P10127 (ADH4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase 4
EC=1.1.1.1

Alternative name(s):
Alcohol dehydrogenase IV
Short name=ADHIV

Gene names

Name:	ADH4
Synonyms:	ZRG5
Ordered Locus Names:	YGL256W
ORF Names:	NRC465

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduces acetaldehyde to ethanol during glucose fermentation. Specific for ethanol. Shows drastically reduced activity towards primary alcohols from 4 carbon atoms upward. Isomers of aliphatic alcohol, as well as secondary alcohols and glycerol are not used at all. Ref.13
Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Zinc. May bind iron when zinc levels are limiting. Ref.5
Enzyme regulation	Inhibited by EDTA. Ref.5
Subunit structure	Homodimer. Ref.5
Subcellular location	Mitochondrion Ref.8.
Induction	Induced by transcription factor ZAP1 in response to zinc deficiency. Ref.5 Ref.6 Ref.7 Ref.10 Ref.12 Ref.13
Miscellaneous	While ADH4 is expressed at only low levels in laboratory strains, it is often highly expressed in brewing strains. Present with 125 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.
Biophysicochemical properties	Kinetic parameters: KM=59 mM for NAD Ref.5 KM=122 µM for NADH KM=2.83 mM for acetaldehyde KM=16.7 mM for ethanol pH dependence: Optimum pH is 8.3.
Sequence caution	The sequence CAA64131.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA96976.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fermentation Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.8. Source: SGD
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from direct assay Ref.5. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERJ5	P43613	1	EBI-2233,EBI-23020
SWI6	P09959	1	EBI-2233,EBI-18641
TAF6	P53040	1	EBI-2233,EBI-18876
UIP3	P39547	1	EBI-2233,EBI-20760

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Alcohol dehydrogenase 4		PRO_0000087817
Experimental info
	Sequence conflict	60	1	D → G in CAA29410. Ref.1
	Sequence conflict	88	1	Q → E in CAA29410. Ref.1
	Sequence conflict	310	1	F → C in CAA29410. Ref.1
	Sequence conflict	339	1	E → D in CAA29410. Ref.1
	Sequence conflict	348	1	E → D in CAA29410. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10127 [UniParc]. Last modified July 22, 2008. Version 3. Checksum: 7DFFD43830FB269B	FASTA	382	41,142
	10 20 30 40 50 60 MSSVTGFYIP PISFFGEGAL EETADYIKNK DYKKALIVTD PGIAAIGLSG RVQKMLEERD 70 80 90 100 110 120 LNVAIYDKTQ PNPNIANVTA GLKVLKEQNS EIVVSIGGGS AHDNAKAIAL LATNGGEIGD 130 140 150 160 170 180 YEGVNQSKKA ALPLFAINTT AGTASEMTRF TIISNEEKKI KMAIIDNNVT PAVAVNDPST 190 200 210 220 230 240 MFGLPPALTA ATGLDALTHC IEAYVSTASN PITDACALKG IDLINESLVA AYKDGKDKKA 250 260 270 280 290 300 RTDMCYAEYL AGMAFNNASL GYVHALAHQL GGFYHLPHGV CNAVLLPHVQ EANMQCPKAK 310 320 330 340 350 360 KRLGEIALHF GASQEDPEET IKALHVLNRT MNIPRNLKEL GVKTEDFEIL AEHAMHDACH 370 380 LTNPVQFTKE QVVAIIKKAY EY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homology of Saccharomyces cerevisiae ADH4 to an iron-activated alcohol dehydrogenase from Zymomonas mobilis." Williamson V.M., Paquin C.E. Mol. Gen. Genet. 209:374-381(1987) [PubMed: 2823079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed: 8972578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Overexpression, purification and properties of alcohol dehydrogenase IV from Saccharomyces cerevisiae." Drewke C., Ciriacy M. Biochim. Biophys. Acta 950:54-60(1988) [PubMed: 3282541] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, COFACTOR, ENZYME REGULATION.
[6]	"Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging." Yuan D.S. Genetics 156:45-58(2000) [PubMed: 10978274] [Abstract] Cited for: INDUCTION.
[7]	"Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast." Lyons T.J., Gasch A.P., Gaither L.A., Botstein D., Brown P.O., Eide D.J. Proc. Natl. Acad. Sci. U.S.A. 97:7957-7962(2000) [PubMed: 10884426] [Abstract] Cited for: INDUCTION, IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Repression of ADH1 and ADH3 during zinc deficiency by Zap1-induced intergenic RNA transcripts." Bird A.J., Gordon M., Eide D.J., Winge D.R. EMBO J. 25:5726-5734(2006) [PubMed: 17139254] [Abstract] Cited for: INDUCTION.
[11]	"Characterization of low-acetic-acid-producing yeast isolated from 2-deoxyglucose-resistant mutants and its application to high-gravity brewing." Mizuno A., Tabei H., Iwahuti M. J. Biosci. Bioeng. 101:31-37(2006) [PubMed: 16503288] [Abstract] Cited for: EXPRESSION LEVEL IN BREWING STRAINS.
[12]	"Physiological and transcriptional responses of Saccharomyces cerevisiae to zinc limitation in chemostat cultures." De Nicola R., Hazelwood L.A., De Hulster E.A.F., Walsh M.C., Knijnenburg T.A., Reinders M.J.T., Walker G.M., Pronk J.T., Daran J.-M., Daran-Lapujade P. Appl. Environ. Microbiol. 73:7680-7692(2007) [PubMed: 17933919] [Abstract] Cited for: INDUCTION.
[13]	"Acetaldehyde addition throughout the growth phase alleviates the phenotypic effect of zinc deficiency in Saccharomyces cerevisiae." Cheraiti N., Sauvage F.-X., Salmon J.-M. Appl. Microbiol. Biotechnol. 77:1093-1109(2008) [PubMed: 17938904] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05992 Genomic DNA. Translation: CAA29410.1. X94357 Genomic DNA. Translation: CAA64131.1. Different initiation. Z72778 Genomic DNA. Translation: CAA96976.1. Different initiation. BK006941 Genomic DNA. Translation: DAA07863.1.
PIR	DEBY4. S61605.
RefSeq	NP_011258.2. NM_001181122.1.
3D structure databases
ProteinModelPortal	P10127.
SMR	P10127. Positions 5-380.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4812N.
IntAct	P10127. 11 interactions.
MINT	MINT-475922.
STRING	P10127.
Proteomic databases
PeptideAtlas	P10127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL256W; YGL256W; YGL256W.
GeneID	852636.
KEGG	sce:YGL256W.
NMPDR	fig|4932.3.peg.2361.
Organism-specific databases
CYGD	YGL256w.
SGD	S000003225. ADH4.
Phylogenomic databases
eggNOG	fuNOG07707.
GeneTree	EFGT00050000001042.
HOGENOM	HBG742415.
OMA	SAHDNAK.
OrthoDB	EOG4SXRN9.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11725.
Gene expression databases
ArrayExpress	P10127.
Genevestigator	P10127.
GermOnline	YGL256W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
KO	K13954.
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH4_YEAST

Accession

Primary (citable) accession number: P10127
Secondary accession number(s): D6VV79

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 22, 2008
Last modified:	November 16, 2011
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program