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Protein

Endolytic peptidoglycan transglycosylase RlpA

Gene

rlpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:EG10854-MONOMER.
ECOL316407:JW0628-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolytic peptidoglycan transglycosylase RlpAUniRule annotation (EC:4.2.2.-UniRule annotation)
Alternative name(s):
Rare lipoprotein A1 Publication
Gene namesi
Name:rlpA1 PublicationUniRule annotation
Ordered Locus Names:b0633, JW0628
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10854. rlpA.

Subcellular locationi

  • Cell membrane UniRule annotation; Lipid-anchor UniRule annotation1 Publication

  • Note: Localizes at the septal ring. Is also associated with some other structures/complexes along the cell cylinder.2 Publications

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion does not result in any obvious growth or division defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17UniRule annotation1 PublicationAdd BLAST17
ChainiPRO_000003079618 – 362Endolytic peptidoglycan transglycosylase RlpAUniRule annotationAdd BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi18N-palmitoyl cysteineUniRule annotation1 Publication1
Lipidationi18S-diacylglycerol cysteineUniRule annotation1 Publication1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP10100.
PRIDEiP10100.

Interactioni

Protein-protein interaction databases

BioGridi4260680. 169 interactors.
IntActiP10100. 1 interactor.
STRINGi511145.b0633.

Structurei

3D structure databases

ProteinModelPortaliP10100.
SMRiP10100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 361SPORUniRule annotationAdd BLAST77

Domaini

The SPOR domain binds septal peptidoglycans and is required to target RlpA to the septal ring.1 Publication

Sequence similaritiesi

Belongs to the RlpA family.UniRule annotation
Contains 1 SPOR domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105K6T. Bacteria.
COG0797. LUCA.
HOGENOMiHOG000117956.
InParanoidiP10100.
KOiK03642.
OMAiQSFIAVA.

Family and domain databases

Gene3Di3.30.70.1070. 1 hit.
HAMAPiMF_02071. RlpA. 1 hit.
InterProiIPR009009. RlpA-like_DPBB.
IPR012997. RplA.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF03330. DPBB_1. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
SSF50685. SSF50685. 1 hit.
TIGRFAMsiTIGR00413. rlpA. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS51724. SPOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKQWLGICI AAGMLAACTS DDGQQQTVSV PQPAVCNGPI VEISGADPRF
60 70 80 90 100
EPLNATANQD YQRDGKSYKI VQDPSRFSQA GLAAIYDAEP GSNLTASGEA
110 120 130 140 150
FDPTQLTAAH PTLPIPSYAR ITNLANGRMI VVRINDRGPY GNDRVISLSR
160 170 180 190 200
AAADRLNTSN NTKVRIDPII VAQDGSLSGP GMACTTVAKQ TYALPAPPDL
210 220 230 240 250
SGGAGTSSVS GPQGDILPVS NSTLKSEDPT GAPVTSSGFL GAPTTLAPGV
260 270 280 290 300
LEGSEPTPAP QPVVTAPSTT PATSPAMVTP QAVSQSASGN FMVQVGAVSD
310 320 330 340 350
QARAQQYQQQ LGQKFGVPGR VTQNGAVWRI QLGPFASKAE ASTLQQRLQT
360
EAQLQSFITT AQ
Length:362
Mass (Da):37,528
Last modified:July 1, 1989 - v1
Checksum:iE28B57023CF15686
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18276 Genomic DNA. Translation: AAA24552.1.
U82598 Genomic DNA. Translation: AAB40833.1.
U00096 Genomic DNA. Translation: AAC73734.1.
AP009048 Genomic DNA. Translation: BAA35276.1.
M22857 Genomic DNA. Translation: AAA24572.1.
PIRiA28387. LPECRA.
RefSeqiNP_415166.1. NC_000913.3.
WP_001231430.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73734; AAC73734; b0633.
BAA35276; BAA35276; BAA35276.
GeneIDi945241.
KEGGiecj:JW0628.
eco:b0633.
PATRICi32116449. VBIEscCol129921_0664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18276 Genomic DNA. Translation: AAA24552.1.
U82598 Genomic DNA. Translation: AAB40833.1.
U00096 Genomic DNA. Translation: AAC73734.1.
AP009048 Genomic DNA. Translation: BAA35276.1.
M22857 Genomic DNA. Translation: AAA24572.1.
PIRiA28387. LPECRA.
RefSeqiNP_415166.1. NC_000913.3.
WP_001231430.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP10100.
SMRiP10100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260680. 169 interactors.
IntActiP10100. 1 interactor.
STRINGi511145.b0633.

Proteomic databases

PaxDbiP10100.
PRIDEiP10100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73734; AAC73734; b0633.
BAA35276; BAA35276; BAA35276.
GeneIDi945241.
KEGGiecj:JW0628.
eco:b0633.
PATRICi32116449. VBIEscCol129921_0664.

Organism-specific databases

EchoBASEiEB0847.
EcoGeneiEG10854. rlpA.

Phylogenomic databases

eggNOGiENOG4105K6T. Bacteria.
COG0797. LUCA.
HOGENOMiHOG000117956.
InParanoidiP10100.
KOiK03642.
OMAiQSFIAVA.

Enzyme and pathway databases

BioCyciEcoCyc:EG10854-MONOMER.
ECOL316407:JW0628-MONOMER.

Miscellaneous databases

PROiP10100.

Family and domain databases

Gene3Di3.30.70.1070. 1 hit.
HAMAPiMF_02071. RlpA. 1 hit.
InterProiIPR009009. RlpA-like_DPBB.
IPR012997. RplA.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF03330. DPBB_1. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
SSF50685. SSF50685. 1 hit.
TIGRFAMsiTIGR00413. rlpA. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS51724. SPOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLPA_ECOLI
AccessioniPrimary (citable) accession number: P10100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.