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Protein

Annexin A1

Gene

Anxa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

GO - Molecular functioni

  1. annealing helicase activity Source: Ensembl
  2. calcium-dependent phospholipid binding Source: MGI
  3. calcium-dependent protein binding Source: MGI
  4. calcium ion binding Source: InterPro
  5. double-stranded DNA-dependent ATPase activity Source: Ensembl
  6. helicase activity Source: Ensembl
  7. phospholipase A2 inhibitor activity Source: MGI
  8. protein binding, bridging Source: MGI
  9. single-stranded DNA binding Source: Ensembl
  10. single-stranded RNA binding Source: Ensembl
  11. structural molecule activity Source: MGI

GO - Biological processi

  1. alpha-beta T cell differentiation Source: BHF-UCL
  2. arachidonic acid secretion Source: MGI
  3. cell surface receptor signaling pathway Source: Ensembl
  4. cellular response to glucocorticoid stimulus Source: MGI
  5. cellular response to hydrogen peroxide Source: Ensembl
  6. DNA duplex unwinding Source: Ensembl
  7. DNA rewinding Source: Ensembl
  8. endocrine pancreas development Source: Ensembl
  9. gliogenesis Source: Ensembl
  10. hepatocyte differentiation Source: Ensembl
  11. insulin secretion Source: Ensembl
  12. keratinocyte differentiation Source: MGI
  13. negative regulation of interleukin-8 secretion Source: MGI
  14. negative regulation of phospholipase A2 activity Source: Ensembl
  15. neutrophil clearance Source: MGI
  16. neutrophil homeostasis Source: BHF-UCL
  17. peptide cross-linking Source: MGI
  18. positive regulation of G1/S transition of mitotic cell cycle Source: MGI
  19. positive regulation of neutrophil apoptotic process Source: BHF-UCL
  20. positive regulation of prostaglandin biosynthetic process Source: Ensembl
  21. positive regulation of vesicle fusion Source: MGI
  22. prolactin secretion Source: Ensembl
  23. prostate gland development Source: Ensembl
  24. regulation of cell proliferation Source: MGI
  25. response to drug Source: Ensembl
  26. response to estradiol Source: Ensembl
  27. response to interleukin-1 Source: Ensembl
  28. response to peptide hormone Source: Ensembl
  29. response to X-ray Source: Ensembl
  30. signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Phospholipase A2 inhibitor

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Enzyme and pathway databases

ReactomeiREACT_272716. Formyl peptide receptors bind formyl peptides and many other ligands.
REACT_331048. G alpha (i) signalling events.
REACT_347934. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A1
Alternative name(s):
Annexin I
Annexin-1
Calpactin II
Calpactin-2
Chromobindin-9
Lipocortin I
Phospholipase A2 inhibitory protein
p35
Gene namesi
Name:Anxa1
Synonyms:Anx1, Lpc-1, Lpc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:96819. Anxa1.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Cell projectioncilium By similarity. Basolateral cell membrane By similarity
Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages.By similarity

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. cell surface Source: MGI
  3. cilium Source: UniProtKB-SubCell
  4. cornified envelope Source: MGI
  5. cytoplasm Source: MGI
  6. endosome Source: MGI
  7. extracellular space Source: MGI
  8. extracellular vesicular exosome Source: MGI
  9. focal adhesion Source: MGI
  10. mast cell granule Source: Ensembl
  11. mitochondrial membrane Source: Ensembl
  12. nucleus Source: MGI
  13. plasma membrane Source: MGI
  14. protein complex Source: Ensembl
  15. sarcolemma Source: MGI
  16. vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 346345Annexin A1PRO_0000067461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei5 – 51Phosphoserine; by TRPM7By similarity
Cross-linki19 – 19Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-?)By similarity
Modified residuei21 – 211Phosphotyrosine1 Publication
Modified residuei27 – 271Phosphoserine; by PKCBy similarity
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei58 – 581N6-acetyllysine1 Publication
Modified residuei312 – 3121N6-acetyllysine1 Publication

Post-translational modificationi

Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein

Proteomic databases

MaxQBiP10107.
PaxDbiP10107.
PRIDEiP10107.

2D gel databases

COMPLUYEAST-2DPAGEP10107.

PTM databases

PhosphoSiteiP10107.

Expressioni

Gene expression databases

BgeeiP10107.
CleanExiMM_ANXA1.
ExpressionAtlasiP10107. baseline and differential.
GenevestigatoriP10107.

Interactioni

Subunit structurei

Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF.1 Publication

Protein-protein interaction databases

BioGridi201194. 4 interactions.
IntActiP10107. 6 interactions.
MINTiMINT-274958.

