases

BgeeiP10148.
CleanExiMM_CCL2.
ExpressionAtlasiP10148. baseline and differential.
GenevestigatoriP10148.

Interactioni

Subunit structurei

Monomer or homodimer; in equilibrium.By similarity

Protein-protein interaction databases

BioGridi203121. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP10148.
SMRiP10148. Positions 24-92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46819.
HOGENOMiHOG000036686.
HOVERGENiHBG017871.
InParanoidiP10148.
KOiK14624.
OMAiNAPLTCC.
OrthoDBiEOG7673DP.
PhylomeDBiP10148.
TreeFamiTF339372.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVPVMLLGL LFTVAGWSIH VLAQPDAVNA PLTCCYSFTS KMIPMSRLES
60 70 80 90 100
YKRITSSRCP KEAVVFVTKL KREVCADPKK EWVQTYIKNL DRNQMRSEPT
110 120 130 140
TLFKTASALR SSAPLNVKLT RKSEANASTT FSTTTSSTSV GVTSVTVN
Length:148
Mass (Da):16,326
Last modified:July 1, 1989 - v1
Checksum:i29A5505FC6D94837
GO

Polymorphismi

The polymorphisms in strain SJL/J may be associated with severity of clinical symptoms of experimental allergic encephalomyelitis, an animal model of multiple sclerosis, and susceptibility to the monophasic remitting/nonrelapsing form of the disease.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501S → G in strain: SJL/J. 1 Publication
Natural varianti92 – 921R → Q in strain: SJL/J. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04467 Genomic DNA. Translation: AAA37685.1.
M19681 Genomic DNA. Translation: AAA37684.1.
AF065929 mRNA. Translation: AAF15379.1.
AF065930 mRNA. Translation: AAF15380.1.
AF065931 mRNA. Translation: AAF15381.1.
AF065932 mRNA. Translation: AAF15382.1.
AF065933 mRNA. Translation: AAF15383.1.
CCDSiCCDS25139.1.
PIRiA30209.
S16226.
RefSeqiNP_035463.1. NM_011333.3.
UniGeneiMm.290320.

Genome annotation databases

EnsembliENSMUST00000000193; ENSMUSP00000000193; ENSMUSG00000035385.
GeneIDi20296.
KEGGimmu:20296.
UCSCiuc007kmp.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04467 Genomic DNA. Translation: AAA37685.1.
M19681 Genomic DNA. Translation: AAA37684.1.
AF065929 mRNA. Translation: AAF15379.1.
AF065930 mRNA. Translation: AAF15380.1.
AF065931 mRNA. Translation: AAF15381.1.
AF065932 mRNA. Translation: AAF15382.1.
AF065933 mRNA. Translation: AAF15383.1.
CCDSiCCDS25139.1.
PIRiA30209.
S16226.
RefSeqiNP_035463.1. NM_011333.3.
UniGeneiMm.290320.

3D structure databases

ProteinModelPortaliP10148.
SMRiP10148. Positions 24-92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203121. 2 interactions.

PTM databases

PhosphoSiteiP10148.

Proteomic databases

PRIDEiP10148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000193; ENSMUSP00000000193; ENSMUSG00000035385.
GeneIDi20296.
KEGGimmu:20296.
UCSCiuc007kmp.1. mouse.

Organism-specific databases

CTDi6347.
MGIiMGI:98259. Ccl2.

Phylogenomic databases

eggNOGiNOG46819.
HOGENOMiHOG000036686.
HOVERGENiHBG017871.
InParanoidiP10148.
KOiK14624.
OMAiNAPLTCC.
OrthoDBiEOG7673DP.
PhylomeDBiP10148.
TreeFamiTF339372.

Miscellaneous databases

NextBioi298021.
PROiP10148.
SOURCEiSearch...

Gene expression databases

BgeeiP10148.
CleanExiMM_CCL2.
ExpressionAtlasiP10148. baseline and differential.
GenevestigatoriP10148.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Platelet-derived growth factor-inducible gene JE is a member of a family of small inducible genes related to platelet factor 4."
    Kawahara R.S., Deuel T.F.
    J. Biol. Chem. 264:679-682(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties."
    Rollins B.J., Morrison E.D., Stiles C.D.
    Proc. Natl. Acad. Sci. U.S.A. 85:3738-3742(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
    Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., Blankenhorn E.P.
    J. Immunol. 163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-50 AND GLN-92.
    Strain: B10.S/J, BALB/c, DBA/2J, NOD/LtJ and SJL/J.
    Tissue: Spleen.
  4. "Production and identification of natural monocyte chemotactic protein from virally infected murine fibroblasts. Relationship with the product of the mouse competence (JE) gene."
    van Damme J., Decock B., Bertini R., Conings R., Lenaerts J.-P., Put W., Opdenakker G., Mantovani A.
    Eur. J. Biochem. 199:223-229(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-42.
  5. "Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress."
    Ueno M., Shen W.J., Patel S., Greenberg A.S., Azhar S., Kraemer F.B.
    J. Lipid Res. 54:734-743(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OSMOTIC STRESS.

Entry informationi

Entry nameiCCL2_MOUSE
AccessioniPrimary (citable) accession number: P10148
Secondary accession number(s): Q9QYD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 4, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.