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Protein

Nitrilase, bromoxynil-specific

Gene

bxn

Organism
Klebsiella pneumoniae subsp. ozaenae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specific for the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile); converts it to its metabolite 3,5-dibromo-4-hydroxybenzoic acid.

Miscellaneous

Introduced by genetic manipulation and expressed in bromoxynil-tolerant cotton by Monsanto (Calgene).

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Proton acceptorPROSITE-ProRule annotation1
Active sitei127Proton donorPROSITE-ProRule annotation1
Active sitei161NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHerbicide resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase, bromoxynil-specific (EC:3.5.5.1)
Gene namesi
Name:bxn
Encoded oniPlasmid0 Publication
OrganismiKlebsiella pneumoniae subsp. ozaenae
Taxonomic identifieri574 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002040441 – 349Nitrilase, bromoxynil-specificAdd BLAST349

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP10045.
SMRiP10045.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 274CN hydrolasePROSITE-ProRule annotationAdd BLAST270

Sequence similaritiesi

Phylogenomic databases

KOiK20806.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P10045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTTFKAAAV QAEPVWMDAA ATADKTVTLV AKAAAAGAQL VAFPELWIPG
60 70 80 90 100
YPGFMLTHNQ TETLPFIIKY RKQAIAADGP EIEKIRCAAQ EHNIALSFGY
110 120 130 140 150
SERAGRTLYM SQMLIDADGI TKIRRRKLKP TRFERELFGE GDGSDLQVAQ
160 170 180 190 200
TSVGRVGALN CAENLQSLNK FALAAEGEQI HISAWPFTLG SPVLVGDSIG
210 220 230 240 250
AINQVYAAET GTFVLMSTQV VGPTGIAAFE IEDRYNPNQY LGGGYARIYG
260 270 280 290 300
PDMQLKSKSL SPTEEGIVYA EIDLSMLEAA KYSLDPTGHY SRPDVFSVSI
310 320 330 340
NRQRQPAVSE VIDSNGDEDP RAACEPDEGD REVVISTAIG VLPRYCGHS
Length:349
Mass (Da):37,802
Last modified:July 1, 1989 - v1
Checksum:i490B9A8CFD98E732
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03196 Genomic DNA. Translation: AAA25057.1.
PIRiA28658.

Genome annotation databases

KEGGiag:AAA25057.

Similar proteinsi

Entry informationi

Entry nameiNRLB_KLEPO
AccessioniPrimary (citable) accession number: P10045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 25, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Genetically modified food, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families