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P10081

- IF4A_YEAST

UniProt

P10081 - IF4A_YEAST

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Protein
ATP-dependent RNA helicase eIF4A
Gene
TIF1, TIF41A, YKR059W
TIF2, TIF41B, YJL138C, J0660
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.6 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Kineticsi

  1. KM=470 µM for ATP2 Publications

Vmax=2.6 pmol/sec/µg enzyme for ATP

pH dependencei

Optimum pH is 6.0.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent RNA helicase activity Source: SGD
  3. RNA-dependent ATPase activity Source: SGD
  4. protein binding Source: IntAct
  5. translation initiation factor activity Source: SGD

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. regulation of translational initiation Source: SGD
  3. translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31584-MONOMER.
YEAST:G3O-32027-MONOMER.
SABIO-RKP10081.

Names & Taxonomyi

Organism-specific databases

Protein namesi
Recommended name:
ATP-dependent RNA helicase eIF4A (EC:3.6.4.13)
Alternative name(s):
Eukaryotic initiation factor 4A
Short name:
eIF-4A
Stimulator factor I 37 kDa component
Translation initiation factor 1/2
p37
Gene namesi
Name:TIF1
Synonyms:TIF41A
Ordered Locus Names:YKR059W
AND
Name:TIF2
Synonyms:TIF41B
Ordered Locus Names:YJL138C
ORF Names:J0660
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X, UP000002311: Chromosome XI
SGDiS000001767. TIF1.
S000003674. TIF2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. eukaryotic translation initiation factor 4F complex Source: SGD
  3. ribosome Source: SGD

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241F → A: Lethal in vivo and reduces ATP binding and ATPase activity in vitro. 1 Publication
Mutagenesisi42 – 421F → A: Slow growth in vivo and reduces ATP binding and ATPase activity in vitro. 1 Publication
Mutagenesisi46 – 461S → A: Reduces ATP binding and ATPase activity in vitro. 1 Publication
Mutagenesisi49 – 491Q → A: Reduces ATP binding and ATPase activity in vitro. 1 Publication
Mutagenesisi49 – 491Q → E: Increases about 3-fold ATP binding but reduces about 2-fold ATPase activity in vitro. 1 Publication
Mutagenesisi66 – 661A → D: Slow growth. 2 Publications
Mutagenesisi66 – 661A → V: Lethal and dominant negative if overexpressed in vivo. Impairs ATP hydrolysis, RNA-helicase and translation activity in vitro. 2 Publications
Mutagenesisi72 – 721K → A: Lethal in vivo and reduces ATP binding and ATPase activity in vitro. 1 Publication
Mutagenesisi79 – 791A → V in TIF1-1; no growth at 36 degrees Celsius. 1 Publication
Mutagenesisi126 – 1261G → D: Lethal. 1 Publication
Mutagenesisi127 – 1271G → D: Slow growth. 1 Publication
Mutagenesisi145 – 1451G → D: Lethal. 1 Publication
Mutagenesisi145 – 1451G → S: Slow growth. 1 Publication
Mutagenesisi170 – 1701D → E: Lethal. 1 Publication
Mutagenesisi173 – 1731D → H: Lethal. 1 Publication
Mutagenesisi311 – 3111S → F: Slow growth. 1 Publication
Mutagenesisi347 – 3471R → E, G, I, S or T: Lethal and dominant negative if overexpressed. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 395394ATP-dependent RNA helicase eIF4A
PRO_0000054968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei73 – 731Phosphothreonine1 Publication
Modified residuei77 – 771Phosphoserine1 Publication
Modified residuei129 – 1291Phosphoserine1 Publication
Modified residuei146 – 1461Phosphothreonine3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP10081.
PaxDbiP10081.

2D gel databases

SWISS-2DPAGEP10081.

Expressioni

Gene expression databases

GenevestigatoriP10081.

Interactioni

Subunit structurei

Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A (TIF1/TIF2), eIF4E (TIF45) and eIF4G (TIF4631 or TIF4632) By similarity. Interacts with eIF4G1/TIF4631 and eIF4G2/TIF4632.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIF4631P399357EBI-9017,EBI-9002

Protein-protein interaction databases

BioGridi33619. 156 interactions.
34190. 123 interactions.
DIPiDIP-4571N.
IntActiP10081. 51 interactions.
MINTiMINT-8285693.
STRINGi4932.YKR059W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 175
Helixi24 – 274
Helixi31 – 4010
Helixi47 – 5711
Beta strandi62 – 654
Helixi72 – 8312
Beta strandi93 – 964
Helixi100 – 11314
Turni114 – 1163
Beta strandi121 – 1244
Helixi130 – 13910
Beta strandi141 – 1455
Helixi147 – 1559
Beta strandi166 – 1705
Helixi172 – 1776
Helixi181 – 19010
Beta strandi196 – 2038
Helixi206 – 21510
Beta strandi220 – 2223
Beta strandi235 – 2439
Helixi244 – 2463
Helixi247 – 25711
Beta strandi263 – 2697
Helixi270 – 28213
Beta strandi287 – 2904
Helixi296 – 30712
Beta strandi312 – 3176
Helixi318 – 3203
Turni321 – 3233
Beta strandi330 – 3367
Helixi341 – 3466
Beta strandi359 – 3657
Turni366 – 3683
Helixi369 – 37810
Turni391 – 3944

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FUKX-ray1.75A231-395[»]
1FUUX-ray2.50A/B2-395[»]
1QDEX-ray2.00A9-232[»]
1QVAX-ray2.50A2-224[»]
2VSOX-ray2.60A/B1-395[»]
2VSXX-ray2.80A/B1-395[»]
ProteinModelPortaliP10081.
SMRiP10081. Positions 12-395.

