SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P10147

- CCL3_HUMAN

UniProt

P10147 - CCL3_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
C-C motif chemokine 3
Gene
CCL3, G0S19-1, MIP1A, SCYA3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei40 – 401Involved in GAG binding
Sitei68 – 681Involved in GAG binding
Sitei70 – 701Involved in GAG binding

GO - Molecular functioni

  1. CCR1 chemokine receptor binding Source: UniProtKB
  2. CCR5 chemokine receptor binding Source: UniProtKB
  3. calcium-dependent protein kinase C activity Source: UniProtKB
  4. chemoattractant activity Source: UniProtKB
  5. chemokine activity Source: UniProtKB
  6. identical protein binding Source: IntAct
  7. kinase activity Source: UniProtKB
  8. phospholipase activator activity Source: UniProtKB
  9. protein binding Source: UniProtKB
  10. protein kinase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. MAPK cascade Source: UniProtKB
  2. T cell chemotaxis Source: UniProtKB
  3. astrocyte cell migration Source: UniProtKB
  4. behavior Source: UniProtKB
  5. calcium ion transport Source: UniProtKB
  6. calcium-mediated signaling Source: UniProtKB
  7. cell activation Source: UniProtKB
  8. cell-cell signaling Source: UniProtKB
  9. cellular calcium ion homeostasis Source: UniProtKB
  10. cellular response to interferon-gamma Source: UniProtKB
  11. cellular response to interleukin-1 Source: UniProtKB
  12. cellular response to organic cyclic compound Source: UniProtKB
  13. cellular response to tumor necrosis factor Source: UniProtKB
  14. chemotaxis Source: UniProtKB
  15. cytoskeleton organization Source: UniProtKB
  16. eosinophil chemotaxis Source: UniProtKB
  17. eosinophil degranulation Source: UniProtKB
  18. exocytosis Source: UniProtKB
  19. granulocyte chemotaxis Source: UniProtKB
  20. inflammatory response Source: UniProtKB
  21. lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  22. lymphocyte chemotaxis Source: UniProtKB
  23. macrophage chemotaxis Source: UniProtKB
  24. monocyte chemotaxis Source: UniProtKB
  25. negative regulation by host of viral transcription Source: UniProtKB
  26. negative regulation of bone mineralization Source: UniProtKB
  27. negative regulation of gene expression Source: UniProtKB
  28. negative regulation of osteoclast differentiation Source: UniProtKB
  29. neutrophil chemotaxis Source: UniProtKB
  30. osteoblast differentiation Source: UniProtKB
  31. positive chemotaxis Source: GOC
  32. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  33. positive regulation of calcium ion import Source: BHF-UCL
  34. positive regulation of calcium ion transport Source: UniProtKB
  35. positive regulation of calcium-mediated signaling Source: UniProtKB
  36. positive regulation of catalytic activity Source: GOC
  37. positive regulation of cell migration Source: UniProtKB
  38. positive regulation of gene expression Source: UniProtKB
  39. positive regulation of inflammatory response Source: UniProtKB
  40. positive regulation of interleukin-1 beta secretion Source: UniProtKB
  41. positive regulation of natural killer cell chemotaxis Source: UniProtKB
  42. positive regulation of neuron apoptotic process Source: UniProtKB
  43. positive regulation of protein kinase B signaling Source: UniProtKB
  44. positive regulation of tumor necrosis factor production Source: UniProtKB
  45. protein kinase B signaling Source: UniProtKB
  46. protein phosphorylation Source: GOC
  47. regulation of cell shape Source: UniProtKB
  48. regulation of sensory perception of pain Source: UniProtKB
  49. release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: UniProtKB
  50. response to cholesterol Source: UniProtKB
  51. response to toxic substance Source: UniProtKB
  52. signaling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Enzyme and pathway databases

ReactomeiREACT_15344. Chemokine receptors bind chemokines.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 3
Alternative name(s):
G0/G1 switch regulatory protein 19-1
Macrophage inflammatory protein 1-alpha
Short name:
MIP-1-alpha
PAT 464.1
SIS-beta
Small-inducible cytokine A3
Tonsillar lymphocyte LD78 alpha protein
Cleaved into the following chain:
Alternative name(s):
LD78-alpha(4-69)
Gene namesi
Name:CCL3
Synonyms:G0S19-1, MIP1A, SCYA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:10627. CCL3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. extracellular region Source: BHF-UCL
  4. extracellular space Source: UniProtKB
  5. intracellular Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi40 – 401R → A: Slightly reduces heparin binding. 1 Publication
Mutagenesisi49 – 491D → A: Reduces self-association; in BB-10010: Improved pharmaceutical properties. 2 Publications
Mutagenesisi68 – 681R → A: Strongly reduces heparin binding. 1 Publication
Mutagenesisi70 – 701R → A: Reduces heparin binding. 1 Publication
Mutagenesisi89 – 891E → A: Reduces self-association. 1 Publication

Organism-specific databases

PharmGKBiPA35559.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 Publication
Add
BLAST
Chaini24 – 9269C-C motif chemokine 3
PRO_0000005156Add
BLAST
Chaini27 – 9266MIP-1-alpha(4-69)
PRO_0000005157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 57 By similarity
Disulfide bondi34 ↔ 73 By similarity

Post-translational modificationi

N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP10147.
PRIDEiP10147.