Structurei

3D structure databases

ProteinModelPortaliP10107.
SMRiP10107. Positions 2-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati51 – 11161Annexin 1Add
BLAST
Repeati123 – 18361Annexin 2Add
BLAST
Repeati207 – 26761Annexin 3Add
BLAST
Repeati282 – 34261Annexin 4Add
BLAST

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiNOG282829.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP10107.
KOiK17091.
OMAiMNDIKAC.
OrthoDBiEOG74XS72.
PhylomeDBiP10107.
TreeFamiTF105452.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002388. AnnexinI.
[Graphical view]
PANTHERiPTHR10502:SF17. PTHR10502:SF17. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00197. ANNEXINI.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMVSEFLKQ ARFLENQEQE YVQAVKSYKG GPGSAVSPYP SFNVSSDVAA
60 70 80 90 100
LHKAIMVKGV DEATIIDILT KRTNAQRQQI KAAYLQENGK PLDEVLRKAL
110 120 130 140 150
TGHLEEVVLA MLKTPAQFDA DELRGAMKGL GTDEDTLIEI LTTRSNEQIR
160 170 180 190 200
EINRVYREEL KRDLAKDITS DTSGDFRKAL LALAKGDRCQ DLSVNQDLAD
210 220 230 240 250
TDARALYEAG ERRKGTDVNV FTTILTSRSF PHLRRVFQNY GKYSQHDMNK
260 270 280 290 300
ALDLELKGDI EKCLTTIVKC ATSTPAFFAE KLYEAMKGAG TRHKALIRIM
310 320 330 340
VSRSEIDMNE IKVFYQKKYG ISLCQAILDE TKGDYEKILV ALCGGN
Length:346
Mass (Da):38,734
Last modified:January 22, 2007 - v2
Checksum:i9393A11B24191D3C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 792QQ → PR in AAA39420 (PubMed:2522299).Curated
Sequence conflicti212 – 2121R → I in AAA39437 (PubMed:1676980).Curated
Sequence conflicti222 – 2221T → H in AAA39420 (PubMed:2522299).Curated
Sequence conflicti274 – 2741T → H in AAA39420 (PubMed:2522299).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07486 mRNA. Translation: CAA30371.1.
M69260
, M69250, M69251, M69252, M69253, M69254, M69255, M69256, M69257, M69258, M69259 Genomic DNA. Translation: AAA39437.1.
BC002289 mRNA. Translation: AAH02289.1.
BC004594 mRNA. Translation: AAH04594.1.
M24554 mRNA. Translation: AAA39420.1.
CCDSiCCDS29692.1.
PIRiS02181. LUMS1.
RefSeqiNP_034860.2. NM_010730.2.
UniGeneiMm.248360.

Genome annotation databases

EnsembliENSMUST00000025561; ENSMUSP00000025561; ENSMUSG00000024659.
GeneIDi16952.
KEGGimmu:16952.
UCSCiuc008gyi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07486 mRNA. Translation: CAA30371.1.
M69260
, M69250, M69251, M69252, M69253, M69254, M69255, M69256, M69257, M69258, M69259 Genomic DNA. Translation: AAA39437.1.
BC002289 mRNA. Translation: AAH02289.1.
BC004594 mRNA. Translation: AAH04594.1.
M24554 mRNA. Translation: AAA39420.1.
CCDSiCCDS29692.1.
PIRiS02181. LUMS1.
RefSeqiNP_034860.2. NM_010730.2.
UniGeneiMm.248360.

3D structure databases

ProteinModelPortaliP10107.
SMRiP10107. Positions 2-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201194. 4 interactions.
IntActiP10107. 6 interactions.
MINTiMINT-274958.

PTM databases

PhosphoSiteiP10107.

2D gel databases

COMPLUYEAST-2DPAGEP10107.

Proteomic databases

MaxQBiP10107.
PaxDbiP10107.
PRIDEiP10107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025561; ENSMUSP00000025561; ENSMUSG00000024659.
GeneIDi16952.
KEGGimmu:16952.
UCSCiuc008gyi.1. mouse.

Organism-specific databases

CTDi301.
MGIiMGI:96819. Anxa1.

Phylogenomic databases

eggNOGiNOG282829.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP10107.
KOiK17091.
OMAiMNDIKAC.
OrthoDBiEOG74XS72.
PhylomeDBiP10107.
TreeFamiTF105452.

Enzyme and pathway databases

ReactomeiREACT_272716. Formyl peptide receptors bind formyl peptides and many other ligands.
REACT_331048. G alpha (i) signalling events.
REACT_347934. G alpha (q) signalling events.

Miscellaneous databases

ChiTaRSiAnxa1. mouse.
NextBioi291004.
PROiP10107.
SOURCEiSearch...

Gene expression databases

BgeeiP10107.
CleanExiMM_ANXA1.
ExpressionAtlasiP10107. baseline and differential.
GenevestigatoriP10107.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002388. AnnexinI.
[Graphical view]
PANTHERiPTHR10502:SF17. PTHR10502:SF17. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00197. ANNEXINI.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mouse lipocortin I gene structure and chromosomal assignment: gene duplication and the origins of a gene family."
    Horlick K.R., Cheng I.C., Wong W.T., Wakeland E.K., Nick H.S.
    Genomics 10:365-374(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "cDNA-cloning, sequencing and expression in glucocorticoid-stimulated quiescent Swiss 3T3 fibroblasts of mouse lipocortin I."
    Philipps C., Rose-John S., Rincke G., Fuerstenberger G., Marks F.
    Biochem. Biophys. Res. Commun. 159:155-162(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-346.
  5. "Dysferlin interacts with annexins A1 and A2 and mediates sarcolemmal wound-healing."
    Lennon N.J., Kho A., Bacskai B.J., Perlmutter S.L., Hyman B.T., Brown R.H. Jr.
    J. Biol. Chem. 278:50466-50473(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYSF.
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-58 AND LYS-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiANXA1_MOUSE
AccessioniPrimary (citable) accession number: P10107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1989
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.