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P10037

- PIT1_RAT

UniProt

P10037 - PIT1_RAT

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Protein
Pituitary-specific positive transcription factor 1
Gene
Pou1f1, Ghf-1, Pit-1, Pit1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi214 – 27360Homeobox
Add
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  2. RNA polymerase II transcription coactivator activity Source: Ensembl
  3. chromatin binding Source: Ensembl
  4. protein binding Source: RGD
  5. sequence-specific DNA binding Source: Ensembl
  6. transcription corepressor activity Source: RGD
Complete GO annotation...

GO - Biological processi

  1. B cell differentiation Source: Ensembl
  2. adenohypophysis development Source: RGD
  3. cell fate specification Source: Ensembl
  4. determination of adult lifespan Source: Ensembl
  5. negative regulation of transcription from RNA polymerase II promoter Source: RGD
  6. nuclear transport Source: RGD
  7. positive regulation of cell proliferation Source: Ensembl
  8. positive regulation of inositol trisphosphate biosynthetic process Source: Ensembl
  9. positive regulation of multicellular organism growth Source: Ensembl
  10. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  11. positive regulation of transcription, DNA-templated Source: UniProtKB
  12. regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
  13. somatotropin secreting cell development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary-specific positive transcription factor 1
Short name:
PIT-1
Alternative name(s):
Growth hormone factor 1
Short name:
GHF-1
Gene namesi
Name:Pou1f1
Synonyms:Ghf-1, Pit-1, Pit1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 11

Organism-specific databases

RGDi3367. Pou1f1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Pituitary-specific positive transcription factor 1
PRO_0000100701Add
BLAST

Proteomic databases

PaxDbiP10037.

PTM databases

PhosphoSiteiP10037.

Expressioni

Gene expression databases

GenevestigatoriP10037.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi129 – 14618
Helixi150 – 15910
Helixi167 – 1748
Helixi180 – 19617
Helixi223 – 23513
Helixi241 – 25111
Helixi255 – 26814

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AU7X-ray2.30A/B128-273[»]
ProteinModelPortaliP10037.
SMRiP10037. Positions 129-273.

Miscellaneous databases

EvolutionaryTraceiP10037.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 19875POU-specific
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG241031.
GeneTreeiENSGT00730000110809.
HOVERGENiHBG053120.
KOiK09363.
OMAiTATGLHY.
OrthoDBiEOG7DJSMG.
PhylomeDBiP10037.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform B (identifier: P10037-1) [UniParc]FASTAAdd to Basket

Also known as: PIT-1B, GHF-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSCQPFTSAD TFIPLNSDAS AALPLRMHHS AAEGLPASNH ATNVMSTATG    50
LHYSVPSCHY GNQPSTYGVM AGTLTPCLYK FPDHTLSHGF PPLHQPLLAE 100
DPTASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ 150
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV 200
GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEHSKPS SQEIMRMAEE 250
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R 291
Length:291
Mass (Da):32,903
Last modified:April 27, 2001 - v3
Checksum:i2651876B2F7668A1
GO
Isoform A (identifier: P10037-2) [UniParc]FASTAAdd to Basket

Also known as: PIT-1A, GHF-2

The sequence of this isoform differs from the canonical sequence as follows:
     47-47: T → TVPSILSLIQTPKCLHTYFSMTTMGNT

Note: Altered in its ability to trans-activate compared to isoform B.

Show »
Length:317
Mass (Da):35,770
Checksum:iF217B4F2307D7940
GO

Sequence cautioni

The sequence AAA41851.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAA41853.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei47 – 471T → TVPSILSLIQTPKCLHTYFS MTTMGNT in isoform A.
VSP_002316

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65364
, X65365, X65367, X65368 Genomic DNA. Translation: CAA46440.1.
L01506 mRNA. Translation: AAA41850.1.
L01506 mRNA. Translation: AAA41851.1. Different initiation.
L01507 mRNA. Translation: AAA41852.1.
L01507 mRNA. Translation: AAA41853.1. Different initiation.
S97398 mRNA. Translation: AAC60656.1.
M23253 mRNA. Translation: AAA41854.1.
X12658 mRNA. Translation: CAA31185.1.
X63089 mRNA. No translation available.
PIRiS22705.
RefSeqiXP_006248036.1. XM_006247974.1. [P10037-2]
UniGeneiRn.10445.