Expressioni

Inductioni

By TPA or PHA (TPA = 12-O-tetradecanoyl phorbol-13 acetate (tumor promoter); PHA = phytohemagglutinin (T-cell mitogen)).

Gene expression databases

BgeeiP10147.
CleanExiHS_CCL3.
GenevestigatoriP10147.

Interactioni

Subunit structurei

Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-8459634,EBI-8459634
IDEP147353EBI-8459634,EBI-2556886

Protein-protein interaction databases

BioGridi112252. 4 interactions.
DIPiDIP-5837N.
IntActiP10147. 1 interaction.
MINTiMINT-103148.
STRINGi9606.ENSP00000225245.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 283
Beta strandi31 – 333
Helixi44 – 463
Beta strandi47 – 526
Beta strandi57 – 593
Beta strandi62 – 665
Beta strandi71 – 744
Helixi79 – 879

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B50NMR-A/B24-92[»]
1B53NMR-A/B24-92[»]
2X69X-ray2.65A/B/C/D/E23-92[»]
2X6GX-ray2.18A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R23-92[»]
3FPUX-ray1.76B24-92[»]
3H44X-ray3.00C/D23-92[»]
3KBXX-ray2.65A/B/C/D/E23-92[»]
3TN1X-ray2.60A/B/C/D/E25-91[»]
ProteinModelPortaliP10147.
SMRiP10147. Positions 26-91.

Miscellaneous databases

EvolutionaryTraceiP10147.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42742.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP10147.
KOiK05408.
OMAiNTPTACC.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP10147.
TreeFamiTF334888.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10147-1 [UniParc]FASTAAdd to Basket

« Hide

MQVSTAALAV LLCTMALCNQ FSASLAADTP TACCFSYTSR QIPQNFIADY   50
FETSSQCSKP GVIFLTKRSR QVCADPSEEW VQKYVSDLEL SA 92
Length:92
Mass (Da):10,085
Last modified:July 1, 1989 - v1
Checksum:i517865D5D6776CA8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti78 – 781E → D.
Corresponds to variant rs34171309 [ dbSNP | Ensembl ].
VAR_048701

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00044 mRNA. Translation: BAA00029.1.
X03754 mRNA. Translation: CAA27388.1.
X04018 Genomic DNA. Translation: CAA27643.1. Sequence problems.
M25315 mRNA. Translation: AAA57255.1.
M23452 mRNA. Translation: AAA36316.1.
M23178 Genomic DNA. Translation: AAA35858.1.
D90144 Genomic DNA. Translation: BAA14172.1.
BC071834 mRNA. Translation: AAH71834.1.
AF043339 mRNA. Translation: AAC03539.1.
CCDSiCCDS11307.1.
PIRiA35673. A30574.
RefSeqiNP_002974.1. NM_002983.2.
UniGeneiHs.514107.

Genome annotation databases

EnsembliENST00000225245; ENSP00000225245; ENSG00000006075.
GeneIDi6348.
KEGGihsa:6348.
UCSCiuc002hkv.3. human.

Polymorphism databases

DMDMi127078.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Macrophage inflammatory protein entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00044 mRNA. Translation: BAA00029.1 .
X03754 mRNA. Translation: CAA27388.1 .
X04018 Genomic DNA. Translation: CAA27643.1 . Sequence problems.
M25315 mRNA. Translation: AAA57255.1 .
M23452 mRNA. Translation: AAA36316.1 .
M23178 Genomic DNA. Translation: AAA35858.1 .
D90144 Genomic DNA. Translation: BAA14172.1 .
BC071834 mRNA. Translation: AAH71834.1 .
AF043339 mRNA. Translation: AAC03539.1 .
CCDSi CCDS11307.1.
PIRi A35673. A30574.
RefSeqi NP_002974.1. NM_002983.2.
UniGenei Hs.514107.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B50 NMR - A/B 24-92 [» ]
1B53 NMR - A/B 24-92 [» ]
2X69 X-ray 2.65 A/B/C/D/E 23-92 [» ]
2X6G X-ray 2.18 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R 23-92 [» ]
3FPU X-ray 1.76 B 24-92 [» ]
3H44 X-ray 3.00 C/D 23-92 [» ]
3KBX X-ray 2.65 A/B/C/D/E 23-92 [» ]
3TN1 X-ray 2.60 A/B/C/D/E 25-91 [» ]
ProteinModelPortali P10147.
SMRi P10147. Positions 26-91.
ModBasei Search...

Protein-protein interaction databases

BioGridi 112252. 4 interactions.
DIPi DIP-5837N.
IntActi P10147. 1 interaction.
MINTi MINT-103148.
STRINGi 9606.ENSP00000225245.

Polymorphism databases

DMDMi 127078.

