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P10037

- PIT1_RAT

UniProt

P10037 - PIT1_RAT

Protein

Pituitary-specific positive transcription factor 1

Gene

Pou1f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 153 (01 Oct 2014)
      Sequence version 3 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi214 – 27360HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. protein binding Source: RGD
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    5. RNA polymerase II transcription coactivator activity Source: Ensembl
    6. transcription corepressor activity Source: RGD

    GO - Biological processi

    1. adenohypophysis development Source: RGD
    2. B cell differentiation Source: Ensembl
    3. cell fate specification Source: Ensembl
    4. determination of adult lifespan Source: Ensembl
    5. negative regulation of transcription from RNA polymerase II promoter Source: RGD
    6. nuclear transport Source: RGD
    7. positive regulation of cell proliferation Source: Ensembl
    8. positive regulation of inositol trisphosphate biosynthetic process Source: Ensembl
    9. positive regulation of multicellular organism growth Source: Ensembl
    10. positive regulation of transcription, DNA-templated Source: UniProtKB
    11. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    12. regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
    13. somatotropin secreting cell development Source: Ensembl

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pituitary-specific positive transcription factor 1
    Short name:
    PIT-1
    Alternative name(s):
    Growth hormone factor 1
    Short name:
    GHF-1
    Gene namesi
    Name:Pou1f1
    Synonyms:Ghf-1, Pit-1, Pit1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 11

    Organism-specific databases

    RGDi3367. Pou1f1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: RGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Pituitary-specific positive transcription factor 1PRO_0000100701Add
    BLAST

    Proteomic databases

    PaxDbiP10037.

    PTM databases

    PhosphoSiteiP10037.

    Expressioni

    Gene expression databases

    GenevestigatoriP10037.

    Structurei

    Secondary structure

    1
    291
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi129 – 14618
    Helixi150 – 15910
    Helixi167 – 1748
    Helixi180 – 19617
    Helixi223 – 23513
    Helixi241 – 25111
    Helixi255 – 26814

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AU7X-ray2.30A/B128-273[»]
    ProteinModelPortaliP10037.
    SMRiP10037. Positions 129-273.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10037.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini124 – 19875POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG241031.
    GeneTreeiENSGT00730000110809.
    HOVERGENiHBG053120.
    KOiK09363.
    OMAiTATGLHY.
    OrthoDBiEOG7DJSMG.
    PhylomeDBiP10037.
    TreeFamiTF316413.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR015586. Pit_1.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view]
    PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform B (identifier: P10037-1) [UniParc]FASTAAdd to Basket

    Also known as: PIT-1B, GHF-1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSCQPFTSAD TFIPLNSDAS AALPLRMHHS AAEGLPASNH ATNVMSTATG    50
    LHYSVPSCHY GNQPSTYGVM AGTLTPCLYK FPDHTLSHGF PPLHQPLLAE 100
    DPTASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ 150
    TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV 200
    GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEHSKPS SQEIMRMAEE 250
    LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R 291
    Length:291
    Mass (Da):32,903
    Last modified:April 27, 2001 - v3
    Checksum:i2651876B2F7668A1
    GO
    Isoform A (identifier: P10037-2) [UniParc]FASTAAdd to Basket

    Also known as: PIT-1A, GHF-2

    The sequence of this isoform differs from the canonical sequence as follows:
         47-47: T → TVPSILSLIQTPKCLHTYFSMTTMGNT

    Note: Altered in its ability to trans-activate compared to isoform B.

    Show »
    Length:317
    Mass (Da):35,770
    Checksum:iF217B4F2307D7940
    GO

    Sequence cautioni

    The sequence AAA41851.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAA41853.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei47 – 471T → TVPSILSLIQTPKCLHTYFS MTTMGNT in isoform A. CuratedVSP_002316

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65364
    , X65365, X65367, X65368 Genomic DNA. Translation: CAA46440.1.
    L01506 mRNA. Translation: AAA41850.1.
    L01506 mRNA. Translation: AAA41851.1. Different initiation.
    L01507 mRNA. Translation: AAA41852.1.
    L01507 mRNA. Translation: AAA41853.1. Different initiation.
    S97398 mRNA. Translation: AAC60656.1.
    M23253 mRNA. Translation: AAA41854.1.
    X12658 mRNA. Translation: CAA31185.1.
    X63089 mRNA. No translation available.
    PIRiS22705.
    RefSeqiXP_006248036.1. XM_006247974.1. [P10037-2]
    UniGeneiRn.10445.