Miscellaneous databases

EvolutionaryTraceiP10081.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 222170Helicase ATP-binding
Add
BLAST
Domaini233 – 394162Helicase C-terminal
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi22 – 5029Q motif
Add
BLAST
Motifi170 – 1734DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00740000115544.
HOGENOMiHOG000268797.
KOiK03257.
OMAiVMNGNQI.
OrthoDBiEOG7FNCJ0.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10081-1 [UniParc]FASTAAdd to Basket

« Hide

MSEGITDIEE SQIQTNYDKV VYKFDDMELD ENLLRGVFGY GFEEPSAIQQ    50
RAIMPIIEGH DVLAQAQSGT GKTGTFSIAA LQRIDTSVKA PQALMLAPTR 100
ELALQIQKVV MALAFHMDIK VHACIGGTSF VEDAEGLRDA QIVVGTPGRV 150
FDNIQRRRFR TDKIKMFILD EADEMLSSGF KEQIYQIFTL LPPTTQVVLL 200
SATMPNDVLE VTTKFMRNPV RILVKKDELT LEGIKQFYVN VEEEEYKYEC 250
LTDLYDSISV TQAVIFCNTR RKVEELTTKL RNDKFTVSAI YSDLPQQERD 300
TIMKEFRSGS SRILISTDLL ARGIDVQQVS LVINYDLPAN KENYIHRIGR 350
GGRFGRKGVA INFVTNEDVG AMRELEKFYS TQIEELPSDI ATLLN 395
Length:395
Mass (Da):44,697
Last modified:January 23, 2007 - v3
Checksum:i19D8C133C815DD48
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12813 Genomic DNA. Translation: CAA31301.1.
X12814 Genomic DNA. Translation: CAA31302.1.
X58099 Genomic DNA. Translation: CAA41110.1.
X87371 Genomic DNA. Translation: CAA60817.1.
Z49413 Genomic DNA. Translation: CAA89433.1.
U25436 Genomic DNA. Translation: AAA91645.1.
Z28284 Genomic DNA. Translation: CAA82138.1.
BK006943 Genomic DNA. Translation: DAA08662.1.
BK006944 Genomic DNA. Translation: DAA09210.1.
PIRiS05835. FIBY1.
RefSeqiNP_012397.1. NM_001181571.1.
NP_012985.3. NM_001179849.3.

Genome annotation databases

EnsemblFungiiYJL138C; YJL138C; YJL138C.
YKR059W; YKR059W; YKR059W.
GeneIDi853303.
853933.
KEGGisce:YJL138C.
sce:YKR059W.

Cross-referencesi

Web resourcesi

RNA helicases from the baker's yeast Saccharomyces cerevisiae

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12813 Genomic DNA. Translation: CAA31301.1 .
X12814 Genomic DNA. Translation: CAA31302.1 .
X58099 Genomic DNA. Translation: CAA41110.1 .
X87371 Genomic DNA. Translation: CAA60817.1 .
Z49413 Genomic DNA. Translation: CAA89433.1 .
U25436 Genomic DNA. Translation: AAA91645.1 .
Z28284 Genomic DNA. Translation: CAA82138.1 .
BK006943 Genomic DNA. Translation: DAA08662.1 .
BK006944 Genomic DNA. Translation: DAA09210.1 .
PIRi S05835. FIBY1.
RefSeqi NP_012397.1. NM_001181571.1.
NP_012985.3. NM_001179849.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FUK X-ray 1.75 A 231-395 [» ]
1FUU X-ray 2.50 A/B 2-395 [» ]
1QDE X-ray 2.00 A 9-232 [» ]
1QVA X-ray 2.50 A 2-224 [» ]
2VSO X-ray 2.60 A/B 1-395 [» ]
2VSX X-ray 2.80 A/B 1-395 [» ]
ProteinModelPortali P10081.
SMRi P10081. Positions 12-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33619. 156 interactions.
34190. 123 interactions.
DIPi DIP-4571N.
IntActi P10081. 51 interactions.
MINTi MINT-8285693.
STRINGi 4932.YKR059W.

2D gel databases

SWISS-2DPAGE P10081.

Proteomic databases

MaxQBi P10081.
PaxDbi P10081.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL138C ; YJL138C ; YJL138C .
YKR059W ; YKR059W ; YKR059W .
GeneIDi 853303.
853933.
KEGGi sce:YJL138C.
sce:YKR059W.

