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Protein

Chorionic somatomammotropin hormone 2

Gene

CSH2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Zinc
Metal bindingi200 – 2001Zinc

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chorionic somatomammotropin hormone 2
Short name:
Choriomammotropin
Alternative name(s):
Lactogen
Placental lactogen
Short name:
PL
Gene namesi
Name:CSH2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:2441. CSH2.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: AgBase
  • extracellular region Source: UniProtKB-SubCell
  • membrane-bounded vesicle Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26262 PublicationsAdd
BLAST
Chaini27 – 217191Chorionic somatomammotropin hormone 2PRO_0000429797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi79 ↔ 1911 Publication
Disulfide bondi208 ↔ 215In monomeric form1 Publication
Disulfide bondi208 – 208Interchain (with C-215); in dimeric form1 Publication
Disulfide bondi215 – 215Interchain (with C-208); in dimeric form1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0DML3.

Expressioni

Gene expression databases

CleanExiHS_CSH2.
ExpressionAtlasiP0DML3. baseline and differential.
GenevisibleiP0DML3. HS.

Organism-specific databases

HPAiHPA043715.

Interactioni

Subunit structurei

Can be found in a monomeric as well as dimeric form.1 Publication

Protein-protein interaction databases

BioGridi107829. 8 interactions.
107830. 9 interactions.
STRINGi9606.ENSP00000376623.

Structurei

3D structure databases

ProteinModelPortaliP0DML3.
SMRiP0DML3. Positions 29-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the somatotropin/prolactin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFR6. Eukaryota.
ENOG4111HU8. LUCA.
GeneTreeiENSGT00730000111012.
KOiK05438.
PhylomeDBiP0DML3.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR001400. Somatotropin.
IPR018116. Somatotropin_CS.
[Graphical view]
PANTHERiPTHR11417. PTHR11417. 1 hit.
PfamiPF00103. Hormone_1. 1 hit.
[Graphical view]
PRINTSiPR00836. SOMATOTROPIN.
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00266. SOMATOTROPIN_1. 1 hit.
PS00338. SOMATOTROPIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: P0DML3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGSRTSLL LAFALLCLPW LQEAGAVQTV PLSRLFDHAM LQAHRAHQLA
60 70 80 90 100
IDTYQEFEET YIPKDQKYSF LHDSQTSFCF SDSIPTPSNM EETQQKSNLE
110 120 130 140 150
LLRISLLLIE SWLEPVRFLR SMFANNLVYD TSDSDDYHLL KDLEEGIQTL
160 170 180 190 200
MGRLEDGSRR TGQILKQTYS KFDTNSHNHD ALLKNYGLLY CFRKDMDKVE
210
TFLRMVQCRS VEGSCGF
Length:217
Mass (Da):24,994
Last modified:July 9, 2014 - v1
Checksum:i39FAACDDB6B2E951
GO
Isoform 2 (identifier: P0DML3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-217: RLEDGSRRTG...CRSVEGSCGF → VRVAPGVANPGTPLA

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:167
Mass (Da):18,804
Checksum:i42E5D3AA748B0FF9
GO
Isoform 3 (identifier: P0DML3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-152: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:122
Mass (Da):13,914
Checksum:i9D3B0B0C811BB619
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841I → T AA sequence (PubMed:5286363).Curated
Sequence conflicti95 – 951Missing AA sequence (PubMed:5286363).Curated
Sequence conflicti116 – 1161Missing AA sequence (PubMed:5286363).Curated
Sequence conflicti134 – 1363SDD → BBS AA sequence (PubMed:5286363).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti104 – 1052IS → L.
VAR_007167

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei58 – 15295Missing in isoform 3. CuratedVSP_055244Add
BLAST
Alternative sequencei153 – 21765RLEDG…GSCGF → VRVAPGVANPGTPLA in isoform 2. CuratedVSP_055245Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00573 mRNA. Translation: CAA23836.1.
J00289 Genomic DNA. Translation: AAA98747.1.
K02401 Genomic DNA. Translation: AAA52115.1.
M15894 Genomic DNA. Translation: AAA52116.1.
J03071 Genomic DNA. Translation: AAA52553.1.
EU421716 Genomic DNA. Translation: ABZ88723.1.
BC022044 mRNA. Translation: AAH22044.1.
BC035965 mRNA. Translation: AAH35965.1.
BC119748 mRNA. Translation: AAI19749.1.
CCDSiCCDS11646.1. [P0DML3-2]
CCDS42368.1. [P0DML3-3]
CCDS42369.1. [P0DML3-1]
PIRiA26449.
E32435.
RefSeqiNP_001308.1. NM_001317.5.
NP_066271.1. NM_020991.3. [P0DML3-1]
UniGeneiHs.654390.
Hs.654515.

Genome annotation databases

EnsembliENST00000336844; ENSP00000338816; ENSG00000213218. [P0DML3-2]
ENST00000345366; ENSP00000308396; ENSG00000213218. [P0DML3-3]
ENST00000392886; ENSP00000376623; ENSG00000213218. [P0DML3-1]
GeneIDi1442.
1443.
KEGGihsa:1442.
hsa:1443.
UCSCiuc002jcg.3. human. [P0DML3-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00573 mRNA. Translation: CAA23836.1.
J00289 Genomic DNA. Translation: AAA98747.1.
K02401 Genomic DNA. Translation: AAA52115.1.
M15894 Genomic DNA. Translation: AAA52116.1.
J03071 Genomic DNA. Translation: AAA52553.1.
EU421716 Genomic DNA. Translation: ABZ88723.1.
BC022044 mRNA. Translation: AAH22044.1.
BC035965 mRNA. Translation: AAH35965.1.
BC119748 mRNA. Translation: AAI19749.1.
CCDSiCCDS11646.1. [P0DML3-2]
CCDS42368.1. [P0DML3-3]
CCDS42369.1. [P0DML3-1]
PIRiA26449.
E32435.
RefSeqiNP_001308.1. NM_001317.5.
NP_066271.1. NM_020991.3. [P0DML3-1]
UniGeneiHs.654390.
Hs.654515.

3D structure databases

ProteinModelPortaliP0DML3.
SMRiP0DML3. Positions 29-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107829. 8 interactions.
107830. 9 interactions.
STRINGi9606.ENSP00000376623.

Proteomic databases

PaxDbiP0DML3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336844; ENSP00000338816; ENSG00000213218. [P0DML3-2]
ENST00000345366; ENSP00000308396; ENSG00000213218. [P0DML3-3]
ENST00000392886; ENSP00000376623; ENSG00000213218. [P0DML3-1]
GeneIDi1442.
1443.
KEGGihsa:1442.
hsa:1443.
UCSCiuc002jcg.3. human. [P0DML3-1]

Organism-specific databases

CTDi1442.
1443.
GeneCardsiCSH2.
HGNCiHGNC:2441. CSH2.
HPAiHPA043715.
MIMi118820. gene.
neXtProtiNX_P0DML3.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFR6. Eukaryota.
ENOG4111HU8. LUCA.
GeneTreeiENSGT00730000111012.
KOiK05438.
PhylomeDBiP0DML3.

Miscellaneous databases

ChiTaRSiCSH2. human.
PROiP0DML3.
SOURCEiSearch...

Gene expression databases

CleanExiHS_CSH2.
ExpressionAtlasiP0DML3. baseline and differential.
GenevisibleiP0DML3. HS.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR001400. Somatotropin.
IPR018116. Somatotropin_CS.
[Graphical view]
PANTHERiPTHR11417. PTHR11417. 1 hit.
PfamiPF00103. Hormone_1. 1 hit.
[Graphical view]
PRINTSiPR00836. SOMATOTROPIN.
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00266. SOMATOTROPIN_1. 1 hit.
PS00338. SOMATOTROPIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The human growth hormone gene locus: structure, evolution, and allelic variations."
    Hirt H., Kimelman J., Birnbaum M.J., Chen E.Y., Seeburg P.H., Eberhardt N.L., Barta A.
    DNA 6:59-70(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Two structurally different genes produce the same secreted human placental lactogen hormone."
    Barrera-Saldana H.A., Seeburg P.H., Saunders G.F.
    J. Biol. Chem. 258:3787-3793(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
  3. "The human growth hormone locus: nucleotide sequence, biology, and evolution."
    Chen E.Y., Liao Y.C., Smith D.H., Barrera-Saldana H.A., Gelinas R.E., Seeburg P.H.
    Genomics 4:479-497(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The human growth hormone gene family: nucleotide sequences show recent divergence and predict a new polypeptide hormone."
    Seeburg P.H.
    DNA 1:239-249(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
  5. "Complex signatures of locus-specific selective pressures and gene conversion on human growth hormone/chorionic somatomammotropin genes."
    Sedman L., Padhukasahasram B., Kelgo P., Laan M.
    Hum. Mutat. 29:1181-1193(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta and Uterus.
  8. "Construction and analysis of recombinant DNA for human chorionic somatomammotropin."
    Shine J., Seeburg P.H., Martial J.A., Baxter J.D., Goodman H.M.
    Nature 270:494-499(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-217 (ISOFORM 1).
  9. "Amino acid sequence of human chorionic somatomammotropin."
    Li C.H., Dixon J.S., Chung D.
    Arch. Biochem. Biophys. 155:95-110(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-217.
  10. "Amino-acid sequence of human placental lactogen."
    Sherwood L.M., Handwerger S., McLaurin W.D., Lanner M.
    Nature New Biol. 233:59-61(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-217.
  11. Erratum
    Sherwood L.M., Handwerger S., McLaurin W.D., Lanner M.
    Nature New Biol. 235:64-64(1972)
  12. "Identification of the interchain disulfide bonds of dimeric human placental lactogen."
    Schneider A.B., Kowalski K., Russell J., Sherwood L.M.
    J. Biol. Chem. 254:3782-3787(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERCHAIN DISULFIDE BONDS, SUBUNIT.
  13. "Crystal structure and site 1 binding energetics of human placental lactogen."
    Walsh S.T., Kossiakoff A.A.
    J. Mol. Biol. 358:773-784(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ZINC-BINDING SITES, DISULFIDE BONDS.

Entry informationi

Entry nameiCSH2_HUMAN
AccessioniPrimary (citable) accession number: P0DML3
Secondary accession number(s): P01243, Q0VDB1, Q14407
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: July 9, 2014
Last modified: May 11, 2016
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

CSH2 sequence only differs from CSH1 sequence in 1 aa.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.