Reviewed, UniProtKB/Swiss-Prot P10047 (DCTB_RHILE)

Names and origin

Protein names

Recommended name:
    C4-dicarboxylate transport sensor protein dctB
    EC=2.7.13.3

Gene names

Name:

dctB

Organism

Rhizobium leguminosarum

Taxonomic identifier

384 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	C4-dicarboxylate transport sensor protein dctB		PRO_0000074747
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 42	18	Potential
	Topological domain	43 – 320	278	Periplasmic
	Transmembrane	321 – 338	18	Potential
	Topological domain	339 – 622	284	Cytoplasmic Potential
	Domain	412 – 622	211	Histidine kinase
Amino acid modifications
	Modified residue	415	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10047-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8FF3B97C2235058F	FASTA	622	67,682
	10 20 30 40 50 60 MHKSAMSVSQ KLWPSLPLQH RIRRMWWTYA ALAFLAVVAS LWTSGEIGQH RAEAALEEQA 70 80 90 100 110 120 RMDVTLNAAL LRTVLEKYRA LPFVLSQDTA LAAALVGNDA GTFERLSQKL EILAAGTKAA 130 140 150 160 170 180 VIYVIDKDGI AVSASNWREP TSFVGNDYRF REYFQGAVER GQAEHFALGT VSKKPGLYIS 190 200 210 220 230 240 QRISGSNGLL GVVVVKVEFD DVEADWNASG TPSYVVDERG IVLITSLPSW RFMTIGRIAE 250 260 270 280 290 300 DRLTAIRESL QFGAAPLQPL PLDMVRNLGE GLDVVEIVMP GDAGKTRFLD VATSVPATGW 310 320 330 340 350 360 HLQHLVALGP SVDAGIREAR MLALLILLPL LAGAAFLLRR RHTIALRISS EQQAREELER 370 380 390 400 410 420 RVVERTLDLS QARDRLQAEI IGHKSTEQKL QAVQQDLVQA NRLAILGQVA AGVAHEINQP 430 440 450 460 470 480 VATIRAYADN ARTFLDRGQT APAGENLESI AALTERIGSI TEELKTFARK GRGSAEPTGL 490 500 510 520 530 540 KDVIEGAVML LRSRFAGRMD TLDIDLPPDE LQVMGNRIRL EQVLINLLQN ALEAVAPKAG 550 560 570 580 590 600 EGRVEIRTST DAGMVTVTVA DNGPGIPTEI RKGLFTPFNT SKESGLGLGL VISKDIVGDY 610 620 GGRMDVASDS GGTRFIVQLR KA

References

[1]

"Deduced products of C4-dicarboxylate transport regulatory genes of Rhizobium leguminosarum are homologous to nitrogen regulatory gene products." Ronson C.W., Astwood P.M., Nixon B.T., Ausubel F.M. Nucleic Acids Res. 15:7921-7934(1987) [PubMed: 3671068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06253 Genomic DNA. Translation: CAA29595.1. Z11529 Genomic DNA. Translation: CAA77619.1.
PIR	A26981.
3D structure databases
SMR	P10047. Positions 401-621.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.13.3. 2202.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR017055. Sig_transdc_His_kinase_DctB. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PIRSF	PIRSF036431. STHK_DctB. 1 hit.
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCTB_RHILE

Accession

Primary (citable) accession number: P10047

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	January 19, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)