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Protein

Transcriptional regulatory protein RcsA

Gene

rcsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Rcs signaling system, which controls transcription of numerous genes. Binds, with RcsB, to the RcsAB box to regulate expression of genes involved in colanic acid capsule synthesis.UniRule annotation2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi155 – 17420H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Capsule biogenesis/degradation, Sensory transduction, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein RcsAUniRule annotation
Alternative name(s):
Colanic acid capsular biosynthesis activation protein A
Gene namesi
Name:rcsAUniRule annotation
Ordered Locus Names:b1951, JW1935
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10820. rcsA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi145 – 1451M → V in rcsA*; increases stability and capsule synthesis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Transcriptional regulatory protein RcsAPRO_0000184178Add
BLAST

Post-translational modificationi

Degraded by the Lon and the HslUV proteases (PubMed:1999391 and PubMed:14766922). Interaction with RcsB protects RcsA from degradation (PubMed:1999391).1 Publication

Proteomic databases

PaxDbiP0DMC9.

Expressioni

Inductioni

Autoregulated. Repressed by H-NS. Induced by the DsrA small regulatory RNA, which inhibits the H-NS mediated transcriptional silencing.2 Publications

Interactioni

Subunit structurei

Interacts with RcsB.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261047. 8 interactions.
IntActiP0DMC9. 29 interactions.
STRINGi511145.b1951.

Structurei

3D structure databases

ProteinModelPortaliP0DMC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 19666HTH luxR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RcsA family.UniRule annotation
Contains 1 HTH luxR-type DNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107TKS. Bacteria.
COG2771. LUCA.
KOiK07781.
OMAiPGVVFIN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00982. RcsA.
InterProiIPR030866. RcsA.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0DMC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIIMDLCS YTRLGLTGYL LSRGVKKREI NDIETVDDLA IACDSQRPSV
60 70 80 90 100
VFINEDCFIH DASNSQRIKL IINQHPNTLF IVFMAIANVH FDEYLLVRKN
110 120 130 140 150
LLISSKSIKP ESLDDILGDI LKKETTITSF LNMPTLSLSR TESSMLRMWM
160 170 180 190 200
AGQGTIQISD QMNIKAKTVS SHKGNIKRKI KTHNKQVIYH VVRLTDNVTN

GIFVNMR
Length:207
Mass (Da):23,516
Last modified:February 19, 2014 - v1
Checksum:i6DAA22097807AF62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58003 Genomic DNA. Translation: AAA82970.1.
U00096 Genomic DNA. Translation: AAC75018.1.
AP009048 Genomic DNA. Translation: BAA15776.1.
U17137 Genomic DNA. Translation: AAC43315.1.
PIRiA37320.
RefSeqiNP_416461.1. NC_000913.3.
WP_000104001.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75018; AAC75018; b1951.
BAA15776; BAA15776; BAA15776.
GeneIDi946467.
KEGGiecj:JW1935.
eco:b1951.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58003 Genomic DNA. Translation: AAA82970.1.
U00096 Genomic DNA. Translation: AAC75018.1.
AP009048 Genomic DNA. Translation: BAA15776.1.
U17137 Genomic DNA. Translation: AAC43315.1.
PIRiA37320.
RefSeqiNP_416461.1. NC_000913.3.
WP_000104001.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0DMC9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261047. 8 interactions.
IntActiP0DMC9. 29 interactions.
STRINGi511145.b1951.

Proteomic databases

PaxDbiP0DMC9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75018; AAC75018; b1951.
BAA15776; BAA15776; BAA15776.
GeneIDi946467.
KEGGiecj:JW1935.
eco:b1951.

Organism-specific databases

EchoBASEiEB0813.
EcoGeneiEG10820. rcsA.

Phylogenomic databases

eggNOGiENOG4107TKS. Bacteria.
COG2771. LUCA.
KOiK07781.
OMAiPGVVFIN.

Miscellaneous databases

PROiP0DMC9.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00982. RcsA.
InterProiIPR030866. RcsA.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RcsA, an unstable positive regulator of capsular polysaccharide synthesis."
    Stout V., Torres-Cabassa A., Maurizi M.R., Gutnick D., Gottesman S.
    J. Bacteriol. 173:1738-1747(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8, FUNCTION, DEGRADATION BY LON PROTEASE, MUTAGENESIS OF MET-145.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli."
    Sledjeski D., Gottesman S.
    Proc. Natl. Acad. Sci. U.S.A. 92:2003-2007(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20, INDUCTION.
    Strain: K12.
  6. "The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria."
    Wehland M., Bernhard F.
    J. Biol. Chem. 275:7013-7020(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RCSB, INDUCTION, BINDING TO RCSAB BOX.
  7. "Regulation of RcsA by the ClpYQ (HslUV) protease in Escherichia coli."
    Kuo M.S., Chen K.P., Wu W.F.
    Microbiology 150:437-446(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEGRADATION BY HSLUV PROTEASE.

Entry informationi

Entry nameiRCSA_ECOLI
AccessioniPrimary (citable) accession number: P0DMC9
Secondary accession number(s): P24210, P69405, Q47585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: February 19, 2014
Last modified: March 16, 2016
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.