Genome annotation databases

EnsembliENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715. [P10037-2]
GeneIDi25517.
KEGGirno:25517.
UCSCiRGD:3367. rat. [P10037-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65364
, X65365 , X65367 , X65368 Genomic DNA. Translation: CAA46440.1 .
L01506 mRNA. Translation: AAA41850.1 .
L01506 mRNA. Translation: AAA41851.1 . Different initiation.
L01507 mRNA. Translation: AAA41852.1 .
L01507 mRNA. Translation: AAA41853.1 . Different initiation.
S97398 mRNA. Translation: AAC60656.1 .
M23253 mRNA. Translation: AAA41854.1 .
X12658 mRNA. Translation: CAA31185.1 .
X63089 mRNA. No translation available.
PIRi S22705.
RefSeqi XP_006248036.1. XM_006247974.1. [P10037-2 ]
UniGenei Rn.10445.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AU7 X-ray 2.30 A/B 128-273 [» ]
ProteinModelPortali P10037.
SMRi P10037. Positions 129-273.
ModBasei Search...

PTM databases

PhosphoSitei P10037.

Proteomic databases

PaxDbi P10037.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000000905 ; ENSRNOP00000000905 ; ENSRNOG00000000715 . [P10037-2 ]
GeneIDi 25517.
KEGGi rno:25517.
UCSCi RGD:3367. rat. [P10037-1 ]

Organism-specific databases

CTDi 5449.
RGDi 3367. Pou1f1.

Phylogenomic databases

eggNOGi NOG241031.
GeneTreei ENSGT00730000110809.
HOVERGENi HBG053120.
KOi K09363.
OMAi TATGLHY.
OrthoDBi EOG7DJSMG.
PhylomeDBi P10037.
TreeFami TF316413.

Miscellaneous databases

EvolutionaryTracei P10037.
PROi P10037.

Gene expression databases

Genevestigatori P10037.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view ]
PANTHERi PTHR11636:SF82. PTHR11636:SF82. 1 hit.
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein."
    Bodner M., Castrillo J.-L., Theill L.E., Deerinck T., Ellisman M., Karin M.
    Cell 55:505-518(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
  2. "A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype."
    Ingraham H.A., Chen R., Mangalam H.J., Elsholtz H.P., Flynn S.E., Lin C.R., Simmons D.M., Swanson L., Rosenfeld M.G.
    Cell 55:519-529(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
  3. "Differential splicing of the GHF1 primary transcript gives rise to two functionally distinct homeodomain proteins."
    Theill L.E., Hattori K., Lazzaro D., Castrillo J.-L., Karin M.
    EMBO J. 11:2261-2269(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
  4. "Functional isoforms of Pit-1 generated by alternative messenger RNA splicing."
    Konzak K.E., Moore D.D.
    Mol. Endocrinol. 6:241-247(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
  5. "An alternatively spliced Pit-1 isoform altered in its ability to trans-activate."
    Morris A.E., Kloss B., McChesney R.E., Bancroft C., Chasin L.A.
    Nucleic Acids Res. 20:1355-1361(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  6. "Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility."
    Jacobson E.M., Li P., Leon-Del-Rio A., Rosenfeld M.G., Aggarwal A.K.
    Genes Dev. 11:198-212(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 130-273.
    Strain: Sprague-Dawley.
    Tissue: Pituitary.

Entry informationi

Entry nameiPIT1_RAT
AccessioniPrimary (citable) accession number: P10037
Secondary accession number(s): Q63431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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