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Protein

Myoblast determination protein 1

Gene

Myod1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.2 Publications

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • chromatin DNA binding Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • DNA binding Source: MGI
  • enzyme binding Source: BHF-UCL
  • nuclear hormone receptor binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: MGI
  • transcription coactivator activity Source: UniProtKB
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription factor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  • cardiac muscle cell differentiation Source: UniProtKB
  • cellular response to estradiol stimulus Source: UniProtKB
  • cellular response to glucocorticoid stimulus Source: MGI
  • cellular response to oxygen levels Source: MGI
  • cellular response to starvation Source: MGI
  • histone H3 acetylation Source: UniProtKB
  • histone H4 acetylation Source: UniProtKB
  • muscle organ development Source: MGI
  • myoblast differentiation Source: MGI
  • myoblast fate determination Source: MGI
  • myoblast fusion Source: MGI
  • myotube cell development Source: MGI
  • myotube differentiation Source: MGI
  • myotube differentiation involved in skeletal muscle regeneration Source: MGI
  • negative regulation of myoblast proliferation Source: MGI
  • positive regulation of muscle cell differentiation Source: MGI
  • positive regulation of myoblast differentiation Source: GO_Central
  • positive regulation of myoblast fusion Source: MGI
  • positive regulation of skeletal muscle fiber development Source: GO_Central
  • positive regulation of skeletal muscle tissue regeneration Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: MGI
  • regulation of gene expression Source: CACAO
  • regulation of RNA splicing Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • skeletal muscle cell differentiation Source: UniProtKB
  • skeletal muscle fiber adaptation Source: MGI
  • skeletal muscle fiber development Source: MGI
  • skeletal muscle tissue development Source: MGI
  • striated muscle cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-375170. CDO in myogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Myoblast determination protein 1
Gene namesi
Name:Myod1
Synonyms:Myod
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97275. Myod1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • myofibril Source: MGI
  • nuclear chromatin Source: BHF-UCL
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273611 – 318Myoblast determination protein 1Add BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei104N6-methyllysine; by EHMT2By similarity1

Post-translational modificationi

Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.1 Publication
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.By similarity
Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation (By similarity).By similarity
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP10085.
PRIDEiP10085.

PTM databases

iPTMnetiP10085.
PhosphoSitePlusiP10085.

Expressioni

Gene expression databases

BgeeiENSMUSG00000009471.
CleanExiMM_MYOD1.
GenevisibleiP10085. MM.

Interactioni

Subunit structurei

Interacts with SUV39H1 (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 isoform 1 and isoform 4. Interacts with SETD3 (PubMed:21832073). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (PubMed:12037670). Interacts with CSRP3 (By similarity).By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Csrp3P504632EBI-4405734,EBI-12502290From a different organism.
DDX5P178443EBI-4405734,EBI-351962From a different organism.
KAT5Q929935EBI-4405734,EBI-399080From a different organism.
Smarcd3Q6P9Z16EBI-4405734,EBI-7525857
Suv39h1O548643EBI-4405734,EBI-302230

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • nuclear hormone receptor binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • transcription factor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: MGI

Protein-protein interaction databases

BioGridi201673. 31 interactors.
DIPiDIP-37N.
IntActiP10085. 16 interactors.
MINTiMINT-263097.
STRINGi10090.ENSMUSP00000072330.

Structurei

Secondary structure

1318
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi106 – 133Combined sources28
Helixi146 – 162Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MDYX-ray2.80A102-166[»]
B/C/D105-166[»]
ProteinModelPortaliP10085.
SMRiP10085.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10085.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 160bHLHPROSITE-ProRule annotationAdd BLAST52

