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Protein

Sulfotransferase 1A3

Gene

SULT1A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

Catalytic activityi

3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86Substrate1
Active sitei108Proton acceptorBy similarity1
Binding sitei130PAPS1
Binding sitei138PAPS1
Binding sitei146Substrate1
Binding sitei193PAPS1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 53PAPS6
Nucleotide bindingi227 – 232PAPS6
Nucleotide bindingi257 – 259PAPS3

GO - Molecular functioni

  • aryl sulfotransferase activity Source: CACAO
  • sulfotransferase activity Source: BHF-UCL

GO - Biological processi

  • 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: Reactome
  • catecholamine metabolic process Source: UniProtKB-KW
  • flavonoid metabolic process Source: BHF-UCL
  • IRE1-mediated unfolded protein response Source: Reactome
  • steroid metabolic process Source: UniProtKB-KW
  • sulfation Source: BHF-UCL
  • xenobiotic metabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Catecholamine metabolism, Lipid metabolism, Steroid metabolism

Enzyme and pathway databases

ReactomeiR-HSA-156584. Cytosolic sulfonation of small molecules.
R-HSA-381038. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfotransferase 1A3 (EC:2.8.2.1)
Short name:
ST1A3
Alternative name(s):
Aryl sulfotransferase 1A3/1A4
Catecholamine-sulfating phenol sulfotransferase
HAST3
M-PST
Monoamine-sulfating phenol sulfotransferase
Placental estrogen sulfotransferase
Sulfotransferase 1A3/1A4
Sulfotransferase, monoamine-preferring
Thermolabile phenol sulfotransferase
Short name:
TL-PST
Gene namesi
Name:SULT1A3
Synonyms:STM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:11455. SULT1A3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi445329.
6818.
OpenTargetsiENSG00000213648.
ENSG00000261052.

Chemistry databases

ChEMBLiCHEMBL1743293.

Polymorphism and mutation databases

DMDMi1711609.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851591 – 295Sulfotransferase 1A3Add BLAST295

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP0DMM9.
PeptideAtlasiP0DMM9.
PRIDEiP0DMM9.
TopDownProteomicsiP0DMM9-2. [P0DMM9-2]

PTM databases

iPTMnetiP0DMM9.
PhosphoSitePlusiP0DMM9.

Expressioni

Tissue specificityi

Liver, colon, kidney, lung, brain, spleen, small intestine, placenta and leukocyte.

Gene expression databases

CleanExiHS_SULT1A3.
HS_SULT1A4.
ExpressionAtlasiP0DMM9. baseline and differential.
GenevisibleiP0DMM9. HS.

Organism-specific databases

HPAiHPA049500.
HPA051051.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KHDRBS2Q5VWX13EBI-10196922,EBI-742808
NIF3L1Q9GZT83EBI-10196922,EBI-740897
SHMT1P348963EBI-10196922,EBI-715117

Protein-protein interaction databases

BioGridi112687. 12 interactors.
138644. 4 interactors.
IntActiP0DMM9. 4 interactors.
STRINGi9606.ENSP00000343645.

Structurei

Secondary structure

1295
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 15Combined sources4
Beta strandi18 – 21Combined sources4
Helixi22 – 27Combined sources6
Helixi30 – 33Combined sources4
Beta strandi41 – 45Combined sources5
Helixi51 – 62Combined sources12
Turni63 – 65Combined sources3
Helixi67 – 70Combined sources4
Helixi75 – 78Combined sources4
Turni95 – 98Combined sources4
Beta strandi104 – 107Combined sources4
Helixi111 – 113Combined sources3
Helixi116 – 120Combined sources5
Beta strandi124 – 129Combined sources6
Helixi132 – 145Combined sources14
Helixi155 – 163Combined sources9
Helixi172 – 182Combined sources11
Turni183 – 185Combined sources3
Beta strandi188 – 192Combined sources5
Helixi193 – 198Combined sources6
Helixi200 – 211Combined sources12
Helixi217 – 226Combined sources10
Helixi229 – 234Combined sources6
Turni236 – 238Combined sources3
Turni245 – 247Combined sources3
Turni250 – 252Combined sources3
Helixi264 – 266Combined sources3
Helixi270 – 283Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CJMX-ray2.40A1-295[»]
2A3RX-ray2.60A/B1-295[»]
DisProtiDP00011.
ProteinModelPortaliP0DMM9.
SMRiP0DMM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 108Substrate binding3