Organism-specific databases

SGDi S000001767. TIF1.
S000003674. TIF2.

Phylogenomic databases

eggNOGi COG0513.
GeneTreei ENSGT00740000115544.
HOGENOMi HOG000268797.
KOi K03257.
OMAi VMNGNQI.
OrthoDBi EOG7FNCJ0.

Enzyme and pathway databases

BioCyci YEAST:G3O-31584-MONOMER.
YEAST:G3O-32027-MONOMER.
SABIO-RK P10081.

Miscellaneous databases

EvolutionaryTracei P10081.
NextBioi 973627.

Gene expression databases

Genevestigatori P10081.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the genes TIF1 and TIF2 from Saccharomyces cerevisiae coding for a translation initiation factor."
    Linder P., Slonimski P.P.
    Nucleic Acids Res. 16:10359-10359(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (TIF1 AND TIF2).
    Strain: CD11/B1830/50.
  2. "An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation."
    Linder P., Slonimski P.P.
    Proc. Natl. Acad. Sci. U.S.A. 86:2286-2290(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (TIF1 AND TIF2).
    Strain: CD11/B1830/50.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (TIF1).
    Strain: ATCC 204508 / S288c.
  4. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
    Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
    Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (TIF2).
    Strain: ATCC 96604 / S288c / FY1679.
  5. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (TIF2).
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION (TIF1 AND TIF2).
    Strain: ATCC 204508 / S288c.
  7. "Requirement of the self-glucosylating initiator proteins Glg1p and Glg2p for glycogen accumulation in Saccharomyces cerevisiae."
    Cheng C., Mu J., Farkas I., Huang D., Goebl M.G., Roach P.J.
    Mol. Cell. Biol. 15:6632-6640(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93 (TIF2).
  8. "The Saccharomyces cerevisiae RPB4 gene is tightly linked to the TIF2 gene."
    Foreman P.K., Davis R.W., Sachs A.B.
    Nucleic Acids Res. 19:2781-2781(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 307-395 (TIF2).
  9. "The 66 kDa component of yeast SFI, stimulatory factor I, is hsp60."
    Smiley J.K., Brown W.C., Campbell J.L.
    Nucleic Acids Res. 20:4913-4918(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 332-340; 342-347 AND 378-391.
  10. "Translation in Saccharomyces cerevisiae: initiation factor 4A-dependent cell-free system."
    Blum S., Mueller M., Schmid S.R., Linder P., Trachsel H.
    Proc. Natl. Acad. Sci. U.S.A. 86:6043-6046(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Translation initiation factor-dependent extracts from Saccharomyces cerevisiae."
    Altmann M., Blum S., Pelletier J., Sonenberg N., Wilson T.M., Trachsel H.
    Biochim. Biophys. Acta 1050:155-159(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Translation initiation factor 4A from Saccharomyces cerevisiae: analysis of residues conserved in the D-E-A-D family of RNA helicases."
    Schmid S.R., Linder P.
    Mol. Cell. Biol. 11:3463-3471(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF ALA-66; ALA-79; GLY-126; GLY-127; GLY-145; ASP-170; ASP-173; SER-311 AND ARG-347.
  13. "ATP hydrolysis by initiation factor 4A is required for translation initiation in Saccharomyces cerevisiae."
    Blum S., Schmid S.R., Pause A., Buser P., Linder P., Sonenberg N., Trachsel H.
    Proc. Natl. Acad. Sci. U.S.A. 89:7664-7668(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ALA-66.
  14. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
    Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
    Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2.
  15. "Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally."
    Neff C.L., Sachs A.B.
    Mol. Cell. Biol. 19:5557-5564(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TIF4631 AND TIF4632.
  16. "Interaction of translation initiation factor eIF4G with eIF4A in the yeast Saccharomyces cerevisiae."
    Dominguez D., Altmann M., Benz J., Baumann U., Trachsel H.
    J. Biol. Chem. 274:26720-26726(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TIF4631.
  17. "The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis."
    Tanner N.K., Cordin O., Banroques J., Doere M., Linder P.
    Mol. Cell 11:127-138(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF PHE-24; PHE-42; SER-46; GLN-49 AND LYS-72.
  18. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  19. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  20. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  21. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129 AND THR-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  22. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-73; SER-77 AND THR-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  23. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  24. "Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase."
    Johnson E.R., McKay D.B.
    RNA 5:1526-1534(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 3-225.
  25. "Crystal structure of the ATPase domain of translation initiation factor 4A from Saccharomyces cerevisiae -- the prototype of the DEAD box protein family."
    Benz J., Trachsel H., Baumann U.
    Structure 7:671-679(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 10-233.
  26. "Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase."
    Caruthers J.M., Johnson E.R., McKay D.B.
    Proc. Natl. Acad. Sci. U.S.A. 97:13080-13085(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 231-395.

Entry informationi

Entry nameiIF4A_YEAST
AccessioniPrimary (citable) accession number: P10081
Secondary accession number(s): D6VW46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 173 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

TIF1 and TIF2 code for the same protein.
Present with 106000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
  6. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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