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Protein

Pituitary-specific positive transcription factor 1

Gene

Pou1f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi214 – 27360HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  4. RNA polymerase II transcription coactivator activity Source: Ensembl
  5. transcription corepressor activity Source: RGD

GO - Biological processi

  1. adenohypophysis development Source: RGD
  2. B cell differentiation Source: Ensembl
  3. cell fate specification Source: Ensembl
  4. determination of adult lifespan Source: Ensembl
  5. negative regulation of transcription from RNA polymerase II promoter Source: RGD
  6. nuclear transport Source: RGD
  7. positive regulation of cell proliferation Source: Ensembl
  8. positive regulation of inositol trisphosphate biosynthetic process Source: Ensembl
  9. positive regulation of multicellular organism growth Source: Ensembl
  10. positive regulation of transcription, DNA-templated Source: UniProtKB
  11. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  12. regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
  13. somatotropin secreting cell development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary-specific positive transcription factor 1
Short name:
PIT-1
Alternative name(s):
Growth hormone factor 1
Short name:
GHF-1
Gene namesi
Name:Pou1f1
Synonyms:Ghf-1, Pit-1, Pit1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 11

Organism-specific databases

RGDi3367. Pou1f1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Pituitary-specific positive transcription factor 1PRO_0000100701Add
BLAST

Proteomic databases

PaxDbiP10037.

PTM databases

PhosphoSiteiP10037.

Expressioni

Gene expression databases

ExpressionAtlasiP10037. baseline.
GenevestigatoriP10037.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PITX2Q99697-32EBI-9825525,EBI-1175243From a different organism.

Protein-protein interaction databases

IntActiP10037. 1 interaction.

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi129 – 14618Combined sources
Helixi150 – 15910Combined sources
Helixi167 – 1748Combined sources
Helixi180 – 19617Combined sources
Helixi223 – 23513Combined sources
Helixi241 – 25111Combined sources
Helixi255 – 26814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AU7X-ray2.30A/B128-273[»]
ProteinModelPortaliP10037.
SMRiP10037. Positions 129-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10037.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 19875POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG241031.
GeneTreeiENSGT00760000118935.
HOVERGENiHBG053120.
InParanoidiP10037.
KOiK09363.
OMAiTATGLHY.
OrthoDBiEOG7DJSMG.
PhylomeDBiP10037.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P10037-1) [UniParc]FASTAAdd to basket

Also known as: PIT-1B, GHF-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCQPFTSAD TFIPLNSDAS AALPLRMHHS AAEGLPASNH ATNVMSTATG
60 70 80 90 100
LHYSVPSCHY GNQPSTYGVM AGTLTPCLYK FPDHTLSHGF PPLHQPLLAE
110 120 130 140 150
DPTASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ
160 170 180 190 200
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV
210 220 230 240 250
GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEHSKPS SQEIMRMAEE
260 270 280 290
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R
Length:291
Mass (Da):32,903
Last modified:April 27, 2001 - v3
Checksum:i2651876B2F7668A1
GO
Isoform A (identifier: P10037-2) [UniParc]FASTAAdd to basket

Also known as: PIT-1A, GHF-2

The sequence of this isoform differs from the canonical sequence as follows:
     47-47: T → TVPSILSLIQTPKCLHTYFSMTTMGNT

Note: Altered in its ability to trans-activate compared to isoform B.

Show »
Length:317
Mass (Da):35,770
Checksum:iF217B4F2307D7940
GO

Sequence cautioni

The sequence AAA41851.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAA41853.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei47 – 471T → TVPSILSLIQTPKCLHTYFS MTTMGNT in isoform A. CuratedVSP_002316

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65364
, X65365, X65367, X65368 Genomic DNA. Translation: CAA46440.1.
L01506 mRNA. Translation: AAA41850.1.
L01506 mRNA. Translation: AAA41851.1. Different initiation.
L01507 mRNA. Translation: AAA41852.1.
L01507 mRNA. Translation: AAA41853.1. Different initiation.
S97398 mRNA. Translation: AAC60656.1.
M23253 mRNA. Translation: AAA41854.1.
X12658 mRNA. Translation: CAA31185.1.
X63089 mRNA. No translation available.
PIRiS22705.
RefSeqiXP_006248036.1. XM_006247974.1. [P10037-2]
UniGeneiRn.10445.

Genome annotation databases

EnsembliENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715. [P10037-2]
GeneIDi25517.
KEGGirno:25517.
UCSCiRGD:3367. rat. [P10037-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65364
, X65365, X65367, X65368 Genomic DNA. Translation: CAA46440.1.
L01506 mRNA. Translation: AAA41850.1.
L01506 mRNA. Translation: AAA41851.1. Different initiation.
L01507 mRNA. Translation: AAA41852.1.
L01507 mRNA. Translation: AAA41853.1. Different initiation.
S97398 mRNA. Translation: AAC60656.1.
M23253 mRNA. Translation: AAA41854.1.
X12658 mRNA. Translation: CAA31185.1.
X63089 mRNA. No translation available.
PIRiS22705.
RefSeqiXP_006248036.1. XM_006247974.1. [P10037-2]
UniGeneiRn.10445.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AU7X-ray2.30A/B128-273[»]
ProteinModelPortaliP10037.
SMRiP10037. Positions 129-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10037. 1 interaction.

PTM databases

PhosphoSiteiP10037.

Proteomic databases

PaxDbiP10037.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715. [P10037-2]
GeneIDi25517.
KEGGirno:25517.
UCSCiRGD:3367. rat. [P10037-1]

Organism-specific databases

CTDi5449.
RGDi3367. Pou1f1.

Phylogenomic databases

eggNOGiNOG241031.
GeneTreeiENSGT00760000118935.
HOVERGENiHBG053120.
InParanoidiP10037.
KOiK09363.
OMAiTATGLHY.
OrthoDBiEOG7DJSMG.
PhylomeDBiP10037.
TreeFamiTF316413.

Miscellaneous databases

EvolutionaryTraceiP10037.
PROiP10037.

Gene expression databases

ExpressionAtlasiP10037. baseline.
GenevestigatoriP10037.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein."
    Bodner M., Castrillo J.-L., Theill L.E., Deerinck T., Ellisman M., Karin M.
    Cell 55:505-518(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
  2. "A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype."
    Ingraham H.A., Chen R., Mangalam H.J., Elsholtz H.P., Flynn S.E., Lin C.R., Simmons D.M., Swanson L., Rosenfeld M.G.
    Cell 55:519-529(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
  3. "Differential splicing of the GHF1 primary transcript gives rise to two functionally distinct homeodomain proteins."
    Theill L.E., Hattori K., Lazzaro D., Castrillo J.-L., Karin M.
    EMBO J. 11:2261-2269(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
  4. "Functional isoforms of Pit-1 generated by alternative messenger RNA splicing."
    Konzak K.E., Moore D.D.
    Mol. Endocrinol. 6:241-247(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
  5. "An alternatively spliced Pit-1 isoform altered in its ability to trans-activate."
    Morris A.E., Kloss B., McChesney R.E., Bancroft C., Chasin L.A.
    Nucleic Acids Res. 20:1355-1361(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  6. "Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility."
    Jacobson E.M., Li P., Leon-Del-Rio A., Rosenfeld M.G., Aggarwal A.K.
    Genes Dev. 11:198-212(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 130-273.
    Strain: Sprague-Dawley.
    Tissue: Pituitary.

Entry informationi

Entry nameiPIT1_RAT
AccessioniPrimary (citable) accession number: P10037
Secondary accession number(s): Q63431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 27, 2001
Last modified: March 4, 2015
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.