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3960. Eukaryota.
ENOG4111SED. LUCA.
GeneTreeiENSGT00530000063004.
HOGENOMiHOG000234800.
HOVERGENiHBG006429.
InParanoidiP10085.
KOiK09064.
OMAiDPRLMHV.
OrthoDBiEOG091G0GKD.
TreeFamiTF316344.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLSPPLRD IDLTGPDGSL CSFETADDFY DDPCFDSPDL RFFEDLDPRL
60 70 80 90 100
VHMGALLKPE EHAHFPTAVH PGPGAREDEH VRAPSGHHQA GRCLLWACKA
110 120 130 140 150
CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL
160 170 180 190 200
RNAIRYIEGL QALLRDQDAA PPGAAAFYAP GPLPPGRGSE HYSGDSDASS
210 220 230 240 250
PRSNCSDGMM DYSGPPSGPR RQNGYDTAYY SEAARESRPG KSAAVSSLDC
260 270 280 290 300
LSSIVERIST DSPAAPALLL ADAPPESPPG PPEGASLSDT EQGTQTPSPD
310
AAPQCPAGSN PNAIYQVL
Length:318
Mass (Da):34,233
Last modified:July 27, 2011 - v2
Checksum:iAEC6572277A78D54
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53M → V in AAA39799 (PubMed:3690668).Curated1
Sequence conflicti53M → V in CAA43836 (PubMed:1754380).Curated1
Sequence conflicti53M → V in AAA39798 (PubMed:3286015).Curated1
Sequence conflicti66P → S in AAA39799 (PubMed:3690668).Curated1
Sequence conflicti66P → S in CAA43836 (PubMed:1754380).Curated1
Sequence conflicti66P → S in AAA39798 (PubMed:3286015).Curated1
Sequence conflicti234A → V in AAA39799 (PubMed:3690668).Curated1
Sequence conflicti234A → V in CAA43836 (PubMed:1754380).Curated1
Sequence conflicti234A → V in AAA39798 (PubMed:3286015).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18779 mRNA. Translation: AAA39799.1.
X61655 Genomic DNA. Translation: CAA43836.1.
M84918 mRNA. Translation: AAA39798.1.
AK076157 mRNA. Translation: BAC36224.1.
AK142859 mRNA. Translation: BAE25213.1.
BC103613 mRNA. Translation: AAI03614.1.
BC103618 mRNA. Translation: AAI03619.1.
BC103619 mRNA. Translation: AAI03620.1.
CCDSiCCDS21277.1.
PIRiA29636.
RefSeqiNP_034996.2. NM_010866.2.
UniGeneiMm.1526.

Genome annotation databases

EnsembliENSMUST00000072514; ENSMUSP00000072330; ENSMUSG00000009471.
GeneIDi17927.
KEGGimmu:17927.
UCSCiuc012fkv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18779 mRNA. Translation: AAA39799.1.
X61655 Genomic DNA. Translation: CAA43836.1.
M84918 mRNA. Translation: AAA39798.1.
AK076157 mRNA. Translation: BAC36224.1.
AK142859 mRNA. Translation: BAE25213.1.
BC103613 mRNA. Translation: AAI03614.1.
BC103618 mRNA. Translation: AAI03619.1.
BC103619 mRNA. Translation: AAI03620.1.
CCDSiCCDS21277.1.
PIRiA29636.
RefSeqiNP_034996.2. NM_010866.2.
UniGeneiMm.1526.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MDYX-ray2.80A102-166[»]
B/C/D105-166[»]
ProteinModelPortaliP10085.
SMRiP10085.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201673. 31 interactors.
DIPiDIP-37N.
IntActiP10085. 16 interactors.
MINTiMINT-263097.
STRINGi10090.ENSMUSP00000072330.

PTM databases

iPTMnetiP10085.
PhosphoSitePlusiP10085.

Proteomic databases

PaxDbiP10085.
PRIDEiP10085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072514; ENSMUSP00000072330; ENSMUSG00000009471.
GeneIDi17927.
KEGGimmu:17927.
UCSCiuc012fkv.1. mouse.

Organism-specific databases

CTDi4654.
MGIiMGI:97275. Myod1.

Phylogenomic databases

eggNOGiKOG3960. Eukaryota.
ENOG4111SED. LUCA.
GeneTreeiENSGT00530000063004.
HOGENOMiHOG000234800.
HOVERGENiHBG006429.
InParanoidiP10085.
KOiK09064.
OMAiDPRLMHV.
OrthoDBiEOG091G0GKD.
TreeFamiTF316344.

Enzyme and pathway databases

ReactomeiR-MMU-375170. CDO in myogenesis.

Miscellaneous databases

EvolutionaryTraceiP10085.
PROiP10085.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000009471.
CleanExiMM_MYOD1.
GenevisibleiP10085. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYOD1_MOUSE
AccessioniPrimary (citable) accession number: P10085
Secondary accession number(s): Q8C6B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 171 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.