Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Relaxosome protein TraM

Gene

traM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Cooperatively binds 3 regions in the F plasmid oriT locus; 2 are required for autoregulation while the other is required for plasmid transfer. Bends oriT DNA less than 50 degrees. Plasmid specificity is conferred by the TraD-TraM pair.4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Conjugation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Relaxosome protein TraM
Gene namesi
Name:traM
Ordered Locus Names:ECOK12F071
Encoded oniPlasmid F12 Publications
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of conjugative DNA transfer.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5N → D: Loss of all functions. 1 Publication1
Mutagenesisi76K → E: 100,000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. 1 Publication1
Mutagenesisi88E → L or Q: Increased homotetramer stability. 1 Publication1
Mutagenesisi99K → E: 100,000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. Binds less well to TraD. May be dominant over wild-type. Partially rescued by a TraD E-712 mutation. 2 Publications1
Mutagenesisi106V → A: 2000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. 1 Publication1
Mutagenesisi110R → E: 33,000-fold decrease in conjugation efficiency, but tetramerize and bind DNA normally. 1 Publication1
Mutagenesisi121F → S: Alters oligomerization, probably more dimers than tetramers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000684671 – 127Relaxosome protein TraMAdd BLAST127

Expressioni

Inductioni

Regulates its own expression from two promoters, requires TraY as well as expression of the tra operon for maximal transcription. TraM is in a monocistonic operon.1 Publication

Interactioni

Subunit structurei

Homotetramer. 2 homotetramers cooperatively bind to DNA although they do not contact each other; cooperativity is achieved by DNA kinking and unwinding (Probable). Part of the relaxosome, a complex composed of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to the F plasmid origin of transfer (oriT) in a site- and sequence-specific manner. Interacts with TraD. Also interacts with TraY.Curated6 Publications

Protein-protein interaction databases

DIPiDIP-27653N.

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi10 – 24Combined sources15
Beta strandi30 – 33Combined sources4
Helixi35 – 53Combined sources19
Helixi63 – 89Combined sources27
Helixi92 – 94Combined sources3
Helixi98 – 100Combined sources3
Helixi102 – 120Combined sources19
Beta strandi123 – 125Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G7OX-ray1.40A58-127[»]
2G9EX-ray1.80A58-127[»]
3D8AX-ray2.55A/B/C/D/E/F/G/H58-127[»]
4QPOX-ray2.00A/B/C/D2-54[»]
4QPQX-ray3.11A/B/C/D/E/F/G/H2-54[»]
ProteinModelPortaliP10026.
SMRiP10026.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10026.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili63 – 891 PublicationAdd BLAST27

Sequence similaritiesi

Belongs to the relaxosome TraM family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

OMAiSVEMERF.

Family and domain databases

CDDicd14804. Tra_M. 1 hit.
InterProiIPR010992. IHF-like_DNA-bd_dom.
IPR007925. TRelaxosome_TraM.
[Graphical view]
PfamiPF05261. Tra_M. 1 hit.
[Graphical view]
ProDomiPD008204. T4SS_TraM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47729. SSF47729. 1 hit.

Sequencei

Sequence statusi: Complete.

P10026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVNLYISN DAYEKINAII EKRRQEGARE KDVSFSATAS MLLELGLRVH
60 70 80 90 100
EAQMERKESA FNQTEFNKLL LECVVKTQSS VAKILGIESL SPHVSGNSKF
110 120
EYANMVEDIR EKVSSEMERF FPKNDDE
Length:127
Mass (Da):14,507
Last modified:July 1, 1989 - v1
Checksum:iFC4642125B7E7CAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01147 Genomic DNA. Translation: AAA24907.1.
X00545 Genomic DNA. Translation: CAA25216.1.
U01159 Genomic DNA. Translation: AAC44191.1.
AP001918 Genomic DNA. Translation: BAA97941.1.
PIRiA29331. JCECMF.
RefSeqiNP_061450.1. NC_002483.1.
WP_001151527.1. NZ_CP014273.1.

Genome annotation databases

GeneIDi1263565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01147 Genomic DNA. Translation: AAA24907.1.
X00545 Genomic DNA. Translation: CAA25216.1.
U01159 Genomic DNA. Translation: AAC44191.1.
AP001918 Genomic DNA. Translation: BAA97941.1.
PIRiA29331. JCECMF.
RefSeqiNP_061450.1. NC_002483.1.
WP_001151527.1. NZ_CP014273.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G7OX-ray1.40A58-127[»]
2G9EX-ray1.80A58-127[»]
3D8AX-ray2.55A/B/C/D/E/F/G/H58-127[»]
4QPOX-ray2.00A/B/C/D2-54[»]
4QPQX-ray3.11A/B/C/D/E/F/G/H2-54[»]
ProteinModelPortaliP10026.
SMRiP10026.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-27653N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1263565.

Phylogenomic databases

OMAiSVEMERF.

Miscellaneous databases

EvolutionaryTraceiP10026.
PROiP10026.

Family and domain databases

CDDicd14804. Tra_M. 1 hit.
InterProiIPR010992. IHF-like_DNA-bd_dom.
IPR007925. TRelaxosome_TraM.
[Graphical view]
PfamiPF05261. Tra_M. 1 hit.
[Graphical view]
ProDomiPD008204. T4SS_TraM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47729. SSF47729. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRAM1_ECOLI
AccessioniPrimary (citable) accession number: P10026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.