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
GeneTreeiENSGT00760000118932.
HOVERGENiHBG001195.
KOiK01014.
OMAiTSFREMK.
PhylomeDBiP0DMM9.
TreeFamiTF321745.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P0DMM9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELIQDTSRP PLEYVKGVPL IKYFAEALGP LQSFQARPDD LLINTYPKSG
60 70 80 90 100
TTWVSQILDM IYQGGDLEKC NRAPIYVRVP FLEVNDPGEP SGLETLKDTP
110 120 130 140 150
PPRLIKSHLP LALLPQTLLD QKVKVVYVAR NPKDVAVSYY HFHRMEKAHP
160 170 180 190 200
EPGTWDSFLE KFMAGEVSYG SWYQHVQEWW ELSRTHPVLY LFYEDMKENP
210 220 230 240 250
KREIQKILEF VGRSLPEETM DFMVQHTSFK EMKKNPMTNY TTVPQELMDH
260 270 280 290
SISPFMRKGM AGDWKTTFTV AQNERFDADY AEKMAGCSLS FRSEL
Length:295
Mass (Da):34,196
Last modified:September 3, 2014 - v1
Checksum:iECDDEC03DBE30D46
GO
Isoform 2 (identifier: P0DMM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-295: VSYGSWYQHV...GCSLSFRSEL → GGLDWRKEGVKPRGGGYNVQQPCVGAPCPLL

Note: No experimental confirmation available.Curated
Show »
Length:197
Mass (Da):22,069
Checksum:i44C5F13EDA71BC1D
GO
Isoform 3 (identifier: P0DMM9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-198: E → EEPSAAQ

Show »
Length:301
Mass (Da):34,780
Checksum:i7018229AD3F67542
GO

Sequence cautioni

Isoform 2 : The sequence BAC85507 differs from that shown. Reason: Frameshift at position 101.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti220 – 225MDFMVQ → VDLMVE in AAC99987 (Ref. 8) Curated6
Sequence conflicti235N → T in AAC99987 (Ref. 8) Curated1
Sequence conflicti244 – 245PQ → RR in AAC99987 (Ref. 8) Curated2
Sequence conflicti290S → T in AAC99987 (Ref. 8) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_071108101P → H Decreases levels of sulfotransferase activity. 1 Publication1
Natural variantiVAR_071109101P → L Decreases levels of sulfotransferase activity. 1 PublicationCorresponds to variant rs751527244dbSNPEnsembl.1
Natural variantiVAR_071110144R → C No effect on sulfotransferase activity. 1 Publication1
Natural variantiVAR_071111234K → N Decreases levels of sulfotransferase activity; accelerates proteasome-dependent degradation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012326167 – 295VSYGS…FRSEL → GGLDWRKEGVKPRGGGYNVQ QPCVGAPCPLL in isoform 2. 1 PublicationAdd BLAST129
Alternative sequenceiVSP_047771198E → EEPSAAQ in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19956 mRNA. Translation: AAA02943.1.
L25275 mRNA. Translation: AAA36523.1.
L19956 mRNA. Translation: AAA17723.1.
U20499 Genomic DNA. Translation: AAA64490.1.
X84653 mRNA. Translation: CAA59146.1.
L34160 Genomic DNA. No translation available.
U37686 Genomic DNA. Translation: AAA86536.1.
U34199 mRNA. Translation: AAC99987.1.
AK122733 mRNA. Translation: BAC85507.1. Frameshift.
AK298073 mRNA. Translation: BAG60362.1.
AC106782 Genomic DNA. No translation available.
AC133555 Genomic DNA. No translation available.
AB111094 mRNA. Translation: BAE94928.1.
BC014471 mRNA. Translation: AAH14471.1.
BC078144 mRNA. Translation: AAH78144.1.
U08099 Genomic DNA. Translation: AAA82126.1.
CCDSiCCDS10674.1. [P0DMM9-1]
PIRiA55451.
RefSeqiNP_001017390.1. NM_001017390.2. [P0DMM9-1]
NP_808220.1. NM_177552.3. [P0DMM9-1]
XP_011506903.1. XM_011508601.1. [P0DMM9-3]
XP_016855108.1. XM_016999619.1. [P0DMM9-1]
UniGeneiHs.460558.
Hs.744871.

Genome annotation databases

EnsembliENST00000338971; ENSP00000343645; ENSG00000261052. [P0DMM9-1]
ENST00000395138; ENSP00000378570; ENSG00000261052. [P0DMM9-1]
ENST00000563322; ENSP00000454518; ENSG00000261052. [P0DMM9-3]
GeneIDi105369243.
445329.
6818.
KEGGihsa:105369243.
hsa:445329.
hsa:6818.
UCSCiuc010bzt.4. human. [P0DMM9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19956 mRNA. Translation: AAA02943.1.
L25275 mRNA. Translation: AAA36523.1.
L19956 mRNA. Translation: AAA17723.1.
U20499 Genomic DNA. Translation: AAA64490.1.
X84653 mRNA. Translation: CAA59146.1.
L34160 Genomic DNA. No translation available.
U37686 Genomic DNA. Translation: AAA86536.1.
U34199 mRNA. Translation: AAC99987.1.
AK122733 mRNA. Translation: BAC85507.1. Frameshift.
AK298073 mRNA. Translation: BAG60362.1.
AC106782 Genomic DNA. No translation available.
AC133555 Genomic DNA. No translation available.
AB111094 mRNA. Translation: BAE94928.1.
BC014471 mRNA. Translation: AAH14471.1.
BC078144 mRNA. Translation: AAH78144.1.
U08099 Genomic DNA. Translation: AAA82126.1.
CCDSiCCDS10674.1. [P0DMM9-1]
PIRiA55451.
RefSeqiNP_001017390.1. NM_001017390.2. [P0DMM9-1]
NP_808220.1. NM_177552.3. [P0DMM9-1]
XP_011506903.1. XM_011508601.1. [P0DMM9-3]
XP_016855108.1. XM_016999619.1. [P0DMM9-1]
UniGeneiHs.460558.
Hs.744871.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CJMX-ray2.40A1-295[»]
2A3RX-ray2.60A/B1-295[»]
DisProtiDP00011.
ProteinModelPortaliP0DMM9.
SMRiP0DMM9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112687. 12 interactors.
138644. 4 interactors.
IntActiP0DMM9. 4 interactors.
STRINGi9606.ENSP00000343645.

Chemistry databases

ChEMBLiCHEMBL1743293.

PTM databases

iPTMnetiP0DMM9.
PhosphoSitePlusiP0DMM9.

Polymorphism and mutation databases

DMDMi1711609.

Proteomic databases

PaxDbiP0DMM9.
PeptideAtlasiP0DMM9.
PRIDEiP0DMM9.
TopDownProteomicsiP0DMM9-2. [P0DMM9-2]

Protocols and materials databases

DNASUi445329.
6818.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338971; ENSP00000343645; ENSG00000261052. [P0DMM9-1]
ENST00000395138; ENSP00000378570; ENSG00000261052. [P0DMM9-1]
ENST00000563322; ENSP00000454518; ENSG00000261052. [P0DMM9-3]
GeneIDi105369243.
445329.
6818.
KEGGihsa:105369243.
hsa:445329.
hsa:6818.
UCSCiuc010bzt.4. human. [P0DMM9-1]

Organism-specific databases

CTDi445329.
6818.
DisGeNETi445329.
6818.
GeneCardsiSULT1A3.
HGNCiHGNC:11455. SULT1A3.
HPAiHPA049500.
HPA051051.
MIMi600641. gene.
neXtProtiNX_P0DMM9.
OpenTargetsiENSG00000213648.
ENSG00000261052.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
GeneTreeiENSGT00760000118932.
HOVERGENiHBG001195.
KOiK01014.
OMAiTSFREMK.
PhylomeDBiP0DMM9.
TreeFamiTF321745.

Enzyme and pathway databases

ReactomeiR-HSA-156584. Cytosolic sulfonation of small molecules.
R-HSA-381038. XBP1(S) activates chaperone genes.

Miscellaneous databases

PROiP0DMM9.
SOURCEiSearch...

Gene expression databases

CleanExiHS_SULT1A3.
HS_SULT1A4.
ExpressionAtlasiP0DMM9. baseline and differential.
GenevisibleiP0DMM9. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiST1A3_HUMAN
AccessioniPrimary (citable) accession number: P0DMM9
Secondary accession number(s): B4DNV0
, O95603, P50224, Q1ET66, Q6ZWJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: September 3, 2014
Last modified: November 30, 2016
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Found in a segmental duplication on p arm of chromosome 16 giving rise to two identical copies of this gene sharing exons with SLX1A and SLX1B. The ORFs of SULT1A3 and SULT1A4 differ with only a single nucleotide change that does not alter the encoded amino acid. It is not possible to determine whether any individual polymorphism is present within SULT1A3 or SULT1A4 (PubMed:15358107).1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.