Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Interleukin-8

Gene

CXCL8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.3 Publications

GO - Molecular functioni

  • chemokine activity Source: UniProtKB
  • interleukin-8 receptor binding Source: UniProtKB

GO - Biological processi

  • angiogenesis Source: UniProtKB
  • antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
  • calcium-mediated signaling Source: UniProtKB
  • cell cycle arrest Source: UniProtKB
  • cellular response to fibroblast growth factor stimulus Source: UniProtKB
  • cellular response to interleukin-1 Source: UniProtKB
  • cellular response to lipopolysaccharide Source: BHF-UCL
  • cellular response to tumor necrosis factor Source: UniProtKB
  • chemokine-mediated signaling pathway Source: GO_Central
  • chemotaxis Source: UniProtKB
  • cytokine-mediated signaling pathway Source: Reactome
  • embryonic digestive tract development Source: DFLAT
  • G-protein coupled receptor signaling pathway Source: Reactome
  • immune response Source: GO_Central
  • induction of positive chemotaxis Source: UniProtKB
  • inflammatory response Source: UniProtKB
  • intracellular signal transduction Source: UniProtKB
  • killing of cells of other organism Source: UniProtKB
  • negative regulation of cell proliferation Source: ProtInc
  • negative regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  • neutrophil activation Source: UniProtKB
  • neutrophil chemotaxis Source: UniProtKB
  • PERK-mediated unfolded protein response Source: Reactome
  • positive regulation of angiogenesis Source: CACAO
  • positive regulation of neutrophil chemotaxis Source: BHF-UCL
  • receptor internalization Source: UniProtKB
  • regulation of cell adhesion Source: UniProtKB
  • regulation of entry of bacterium into host cell Source: AgBase
  • regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
  • response to endoplasmic reticulum stress Source: UniProtKB
  • response to molecule of bacterial origin Source: BHF-UCL
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionCytokine
Biological processChemotaxis, Inflammatory response

Enzyme and pathway databases

ReactomeiR-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-380108 Chemokine receptors bind chemokines
R-HSA-380994 ATF4 activates genes
R-HSA-418594 G alpha (i) signalling events
R-HSA-6783783 Interleukin-10 signaling
R-HSA-6785807 Interleukin-4 and 13 signaling
SIGNORiP10145

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-8
Short name:
IL-8
Alternative name(s):
C-X-C motif chemokine 8
Chemokine (C-X-C motif) ligand 8
Emoctakin
Granulocyte chemotactic protein 1
Short name:
GCP-1
Monocyte-derived neutrophil chemotactic factor
Short name:
MDNCF
Monocyte-derived neutrophil-activating peptide
Short name:
MONAP
Neutrophil-activating protein 1
Short name:
NAP-1
Protein 3-10C
T-cell chemotactic factor
Cleaved into the following 7 chains:
Alternative name(s):
GCP/IL-8 protein IV
IL8/NAP1 form I
Alternative name(s):
(Ala-IL-8)77
GCP/IL-8 protein II
IL-8(1-77)
IL8/NAP1 form II
MDNCF-b
Alternative name(s):
(Ser-IL-8)72
GCP/IL-8 protein I
IL8/NAP1 form III
Lymphocyte-derived neutrophil-activating factor
Short name:
LYNAP
MDNCF-c
Neutrophil-activating factor
Short name:
NAF
Alternative name(s):
GCP/IL-8 protein V
IL8/NAP1 form IV
Alternative name(s):
GCP/IL-8 protein VI
IL8/NAP1 form V
Alternative name(s):
GCP/IL-8 protein III
IL8/NAP1 form VI
Gene namesi
Name:CXCL8
Synonyms:IL8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000169429.10
HGNCiHGNC:6025 CXCL8
MIMi146930 gene
neXtProtiNX_P10145

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi3576
OpenTargetsiENSG00000169429
PharmGKBiPA29841

Chemistry databases

ChEMBLiCHEMBL2157
DrugBankiDB05434 ABT-510
DB05484 MDX-018
DB05855 Rivanicline

Polymorphism and mutation databases

BioMutaiIL8
DMDMi124359

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 203 PublicationsAdd BLAST20
ChainiPRO_000000512621 – 99MDNCF-aAdd BLAST79
ChainiPRO_000000512723 – 99Interleukin-8Add BLAST77
ChainiPRO_000004194827 – 99IL-8(5-77)Add BLAST73
ChainiPRO_000000512828 – 99IL-8(6-77)Add BLAST72
ChainiPRO_000000512929 – 99IL-8(7-77)Add BLAST71
ChainiPRO_000000513030 – 99IL-8(8-77)Add BLAST70
ChainiPRO_000000513131 – 99IL-8(9-77)Add BLAST69

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27Citrulline2 Publications1
Disulfide bondi34 ↔ 61
Disulfide bondi36 ↔ 77

Post-translational modificationi

Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.6 Publications
Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei27 – 28Cleavage; by thrombinCurated2
Sitei28 – 29Cleavage; by MMP92

