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P10037 (PIT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 152. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pituitary-specific positive transcription factor 1

Short name=PIT-1
Alternative name(s):
Growth hormone factor 1
Short name=GHF-1
Gene names
Name:Pou1f1
Synonyms:Ghf-1, Pit-1, Pit1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the POU transcription factor family. Class-1 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence caution

The sequence AAA41851.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAA41853.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell differentiation

Inferred from electronic annotation. Source: Ensembl

adenohypophysis development

Inferred from mutant phenotype PubMed 16216912. Source: RGD

cell fate specification

Inferred from electronic annotation. Source: Ensembl

determination of adult lifespan

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 17021049. Source: RGD

nuclear transport

Inferred from mutant phenotype PubMed 16030140. Source: RGD

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of inositol trisphosphate biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of multicellular organism growth

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 8370519. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 8370519. Source: UniProtKB

regulation of insulin-like growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

somatotropin secreting cell development

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 16030140. Source: RGD

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II transcription coactivator activity

Inferred from electronic annotation. Source: Ensembl

chromatin binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 16030140. Source: RGD

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

transcription corepressor activity

Inferred from mutant phenotype PubMed 16030140. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: P10037-1)

Also known as: PIT-1B; GHF-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: P10037-2)

Also known as: PIT-1A; GHF-2;

The sequence of this isoform differs from the canonical sequence as follows:
     47-47: T → TVPSILSLIQTPKCLHTYFSMTTMGNT
Note: Altered in its ability to trans-activate compared to isoform B.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Pituitary-specific positive transcription factor 1
PRO_0000100701

Regions

Domain124 – 19875POU-specific
DNA binding214 – 27360Homeobox

Natural variations

Alternative sequence471T → TVPSILSLIQTPKCLHTYFS MTTMGNT in isoform A.
VSP_002316

Secondary structure

............... 291
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform B (PIT-1B) (GHF-1) [UniParc].

Last modified April 27, 2001. Version 3.
Checksum: 2651876B2F7668A1

FASTA29132,903
        10         20         30         40         50         60 
MSCQPFTSAD TFIPLNSDAS AALPLRMHHS AAEGLPASNH ATNVMSTATG LHYSVPSCHY 

        70         80         90        100        110        120 
GNQPSTYGVM AGTLTPCLYK FPDHTLSHGF PPLHQPLLAE DPTASEFKQE LRRKSKLVEE 

       130        140        150        160        170        180 
PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF 

       190        200        210        220        230        240 
KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEHSKPS 

       250        260        270        280        290 
SQEIMRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R 

« Hide

Isoform A (PIT-1A) (GHF-2) [UniParc].

Checksum: F217B4F2307D7940
Show »

FASTA31735,770

References

[1]"The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein."
Bodner M., Castrillo J.-L., Theill L.E., Deerinck T., Ellisman M., Karin M.
Cell 55:505-518(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
[2]"A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype."
Ingraham H.A., Chen R., Mangalam H.J., Elsholtz H.P., Flynn S.E., Lin C.R., Simmons D.M., Swanson L., Rosenfeld M.G.
Cell 55:519-529(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
[3]"Differential splicing of the GHF1 primary transcript gives rise to two functionally distinct homeodomain proteins."
Theill L.E., Hattori K., Lazzaro D., Castrillo J.-L., Karin M.
EMBO J. 11:2261-2269(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[4]"Functional isoforms of Pit-1 generated by alternative messenger RNA splicing."
Konzak K.E., Moore D.D.
Mol. Endocrinol. 6:241-247(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
[5]"An alternatively spliced Pit-1 isoform altered in its ability to trans-activate."
Morris A.E., Kloss B., McChesney R.E., Bancroft C., Chasin L.A.
Nucleic Acids Res. 20:1355-1361(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING.
[6]"Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility."
Jacobson E.M., Li P., Leon-Del-Rio A., Rosenfeld M.G., Aggarwal A.K.
Genes Dev. 11:198-212(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 130-273.
Strain: Sprague-Dawley.
Tissue: Pituitary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65364 expand/collapse EMBL AC list , X65365, X65367, X65368 Genomic DNA. Translation: CAA46440.1.
L01506 mRNA. Translation: AAA41850.1.
L01506 mRNA. Translation: AAA41851.1. Different initiation.
L01507 mRNA. Translation: AAA41852.1.
L01507 mRNA. Translation: AAA41853.1. Different initiation.
S97398 mRNA. Translation: AAC60656.1.
M23253 mRNA. Translation: AAA41854.1.
X12658 mRNA. Translation: CAA31185.1.
X63089 mRNA. No translation available.
PIRS22705.
RefSeqXP_006248036.1. XM_006247974.1. [P10037-2]
UniGeneRn.10445.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AU7X-ray2.30A/B128-273[»]
ProteinModelPortalP10037.
SMRP10037. Positions 129-273.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP10037.

Proteomic databases

PaxDbP10037.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715. [P10037-2]
GeneID25517.
KEGGrno:25517.
UCSCRGD:3367. rat. [P10037-1]

Organism-specific databases

CTD5449.
RGD3367. Pou1f1.

Phylogenomic databases

eggNOGNOG241031.
GeneTreeENSGT00730000110809.
HOVERGENHBG053120.
KOK09363.
OMATATGLHY.
OrthoDBEOG7DJSMG.
PhylomeDBP10037.
TreeFamTF316413.

Gene expression databases

GenevestigatorP10037.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10037.
PROP10037.

Entry information

Entry namePIT1_RAT
AccessionPrimary (citable) accession number: P10037
Secondary accession number(s): Q63431
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references