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Protein

Myoblast determination protein 1

Gene

Myod1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed:16901893). Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (PubMed:21798092, PubMed:3175662).3 Publications

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • chromatin DNA binding Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: MGI
  • enzyme binding Source: BHF-UCL
  • nuclear hormone receptor binding Source: MGI
  • promoter-specific chromatin binding Source: UniProtKB
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
  • transcription coactivator activity Source: UniProtKB
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI
  • transcription factor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  • cardiac muscle cell differentiation Source: UniProtKB
  • cellular response to estradiol stimulus Source: UniProtKB
  • cellular response to glucocorticoid stimulus Source: MGI
  • cellular response to oxygen levels Source: MGI
  • cellular response to starvation Source: MGI
  • cellular response to tumor necrosis factor Source: MGI
  • histone H3 acetylation Source: UniProtKB
  • histone H4 acetylation Source: UniProtKB
  • muscle organ development Source: MGI
  • myoblast differentiation Source: MGI
  • myoblast fate determination Source: MGI
  • myoblast fusion Source: MGI
  • myotube cell development Source: MGI
  • myotube differentiation Source: MGI
  • myotube differentiation involved in skeletal muscle regeneration Source: MGI
  • negative regulation of myoblast proliferation Source: MGI
  • positive regulation of muscle cell differentiation Source: UniProtKB
  • positive regulation of myoblast differentiation Source: GO_Central
  • positive regulation of myoblast fusion Source: MGI
  • positive regulation of skeletal muscle fiber development Source: GO_Central
  • positive regulation of skeletal muscle tissue regeneration Source: MGI
  • positive regulation of snRNA transcription by RNA polymerase II Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: MGI
  • regulation of gene expression Source: CACAO
  • regulation of RNA splicing Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • skeletal muscle cell differentiation Source: MGI
  • skeletal muscle fiber adaptation Source: MGI
  • skeletal muscle fiber development Source: MGI
  • skeletal muscle tissue development Source: MGI
  • striated muscle cell differentiation Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Myogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-375170 CDO in myogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Myoblast determination protein 1
Gene namesi
Name:Myod1
Synonyms:Myod
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97275 Myod1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273611 – 318Myoblast determination protein 1Add BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei104N6-methyllysine; by EHMT2By similarity1

Post-translational modificationi

Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.1 Publication
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.By similarity
Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation (By similarity).By similarity
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP10085
PRIDEiP10085

PTM databases

iPTMnetiP10085
PhosphoSitePlusiP10085

Expressioni

Gene expression databases

BgeeiENSMUSG00000009471
CleanExiMM_MYOD1
GenevisibleiP10085 MM

Interactioni

Subunit structurei

Interacts with SUV39H1 (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 isoform 1 and isoform 4. Interacts with SETD3 (PubMed:21832073). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (PubMed:12037670). Interacts with CSRP3 (By similarity).By similarity5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • nuclear hormone receptor binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • transcription factor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: MGI

Protein-protein interaction databases

BioGridi201673, 30 interactors
CORUMiP10085
DIPiDIP-37N
ELMiP10085
IntActiP10085, 18 interactors
MINTiP10085
STRINGi10090.ENSMUSP00000072330

Structurei

Secondary structure

1318
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi106 – 133Combined sources28
Helixi146 – 162Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MDYX-ray2.80A102-166[»]
B/C/D105-166[»]
ProteinModelPortaliP10085
SMRiP10085
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10085

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 160bHLHPROSITE-ProRule annotationAdd BLAST52

Phylogenomic databases

eggNOGiKOG3960 Eukaryota
ENOG4111SED LUCA
GeneTreeiENSGT00530000063004
HOGENOMiHOG000234800
HOVERGENiHBG006429
InParanoidiP10085
KOiK09064
OMAiPNPIYQV
OrthoDBiEOG091G0GKD
TreeFamiTF316344

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR002546 Basic
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR022032 Myf5
PfamiView protein in Pfam
PF01586 Basic, 1 hit
PF00010 HLH, 1 hit
PF12232 Myf5, 1 hit
SMARTiView protein in SMART
SM00520 BASIC, 1 hit
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

P10085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLSPPLRD IDLTGPDGSL CSFETADDFY DDPCFDSPDL RFFEDLDPRL
60 70 80 90 100
VHMGALLKPE EHAHFPTAVH PGPGAREDEH VRAPSGHHQA GRCLLWACKA
110 120 130 140 150
CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL
160 170 180 190 200
RNAIRYIEGL QALLRDQDAA PPGAAAFYAP GPLPPGRGSE HYSGDSDASS
210 220 230 240 250
PRSNCSDGMM DYSGPPSGPR RQNGYDTAYY SEAARESRPG KSAAVSSLDC
260 270 280 290 300
LSSIVERIST DSPAAPALLL ADAPPESPPG PPEGASLSDT EQGTQTPSPD
310
AAPQCPAGSN PNAIYQVL
Length:318
Mass (Da):34,233
Last modified:July 27, 2011 - v2
Checksum:iAEC6572277A78D54
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53M → V in AAA39799 (PubMed:3690668).Curated1
Sequence conflicti53M → V in CAA43836 (PubMed:1754380).Curated1
Sequence conflicti53M → V in AAA39798 (PubMed:3286015).Curated1
Sequence conflicti66P → S in AAA39799 (PubMed:3690668).Curated1
Sequence conflicti66P → S in CAA43836 (PubMed:1754380).Curated1
Sequence conflicti66P → S in AAA39798 (PubMed:3286015).Curated1
Sequence conflicti234A → V in AAA39799 (PubMed:3690668).Curated1
Sequence conflicti234A → V in CAA43836 (PubMed:1754380).Curated1
Sequence conflicti234A → V in AAA39798 (PubMed:3286015).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18779 mRNA Translation: AAA39799.1
X61655 Genomic DNA Translation: CAA43836.1
M84918 mRNA Translation: AAA39798.1
AK076157 mRNA Translation: BAC36224.1
AK142859 mRNA Translation: BAE25213.1
BC103613 mRNA Translation: AAI03614.1
BC103618 mRNA Translation: AAI03619.1
BC103619 mRNA Translation: AAI03620.1
CCDSiCCDS21277.1
PIRiA29636
RefSeqiNP_034996.2, NM_010866.2
UniGeneiMm.1526

Genome annotation databases

EnsembliENSMUST00000072514; ENSMUSP00000072330; ENSMUSG00000009471
GeneIDi17927
KEGGimmu:17927
UCSCiuc012fkv.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMYOD1_MOUSE
AccessioniPrimary (citable) accession number: P10085
Secondary accession number(s): Q8C6B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 183 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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