Keywords - PTMi

Citrullination, Disulfide bond

Proteomic databases

PaxDbiP10145
PeptideAtlasiP10145
PRIDEiP10145
ProteomicsDBi52570

Miscellaneous databases

PMAP-CutDBiP10145

Expressioni

Inductioni

By ER stress in a DDIT3/CHOP-dependent manner.1 Publication

Gene expression databases

BgeeiENSG00000169429
CleanExiHS_IL8
ExpressionAtlasiP10145 baseline and differential
GenevisibleiP10145 HS

Organism-specific databases

HPAiHPA057179

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

GO - Molecular functioni

  • chemokine activity Source: UniProtKB
  • interleukin-8 receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109790, 13 interactors
DIPiDIP-3778N
IntActiP10145, 10 interactors
STRINGi9606.ENSP00000306512

Chemistry databases

BindingDBiP10145

Structurei

Secondary structure

199
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 42Combined sources6
Helixi46 – 48Combined sources3
Beta strandi49 – 55Combined sources7
Beta strandi58 – 60Combined sources3
Beta strandi61 – 63Combined sources3
Beta strandi65 – 70Combined sources6
Turni71 – 73Combined sources3
Beta strandi75 – 78Combined sources4
Helixi83 – 97Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ICWX-ray2.01A/B28-99[»]
1IKLNMR-A28-99[»]
1IKMNMR-A28-99[»]
1IL8NMR-A/B28-99[»]
1ILPNMR-A/B28-99[»]
1ILQNMR-A/B28-99[»]
1QE6X-ray2.35A/B/C/D28-99[»]
1RODNMR-A/B28-80[»]
2IL8NMR-A/B28-99[»]
3IL8X-ray2.00A28-99[»]
4XDXX-ray0.95A30-99[»]
5D14X-ray1.00A30-99[»]
5WDZNMR-A28-93[»]
ProteinModelPortaliP10145
SMRiP10145
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10145

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J24U Eukaryota
ENOG410Z1BP LUCA
GeneTreeiENSGT00530000062901
HOVERGENiHBG107789
InParanoidiP10145
KOiK10030
OMAiPTAPWVQ
OrthoDBiEOG091G13J8
PhylomeDBiP10145
TreeFamiTF333433

Family and domain databases

CDDicd00273 Chemokine_CXC, 1 hit
InterProiView protein in InterPro
IPR001089 Chemokine_CXC
IPR018048 Chemokine_CXC_CS
IPR001811 Chemokine_IL8-like_dom
IPR033899 CXC_Chemokine_domain
IPR028469 Interleukin-8
IPR036048 Interleukin_8-like_sf
PANTHERiPTHR10179 PTHR10179, 1 hit
PTHR10179:SF70 PTHR10179:SF70, 1 hit
PfamiView protein in Pfam
PF00048 IL8, 1 hit
PRINTSiPR00437 SMALLCYTKCXC
SMARTiView protein in SMART
SM00199 SCY, 1 hit
SUPFAMiSSF54117 SSF54117, 1 hit
PROSITEiView protein in PROSITE
PS00471 SMALL_CYTOKINES_CXC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK
60 70 80 90
ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
Length:99
Mass (Da):11,098
Last modified:July 1, 1989 - v1
Checksum:i15C649996E89319F
GO

Sequence cautioni

The sequence AAK00048 differs from that shown. Chimeric cDNA.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53R → L AA sequence (PubMed:2659722).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17017 mRNA Translation: AAA35611.1
Y00787 mRNA Translation: CAA68742.1
M28130 Genomic DNA Translation: AAA59158.1
M26383 mRNA Translation: AAA36323.1
D14283 Genomic DNA Translation: BAA03245.1
BT007067 mRNA Translation: AAP35730.1
AK311874 mRNA Translation: BAG34815.1
CR542151 mRNA Translation: CAG46948.1
AF385628 Genomic DNA Translation: AAK60276.1 Sequence problems.
CH471057 Genomic DNA Translation: EAX05688.1
BC013615 mRNA Translation: AAH13615.1
Z11686 mRNA Translation: CAA77745.1
AF043337 mRNA Translation: AAK00048.1 Sequence problems.
CCDSiCCDS34005.1
PIRiA37034
RefSeqiNP_000575.1, NM_000584.3
UniGeneiHs.624

Genome annotation databases

EnsembliENST00000307407; ENSP00000306512; ENSG00000169429
GeneIDi3576
KEGGihsa:3576
UCSCiuc003hhe.3 human

Similar proteinsi

Entry informationi

Entry nameiIL8_HUMAN
AccessioniPrimary (citable) accession number: P10145
Secondary accession number(s): B2R4L8
, Q6FGF6, Q6LAE6, Q96RG6, Q9C077, Q9UCE1, Q9UCR8, Q9UCR9, Q9UCS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 20, 2018
This is version 211 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health