P10080 (SSBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Single-stranded nucleic acid-binding protein

Gene names

Name:	SBP1
Synonyms:	SSB1, SSBR1
Ordered Locus Names:	YHL034C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in the transition of mRNAs from translation to an mRNP complex destined for decapping. High-copy-number suppressor of decapping defects. Overexpression suppresses decapping defects in both DCP1-2 and DCP2-7 mutations. Acts to promote translational repression of mRNA in conjunction with DHH1 and subsequent mRNA localization to P bodies. Promotes translational repression of mRNA during glucose deprivation. Ref.8
Subunit structure	Associated with snR10 and snR11 small nuclear RNAs.
Subcellular location	Cytoplasm. Nucleus › nucleolus. Note: Found in cytoplasmic P bodies. Ref.5 Ref.6 Ref.8
Miscellaneous	Present with 12800 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the RRM GAR family. Contains 2 RRM (RNA recognition motif) domains.
Sequence caution	The sequence AAA35098.1 differs from that shown. Reason: Frameshift at positions 162, 175 and 181.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of translation in response to stress Inferred from mutant phenotype Ref.8. Source: SGD
Cellular component	cytoplasmic mRNA processing body Inferred from direct assay Ref.8. Source: SGD nucleolus Inferred from direct assay Ref.5Ref.1. Source: SGD
Molecular function	RNA binding Non-traceable author statement Ref.1. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Single-stranded nucleic acid-binding protein		PRO_0000081964
Regions
	Domain	37 – 119	83	RRM 1
	Domain	186 – 274	89	RRM 2
	Region	131 – 151	21	RNA-binding RGG-box By similarity
Amino acid modifications
	Modified residue	91	1	Phosphothreonine Ref.9 Ref.11 Ref.12
	Modified residue	119	1	Phosphothreonine Ref.12
	Modified residue	230	1	Phosphothreonine Ref.12
	Modified residue	231	1	Phosphoserine Ref.10
	Modified residue	233	1	Phosphoserine Ref.12
	Modified residue	242	1	Phosphothreonine Ref.10
	Modified residue	244	1	Phosphoserine Ref.10
	Modified residue	289	1	Phosphoserine Ref.12
Experimental info
	Sequence conflict	162	1	G → S in AAA35098. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10080 [UniParc]. Last modified October 1, 1993. Version 2. Checksum: FF740B206DDE5EC8	FASTA	294	32,990
	10 20 30 40 50 60 MSAEIEEATN AVNNLSINDS EQQPRAPTHK TVIDPEDTIF IGNVAHECTE DDLKQLFVEE 70 80 90 100 110 120 FGDEVSVEIP IKEHTDGHIP ASKHALVKFP TKIDFDNIKE NYDTKVVKDR EIHIKRARTP 130 140 150 160 170 180 GQMQRGGFRG RGGFRGRGGF RGGFRGGYRG GFRGRGNFRG RGGARGGFNG QKREKIPLDQ 190 200 210 220 230 240 MERSKDTLYI NNVPFKATKE EVAEFFGTDA DSISLPMRKM RDQHTGRIFT SDSANRGMAF 250 260 270 280 290 VTFSGENVDI EAKAEEFKGK VFGDRELTVD VAVIRPENDE EEIEQETGSE EKQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae SSB1 protein and its relationship to nucleolar RNA-binding proteins." Jong A.Y.-S., Clark M.W., Gilbert M., Oehm A., Campbell J.L. Mol. Cell. Biol. 7:2947-2955(1987) [PubMed: 2823109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"NOP3 is an essential yeast protein which is required for pre-rRNA processing." Russell I.D., Tollervey D. J. Cell Biol. 119:737-747(1992) [PubMed: 1429834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-294.
[5]	"SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs." Clark M.W., Yip M.L.R., Campbell J., Abelson J. J. Cell Biol. 111:1741-1751(1990) [PubMed: 2121740] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Sbp1p affects translational repression and decapping in Saccharomyces cerevisiae." Segal S.P., Dunckley T., Parker R. Mol. Cell. Biol. 26:5120-5130(2006) [PubMed: 16782896] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; THR-242 AND SER-244, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91; THR-119; THR-230; SER-233 AND SER-289, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17244 Genomic DNA. Translation: AAA35098.1. Frameshift. U11583 Genomic DNA. Translation: AAB65046.1. X66278 Genomic DNA. Translation: CAA46989.1. BK006934 Genomic DNA. Translation: DAA06651.1.
PIR	S48934.
RefSeq	NP_011829.1. NM_001179114.1.
3D structure databases
ProteinModelPortal	P10080.
SMR	P10080. Positions 31-121, 186-272.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6824N.
IntAct	P10080. 78 interactions.
MINT	MINT-667006.
STRING	P10080.
Proteomic databases
PeptideAtlas	P10080.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL034C; YHL034C; YHL034C.
GeneID	856351.
KEGG	sce:YHL034C.
NMPDR	fig|4932.3.peg.2966.
Organism-specific databases
CYGD	YHL034c.
SGD	S000001026. SBP1.
Phylogenomic databases
eggNOG	fuNOG10715.
GeneTree	EFGT00050000000575.
HOGENOM	HBG203771.
OMA	EDTIFIG.
OrthoDB	EOG4S4SRP.
Gene expression databases
ArrayExpress	P10080.
Genevestigator	P10080.
GermOnline	YHL034C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	981790.

Entry information

Entry name

SSBP1_YEAST

Accession

Primary (citable) accession number: P10080
Secondary accession number(s): D3DKT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program