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Protein

Potassium channel toxin alpha-KTx 23.3

Gene
N/A
Organism
Scorpiops tibetanus (Scorpion)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This toxin shows both immunosuppressive and anti-inflammatory activities (PubMed:28077339). It has the potential to inhibit human T cell activation, since it reduces IL-2 secretion and the expression of T cell activation marker CD69 and acts as a antiinflammatory agent, since it provokes the reduction of secretion of both IFN-gamma and TNF-alpha (PubMed:28077339). In vivo, the delayed-type hypersensitivity response in rat autoimmune disease model is ameliorated in the presence of this toxin (PubMed:28077339). Acts by blocking Kv1.3/KCNA3 potassium channels of T-lymphocytes (By similarity).By similarity1 Publication

Miscellaneous

Recombinant toxin does not cause hemolysis in human erythrocytes.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei45Basic residue of the functional dyad1
Sitei45Key residue for immunosuppressive and antiinflammatory activities1 Publication1
Sitei54Aromatic residue of the functional dyad1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 23.31 Publication
Alternative name(s):
Toxin St201 Publication
OrganismiScorpiops tibetanus (Scorpion)
Taxonomic identifieri500600 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaChactoideaEuscorpiidaeScorpiopinaeScorpiopiniScorpiops

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45K → A: Complete loss of inhibition potency on human T cells (IL-2, TNF-alpha and IFN-gamma secretion). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000043989724 – 57Potassium channel toxin alpha-KTx 23.31 PublicationAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 46By similarity
Disulfide bondi32 ↔ 51By similarity
Disulfide bondi36 ↔ 53By similarity
Disulfide bondi41 ↔ 56By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Family & Domainsi

Domaini

Has the CSalpha/beta fold, which comprises one or two short alpha helices connected to anti-parallel beta-sheets stabilized by three or four disulfide bonds.By similarity

Sequence similaritiesi

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 23 subfamily.1 Publication

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiView protein in InterPro
IPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
PfamiView protein in Pfam
PF00451. Toxin_2. 1 hit.
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD003586. Scorpion_toxinS. 1 hit.
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DP36-1 [UniParc]FASTAAdd to basket

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MKMSIVIILL LFTCLIATNG ASGTKCSGSP ECVKFCRTKG CRNGKCMNRS

CKCYLCS
Length:57
Mass (Da):6,175
Last modified:May 10, 2017 - v1
Checksum:iC9391064ED2EEABE
GO

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiKA233_SCOTI
AccessioniPrimary (citable) accession number: P0DP36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2017
Last sequence update: May 10, 2017
Last modified: July 5, 2017
This is version 3 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Documents

  1. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  2. SIMILARITY comments
    Index of protein domains and families