    Genome annotation databases

    EnsembliENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715. [P10037-2]
    GeneIDi25517.
    KEGGirno:25517.
    UCSCiRGD:3367. rat. [P10037-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65364
    , X65365 , X65367 , X65368 Genomic DNA. Translation: CAA46440.1 .
    L01506 mRNA. Translation: AAA41850.1 .
    L01506 mRNA. Translation: AAA41851.1 . Different initiation.
    L01507 mRNA. Translation: AAA41852.1 .
    L01507 mRNA. Translation: AAA41853.1 . Different initiation.
    S97398 mRNA. Translation: AAC60656.1 .
    M23253 mRNA. Translation: AAA41854.1 .
    X12658 mRNA. Translation: CAA31185.1 .
    X63089 mRNA. No translation available.
    PIRi S22705.
    RefSeqi XP_006248036.1. XM_006247974.1. [P10037-2 ]
    UniGenei Rn.10445.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AU7 X-ray 2.30 A/B 128-273 [» ]
    ProteinModelPortali P10037.
    SMRi P10037. Positions 129-273.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P10037.

    Proteomic databases

    PaxDbi P10037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000000905 ; ENSRNOP00000000905 ; ENSRNOG00000000715 . [P10037-2 ]
    GeneIDi 25517.
    KEGGi rno:25517.
    UCSCi RGD:3367. rat. [P10037-1 ]

    Organism-specific databases

    CTDi 5449.
    RGDi 3367. Pou1f1.

    Phylogenomic databases

    eggNOGi NOG241031.
    GeneTreei ENSGT00730000110809.
    HOVERGENi HBG053120.
    KOi K09363.
    OMAi TATGLHY.
    OrthoDBi EOG7DJSMG.
    PhylomeDBi P10037.
    TreeFami TF316413.

    Miscellaneous databases

    EvolutionaryTracei P10037.
    PROi P10037.

    Gene expression databases

    Genevestigatori P10037.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR015586. Pit_1.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view ]
    PANTHERi PTHR11636:SF82. PTHR11636:SF82. 1 hit.
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein."
      Bodner M., Castrillo J.-L., Theill L.E., Deerinck T., Ellisman M., Karin M.
      Cell 55:505-518(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    2. "A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype."
      Ingraham H.A., Chen R., Mangalam H.J., Elsholtz H.P., Flynn S.E., Lin C.R., Simmons D.M., Swanson L., Rosenfeld M.G.
      Cell 55:519-529(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    3. "Differential splicing of the GHF1 primary transcript gives rise to two functionally distinct homeodomain proteins."
      Theill L.E., Hattori K., Lazzaro D., Castrillo J.-L., Karin M.
      EMBO J. 11:2261-2269(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
    4. "Functional isoforms of Pit-1 generated by alternative messenger RNA splicing."
      Konzak K.E., Moore D.D.
      Mol. Endocrinol. 6:241-247(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
    5. "An alternatively spliced Pit-1 isoform altered in its ability to trans-activate."
      Morris A.E., Kloss B., McChesney R.E., Bancroft C., Chasin L.A.
      Nucleic Acids Res. 20:1355-1361(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
    6. "Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility."
      Jacobson E.M., Li P., Leon-Del-Rio A., Rosenfeld M.G., Aggarwal A.K.
      Genes Dev. 11:198-212(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 130-273.
      Strain: Sprague-Dawley.
      Tissue: Pituitary.

    Entry informationi

    Entry nameiPIT1_RAT
    AccessioniPrimary (citable) accession number: P10037
    Secondary accession number(s): Q63431
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 153 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3