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Protein

Pituitary-specific positive transcription factor 1

Gene

Pou1f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi214 – 273HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary-specific positive transcription factor 1
Short name:
PIT-1
Alternative name(s):
Growth hormone factor 1
Short name:
GHF-1
Gene namesi
Name:Pou1f1
Synonyms:Ghf-1, Pit-1, Pit1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi3367 Pou1f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007011 – 291Pituitary-specific positive transcription factor 1Add BLAST291

Proteomic databases

PRIDEiP10037

PTM databases

iPTMnetiP10037
PhosphoSitePlusiP10037

Expressioni

Gene expression databases

BgeeiENSRNOG00000000715
GenevisibleiP10037 RN

Interactioni

Subunit structurei

Interacts with PITX1. Interacts with LHX3. Interacts with ELK1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PITX2Q99697-32EBI-9825525,EBI-1175243From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247549, 4 interactors
CORUMiP10037
IntActiP10037, 1 interactor

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi129 – 146Combined sources18
Helixi150 – 159Combined sources10
Helixi167 – 174Combined sources8
Helixi180 – 196Combined sources17
Helixi223 – 235Combined sources13
Helixi241 – 251Combined sources11
Helixi255 – 268Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AU7X-ray2.30A/B128-273[»]
ProteinModelPortaliP10037
SMRiP10037
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10037

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 198POU-specificPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

GeneTreeiENSGT00760000118935
HOVERGENiHBG053120
InParanoidiP10037
OMAiMSCQPFT
OrthoDBiEOG091G0U0S
PhylomeDBiP10037
TreeFamiTF316413

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR015586 Pit_1
IPR013847 POU
IPR000327 POU_dom
PANTHERiPTHR11636:SF84 PTHR11636:SF84, 3 hits
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P10037-1) [UniParc]FASTAAdd to basket
Also known as: PIT-1B, GHF-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCQPFTSAD TFIPLNSDAS AALPLRMHHS AAEGLPASNH ATNVMSTATG
60 70 80 90 100
LHYSVPSCHY GNQPSTYGVM AGTLTPCLYK FPDHTLSHGF PPLHQPLLAE
110 120 130 140 150
DPTASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ
160 170 180 190 200
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV
210 220 230 240 250
GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEHSKPS SQEIMRMAEE
260 270 280 290
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R
Length:291
Mass (Da):32,903
Last modified:April 27, 2001 - v3
Checksum:i2651876B2F7668A1
GO
Isoform A (identifier: P10037-2) [UniParc]FASTAAdd to basket
Also known as: PIT-1A, GHF-2

The sequence of this isoform differs from the canonical sequence as follows:
     47-47: T → TVPSILSLIQTPKCLHTYFSMTTMGNT

Note: Altered in its ability to trans-activate compared to isoform B.
Show »
Length:317
Mass (Da):35,770
Checksum:iF217B4F2307D7940
GO

Sequence cautioni

The sequence AAA41851 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAA41853 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00231647T → TVPSILSLIQTPKCLHTYFS MTTMGNT in isoform A. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65364
, X65365, X65367, X65368 Genomic DNA Translation: CAA46440.1
L01506 mRNA Translation: AAA41850.1
L01506 mRNA Translation: AAA41851.1 Different initiation.
L01507 mRNA Translation: AAA41852.1
L01507 mRNA Translation: AAA41853.1 Different initiation.
S97398 mRNA Translation: AAC60656.1
M23253 mRNA Translation: AAA41854.1
X12658 mRNA Translation: CAA31185.1
X63089 mRNA No translation available.
PIRiS22705
RefSeqiXP_006248036.1, XM_006247974.1 [P10037-2]
UniGeneiRn.10445

Genome annotation databases

EnsembliENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715 [P10037-1]
GeneIDi25517
UCSCiRGD:3367 rat [P10037-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPIT1_RAT
AccessioniPrimary (citable) accession number: P10037
Secondary accession number(s): Q63431
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 27, 2001
Last modified: May 23, 2018
This is version 181 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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