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P10148 (CCL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 2
Alternative name(s):
Monocyte chemoattractant protein 1
Monocyte chemotactic protein 1
Short name=MCP-1
Platelet-derived growth factor-inducible protein JE
Small-inducible cytokine A2
Gene names
Name:Ccl2
Synonyms:Je, Mcp1, Scya2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length148 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chemotactic factor that attracts monocytes, but not neutrophils.

Subunit structure

Monomer or homodimer; in equilibrium By similarity.

Subcellular location

Secreted.

Induction

By platelet-derived growth factor. Up-regulated upon hypertonic conditions. Ref.5

Polymorphism

The polymorphisms in strain SJL/J may be associated with severity of clinical symptoms of experimental allergic encephalomyelitis, an animal model of multiple sclerosis, and susceptibility to the monophasic remitting/nonrelapsing form of the disease.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
   Biological processChemotaxis
Inflammatory response
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
Glycoprotein
Pyrrolidone carboxylic acid
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from electronic annotation. Source: Ensembl

aging

Inferred from electronic annotation. Source: Ensembl

angiogenesis

Traceable author statement PubMed 18334747. Source: BHF-UCL

astrocyte cell migration

Inferred from sequence or structural similarity. Source: BHF-UCL

cellular calcium ion homeostasis

Inferred from electronic annotation. Source: Ensembl

cellular response to fibroblast growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to interferon-gamma

Inferred from direct assay PubMed 14662900. Source: BHF-UCL

cellular response to interleukin-1

Inferred from direct assay PubMed 14662900. Source: BHF-UCL

cellular response to lipopolysaccharide

Inferred from direct assay PubMed 14662900. Source: BHF-UCL

cellular response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

cellular response to tumor necrosis factor

Inferred from direct assay PubMed 14662900. Source: BHF-UCL

chemokine-mediated signaling pathway

Inferred from electronic annotation. Source: Ensembl

cytoskeleton organization

Inferred from electronic annotation. Source: Ensembl

helper T cell extravasation

Inferred from direct assay PubMed 18586982. Source: BHF-UCL

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

lipopolysaccharide-mediated signaling pathway

Inferred from electronic annotation. Source: Ensembl

lymphocyte chemotaxis

Inferred from electronic annotation. Source: Ensembl

macrophage chemotaxis

Inferred from sequence or structural similarity. Source: BHF-UCL

maternal process involved in parturition

Inferred from electronic annotation. Source: Ensembl

monocyte chemotaxis

Inferred from electronic annotation. Source: Ensembl

monocyte homeostasis

Traceable author statement PubMed 18334747. Source: BHF-UCL

negative regulation of angiogenesis

Inferred from mutant phenotype PubMed 14566334. Source: BHF-UCL

negative regulation of glial cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of natural killer cell chemotaxis

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

neutrophil chemotaxis

Inferred from electronic annotation. Source: Ensembl

organ regeneration

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell activation

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

positive regulation of apoptotic cell clearance

Inferred from direct assay PubMed 20691665. Source: BHF-UCL

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of immune complex clearance by monocytes and macrophages

Inferred from mutant phenotype PubMed 14566334. Source: BHF-UCL

positive regulation of macrophage chemotaxis

Inferred from direct assay PubMed 14566334. Source: BHF-UCL

positive regulation of nitric-oxide synthase biosynthetic process

Inferred from mutant phenotype PubMed 19164781. Source: BHF-UCL

positive regulation of synaptic transmission

Inferred from electronic annotation. Source: Ensembl

protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

regulation of cell shape

Inferred from electronic annotation. Source: Ensembl

regulation of vascular endothelial growth factor production

Inferred from mutant phenotype PubMed 14566334. Source: BHF-UCL

response to activity

Inferred from electronic annotation. Source: Ensembl

response to amino acid

Inferred from electronic annotation. Source: Ensembl

response to antibiotic

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to gamma radiation

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

response to heat

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

response to progesterone

Inferred from electronic annotation. Source: Ensembl

response to vitamin B3

Inferred from electronic annotation. Source: Ensembl

response to wounding

Inferred from direct assay PubMed 19404405. Source: MGI

transforming growth factor beta receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

vascular endothelial growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from direct assay PubMed 19139201. Source: MGI

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionCCR2 chemokine receptor binding

Inferred from physical interaction PubMed 8662823. Source: BHF-UCL

G-protein coupled receptor binding

Inferred from direct assay PubMed 8631787PubMed 9366570. Source: MGI

heparin binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 148125C-C motif chemokine 2
PRO_0000005149

Amino acid modifications

Modified residue241Pyrrolidone carboxylic acid By similarity
Glycosylation1261N-linked (GlcNAc...) Potential
Disulfide bond34 ↔ 59 By similarity
Disulfide bond35 ↔ 75 By similarity

Natural variations

Natural variant501S → G in strain: SJL/J. Ref.3
Natural variant921R → Q in strain: SJL/J. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P10148 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 29A5505FC6D94837

FASTA14816,326
        10         20         30         40         50         60 
MQVPVMLLGL LFTVAGWSIH VLAQPDAVNA PLTCCYSFTS KMIPMSRLES YKRITSSRCP 

        70         80         90        100        110        120 
KEAVVFVTKL KREVCADPKK EWVQTYIKNL DRNQMRSEPT TLFKTASALR SSAPLNVKLT 

       130        140 
RKSEANASTT FSTTTSSTSV GVTSVTVN 

« Hide

References

[1]"Platelet-derived growth factor-inducible gene JE is a member of a family of small inducible genes related to platelet factor 4."
Kawahara R.S., Deuel T.F.
J. Biol. Chem. 264:679-682(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties."
Rollins B.J., Morrison E.D., Stiles C.D.
Proc. Natl. Acad. Sci. U.S.A. 85:3738-3742(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., Blankenhorn E.P.
J. Immunol. 163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-50 AND GLN-92.
Strain: B10.S/J, BALB/c, DBA/2J, NOD/LtJ and SJL/J.
Tissue: Spleen.
[4]"Production and identification of natural monocyte chemotactic protein from virally infected murine fibroblasts. Relationship with the product of the mouse competence (JE) gene."
van Damme J., Decock B., Bertini R., Conings R., Lenaerts J.-P., Put W., Opdenakker G., Mantovani A.
Eur. J. Biochem. 199:223-229(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-42.
[5]"Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress."
Ueno M., Shen W.J., Patel S., Greenberg A.S., Azhar S., Kraemer F.B.
J. Lipid Res. 54:734-743(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY OSMOTIC STRESS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04467 Genomic DNA. Translation: AAA37685.1.
M19681 Genomic DNA. Translation: AAA37684.1.
AF065929 mRNA. Translation: AAF15379.1.
AF065930 mRNA. Translation: AAF15380.1.
AF065931 mRNA. Translation: AAF15381.1.
AF065932 mRNA. Translation: AAF15382.1.
AF065933 mRNA. Translation: AAF15383.1.
PIRA30209.
S16226.
RefSeqNP_035463.1. NM_011333.3.
UniGeneMm.290320.

3D structure databases

ProteinModelPortalP10148.
SMRP10148. Positions 24-92.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203121. 2 interactions.

PTM databases

PhosphoSiteP10148.

Proteomic databases

PRIDEP10148.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000193; ENSMUSP00000000193; ENSMUSG00000035385.
GeneID20296.
KEGGmmu:20296.
UCSCuc007kmp.1. mouse.

Organism-specific databases

CTD6347.
MGIMGI:98259. Ccl2.

Phylogenomic databases

eggNOGNOG46819.
HOGENOMHOG000036686.
HOVERGENHBG017871.
InParanoidP10148.
KOK14624.
OMANAPLTCC.
OrthoDBEOG7673DP.
PhylomeDBP10148.
TreeFamTF339372.

Gene expression databases

ArrayExpressP10148.
BgeeP10148.
CleanExMM_CCL2.
GenevestigatorP10148.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298021.
PROP10148.
SOURCESearch...

Entry information

Entry nameCCL2_MOUSE
AccessionPrimary (citable) accession number: P10148
Secondary accession number(s): Q9QYD7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot