P10107 (ANXA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Annexin A1

Alternative name(s):
Annexin I
Annexin-1
Calpactin II
Calpactin-2
Chromobindin-9
Lipocortin I
Phospholipase A2 inhibitory protein
p35

Gene names

Name:	Anxa1
Synonyms:	Anx1, Lpc-1, Lpc1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Subunit structure	Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF. Ref.5
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cell projection › cilium By similarity. Basolateral cell membrane By similarity. Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium By similarity. Found in the cytoplasm of type II pneumocytes and alveolar macrophages By similarity.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modification	Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity By similarity.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cilium Cytoplasm Membrane Nucleus
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
Molecular function	Phospholipase A2 inhibitor
PTM	Acetylation Isopeptide bond Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	alpha-beta T cell differentiation Inferred from mutant phenotype PubMed 20139728. Source: BHF-UCL arachidonic acid secretion Inferred from mutant phenotype PubMed 12553880. Source: MGI cell cycle Inferred from mutant phenotype PubMed 12553880. Source: MGI cell surface receptor signaling pathway Inferred from electronic annotation. Source: Compara cellular response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara cellular response to hydrogen peroxide Inferred from electronic annotation. Source: Compara endocrine pancreas development Inferred from electronic annotation. Source: Compara estrous cycle phase Inferred from electronic annotation. Source: Compara gliogenesis Inferred from electronic annotation. Source: Compara hepatocyte differentiation Inferred from electronic annotation. Source: Compara insulin secretion Inferred from electronic annotation. Source: Compara keratinocyte differentiation Inferred from electronic annotation. Source: Compara negative regulation of acute inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of protein secretion Inferred from electronic annotation. Source: Compara neutrophil clearance Inferred from electronic annotation. Source: Compara neutrophil homeostasis Inferred from mutant phenotype PubMed 21957127. Source: BHF-UCL peptide cross-linking Inferred from electronic annotation. Source: Compara positive regulation of neutrophil apoptotic process Inferred from mutant phenotype PubMed 21957127. Source: BHF-UCL positive regulation of prostaglandin biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of vesicle fusion Inferred from electronic annotation. Source: Compara regulation of cell proliferation Inferred from mutant phenotype PubMed 12553880. Source: MGI response to X-ray Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to interleukin-1 Inferred from electronic annotation. Source: Compara response to peptide hormone stimulus Inferred from electronic annotation. Source: Compara signal transduction Inferred from mutant phenotype PubMed 12553880. Source: MGI
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cilium Inferred from electronic annotation. Source: UniProtKB-SubCell cornified envelope Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara mitochondrial membrane Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from electronic annotation. Source: Compara sarcolemma Inferred from direct assay Ref.5. Source: MGI
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 346	345	Annexin A1		PRO_0000067461
Regions
	Repeat	51 – 111	61	Annexin 1
	Repeat	123 – 183	61	Annexin 2
	Repeat	207 – 267	61	Annexin 3
	Repeat	282 – 342	61	Annexin 4
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	5	1	Phosphoserine; by TRPM7 By similarity
	Modified residue	21	1	Phosphotyrosine Ref.6
	Modified residue	27	1	Phosphoserine; by PKC By similarity
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	312	1	N6-acetyllysine By similarity
	Cross-link	19		Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-?) By similarity
Experimental info
	Sequence conflict	78 – 79	2	QQ → PR in AAA39420. Ref.4
	Sequence conflict	212	1	R → I in AAA39437. Ref.2
	Sequence conflict	222	1	T → H in AAA39420. Ref.4
	Sequence conflict	274	1	T → H in AAA39420. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P10107 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9393A11B24191D3C	FASTA	346	38,734
	10 20 30 40 50 60 MAMVSEFLKQ ARFLENQEQE YVQAVKSYKG GPGSAVSPYP SFNVSSDVAA LHKAIMVKGV 70 80 90 100 110 120 DEATIIDILT KRTNAQRQQI KAAYLQENGK PLDEVLRKAL TGHLEEVVLA MLKTPAQFDA 130 140 150 160 170 180 DELRGAMKGL GTDEDTLIEI LTTRSNEQIR EINRVYREEL KRDLAKDITS DTSGDFRKAL 190 200 210 220 230 240 LALAKGDRCQ DLSVNQDLAD TDARALYEAG ERRKGTDVNV FTTILTSRSF PHLRRVFQNY 250 260 270 280 290 300 GKYSQHDMNK ALDLELKGDI EKCLTTIVKC ATSTPAFFAE KLYEAMKGAG TRHKALIRIM 310 320 330 340 VSRSEIDMNE IKVFYQKKYG ISLCQAILDE TKGDYEKILV ALCGGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse lipocortin I cDNA." Sakata T., Iwagami S., Tsuruta Y., Suzuki R., Hojo K., Sato K., Teraoka H. Nucleic Acids Res. 16:11818-11818(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mouse lipocortin I gene structure and chromosomal assignment: gene duplication and the origins of a gene family." Horlick K.R., Cheng I.C., Wong W.T., Wakeland E.K., Nick H.S. Genomics 10:365-374(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[4]	"cDNA-cloning, sequencing and expression in glucocorticoid-stimulated quiescent Swiss 3T3 fibroblasts of mouse lipocortin I." Philipps C., Rose-John S., Rincke G., Fuerstenberger G., Marks F. Biochem. Biophys. Res. Commun. 159:155-162(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-346.
[5]	"Dysferlin interacts with annexins A1 and A2 and mediates sarcolemmal wound-healing." Lennon N.J., Kho A., Bacskai B.J., Perlmutter S.L., Hyman B.T., Brown R.H. Jr. J. Biol. Chem. 278:50466-50473(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DYSF.
[6]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-21, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07486 mRNA. Translation: CAA30371.1. M69260 , M69250, M69251, M69252, M69253, M69254, M69255, M69256, M69257, M69258, M69259 Genomic DNA. Translation: AAA39437.1. BC002289 mRNA. Translation: AAH02289.1. BC004594 mRNA. Translation: AAH04594.1. M24554 mRNA. Translation: AAA39420.1.
IPI	IPI00230395.
PIR	LUMS1. S02181.
RefSeq	NP_034860.2. NM_010730.2.
UniGene	Mm.248360.
3D structure databases
ProteinModelPortal	P10107.
SMR	P10107. Positions 2-344.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10107. 4 interactions.
MINT	MINT-274958.
PTM databases
PhosphoSite	P10107.
2D gel databases
COMPLUYEAST-2DPAGE	P10107.
Proteomic databases
PaxDb	P10107.
PRIDE	P10107.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025561; ENSMUSP00000025561; ENSMUSG00000024659.
GeneID	16952.
KEGG	mmu:16952.
Organism-specific databases
CTD	301.
MGI	MGI:96819. Anxa1.
Phylogenomic databases
eggNOG	NOG282829.
HOGENOM	HOG000158803.
HOVERGEN	HBG061815.
OMA	GTRHKTL.
Gene expression databases
ArrayExpress	P10107.
Bgee	P10107.
CleanEx	MM_ANXA1.
Genevestigator	P10107.
GermOnline	ENSMUSG00000024659. Mus musculus.
Family and domain databases
Gene3D	1.10.220.10. 4 hits.
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR002388. AnnexinI. [Graphical view]
PANTHER	PTHR10502. PTHR10502. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00197. ANNEXINI.
SMART	SM00335. ANX. 4 hits. [Graphical view]
SUPFAM	SSF47874. Annexin. 1 hit.
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ANXA1. mouse.
NextBio	291004.
SOURCE	Search...

Entry information

Entry name

ANXA1_MOUSE

Accession

Primary (citable) accession number: P10107

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 133 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program