P10052 (KITH_FOWPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
Ordered Locus Names:	FPV086

Organism

Fowlpox virus (strain NVSL) (FPV) [Reference proteome]

Taxonomic identifier

928301 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Avipoxvirus › Fowlpox virus

Virus host

Vertebrata [TaxID: 7742]

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Thymidine kinase		PRO_0000174930
Regions
	Nucleotide binding	11 – 18	8	ATP By similarity
	Region	163 – 167	5	Substrate binding By similarity
Sites
	Active site	89	1	Proton acceptor Potential
	Metal binding	144	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	176	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Binding site	119	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10052 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: FA60C5629F2DF276	FASTA	183	20,381
	10 20 30 40 50 60 MSSGSIHVIT GPMFSGKTSE LVRRIKRFML SNFKCIIIKH CGDNRYNEDD INKVYTHDLL 70 80 90 100 110 120 FMEATASSNL SVLVPTLLND GVQVIGIDEA QFFLDIVEFS ESMANLGKTV IVAALNGDFK 130 140 150 160 170 180 RELFGNVYKL LSLAETVSSL TAICVKCYCD ASFSKRVTEN KEVMDIGGKD KYIAVCRKCF FSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fowlpox virus thymidine kinase: nucleotide sequence and relationships to other thymidine kinases." Boyle D.B., Coupar B.E.H., Gibbs A.J., Seigman L.J., Both G.W. Virology 156:355-365(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparison of a conserved region in fowlpox virus and vaccinia virus genomes and the translocation of the fowlpox virus thymidine kinase gene." Binns M.M., Tomley F.M., Campbell J., Boursnell M.E.G. J. Gen. Virol. 69:1275-1283(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FP-9 / Isolate HP-440.
[3]	"Gene translocations in poxviruses: the fowlpox virus thymidine kinase gene is flanked by 15 bp direct repeats and occupies the locus which in vaccinia virus is occupied by the ribonucleotide reductase large subunit gene." Binns M.M., Boursnell M.E.G., Skinner M.A. Virus Res. 24:161-172(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FP-9 / Isolate HP-440.
[4]	Beisel C.E., Nazerian K. Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Vaccine.
[5]	"Sequence of thymidine kinase gene of Indian isolate of fowlpox virus." Senthilvelan A., Purushothaman V., Palaniswami K. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The genome of fowlpox virus." Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L. J. Virol. 74:3815-3831(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16617 Genomic DNA. Translation: AAA43822.1. D00321 Genomic DNA. Translation: BAA00233.1. AJ223385 Genomic DNA. Translation: CAA11295.1. X52860 Genomic DNA. Translation: CAA37041.1. AF198100 Genomic DNA. Translation: AAF44430.1. AF396867 Genomic DNA. Translation: AAK77606.1.
PIR	KIVZFP. A27532.
RefSeq	NP_039049.1. NC_002188.1.
3D structure databases
ProteinModelPortal	P10052.
SMR	P10052. Positions 3-181.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486634.
Phylogenomic databases
ProtClustDB	CLSP2509781.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_FOWPN

Accession

Primary (citable) accession number: P10052

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program