UniProtKB - P10126 (EF1A1_MOUSE)
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Protein
Elongation factor 1-alpha 1
Gene
Eef1a1
Organism
Mus musculus (Mouse)
Status
Functioni
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 14 – 21 | GTPBy similarity | 8 | |
Nucleotide bindingi | 91 – 95 | GTPBy similarity | 5 | |
Nucleotide bindingi | 153 – 156 | GTPBy similarity | 4 |
GO - Molecular functioni
- calmodulin binding Source: CAFA
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- protein kinase binding Source: MGI
- translation elongation factor activity Source: GO_Central
- tRNA binding Source: MGI
GO - Biological processi
- cellular response to epidermal growth factor stimulus Source: UniProtKB
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-156842. Eukaryotic Translation Elongation. R-MMU-3371511. HSF1 activation. R-MMU-6798695. Neutrophil degranulation. R-MMU-8876725. Protein methylation. |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 1-alpha 1Short name: EF-1-alpha-1 Alternative name(s): Elongation factor Tu Short name: EF-Tu Eukaryotic elongation factor 1 A-1 Short name: eEF1A-1 |
Gene namesi | Name:Eef1a1 Synonyms:Eef1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096881. Eef1a1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000090886 | 2 – 462 | Elongation factor 1-alpha 1Add BLAST | 461 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N,N,N-trimethylglycineBy similarity | 1 | |
Modified residuei | 36 | N6,N6,N6-trimethyllysine; alternateBy similarity | 1 | |
Modified residuei | 36 | N6,N6-dimethyllysine; alternateBy similarity | 1 | |
Modified residuei | 36 | N6-methyllysine; alternateBy similarity | 1 | |
Modified residuei | 55 | N6,N6-dimethyllysineBy similarity | 1 | |
Modified residuei | 79 | N6,N6,N6-trimethyllysine; by EEF1AKMT1By similarity | 1 | |
Modified residuei | 165 | N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT31 Publication | 1 | |
Modified residuei | 165 | N6,N6-dimethyllysine; alternate; by EEF1AKMT3By similarity | 1 | |
Modified residuei | 165 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 165 | N6-methyllysine; alternate; by EEF1AKMT3By similarity | 1 | |
Modified residuei | 172 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 273 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 300 | Phosphoserine; by TGFBR1By similarity | 1 | |
Modified residuei | 301 | 5-glutamyl glycerylphosphorylethanolamine1 Publication | 1 | |
Modified residuei | 318 | N6,N6,N6-trimethyllysine; by EEF1AKMT2By similarity | 1 | |
Modified residuei | 374 | 5-glutamyl glycerylphosphorylethanolamine1 Publication | 1 | |
Modified residuei | 392 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 392 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 432 | Phosphothreonine; by PASKBy similarity | 1 | |
Modified residuei | 439 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
ISGylated.1 Publication
Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2.By similarity
Trimethylated at Lys-79 by EEF1AKMT1 (By similarity). Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation (PubMed:28108655). Trimethylated at Lys-318 by EEF1AKMT2 (By similarity). Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (By similarity).By similarity1 Publication
Keywords - PTMi
Acetylation, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P10126. |
PaxDbi | P10126. |
PeptideAtlasi | P10126. |
PRIDEi | P10126. |
TopDownProteomicsi | P10126. |
2D gel databases
SWISS-2DPAGEi | P10126. |
PTM databases
iPTMneti | P10126. |
PhosphoSitePlusi | P10126. |
SwissPalmi | P10126. |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000037742. |
ExpressionAtlasi | P10126. baseline and differential. |
Genevisiblei | P10126. MM. |
Interactioni
Subunit structurei
Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS. May interact with ERGIC2 (By similarity). Interacts with IFIT1 (via TPR repeats 4-7) (PubMed:19856081). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation. Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner (By similarity). Interacts with PPP1R16B (By similarity).By similarity1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