Proteomic databases

PaxDbi P10147.
PRIDEi P10147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000225245 ; ENSP00000225245 ; ENSG00000006075 .
GeneIDi 6348.
KEGGi hsa:6348.
UCSCi uc002hkv.3. human.

Organism-specific databases

CTDi 6348.
GeneCardsi GC17M034415.
HGNCi HGNC:10627. CCL3.
MIMi 182283. gene.
neXtProti NX_P10147.
PharmGKBi PA35559.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG42742.
HOGENOMi HOG000036685.
HOVERGENi HBG017871.
InParanoidi P10147.
KOi K05408.
OMAi NTPTACC.
OrthoDBi EOG7CVQ1F.
PhylomeDBi P10147.
TreeFami TF334888.

Enzyme and pathway databases

Reactomei REACT_15344. Chemokine receptors bind chemokines.

Miscellaneous databases

EvolutionaryTracei P10147.
GeneWikii CCL3.
GenomeRNAii 6348.
NextBioi 24664.
PROi P10147.
SOURCEi Search...

Gene expression databases

Bgeei P10147.
CleanExi HS_CCL3.
Genevestigatori P10147.

Family and domain databases

InterProi IPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view ]
Pfami PF00048. IL8. 1 hit.
[Graphical view ]
SMARTi SM00199. SCY. 1 hit.
[Graphical view ]
SUPFAMi SSF54117. SSF54117. 1 hit.
PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A cDNA clone used to study mRNA inducible in human tonsillar lymphocytes by a tumor promoter."
    Obaru K., Fukuda M., Maeda S., Shimada K.
    J. Biochem. 99:885-894(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Lymphocyte.
  2. "Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors."
    Zipfel P.F., Balke J., Irving S.G., Kelly K., Siebenlist U.
    J. Immunol. 142:1582-1590(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: T-cell.
  3. "Three human homologs of a murine gene encoding an inhibitor of stem cell proliferation."
    Blum S., Forsdyke R.E., Forsdyke D.R.
    DNA Cell Biol. 9:589-602(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Lymphocyte.
  4. "Structures of human genes coding for cytokine LD78 and their expression."
    Nakao M., Nomiyama H., Shimada K.
    Mol. Cell. Biol. 10:3646-3658(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Lymphocyte.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Natural killer cell.
  6. Jang J.S., Kim B.E.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-92.
    Tissue: Leukocyte.
  7. "BB-10010: an active variant of human macrophage inflammatory protein-1 alpha with improved pharmaceutical properties."
    Hunter M.G., Bawden L., Brotherton D., Craig S., Cribbes S., Czaplewski L.G., Dexter T.M., Drummond A.H., Gearing A.H., Heyworth C.M., Lord B.I., Mccourt M., Varley P.G., Wood L.M., Edwards R.M., Lewis P.J.
    Blood 86:4400-4408(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-92, MUTAGENESIS OF ASP-49.
  8. "Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells."
    Cocchi F., DeVico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P.
    Science 270:1811-1815(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-40 AND 71-83, FUNCTION.
  9. "Identification of MIP-1 alpha/LD78 as a monocyte chemoattractant released by the HTLV-I-transformed cell line MT4."
    Bertini R., Luini W., Sozzani S., Bottazzi B., Ruggiero P., Boraschi D., Saggioro D., Chieco-Bianchi L., Proost P., van Damme J., Mantovani A.
    AIDS Res. Hum. Retroviruses 11:155-160(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-51, IDENTIFICATION OF LD78-ALPHA(4-69).
  10. "Identification of a glycosaminoglycan-binding site in chemokine macrophage inflammatory protein-1alpha."
    Koopmann W., Krangel M.S.
    J. Biol. Chem. 272:10103-10109(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GAG BINDING SITES ARG-40; ARG-68 AND ARG-70, MUTAGENESIS OF ARG-40; ARG-68 AND ARG-70.
  11. "Natural truncation of the chemokine MIP-1beta/CCL4 affects receptor specificity but not anti-HIV-1 activity."
    Guan E., Wang J., Roderiquez G., Norcross M.A.
    J. Biol. Chem. 277:32348-32352(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH MIP-1-BETA(3-69).
  12. Cited for: REVIEW.
  13. "Identification of amino acid residues critical for aggregation of human CC chemokines macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES. Characterization of active disaggregated chemokine variants."
    Czaplewski L.G., McKeating J., Craven C.J., Higgins L.D., Appay V., Brown A., Dudgeon T., Howard L.A., Meyers T., Owen J., Palan S.R., Tan P., Wilson G., Woods N.R., Heyworth C.M., Lord B.I., Brotherton D., Christison R.
    , Craig S., Cribbes S., Edwards R.M., Evans S.J., Gilbert R., Morgan P., Eliot Randle E., Schofield N., Varley P.G., Fisher J., Jonathan P., Waltho J.P., Hunter M.G.
    J. Biol. Chem. 274:16077-16084(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 24-92, MUTAGENESIS OF ASP-49 AND GLU-89.

Entry informationi

Entry nameiCCL3_HUMAN
AccessioniPrimary (citable) accession number: P10147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 3, 2014
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi