Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Caricain

Gene
N/A
Organism
Carica papaya (Papaya)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, similar to those of papain and chymopapain.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1571
Active sitei2911
Active sitei3111

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.30. 1191.

Protein family/group databases

MEROPSiI29.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Caricain (EC:3.4.22.30)
Alternative name(s):
Papaya peptidase A
Papaya proteinase III
Short name:
PPIII
Papaya proteinase omega
OrganismiCarica papaya (Papaya)
Taxonomic identifieri3649 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCaricaceaeCarica

Pathology & Biotechi

Protein family/group databases

Allergomei1539. Car p Endoproteinase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000002641017 – 132Activation peptide1 PublicationAdd BLAST116
ChainiPRO_0000026411133 – 348CaricainAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi154 ↔ 195
Disulfide bondi188 ↔ 227
Disulfide bondi285 ↔ 336

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 53Combined sources14
Turni54 – 56Combined sources3
Helixi62 – 83Combined sources22
Beta strandi88 – 91Combined sources4
Turni95 – 98Combined sources4
Helixi101 – 108Combined sources8
Beta strandi122 – 124Combined sources3
Turni139 – 143Combined sources5
Beta strandi153 – 155Combined sources3
Helixi157 – 174Combined sources18
Helixi182 – 188Combined sources7
Beta strandi190 – 192Combined sources3
Helixi194 – 196Combined sources3
Helixi200 – 210Combined sources11
Beta strandi212 – 214Combined sources3
Turni215 – 217Combined sources3
Helixi229 – 231Combined sources3
Beta strandi241 – 244Combined sources4
Beta strandi247 – 249Combined sources3
Helixi250 – 259Combined sources10
Beta strandi262 – 266Combined sources5
Helixi271 – 275Combined sources5
Beta strandi278 – 281Combined sources4
Beta strandi291 – 301Combined sources11
Beta strandi304 – 310Combined sources7
Beta strandi315 – 317Combined sources3
Beta strandi322 – 326Combined sources5
Beta strandi330 – 333Combined sources4
Helixi335 – 337Combined sources3
Beta strandi343 – 346Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C7Xmodel-A133-348[»]
1EUXmodel-A133-348[»]
1F1Imodel-A133-348[»]
1MEGX-ray2.00A133-348[»]
1PCIX-ray3.20A/B/C27-348[»]
1PPOX-ray1.80A133-348[»]
ProteinModelPortaliP10056.
SMRiP10056.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10056.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20739.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMIPSISKL LFVAICLFVH MSVSFGDFSI VGYSQDDLTS TERLIQLFNS
60 70 80 90 100
WMLNHNKFYE NVDEKLYRFE IFKDNLNYID ETNKKNNSYW LGLNEFADLS
110 120 130 140 150
NDEFNEKYVG SLIDATIEQS YDEEFINEDT VNLPENVDWR KKGAVTPVRH
160 170 180 190 200
QGSCGSCWAF SAVATVEGIN KIRTGKLVEL SEQELVDCER RSHGCKGGYP
210 220 230 240 250
PYALEYVAKN GIHLRSKYPY KAKQGTCRAK QVGGPIVKTS GVGRVQPNNE
260 270 280 290 300
GNLLNAIAKQ PVSVVVESKG RPFQLYKGGI FEGPCGTKVD HAVTAVGYGK
310 320 330 340
SGGKGYILIK NSWGTAWGEK GYIRIKRAPG NSPGVCGLYK SSYYPTKN
Length:348
Mass (Da):38,788
Last modified:October 1, 1996 - v2
Checksum:i328F5C6BF45B4A07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66060 mRNA. Translation: CAA46862.1.
X51899 mRNA. Translation: CAA36180.1.
PIRiJN0633.

Genome annotation databases

KEGGiag:CAA46862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66060 mRNA. Translation: CAA46862.1.
X51899 mRNA. Translation: CAA36180.1.
PIRiJN0633.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C7Xmodel-A133-348[»]
1EUXmodel-A133-348[»]
1F1Imodel-A133-348[»]
1MEGX-ray2.00A133-348[»]
1PCIX-ray3.20A/B/C27-348[»]
1PPOX-ray1.80A133-348[»]
ProteinModelPortaliP10056.
SMRiP10056.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei1539. Car p Endoproteinase.
MEROPSiI29.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA46862.

Phylogenomic databases

KOiK20739.

Enzyme and pathway databases

BRENDAi3.4.22.30. 1191.

Miscellaneous databases

EvolutionaryTraceiP10056.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAPA3_CARPA
AccessioniPrimary (citable) accession number: P10056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.