TSPY1 | Q01534 | 5 | EBI-773865,EBI-1973142 | From Homo sapiens. |
GO - Molecular functioni
- calmodulin binding Source: CAFA
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGridi | 199385. 54 interactors. |
DIPi | DIP-46609N. |
IntActi | P10126. 65 interactors. |
MINTi | P10126. |
STRINGi | 10090.ENSMUSP00000042457. |
Structurei
3D structure databases
ProteinModelPortali | P10126. |
SMRi | P10126. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 242 | tr-type GAdd BLAST | 238 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 14 – 21 | G1By similarity | 8 | |
Regioni | 70 – 74 | G2By similarity | 5 | |
Regioni | 91 – 94 | G3By similarity | 4 | |
Regioni | 153 – 156 | G4By similarity | 4 | |
Regioni | 194 – 196 | G5By similarity | 3 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0052. Eukaryota. COG5256. LUCA. |
GeneTreei | ENSGT00910000144046. |
HOGENOMi | HOG000229291. |
HOVERGENi | HBG000179. |
InParanoidi | P10126. |
KOi | K03231. |
OMAi | PAKETKM. |
OrthoDBi | EOG091G05LW. |
PhylomeDBi | P10126. |
TreeFami | TF300304. |
Family and domain databases
HAMAPi | MF_00118_A. EF_Tu_A. 1 hit. |
InterProi | View protein in InterPro IPR004161. EFTu-like_2. IPR031157. G_TR_CS. IPR027417. P-loop_NTPase. IPR000795. TF_GTP-bd_dom. IPR009000. Transl_B-barrel_sf. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004539. Transl_elong_EF1A_euk/arc. IPR004160. Transl_elong_EFTu/EF1A_C. |
Pfami | View protein in Pfam PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. |
PRINTSi | PR00315. ELONGATNFCT. |
SUPFAMi | SSF50447. SSF50447. 1 hit. SSF50465. SSF50465. 1 hit. SSF52540. SSF52540. 1 hit. |
TIGRFAMsi | TIGR00483. EF-1_alpha. 1 hit. |
PROSITEi | View protein in PROSITE PS00301. G_TR_1. 1 hit. PS51722. G_TR_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P10126-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GSASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 | H → R in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 15 | H → L in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 23 | T → S in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 77 – 78 | LW → QR in CAA31957 (PubMed:2911475).Curated | 2 | |
Sequence conflicti | 83 | S → A in CAA27324 (PubMed:3960725).Curated | 1 | |
Sequence conflicti | 91 – 92 | DA → ES in CAA31957 (PubMed:2911475).Curated | 2 | |
Sequence conflicti | 108 | Q → R in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 156 | D → G in BAC28085 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 222 | S → H in AAA50406 (PubMed:3215517).Curated | 1 | |
Sequence conflicti | 224 – 226 | SGT → VAP in CAA31957 (PubMed:2911475).Curated | 3 | |
Sequence conflicti | 225 | G → D in AAH04005 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 239 | Missing in CAA31957 (PubMed:2911475).Curated | 1 | |
Sequence conflicti | 350 | P → T in BAC36446 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22432 mRNA. Translation: AAA50406.1. X13661 mRNA. Translation: CAA31957.1. AK032914 mRNA. Translation: BAC28085.1. AK076696 mRNA. Translation: BAC36446.1. AK081725 mRNA. Translation: BAC38311.1. AK083361 mRNA. Translation: BAC38884.1. BC004005 mRNA. Translation: AAH04005.1. BC004067 mRNA. Translation: AAH04067.1. BC005660 mRNA. Translation: AAH05660.1. BC018223 mRNA. Translation: AAH18223.1. BC018485 mRNA. Translation: AAH18485.1. BC083069 mRNA. Translation: AAH83069.1. M17878 Genomic DNA. Translation: AAA37538.1. X03688 mRNA. Translation: CAA27324.1. |
CCDSi | CCDS40703.1. |
PIRi | S02114. EFMS1. |
RefSeqi | NP_034236.2. NM_010106.2. |
UniGenei | Mm.138471. Mm.335315. Mm.360075. Mm.391071. Mm.485669. Mm.491424. |
Genome annotation databases
Ensembli | ENSMUST00000042235; ENSMUSP00000042457; ENSMUSG00000037742. |
GeneIDi | 13627. |
KEGGi | mmu:13627. |
UCSCi | uc009qun.1. mouse. |
Similar proteinsi
Entry informationi
Entry namei | EF1A1_MOUSE | |
Accessioni | P10126Primary (citable) accession number: P10126 Secondary accession number(s): Q61511 Q99KU5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 25, 2004 | |
Last modified: | March 28, 2018 | |
This is version 196 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |